Objective To assess the impacts of management of psychological health on metabolic indictors in community residents.Methods A total of 300 health check-up adults from one single community were randomly assigned to the control group (receiving routine health management,n =150) and the study group (receiving psychological health management,n =150).Blood pressure (BP),fasting plasma glucose (FPG),total cholesterol (TC),triglyceride (TG),low-density lipoprotein cholesterol (LDL-C) and life style were evaluated and compared at baseline and 1-year follow-up.Results No difference of clinical data was found between the two groups at baseline.After 1-year intervention,current sigarette smoking,BP,FPG,TC,TG and LDL-C of the study group were significantly decreased when compared with the control group (x2 =7.54,t =6.43,5.38,5.89,6.15,4.36,6.69,5.47 ; all P ＜ 0.05).Compliance to and satisfaction with health management were significantly improved in the study group (x2 =9.13,12.35,11.96,10.98,9.95 ; all P ＜ 0.05).Conclusion Management of psychological health may be effective in improving healthy life style,patients' satisfaction and cardiovascular metabolism in community residents.

Objective To observe the application of alkaline cleaning agent in disinfection and supply room cleaning of medical devices, to evaluate the cleaning quality. Methods The 800 contaminated medical instruments collected from February 2016 to February 2017 in municipal maternal and child health hospital were divided into control group (February 2016 to August 2016) and study group (September 2016 to March 2017 ) according to different time periods,with 400 pieces in each group.In the reference group, the instrument was washed with multienzyme detergent, and the study group was cleaned with alkaline detergent. The cleaning of 2 groups of instruments was observed and compared. Results The total excellent and good rate of instrument cleaning in the study group was 97.25％ (389/400), in the reference group was 89.50％(358/400), the difference between the two groups was statistically significant (P<0.05); the cleaning safety, practicality, economy, comprehensive score difference of the study group was significantly better than the reference group (P<0.05). Conclusion The effect of alkaline cleaning agent used in disinfection supply room cleaning medical instruments is remarkable, and the equipment residue and microorganism can be effectively eliminated, and the utility model has the advantages of high safety and low economic cost, and has the important practical value.

Objective To investigate the clinical significance of the changes of hemodynamics and electrolyte during orthotopc liver transplantation.Methods We studied 9 patients undergoing elective orthotopic liver transplantation without venovenous bypass,drew out arterial blood for monitoring arterial blood gas tensions,Na~+,K~+,Ca~(2+) and observed changes in hemodynamics and electrolyte.Results In most patients,arterial pressure significantly decreased at the prophase of the anhepatic phase,and tended to normal range in other phases.The concentration of calcium maintained a low-level during the whole surgery.During the prophases of the anhepatic phase and neo-hepatic phase,there was a light hyperkalemia,and the concentration of natrium slightly increased since 30 minutes after the anhepatic phase and neohepatic phase,but insensibly exceeded normal range.With resuming of the stability of hemodynamics,these changes had followed.Conclusion We shoud give our attention to correct the occurrence of the hypocalcemia during the forepart of the anhepatic phase and neo hepatic phase,and care for the hyperkalemia 5 minutes after the block of liver blood purveyance and revascularization of liver.The lock of body capability shoud be recruit duly according to the lose of blood and hemacytometer changes.In order to prevent serious academia and maintain the stability of hemodynamics and electrolyte,vasoconstrictor shoud be used carefully during the anhepatic phase.

Objective To investigate the effects of health management on risk factors of cardiovascular diseases in community residents.Methods A total of 208 adults from one single community who visited our health check-up center were randomly assigned to the control group(receiving routine consultation,n =104)and health management group(receiving health management intervention,n =104).At 1-year follow-up,fasting blood glucose(FBG),lipid profiles,and blood pressure(BP)were measured and risk factors of cardiovascular were surveyed.t or x2 test was used for data analysis.Results No difference of clinical data was found between the two groups at baseline.Follow-up results indicated that absolute risk of cardiovascular diseases,number of risk factors,health satisfaction,sick leave,daily vegetables intake and physical exercise,and BP were significantly improved in the health management group.Ten-year absolute risk of cardiovascular diseases(0.8 ± 0.4 vs 1.3 ± 0.5),number of risk factors(172 vs 283),and sick leave per year(118 d vs 264 d)were significantly decreased in the health management group when compared to the control group(all P ＜ 0.05).Furthermore,health satisfaction(83.7％(87/104)vs 52.9％(55/104)),and daily vegetables intake(520.5 g vs 348.3 g)and physical exercise(1.2 h vs 0.3h)were significantly improved in the health management group(all P ＜ 0.05).Conclusion Health management may be effective in decreasing risk factors of cardiovascular diseases in community residents.

Objective To observe the effect of maternal deprivation(MD)on learning and memory ability and hippocampal pathology and nestin expression in rats with hypoxic -ischemic brain damage (HIBD).Methods The models of HIBD SD male rats were established by the method of Rice,and were randomly divided into 2 groups:MD group and control group.In addition,the sham -operation group(sham group)models were established.The MD group rats were separated from their mother 3 hours per day from the second day after modeling to the 21 st postnatal day.After 28 postnatal days,Morris water maze was used to evaluate the learning and memory ability of the rat models.HE stai-ning was employed to observe the hippocampal pathological change in the rats.Then,the expression of nestin in the hip-pocampus was measured by the method of immunohistochemistry.Results Their body mass changes showed that quali-ty of sham group was higher than that of the control and the MD groups,and quality was improved in the control group, compared with the MD group,and the differences were statistically significant(q =9.860 8,3.880 7,5.980 1 ,all P <0.05).The water maze scores of the MD group in place navigation test and spatial probe test were much lower than that of the control group and the sham group,and the scores of the sham group were higher than that of the control group,the differences were statistically significant(all P <0.05 ).The findings of HE stain showed that the pathology in the right -sided hippocampus of the sham group was normal and neurons were well -arranged,and that of control group was minimally abnormal,and the neurons were almost arranged orderly and remained normal.While,the pathomorpholo-gy of the MD group was obviously abnormal,the neurons were arranged disorderly,many of the neurons lost.According to the immunohistochemical findings,the number of nestin -positive cells in right -sided hippocampus of the MD group was significantly less than that of the control group,and the number of nestin -positive cells of the sham group was less than that of the MD group,which showed significant differences among the groups(all P <0.05).Conclusions MD aggravated injury to learning and memory ability of neonatal rats with HIBD,and decrease the number of nestin -posi-tive cells of MD markedly,which is not good for the recovery of brain injury.

Objective To observe the effect of early enriched environmental intervention on synaptic ultrastructure and the synaptophysin expression (p38) in hippocampal CA1 area after hypoxia-ischemic brain damage(HIBD) in neonatal rats. Methods The rat model of HIBD was made by the method of Rice in 20 Sprague-Dawley rats, and divided randomly into 2 groups: the intervention group, non-intervention group. Another 10 rats underwent sham-operation as control. From the second day after HIBD, the rats of the intervention group were kept in an enriched environment for 20 d, the rats of non-intervention group and sham-operation group were raised in standard enviroment instead. The hippocampal ultrastructure was observed with electron microscope and the p38 expression was detected immunohistochemically in all rats. Results The ultrastructure of hippocampus CA1 pyramidal neuron in the non-intervention group were distorted as compared with that in the intervention group and control group. The corrected optical densities (COD) of immunoreactive products of the hippocampal p38 were significantly decreased in non-intervention group as compared with intervention group and control group (P0.05). Conclusion Early enriched environment intervention could improve the synaptic reconstruction and reduce the ultrastructural damage induced by hypoxic-ischemia.

Objective To observe the effects of different environment stimulation on the nestin expression in hippocampus and the ability of learning and memory of rats after hypoxic-ischemic brain damage (HIBD). Methods Rat HIBD models were established by the method of Rice in 45 SD rats, then randomly divided into three groups: standard environment stimulation group (SE), enriched environment stimulation group (EE), and impoverished environment stimulation group (IE). Another 15 rats only underwent sham-operation. Different environment intervention that was designed according to Puurunen and Bourgeon’s literatures was applied to the rats on day 2 after HIBD operation. On day 28, Morris water maze was used to evaluate the ability of learning and memory. Then the nestin expression in the hippocampus was measured by immunohistochemistry. Results The ability of learning and memory of IE group reduced and was much lower than that of sham-operation group, SE group and EE group, that of SE group was lower than that of sham-operation group and EE group, but no significant difference between sham-operation group and EE group. Immunohistochemical analysis showed that nestin expression in the hippocampus of EE group significantly increased as compared with that of other groups and that of SE group was stronger than that of sham-operation group and IE group. Conclusion The EE stimulation could increase the nestin expression in the hippocampus of neonatal rats with HIBD, enhance neuranagenesis, and improve the ability of learning and memory, while the IE stimulation could decrease the nestin expression, inhibit neuranagenesis, and impair the ability of learning and memory.

Objective To investigate the expression of E-cadherin in prostate cancer and its relationship with PSA. Methods E-cadherin expression of 56 prostate cancer samples were studied by immunohistochemical stain and its expression level was analyzed with respect to T-PSA, F-PSA and F/T ratio. Results 24 patients (43%) were normal and 32 patients (57%) were aberrant in E-cadherin expression. E-cadherin was related significantly to the grade and stage of cancer and the changes of F/T ratio.There were no significant relationship between E-cadherin expression and T-PSA or F-PSA. Conclusions E-cadherin expression may act as a marker to the malignant degree and the prognosis of the prostate cancer.

Objective To observe the effects of early enriched environment intervention on the expression of growth-associated protein-43(GAP-43) in the hippocampus of rats with hypoxic-ischemic brain injury (HIBI). Methods After the establishment of HIBI model in SD rats by the method of Rice, the animals were divided randomly into 2 groups: the intervention group and non-intervention group. The sham-operation rats were used as control group. Enriched environment intervention had been administrated to the rats of intervention group since the 2nd day after HIBI. On the 3rd, 7th, 14th, 21st and 28th day, immunohistochemistry and RT-PCR were used to measure the expressions of GAP-43 protein and mRNA in the hippocampus of rats. Results The expression of GAP-43 protein and mRNA in the rat hippocampus were increased in the non-intervention group than in the control group(P

Objective To observe the effects of environmental stimulation at different stages of development on postnatal rats with hypoxia-ischemic brain injury (HIBI). Methods HIBI postnatal SD rats models were established by dissecting and ligating the left common carotid artery, and after 2 h of recovery, exposing to 8% oxygen-92% nitrogen gas mixture for 2 h. The HIBI rats were randomly divided into early intervention groups, late intervention group, non-intervention group. Intervention group were kept in enriched environment before or after weaning for 20 d. Non-intervention group and control group were kept in a standard environment. Space learning and memory were tested with Morris water maze. Results The ability of space learning and memory in early intervention group was better than that in late intervention group (P