1.Survey and analysis of community healtfa service institutions across the country
Chinese Journal of Hospital Administration 2001;0(08):-
A survey and analysis were conducted at the end of 2002 of urban community health service institutions in 31 provinces, autonomous regions and municipalities directly under the Central Government so as to find out about the basie situation of community health service institutions across the country. The result indicated that by the end of 2002, the number of cities that had conducted community health service work reached 358. The coverage of community health services in cities of the prefecture level, areas under the jurisdiction of a city and cities of the county level was respectively 92. 1%, 80. 3% and 28.0% . 12 132 community health service institutions were set up in 2002, marking an increase of 31.6% as compared with 2001. The growth of community health services was fast and yet uneven in various regions. It is suggested that more efforts be made to strengthen community health services in medium-sized and small cities and improve the management of institutions and the system for statistical analysis of in-formation.
2.Sexual psychology changes and analysis of its related factors for ovarian cancer patients after operation
Chinese Journal of Practical Nursing 2008;24(21):15-17
Objective To evaluated the sexual psychology changes and marriage quality for ovarian cancer patients after operation and supply selective psychological nursing intervention for patients and their family members.Methods Self-designed questionnaire was used to investigated ovarian cancer patients under 60 years old one month after operation to understand the sexual psychology changes and its related factors.Nursing intervention was adopted according to different conditions of patients.Results Factors that influenced the sexual psychology of patients included:age,education degree,chemotherapy process,clinical symptom,anxiety and depression status,marital status and degree of nursing intervention.Conclusions Sexual psychology Was influenced by many factors.We should adopt various kinds of nursing intervention to promote the physical and mental health of patients,facihtate the recovery of sexual function and improve their life quality.
3.Cloning of the prostate cancer related genes with mRNA differential display
Xuezhen YANG ; Liqun ZHOU ; Jian ZHOU
Chinese Journal of Urology 2001;0(08):-
Objective Cloning of some specific genes related to the prostate cancer. Methods Specimen from a patient with prostate cancer and from a normal adult were studied by the improved mRNA differential display and the differentially expressed sequence-tags were cloned,sequenced and analized.Reverse transcriptive-polymerase chain reaction(RT-PCR) was used to examine the expression level of Clusterin in prostate cancer tissues,prostate cancer cell line and normal prostate tissues. Results Significant difference was observed between the two kinds of tissues in gene expression and seven differentially expressed sequence-tags were found through analysis in GenBank.Among them,five were proved to be new gene tags,one shared 100% homology with Clusterin,which was highly expressed in the prostate cancer tissue and the other one shared 97% homology with superoxide dismutase 1 (SOD1),which was highly expressed in the normal prostate tissue.It was proved by RT-PCR that to compare with the internal marker gene ?-actin,the expression level of clusterin in prostate cancer is much higher than that of normal prostate. Conclusions Five new gene tags,clusterin and SOD1 were found to be differentially expressed between tissues of prostate cancer and normal prostate and may play important roles in carcinogenesis and development of prostate cancer.
4.Expression of dihydrodiol dehydrogenase 2 in prostate cancer and its significance
Weidong SONG ; Liqun ZHOU ; Lili LIANG
Chinese Journal of Urology 2001;0(09):-
Objective To study the expression of dihydrodiol dehydrogenase (DD) in prostate cancer and its significance. Methods With reference to expression of ?-actin gene,the expression level of a human dihydrodiol dehydrogenase isoform (DD2) mRNA was examined in prostate cancer tissues (11 cases) and normal prostate tissues (10 cases) by reverse transcriptive-polymerase chain reaction(RT-PCR). Quantitative determination of relevant band densities was performed using densitometry-scanning techniques. Results Strong expression of DD2 mRNA was detected in prostate cancer tissures with absorbance in the range of 0.550 to 1.018 (median,0.726),and low expression of DD2 mRNA in normal prostate tissues with absorbance in the range of 0.248 to 0.420 (median,0.333). The difference of the expression of DD2 mRNA between cancer and normal prostate was significant ( P
5.A clinical survey of combined use of different drugs in the treatment of chronic prostatitis(report of 153 cases)
Ran TAO ; Liqun ZHOU ; Gang WANG
Chinese Journal of Urology 2001;0(10):-
Objective To study the efficacy of consecutive drugs in the treatment of chronic prostatitis. Methods 153 cases of moderate & severes chronic prostatitis were treated with consecutive drugs with sensitive antibiotics,? 1-blocker,inhibitor of cox-2 and traditional Chinese herb,20 cases being chronic bacterial and 133 nonbacterial. They were classified and evaluated according to the CPSI & EPS examinations as analyzed by T test. Results All cases were followed up for an average of 11.8 months(8~18 months).The average CPSI score was 32.6(32.7?5.3) before treatment and 13.4(12.8?7.3) after treatment (P
6.Causal analysis of re-operation for radical cysectomy and ileal conduit in early follow-up
Ran TAO ; Liqun ZHOU ; Yunxiang XIAO
Chinese Journal of Urology 2001;0(04):-
Objective To analyze the cause of re-operation after radical cystectomy and ileal conduit in early follow-up period in order to improve the efficacy of this operation and reduce postoperative complications. Methods 12 cases from 81 cases of re-operation were studied retrospectively after radical cystectomy and ileal conduit for bladder tumor in early follow-up period.Of the 12 cases,there are 4 cases of T 4,8 cases of T 3,7 cases of G 2,5 cases of G 3.They are all transitional cell carcinoma. Results There were 4 cases of adhesive intestinal obstruction,2 necrosis of ileal conduit,2 adhesive folding of sigmoid colon into prostatic fossa,2 internal hernia and 2 avulsed incision due to infection .All the cases were re-operated nearly the first operation.After the second operation,all the cases were followed 6~12 months,average 7.7 months,the outcome was satisfied. Conclusions The ileal conduit is a kind of ideal methods for urinary diversion and its early complications should be avoided affecting the efficacy of this operation and the quality of life patients.
7.A cDNA microarray study on differentially expressed genes in prostate cancer
Liqun ZHOU ; Xuezhen YANG ; Xiao HUANG
Chinese Journal of Urology 1994;0(02):-
Objective To detect differentially expressed genes in prostate cancer by cDNA microarray. Methods Total RNA was isolated by one step protocol from prostate cancer (CaP) and from normal prostate,and Poly(A) +RNA was further purified through Oligitex mRNA Midi Kit.Then it was analysed for differentially expressed genes in CaP and normal prostate by cDNA microarray with 4 096 human genes. Results There were 341 differentially expressed genes between CaP and normal prostate,of which there were 128 down regulated and 213 up regulated ones for CaP. Among these genes,15 were the most significant with 6 down regulated and 9 up regulated. Conclusions cDNA microarray can be used effectively to find out differentially expressed genes in prostate cancer which is not regulated by any single gene.Many different kinds of genes are involved in the initiation and evolution of CaP carcinogenisis.
8.Expression and significance of clusterin in prostate neoplasm
Liqun ZHOU ; Xuezhen YANG ; Lili LIANG
Chinese Journal of Urology 2001;0(03):-
Objective To study the expression and significance of antiapoptosis facter-clusterin in prostate neoplasm. Methods Reverse transcriptive polymerase chain reaction(RT PCR) was used to examine the expression level of clusterin in prostate cancer tissues(3 cases),prostate cancer cell line PC3M and normal prostate tissues(3 cases). Results Compared with the internal marker gene ? actin , the expression level of clusterin in prostate cancer is much higher than that of normal prostate. Conclusions The high expression level of clusterin in prostate cancer indicates that clusterin may play an important role in the biological characteristics of prostate cancer through the antiapoptosis pathway.
9.Diagnosis and management of renal angiomyolipoma(report of 72 cases)
Zhisong HE ; Xiaochun ZHANG ; Liqun ZHOU
Chinese Journal of Urology 2001;0(03):-
Objective The diagnosis and treatment of renal angiomyolipoma were studied. Methods A total of 72 cases of renal angiomyolipoma encountered and treated from Jan. 1983 to Dec. 1999 were reviewed. Results Of the 72 cases, 13 were misdiagnosed preoperatively.The positive diagnosis rate of ultrasonography was 73.6%(53/72) and that of CT 81.9%(59/72).10 cases( 13.9 %) had been misdiagnosed preoperatively with the combined use of ultrasonography and CT.Tumors smaller than 4 cm had a significantly higher misdiagnosis rate( P
10.Variation of serum PSA with age in benign prostatic hyperplasia patients
Liqun ZHOU ; Weimin CHEN ; Yanqun NA
Chinese Journal of Urology 2000;0(05):-
Objective To establish age specific reference ranges of serum total prostatic specific antigen (T PSA) and to investigate the relationship between T PSA,free PSA (F PSA) and ratio of F PSA/T PSA(F/T) with age in benign prostatic hyperplasia (BPH) patients. Methods 913 consecutive cases were studied retrospectively.In all of them with the age ranged from 30 to 89, serum T PSA and F PSA levels were assayed.All the patients were suspected to be BPH or PCa,103 being diagnosed as PCa on biopsy under B ultrasound and the other 810 being BPH. Results The incidence rate of PCa was 11.3% in this group.Serum T PSA and F PSA levels were positively correlated with the age ( r=0.22,P