OBJECTIVE: To explore the material foundation of kidney-yang-deficiency syndrome. METHODS: Two kinds of rat models of deficiency of kidney-yang were induced by adenine intragastric administration (model I) and hydrocortisone intramuscular injection (model II). One hundred rats were randomly divided into normal control group, model I group, low-dose model II group, medium-dose model II group and high-dose model II group. After model establishment, contents of serum creatinine (SCr), blood urea nitrogen (BUN), and urine creatinine (UCr) were detected by automatic biochemistry analyzer; freezing point method was used for 24-hour urinary osmotic pressure (Uosm) testing and kaolinite method was used to detect the content of urinary 17-hydroxycorticosteroid (17-OHCS). Expression of aquaporin-1 in renal tissue was observed by using real time reverse transcription polymerase chain reaction. RESULTS: The rats of model groups had the characteristics of kidney-yang deficiency syndrome. The contents of SCr and BUN were significantly higher in model I group than in normal control group and three model II groups (P<0.05), and UCr and Uosm were significantly lower (P<0.05). The level of urinary 17-OHCS and expression of aquaporin-1 in renal tissue were decreased in the model I group (P<0.05) as compared with the normal control group. Compared with the normal control group, the content of SCr was increased and urinary 17-OHCS was decreased in the medium-dose model II group, but the expression of aquaporin-1 was increased in three model II groups with a dose-dependent manner. CONCLUSION: Aquaporin-1 may be one of the material foundations of kidney-yang-deficiency syndrome.

A total of 184 teeth obtained from 102 cases(27 males and 75 females aged 17-64 years)were selected from Department of Tooth and Dental Pulp.DalJan Stomatology Hospital from June to Septembet 2007.All the teeth with deep caries which resulted from caries showed no pulp.Ninety-two teeth obtained from 54 cases were regarded as Vitrebond group which were used by light-cured glass to cover pulp;while,the other 92 teeth obtained from 48 cases were regarded as dycal group which were cured by dycal calcium hydroxide.Finally,3MZ250 resin was used in this study.A half-year following up showed that the filling shed and loose were observed in the Vitrebond group(n=1)and the dycal group(n=3)(P>0.05).There were significant differenoe in color between Vitrebond group(n=1)and dycal group(n=8)(P<0.05),suggesting 3M Vitrebond light-cured glass Was superior to dycal calcium hydroxide in decreasing dental pulp stimulation and improving dental appearance.

The prognosis of elderly (>60 years) patients with diffuse large B-cell lymphoma (DLBCL) is significantly worse compared with young patients, and there is currently no standard treatment. Elderly patients with DLBCL are highly heterogeneous, a stratification before treatment can help achieve precise medicine and improve outcome of them. R-CHOP (rituximab, cyclophosphamide, vincristine, doxorubicin and prednisolone) is still the recommended treatment for fit elderly DLBCL patients; and for unfit or very old patients, chemotherapy of reduced dose or palliative treatments should be considered. Choices for relapsed or refractory patients are limited, and novel compounds or therapies that are well tolerated may have a good application prospect.

OBJECTIVE: To study the effects of high-protein diet (HPD) and high-grade proteinuria in aggravating the progression of chronic renal failure (CRF) in rats. METHODS: CRF with high-grade proteinuria was induced by supplying HPD in five sixth nephrectomy rats, and the changes of serum creatinine (Scr), blood urea nitrogen (BUN), endothelin-1 (ET-1), thromboxane B(2) (TXB(2)) and 6-keto-prostaglandin F(l alpha) (6-Keto-PGF(l alpha)) were observed. At the same time, the content of malondialdehyde (MDA), and the activities of glutathione peroxidase (GSH-Px) and superoxide dismutase(SOD)were measured in blood and renal tissue of the rats. RESULT: HPD and high-grade proteinuria could accelerate the damage of kidney through increasing the levels of ET-1 and TXB(2), reducing the level of 6-Keto-PGF(l alpha), and attenuating the activities of SOD and GSH-Px. CONCLUSION: HPD can accelerate the damage of kidney through inducing the high-grade proteinuria in five sixth nephrectomy rats, influencing the expression of kidney vasoactive substance, and reducing the anti-oxidation.

ObjectiveCurrently,adsorption materials applied to remove low density lipoprotein (LDL) still remain defects.HB-H-8 resin has not been put in use in removing LDL.This study focused on primary evaluation of adsorption capability of HB-H-8 resin.MethodsHB-H-8 resin was used in the adsorption of LDL of patient that was higher than the normal range of blood sample in dynamic test and static test in vitro.ResultsThe time of adsorption saturation was two hours,and the optimum temperature was 37℃.The results of adsorption in vitro showed that the average adsorption rates of LDL and high density lipoprotein (HDL) were 63.2％ and 1.9％,respectively.ConclusionHB-H-8 resin has LDL higher adsorption capability,thus made it to be a potential blood purification material for the removal of LDL.

Objective:To observe effects of Fuzheng Huoxue Recipe on blood growth hormone (GH) and insulin growth factor(IGF-1) in chronic renal failure(CRF) rats.Methods:Platt method was adopted to make 5/6 kidney excision CRF model rat,and the rats were divided into model group,Huangqi group,Enalapril group,Danshen group,Fuzheng Huoxue Recipe group and normal control group according to the blood creatinine level after modeling.Effects of Fuzheng Huoxue Recipe on GH and IGF-1 levels in CRF rats with large quantity of urinal protein were investigated.Results:GH and IGF-1 levels in the model control group were significantly higher than that in normal control group,and after treatment of Fuzheng Huoxue Recipe,blood GH and IGF-1 levels were significantly decreased.Conclusion:Fuzheng Huoxue Recipe can decrease blood GH and IGF-1 levels in CRF rats,and can delay advances of CRF.

ObjectiveTo investigate the effects of comprehensive geriatric assessment (CGA) intervention on depression and anxiety in patients with benign prostatic hyperplasia (BPH).Methods90 patients with BPH (aged 65 years and over) from June 2009 to June 2010 were randomly assigned to control(n= 45) and CGA (n = 45) group.Control group received conventional therapy,while CGA group was given CGA and consultation therapy as well as conventional therapy.The scores of international were assayed and compared between two groups before and 6 months after treatment.ResultsThe scores of international prostate symptom score (IPSS),quality of life scale (QOLS),self-rating depression scale (SDS) and self-rating anxiety scale (SAS) in control (t= 11.0,5.84,7.08and 9.68,respectively) and CGA (t= 14.0,10.4,9.16 and 6.1,respectively)group 6 months after treatment reduced as compared with those before treatmen (all P ＜ 0.01).After 6 months of treatment,the scores of IPSS,QOLS,SDS and SAS in CGA group were lower than in control group (t= 4.25,5.55,3.45 and 2.88,respectively,all P＜0.01).ConclusionsCGA intervention can improve the quality of life in BPH patients and alleviate their depression and anxiety.

Objective To determine the relevant factors of under staging and under grading in patients undergoing radical prostatectomy and to define important predictors of under staging in this population. Methods The clinical T stages and biopsy Gleason scores were compared with the pathological T stages and prostatectomy Gleason scores in 55 patients. Results Among the 55 cases,50 were preoperatively classified as clinical stages T 1～T 2,of whom 21(42%) were postoperatively classified as pathological stages T 3～T 4.Of the 26 cases who preoperatively had the Gleason scores of 2 to 6,11(42%) had postoperatively pathological grades of 7 to 10.Logistic regression analysis revealed that PSA( P =0.0159)at diagnosis and the percentage of positive biopsies of prostate ≥50%( P =0.0013) were significant predictors for preoperative clinical staging lower than postoperative pathological staging. Conclusions The results demonstrate that patients who were classified as clinical stages T 1～T 2 with PSA≥20 ng/ml and percentage of positive biopsies≥50% may have high possibility of under staging.

Objective To investigate the expression and significance of T-bet in experimental autoimmune uveoretinitis (EAU). Methods EAU was induced in 24 Lewis rats by immunization with retinal S-antigen (50 ?g) and complete Freund′s adjuvant, and another 4 rats were the healthy control. The rats with EAU were executed 7, 12, 15, 21 days after immunization, respectively. Immunohistochemical single and double staining were performed using monoclonal antibodies of T-bet or CD4 on the ocular wholemounts and the consecutive sections of the eye and spleen from both 24 immunized Lewis rats and 4 normal controls. The positive cells were counted under the optic microscope and the data were analyzed by SPSS 11.0 statistic software. Results A few T-bet positive cells were observed in the normal ocular tissues and spleen. The expressions of T-bet in the iris, retina, and spleen increased 7 days after immunization, reached the peak at the 15th day, and decreased at the 21st day, which were higher than those in the control. Double staining on the consecutive sections revealed that most of the T-bet positive cells were positive for CD4 monoclonal antibody. Conclusion T-bet may affect the occurrence of EAU by activating Th1 cells.

The study is to investigate the relationship between dyslipidemia and impaired glucose tolerance (IGT). Ninety elderly nondiabetic patients (60 -84 years old) were under long-term follow-up in outpatient department. Blood lipid measurement and oral glucose tolerance test (OGTr) were performed,the two-hour glucose levels (2 hPBG) between normal and abnormal blood lipid groups were compared. We found that patients with hypertriglyceridemia had a significantly higher 2 hPBG than patients with normal blood triglyceride(P＜0. 01 ). But there were no differences in 2 hPBG levels between other dyslipidemias and normal lipid patients. OGTT screening in elderly patients with hypertriglyceridemia can be helpful in early detection and prevention of diabetes.