ObjectiveTo explore the factors related to infections of intravenous catheter as well as preventive measures.MethodsIn 598 cases of patients with central venous catheterization,indwelling time,location,bacteriology were monitored among 130 patients with suspected venous catheter infection.ResultsIndwelling catheter lasts for 3 days at least,70 days at most.For 77 out of toual cases,microbiology culture of catheter tip and blood culture were positive,accounting for 12.88％.Detected pathogens were 151 in total.Gram-negative bacteria were 48(31.79％),gram-positive bacteria were 78(50.65％),fungal were 25 (16.56％).Among these,the most common pathogens were Staphylococcus epidermidis,Staphylococcus aureus,Candida and Acinetobacter,most of the bacteria had a high degree of drug resistance.The choice of position:infection rate of subclavian vein and basilic vein was lower than that of the superclavian vein and internal jugular vein,infection rate of catheterization time ≤ 13d was lower than that of ≥ 14d.ConclusionsThe choice of subclavian vein and basilic vein,standardization of puncture technical,strict catheter care,shortening of catheterization time and improvement of the general condition of patients can reduce catheter-reiated infections.The rational use of antibiotics is a critical measure to reduce vascular catheter infections of patients.

BACKGROUND:An intensivestudy on ovarian cancer A2780 cels contributes to modify treatment strategies targeting ovarian cancer stem cels and enhance survival rate of ovarian cancer patients.
OBJECTIVE:To investigate the effects of umbilical cord blood mesenchymal stem cels (UC-MSCs) transplantation combined with paclitaxel on proliferation, apoptosis, and invasion of A2780 human ovarian cancer celsin vitro.
METHODS:A2780 human ovarian cancer cels were assigned into three groups: blank control group (routine cel culture),UC-MSCs group (addition of suspension containing 1×106UC-MSCs) and combined treatment group (combined addition of suspension containing 1×106UC-MSCs and 250μL of 1μmol/L paclitaxel solution). CD133+antigen expression, proliferation, apoptosis, and invasion were determined by immunofluorescent staining, flow cytometry, TUNEL staining, and Transwel chamber invasion assay, respectively.
RESULTS AND CONCLUSON:CD133+antigen expression intheUC-MSCs group and combined treatment group was significantly decreased compared withtheblank control group (P< 0.05). Proliferation and invasion abilities were significantly decreased, while apoptosis cel number was significantly increased inthecombined treatment group compared with the other two groups (P< 0.05). These findings indicate that the combined treatment of UC-MSCs transplantation and paclitaxel can inhibit proliferation and invasion and promote apoptosisof A2780 human ovarian cancer cels.

BACKGROUND:Cancer related genes and pathways play a critical role in formation and development of cancer.
OBJECTIVE: To investigate the silencing epidermal fatty acid binding protein 5 (FABP-5) by siRNA interference on biological characteristics of the cervical carcinoma cel line SiHa.
METHODS:Design and synthesis of siRNA interference sequence used to transiently transfect SiHa cels was performed according to FABP-5 mRNA coding sequence. mRNA and protein expressions of FABP-5 were detected by RT-PCR and western blot assay, respectively. Meanwhile, cel cycle, proliferation, invasion and apoptosis were detected by flow cytometry, cel counting bit-8 assay, Boyden chamber assay, and TUNEL staining, respectively.
RESULTS AND CONCLUSION: FABP-5 mRNA and protein expressions, cel growth speed, cel number at S phase (cel cycle was arrested at the G0/G1 phase) and cel invasion were significantly decreased, while the cel apoptosis was significantly increased in FABP-5 siRNA group compared with the negative control and blank control groups. Our findings indicate that the specific silencing FABP-5 gene expression by siRNA interference can inhibit SiHa cervical cel growth and proliferation, but accelerate cel apoptosis. Subject headings: RNA, Smal Interfering; Fatty Acid-Binding Proteins; Uterine Cervical Neoplasms; Cel Proliferation; Apoptosis

To investigate the effects of intra-articular injection of sinomenine on interleukin-1beta (IL-1beta) content in synovial fluid and serum in rabbits with osteoarthritis (OA).

Objective: To get a general estimation of the pneumatic system in ventilators and supply technical assistance for screening and removing the faults of pneumatic component. Methods: Pneumatic system of three kinds of ventilators, Sevro-i/s, Rapheal XTC and PB840 were analyzed and the differences of the three pneumatic systems were compared. Results:Known the construct and differences of pneumatic system. Conclusion: Pneumatic system is the major component of ventilator, and the analysis of the pneumatic component is benefited for the common troubleshooting of ventilators.

Objective To observe therapeutic effects of treating stroke hemiplegia with acupuncture combined with rehabilitation training. Methods 72 patients with stroke hemiplegia were randomly recruited into a control group and a treatment group, with 36 patients in each group. The treatment group was treated with acupuncture combined with rehabilitation training and the control group was treated with acupuncture exclusively. Both scalp acupuncture and body acupuncture were used in the process of treatment. Results There was statistical significance (P<0.01) between the two groups in functional rehabilitation of limbs and ADL score (P<0.01) . Conclusion The treatment of stroke hemiplegia with acupuncture combined with rehabilitation training is more effective than that of treating with acupuncture exclusively.

Objective To investigate the insulin injection-related knowledge of the registered nurses in first-class general hospitals in Shanghai and look into its influence factors. Methods Totally 331 nurses in the hospitals were enrolled in this study. Univariate and multivariate stepwise regression were used to analyze the factors which influenced the insulin injection-related . Results The aggregate score of insulin injection-related knowledge was 54.20 ± 7.64, and the score index was 67.75%. Multiple linear regression analysis showed that such factors as professional title and nursing experience and training in the endocrinology department were the main factors of insulin injection-related knowledge (all P<0.05). Conclusion We should enhance the training to promote the injection skills so that they can assist the patients to effectively control blood sugar and improve the quality of life.

Objective:To investigate the feature of depression in patients with Parkinson's disease(PD) and the related factors.Methods:Use Hamilton Depression Scale(HAMD) to measure the level of depression of 60 patients with PD.Functional state of the patients was evaluated by Motor Dysfunction Rating Scale for Parkinson's Disease(MDRSPD).Results:29 cases(48.3%) were identified to have depression,among whom 40% were of minor or moderate degree,8.3% were severe.The main manifestations of depression were anxiety/body,retardation,hopelessness and insomnia.Scores of hopelessness,retardation,and insomnia factor were significantly higher in the rigidity and tremor combining rigidity patients than those in tremor patients.The level of depression positively correlated with the duration of disease and degree of dysfunction due to PD,and it has no relation with age,sex,and education.Conclusions:The incidence of depression was higher in patients with PD.PD patients of different clinical types had different characteristics of depression.The rigidity type is more likely to get depression.The degrees are closely related to duration of disease and degree of dysfunetion.

Objective To establish a novel canine model of severe acute cholangitis(ACST).Methods Ten dogs were randomly divided into two groups:model group and control group.ACST models were established by placing T-tube in common bile duct and ligating the distal bile duct,then injecting E.coli trough T-tube and closing the T-tube.The changes of peripheral WBC,total bilirubin,ALT,AKP,IL-1?,TNF-? and endotoxin were observed pre-operatively and at 24 h,48 h,72 h,and 7 d post-operatively.The pathological changes of bile duct were observed when the dogs were sacrificed.Results There were no significant differences in all indices between two groups pre-operatively.Serum IL-1?,TNF-?,endotoxin and total bilirubin,ALT,AKP level in model group increased gradually and were higher than control group(P

OBJECTIVE:To study the effect of ligustrazine injection(LTZ)on DNA synthesis of vascular endothelial cell.METHODS:In vitro cultured cell line of human umbilical vein endothelial cells,ECV304,were adopted to measure the ef?fects of LTZ on DNA synthesis by 3 H-TdR incorporation.RESULTS:LTZ could inhibit DNA synthesis of ECV304in a dose-dependent manner.CONCLUSION:The mechanism of anti-angiogenesis of LTS might be associated with inhibiting DNA synthesis of vascular endothelial cell.