Objective To explore the mechanism by which She-Ti-Zhi-Qiu decoction alleviates allergic rhinitis (AR) through gut microbiota-mediated regulation of T helper cell 17(Th17)/regulatory T cells(Treg) balance and related cytokines. Methods Twenty-eight female SD rats were randomly divided into four groups: the Control group, Model group, STZQ group, and Probiotics group. Except for the Control group, all other groups were sensitized with ovalbumin (OVA) to establish AR models. The Control and Model groups received intragastric administration of normal saline, while the STZQ group was administered She-Ti-Zhi-Qiu Decoction, and Probiotics group received probiotics. After two weeks of continuous intragastric administration, nasal mucosa, serum, peripheral blood, and colon contents were collected. The inflammation of nasal mucosal tissue was assessed via HE staining. 16S rDNA sequencing was used to detect and analyze the structure and content of bacteria in colon contents. Flow cytometry was used to detect the relative proportions of Treg and Th17 cells in peripheral blood. ELISA was used to measure the levels of Th17- and Treg-related cytokines in serum. Results Compared with the Control group, the Model group showed an inflammatory response in nasal mucosal tissue, along with increased IL-17A and IL-17E levels and decreased IL-10 levels. The percentage of Th17 cells in peripheral blood increased, while the percentage of Treg cells decreased. Beneficial bacteria in the intestine were decreased, while pathogenic bacteria were increased. Compared with the Model group, the STZQ group showed lower serum IL-17A and IL-17E levels and higher IL-10 levels. The percentage of Th17 in peripheral blood decreased, while the percentage of Treg increased. There was an increase in beneficial bacteria in the intestine and a decrease in pathogenic bacteria. The changes in the microbiota were correlated with IL-17A, IL-17E, and IL-10 levels. Conclusion She-Ti-Zhi-Qiu decoction can ameliorate the inflammation of AR by regulating gut microbiota and Th17/Treg immune balance.
Animals ; T-Lymphocytes, Regulatory/drug effects* ; Th17 Cells/drug effects* ; Gastrointestinal Microbiome/drug effects* ; Rats, Sprague-Dawley ; Drugs, Chinese Herbal/therapeutic use* ; Female ; Rhinitis, Allergic/microbiology* ; Rats ; Cytokines

Objective To explore the application of "kite" flap in soft tissue defect of eyelid and periocular skin.Methods A triangular ("kite") flap was designed in close proximity to the defect after eyelid and periocular skin lesions.Under orbicular muscle or fascia,the flap with muscle or fascia was removed.Since 2011,we have selected 50 patients aged 16-70 years.Results All incisions were primary healing,no recurrence of the lesion and all flaps survived,Follow up for 1 to 5 years showed that the scar of the incision was not obvious,no tissue organ was displaced,and the form of the eyelid and eyebrow on both sides was naturally smooth and symmetrical.Conclusions For the soft tissue defect of the eyelid and periocular skin,not suitable for direct suture,the "kite" flap can be used for the repair,which is simple and easy,with ideal results.

Objective To investigate the therapeutic effect of double leaf flap in repairing nasal alar soft tissue defect.Methods In 25 patients with basal cell carcinoma of nasal wing skin,the defect diameter of nasal wing soft tissue between 0.5 cm and 2.0 cm was removed and the defect was repaired with double flap immediately.Results The patients in this group were all successful in surgery.The incisions were all first-stage healing,no necrosis of the skin flap,1 to 3 years of follow-up after surgery,no recurrence of cancer,and the repair of the skin flap was basically the same as the color and texture of the surrounding tissue.The scars were not obvious.The lateral nasal wing physiological radians were naturally smooth.Both sides were symmetrical and met the aesthetic standards of the nose.Conclusions With the advantages of simple design and simple operation,high survival rate and beautiful shape of nasal wing after repair,the double-leaf flap can be widely used in clinic.