1.Progress of T helper cell 17,regulatory T cell,podocyte injury and primary glomerular disease
International Journal of Pediatrics 2016;43(1):66-69,70
In recent years,T helper cell 17(Th17),regulatory T cell(Treg) and podocyte injury attrac-ted widespread attention in the pathogenesis of primary glomerular disease. Th17 cells have the function of re-cruiting neutrophils and macrophages to the infected tissue through the secretion of cytokines such as IL-17. Treg cells have immune function, mediated immune tolerance, protecting the body against inflammatory injury. The imbalance of Th17 cells increase and Treg cells decrease could play an important role in the pathogenesis and progression of primary glomerular disease. As the important part of the glomerular filtration barrier,podocyte be-comes the focus in recent years. Study on relationship among Th17,Treg,podocyte injury and primary glomeru-lar disease will provide more theoretical basis for the prevention and treatment of primary glomerular disease.
2.Growth characteristics of umbilical cord-derived versus embryonic liver-derived mesenchymal stem cells
Weiwei BI ; Lirong HE ; Lili NIAN
Chinese Journal of Tissue Engineering Research 2014;(32):5166-5172
BACKGROUND:Different sources of stem cells have different molecular characteristics and growth characteristics;therefore, there are some differences in therapeutic mechanisms and effects. OBJECTIVE:To compare mesenchymal stem cells growth characteristics form two sources. METHODS:Mesenchymal stem cells from the umbilical cord and the embryonic liver were isolated and cultured. Passage 5 cells were used to observe the cellmorphology, calculate the doubling time of cellpopulation-doubling time, identify surface markers and determine the differentiation capacity. Mesenchymal stem cells from the umbilical cord were subcultured to passages 10 and 15, and cellcurves were drawn and population doubling time was calculated. RESULTS AND CONCLUSION:Mesenchymal stem cells from these two sources in logarithmic phase were fusiform and grew spiral y with osteogenic, adipogenic, and chondrogenic capacities. The growth curves of cells were both S-shaped. At passage 5, the doubling time was (34.37±0.31) hours for embryonic liver-derived mesenchymal stem cells and (35.63±0.38) hours for umbilical cord-derived mesenchymal stem cells, and there was no significant difference (P>0.05). However, the population doubling time of passages 10 and 15 umbilical cord-derived mesenchymal stem cells was (52.6±0.53) and (53.27±0.92) hours, respectively, which was significantly difference from that of passage 5 cells (P<0.05). The cellmorphology and growth curve from two sources are basical y the same. Embryonic liver-derived stem cells are smal er and proliferate faster than umbilical cord-derived mesenchymal stem cells, but no statistical difference is found between the two types.
3.Ontogeny of drug metabolism enzymes and epigentic regulation
Hang HE ; Quancheng KAN ; Lirong ZHANG
Chinese Pharmacological Bulletin 2017;33(2):167-170,171
Great changes in drug metabolizing enzyme (DME) expression occur in the fetus and child during development. Individual hepatic DME ontogeny can be categorized into one of three groups based on developmental trajectories.Some enzymes such as CYP3A7,are expressed at highest level in the fetus dur-ing the first trimester and either remain elevated or slightly de-crease during gestation,but are silenced or reduced to relatively low levels within one to two years after birth.SULT1 A1 is an ex-ample of the second group of DME.These enzymes are ex-pressed at relatively constant levels throughout gestation and into adulthood.CYP3A4 belongs to the third DME group .These en-zymes are expressed at negligible or low levels in the fetus.Sig-nificant increases in enzyme levels are exhibited within the first one to two years after birth.The epigenetic regulation refers to genomic modifications that do not involve changes in DNA se-quence and include DNA methylation,histone modifications, and non-coding RNAs.The epigenetic regulation mechanisms are responsible for the developmental expression of DME genes dur-ing liver maturation.This review will provide a summary of DME developmental expression profiles and reveal epigenetic mecha-nisms underlying variable drug metabolism and drug response. Thus,knowledge regarding DME ontogeny has permitted im-proved capability to predict drug disposition in pediatric pa-tients,which is crucial for improving drug dosing leading to opti-mal safety and efficacy in children.
5.Influence of heme oxygenase-1/endogenous carbon monoxide system on atherosclerotic process in rabbit
Danan LIU ; Zuoyun HE ; Lirong WU
Medical Journal of Chinese People's Liberation Army 1983;0(02):-
Objective To study the changes in heme oxygenase-1/endogenous carbon monoxide system in rabbit aorta with atherosclerosis induced by high cholesterol diet, and the influence of the system on the atherosclerotic process. Methods Rabbits were given 1.5% cholesterol diet (Ch group, n=8) or 1.5% cholesterol diet plus hemin(15mg?kg~ -1 ?d~ -1 , ip., Hm group, n=8) or zincprotoporphyrin IX (Znpp-IX,45?mol?kg~ -1 ?d~ -1 , ip., Zn group, n=8).After 12 weeks,serum lipids and total bilirubin, aortic CO protduction and HO activity were assessed, the area of atherosclerotic plaques in aorta were measured by oil-red-O staining and image analysis software. Results Compared with control group (C group, n=8), aortic HO activity and CO production were higher significantly, and the aortic plaques area was (54.00?4.16)% in Ch group. Znpp-IX injection markedly decreased aortic HO-1 activity and CO production, the aortic plaques area was (61.13?3.50)%. Compared with Ch group, hemin injection increased markedly not only serum total bilirubin concentration, but also aortic HO activity and CO production, and the aortic plaques area was (17.88?3.01)%. Conclusion The HO-1/CO system may play a protective role against atherosclerosis. Enhancement of HO-1 activity is possibly the basic function of HO-1/CO system in prevention of atherosclerosis.
6.Diagnosis and treatment of allergic bronchopulmonary aspergillosis: clinical features of eight cases and literature reviews
Guihua WANG ; Song LIU ; Lirong HE ; Zhengyi HE ; Haoyan WANG
Chinese Journal of General Practitioners 2009;8(4):241-244
Objective To study clinical features, diagnosis and treatment of allergic bronchopulmonary aspergillosis (ABPA) . Methods Clinical data and auxiliary examinations of eight patients with ABPA were analyzed, as well as relevant literatures were reviewed. Results All patients had symptom of cough, expectoration, out of breath and wheezing tale, seven of them with history of asthma, and all of them had increased peripheral blood eosinophil (EOS) count, serum levels of total immunoglobulin E (IgE) and lgE specific for Aspergillus fumigatus and showed positive prick test specific for Aspergillas fumigatus. Six of them showed infiltration shadows and six of them showed bronchiectasis in their chest CT images. Lung function test indicated obstructive ventilation dysfunction or mixed ventilation dysfunction with reversible airflow obstruction after administration of bronchodilator. Conclusions Diagnosis for ABPA should be made by integration of clinical features, increased blood eosinophil count and serum level of IgE, and result of prick test specific for Aspergillus fumigatus. Corticosteroid is a mainstay therapy for ABPA, ancillary with anti-fungi therapy. Patients with ABPA should be followed-up to prevent its recurrence.
7.Economic benefits of developing and sharing medical information resources in big data environment
He HUANG ; Huanqun SU ; Lian HE ; Lirong ZHENG
Chinese Journal of Medical Library and Information Science 2016;25(8):70-72
Developing and sharing medical information resources in big data environment play an important role in joint developing and sharing medical information resources in our country.The economic benefits of developing and sharing medical information resources in big data environment were thus elaborated in aspects of their products and service according to their beneficial theory for their rational location.
8.Quality and efficiency of fetal heart screening by spatio-temporal image correlation with tomographic ultrasound imaging compared with two-dimensional ultrasonography
Ruike PAN ; Lixue ZHOU ; Xin ZHANG ; Lirong HE
Chinese Journal of Perinatal Medicine 2011;14(10):577-582
Objective To explore how to elevate the efficiency of fetal heart screening.Methods Volume data of 83 normal fetuses ranging between 20 to 24 gestational weeks were acquired by spatiotemporal image correlation (STIC) with the apical four-chamber view(4CV).Tomographic ultrasound imaging (TUI) was then used to present the different views of fetal hearts,and were compared with those aquired by 2-dimensional (2D) ultrasound.Results All the 83 volume datasets were obtained by STIC.(1) Qualification ratio of short axis view of the aorta (SAV) by TUI was higher than that of 2D (94.0% vs 84.3%,x2 =5.57,P=0.042).While,in the other 8 planes,the qualification ration by STIC were higher than that of 2D,however no significant difference was found (P>0.05).(2) In 4CV,5-chamber view (5CV),left ventricular outflow tract view (LVOT),right ventricular outflow tract view (RVOT) and SAV,STIC showed excellent consistency with 2D (Kappa =0.79,0.90,0.92,0.93 and 0.77,respectively).And in long axis view of the aortic arch (LAV-AoA),long axis view of the duct (LAV-DA),superior and inferior vena cava (SVC+IVC) and short axis view of the ventricle (SVV),STIC showed good agreement with 2D (Kappa=0.72,0.67,0.74 and 0.70,respectively).(3) Image collecting time of T2D,TSTIC,TTUI and TSTIC+TUI were (5.80±1.58) min,(0.85±0.18) min,(2.57±1.32) min and (3.29±1.13) min,respectively.There were significant difference between TSTIC and T2D(t=3.500,P=0.000) and between TSTIC+TUI and TTUI (t=2.877,P=0.001).Conclusion The technique of STIC-TUI used in this study can display the standard planes of fetal heart conveniently,clearly and in shorter time.It is helpful for improving the quality and efficiency of fetal heart screening.
9.Clinical observation on dezocine composed with ropivacaine for epidural analgesia after abdominal hysterectomy
Zengting LU ; Aiting LIN ; Lirong ZENG ; Qitao HE
Clinical Medicine of China 2013;(7):751-754
Objective To investigate the postoperative epidural analgesia and adverse reactions in total abdominal hysterectomy with dezocine and ropivacaine.Methods Sixty elective transabdominal hysterectomy patients with American Society of Anesthesiologists (ASA) Ⅰ-Ⅱ grade were randomly divided into two groups,dezocine group and morphine group,there were 30 cases in each group.Dezocine group:dezocine (6 mg) + 0.75% ropivacaine hydrochloride(20 ml) + saline dilution,diluted to 100 ml.Morphine group:morphine(6 mg) + 0.75 % hydrochloride ropivacaine (20 ml) + saline dilution,diluted to 100 ml,for postoperative epidural analgesia.Both two groups were adopted in LCP mode with a loading dose:dezocine group,dezocine(2 mg) +saline dilution,diluted to 5 ml; morphine group,morphine (2 mg) + saline dilution,diluted to 5 ml.Continuous infusion of 2 ml/h,a bolus dose of 2 ml,and a lockout interval of 15 min.The analgesia duration was 48 h.Visual analogue scales (VAS) evaluation was employed to assess the analgesic effect,and the postoperative epidural analgesia adverse reaction were also recorded.Results The analgesic effect was both satisfied in the two groups.Comparing the VAS scores postoperative at different time point (4 h,8 h,12 h,24 h,36 h,48 h),dezocine group(2.7 ± 0.4,2.5 ± 0.6,2.2 ± 0.5,1.5 ± 0.5,1.3 ± 0.5,1.1 ± 0.3) were slightly lower than morphine group (2.8 ± 0.5,2.6 ± 0.7,2.3 ± 0.6,1.6 ± 0.7,1.5 ± 0.6,1.2 ± 0.4),but the difference was not significant (within the group:F =2414.96,P < 0.01 ; between the group:F =0.63,P > 0.05 ; interactive:F =2.42,P >0.05).Comparison of VAS scores at different time points after operation in two groups,the difference was not statistically significant; And within the group,there was not,the difference was not significant at the time point at 12,24,36,48 h compare to postoperative at 4 h (P < 0.01).the Adverse reaction like nausea and vomiting,skin itching occur rate is lower in dezocine group (3.3 % (1/30),0 (0/30)) than that of in morphine group(26.7% (8/30),20.0% (6/30)),and there were signficant differences between the two groups (P =0.026,0.024,P < 0.05).Conclusion Dezocine composed with ropivacaine is safe and effective,and with few adverse reactions in total abdominal hysterectomy postoperative epidural analgesia,it is worth of widely use in clinical.
10.Clinical significance of counting follicles in diagnosis of polycystic ovary syndrome by the three-dimensional ultrasound imaging with sonography based automated volume calculation method
Lirong HE ; Lixue ZHOU ; Ruike PAN ; Xin ZHANG
Chinese Journal of Obstetrics and Gynecology 2011;46(5):350-354
Objective To investigate clinical significance of counting follicles classification by three-dimensional imaging with sonography based automated volume calculation(SonoAVC)in the diagnosis of polycystic ovary syndrome(PCOS).Methods Eighty cases with PCOS were counted classified follicles and determined ovarian volume by three-dimensional(3D)imaging with SonoAVC method matched with 60 infertile women with fallopian tube or male factors as control.Main clinical.biological and other ultrasonographic markers were assessed during the early follicular phase,and the relationship between the follicle number range per ovary or the volume per ovary and the major hormonal features of PCOS was studied.Results Three-dimensional ultrasound imaging with SonoAVC methed provides a new path for objective quantitative assessment of follicle count.ovarian volume,total follicle numbers.The volume of (11 ±8)ml,total numbers of 27 ±14 follicle and number of22 ±19 follicle with diameter of≥2-<6 mm in PCOS patients were significantly higher than(6 ±4)ml in ovarian volume.6 ±4 in total follicles and 2 ±3 in follicle with diameter of≥2-<6 mm in controls(P<0.05).while follicles were similar for the≥6-≤9mm range(P>0.05).Total follicle numbers and follicles≥2-<6 mm had significantly positive relationships with ovarian volume ( r= 0. 600, 0. 618, P<0. 01 ) and level of testosterones ( r= 0. 364,0. 291, P<0.05), follicles ≥2-<6 mm also had significantly positive relationships with total follicle number (r=0. 916,P<0. 01 ). The follicles within the ≥6 - ≤9 mm range was significantly and negatively related to ovarian volume and total follicle numbers ( r = - 0. 618, - 0. 263, all P = 0. 001 ), but no significantly related to the major hormonal features of PCOS. The ovarian volume was significantly positively related with luteinizing hormone ( LH)/follicle stimulating hormone (FSH) ratio ( r= 0. 282, P = 0. 010)but negatively related to FSH level (r = - 0. 226, P = 0. 042). Conclusions Ovarian volume, total follicle numbers and follicles ≥2 - <6 mm in PCOS patients were significantly higher than those in controls. The larger ovarian volume might produce more total follicle and follicles ≥ 2 - <6 mm. The higher level of testosterone might produce more total follicle probably, which mainly result in more follicles ≥2 -<6 mm.These morphologically ultrasonographic characteristics could reflect pathophysiological changes in PCOS. Obviously, it has important clinical significance to count follicles in patients with PCOS by the threedimensional ultrasound imaging with SonoAVC method.