1.Effect of Mogroside extracts on blood glucose,blood lipid and antioxidation of hyperglycemic mice induced by Alloxan
Chinese Pharmacological Bulletin 1987;0(02):-
Aim To study antidiabetic effect and mechanism of Mogroside extracts(MGC) on hyperglycemic mice induced by Alloxan.Methods Alloxan-induced diabetic mice were fed with MGC at dose of 50,100?300,500 mg?kg~(-1) bw respectively for 30 d.The blood glucose levels,the blood lipid levels and antioxidation levels were assayed after the last fedding.Results ①MGC at dose of 100?300?500 mg?kg~(-1) bw significantly lowered the fasting blood glucose and the hypoglycemic effect of MGC at dose of 100 mg?kg~(-1) bw was the best ②MGC decreased the content of TC,TG and enhanced the content of HDL-C of alloxan-induced diabetic mice,so it could restore the blood lipid levels of diabetic mice(P0.01).Conclusion Mogroside extracts(MGC) have obvious glucose-lowering effect on hyperglycemic mice,its mechanism may be related with improving antioxidation level and restoring the blood lipid levels of hyperglycemic mice.
2.Signal mechanism of endothelin-1-mediated activation of airway fibroblasts induced by injured airway epithelial cells
Xingwu CHEN ; Liqin ZHANG ; Zhengui SUN
Chinese Journal of Pathophysiology 2009;25(12):2403-2407
AIM: To explore the effects of p38 mitogen-activated protein kinases (MAPK) and phosphoinositide 3-kinases (PI3K)/Akt on interleukin (IL)-6, the endothelin (ET)-1-mediated process of airway fibroblast activation induced by injured human bronchial epithelial cells (HBE). METHODS: Human primary cultured airway fibroblasts were co-cultured with HBE pre-treated with or without poly-L-arginine (PLA). The procedure was also performed in the presence or absence of p38 MAPK selective inhibitor SB203580, PI3K selective inhibitor LY294002 or ETA receptor blocker BQ123, respectively. Immunostaining, Western blotting or ELISA were used for detecting α-smooth muscle actin (α-SMA) expression, the activities of p38 MAPK and Akt in fibroblasts or IL-6 levels in supernatants of fibroblasts. In addition, fibroblasts were mixed with soluble collagen and cultured with HBE treated as the same mentioned above, the gel contraction was measured by serial area measurements. RESULTS: ET-1 and IL-6 levels [(13.69±1.36) ng/L, (56.7±10.7) ng/L] in the supernatants of fibroblasts cultured with injured HBE were significantly higher than those in the supernatants of fibroblasts cultured with HBE [(3.79±0.64) ng/L, (15.5±3.2) ng/L]. BQ123, SB203580 or LY294002 decreased IL-6 levels [(27.2±3.1) ng/L, (31.5±3.6) ng/L, (41.3±3.2) ng/L] differently in the supernatants of fibroblasts induced by injured HBE. Activation of p38 MAPK preceded Akt in fibroblasts cultured with injured HBE. BQ123 reduced the phosphorylation levels of p38 MAPK and Akt. SB203580 concentration-dependently attenuated Akt phosphorylation, while LY294002 had little effect on p38 MAPK phosphorylation. Fibroblasts expressed more α-SMA after cultured with injured HBE and showed significant increase in the gel contraction compared to fibroblasts cultured with HBE [percentage of gel contraction: (61.2±2.7)% vs (15.4±7.3)%], all these effects were diminished or inhibited by BQ123, SB203580 or LY294002. Furthermore, the effects of BQ123 and SB203580 on decreased gel contraction were stronger than the effect of LY294002. CONCLUSION: ET-1 exerts a key role in the airway fibroblasts activation induced by injured HBE through activating p38 MAPK, PI3K/Akt signaling and promoting IL-6 expression.
3.The influence of lanthanum chloride on the proliferation of human gingival fibro blasts
Xiaoping WANG ; Liqin WANG ; Jun ZHANG
Journal of Practical Stomatology 2001;0(03):-
Objective:To investigate the influence of lant ha num chloride on the proliferation of human gingival fibroblasts(HGFs).Me thods:HGFs were cultured in vitro and MTT assay was used to analyse the influence of lanthanum chloride at 10 -6,10 -5,5?10 -5,1 0 -4,10 -3 and 10 -2 mol/L respectively on the proliferation o f HGFs. Results:Lanthanum chloride at 1?10 -2 mol/L inhib ited the proliferation of HGFs,however, it did not at 10 -6~10 -3 mo l/L. Conclusion:Lanthanum chloride at a higher concentration(1 ?10 -2 mol/L)may inhibit HGF proliferation.
4.Application study of the CHA2 DS2-VASc score in the prognosis assessment of an initial cardioembolic stroke with atrial fi-brillation
Liqin ZHANG ; Feng YE ; Pinfang REN
Journal of Chinese Physician 2014;(6):763-765
Objective To investigate prognostic value of the CHA 2 DS2-VASc score [ congestive heart failure , hypertension , age≥75y (doubled), diabetes mellitus, stroke (doubled)-vascular disease, age 65~74 and sex category (female)] in an initial car-dioembolic stroke with atrial fibrillation .Methods A total of 346 initial cardioembolic stroke patients with atrial fibrillation was en-rolled.The clinical feature, the National Institutes of Health Stroke Scale (NIHSS) score on admission, and clinical outcome [modi-fied Rankin Scale (mRS) score after 90 days] were obtained and evaluated retrospectively according to the CHA 2DS2-VASc score. Results The rate of female, smoking, hypertension, congestive heart failure, anticoagulation and the level of age, NIHSS score, and CHA2 DS2-VASc score were significantly different between two prognostic groups ( P <0.05 ) .A positive correlation was observed be-tween CHA2DS2-VASc score and NIHSS score on admission (ρ=0.324, P <0.01), CHA2DS2-VASc score and mRS score (ρ=0.228, P <0.01).Logistic regression analysis showed that age , smoking, anticoagulation, the CHA2DS2-VASc score, and the NIH-SS score on admission were related to clinical outcome independently .Conclusions The CHA2 DS2-VASc score may be useful for not only evaluation of stroke risk but also prediction of clinical outcomes after stroke .
5.The Clinical Value of Carcinoembryonic Antigen, Cytokeratin 19 Fragment and Neuron Specific Enolase Levels in Lung Cancer
Qingfu DAI ; Jinzhen ZHANG ; Liqin WEN
Chinese Journal of Prevention and Control of Chronic Diseases 2006;0(01):-
0.05). Conclusion To adept the combined test of CEA CYFRA21-1 and NSE in lung flushing fluid is more sensitivity and more valuable application in clinic.
6.Situation and countermeasures of the educational inspection for colleges and universities
Liqin FU ; Jicheng LI ; Xiaoming ZHANG
Chinese Journal of Medical Education Research 2006;0(11):-
This study analyzes the reason and situation of the educational inspection for colleges and universities.The author suggests the five points to solve these problems and provide countermeasures for improving the educational inspection.
7.Effect of dexmedetomidine conscious sedation on laryngeal mask airway general anesthesia on Parkinson patients with cerebral deep electric stimulus implantation
Wenyi ZHANG ; Chi ZHANG ; Liqin LANG ; Mingqing PENG
The Journal of Clinical Anesthesiology 2017;33(5):455-458
Objective To observe the anesthesia related effect of dexmedetomidine conscious sedation on Laryngeal mask airway general anesthesia in Parkinson patients with deep brain electric stimulus implantation.Methods Forty patients (72 males, 8 females, aged 18-75 years, ASA physical status Ⅰ or Ⅱ) of Parkinson disease with selective operated bilateral subthalamic nucleus deep brain electric stimulus implantation were selected and randomly divided into dexmedetomidine group (group D) and contrast group (group C), 20 cases in each group.The operation divided into two stages: in the first stage, the electrode was put under the local anesthesia, CT examination proof of electrode placement satisfaction was the end of the first stage.The second stage needed to bury the pulse generator under of the chest skin under general anesthesia.For group D, dexmedetomidine 0.5 μg/kg was pumped venously within 15 min after entering the operating room, the speed of 0.1-0.3 μg·kg-1·h-1 was kept subsequently until the end of the first period.For group C, the same dosage of normal saline was pumped.The times of micro-electrode interference, tremor relief were recorded.Ramsay scores of two groups of patients were recorded at the entry of operating room (T0), partial infiltration anesthesia (T1), unilateral electrode implantation (T2), contralateral electrode implantation (T3), the end of the first period (T4);the time needed to vanish consciousness, implant laryngeal mask airway, recover the spontaneous breathing and extubation were recorded;the propofol, remifentanil dosage from the beginning of general anesthesia induction to the end of the surgery were recorded.Results The micro-electrode was intervened and tremble was relieved in both two groups.Compared with group C, the ramsay scores of T1-T4 were obviously increased in group D (P<0.05), the time needed to vanish consciousness, implant laryngeal mask airway, recover the autonomous respiration, extubation were significantly shortened in group D (P<0.05), the dosage of propofol, remifentanil from the beginning of the general anesthesia starts to the end of the surgery were significantly reduced in group D (P<0.05).Conclusion Dexmedetomidine provided the excellent sedation to the first period of Parkinson patients with deep brain electric stimulus implantation;it can shorten the time of the anesthesia induction and awakening time, as well as decreases propofol and remifentanil dosage.
8.The relationship between pulse pressure and urine microalbumin in patients with essential hypertension
Tao LIN ; Shulan ZHANG ; Jingtao LI ; Liqin DUAN
Chinese Journal of Postgraduates of Medicine 2014;37(31):24-26
Objective To observe the influence of pulse pressure on renal injury in patients with essential hypertension (EH).Methods Two hundred and twenty-nine patients with mild-to-moderate EH completed 24 h ambulatory blood pressure monitoring.The patients were divided into 2 groups according to 24 h mean pulse pressure (MPP):EH 1 (24 h MPP ≥ 60 mmHg,1 mmHg =0.133 kPa) group (128 cases) and EH2 (24 h MPP < 60 mmHg) group (101 cases).According to 24 h urine microalbumin and biochemical determination of renal function,the influence of pulse pressure on kidney function was observed and compared.Results The 24 h mean systolic blood pressure (MSBP) and 24 h MPP in EH1 group were significantly higher than those in EH2 group [(156.20 ±6.11) mmHg vs.(143.67 ±5.46) mmHg,(69.09 ±5.87) mmHg vs.(51.15 ±7.15) mmHg] (P <0.01),the 24 h mean diastolic blood pressure (MDBP) in EH2 group was significantly higher than that in EH1 group [(92.52 ±5.93) mmHg vs.(87.11 ± 5.52) mmHg](P< 0.01).The 24 h urine microalbumin in EH1 group was significantly higher than that in EH2 group [(104.80 ± 24.25) mg vs.(91.29 ± 24.03) mg] (P < 0.01).There was positive correlation between 24 h MPP,24 h MSBP and 24 h urine microalbumin (r =0.259,0.201,P < 0.01),and there was negative correlation between 24 h MDBP and 24 h urine microalbumin (r =-0.180,P< 0.01).Conclusion The 24 h MPP is closely related with the renal injury which is caused by high blood pressure in patients with EH.
9.Research on the Conformance Test of Health Information Standard in Canada
Yazi LI ; Liqin XIE ; Guolei LI ; Aiyun ZHANG
Journal of Medical Informatics 2015;(10):8-13
〔Abstract〕 Based on the retrieval sources of the official website of Inforway in Canada , Tongfang Knowledge Network and Internet re-sources, by investigating the conformance test of health informatization and health standards conducted by Inforway in Canada , the paper makes a comprehensive analysis of the conformance test of health information standards in Canada from 4 aspects, namely the certification subject, certification object , certification standard , certification process .It aims to provide references for the conformance test of health information standards in China .
10.Expression and purification of GPS2 and its antibody preparation
Liqin JIANG ; Xuemin LI ; Lingrong LIU ; Qingqing XIONG ; Qiqing ZHANG
International Journal of Biomedical Engineering 2012;35(3):173-176
ObjectiveThe aim was to construct the recombinant plasmid of pET-28a-G-protein pathway suppressor 2 (GPS2) GPS2,express GPS2 protein in E.coli,and obtain specific polyclonal antiserum of GPS2.MethodsGPS2 gene was obtained and the amplified fragment was then cloned into E.coli expression vector pET-28a to construct recombinant plasmid.The recombinant plasmid was transformed into E.coli expression strain BL21(DE3).IPTG induces the expression protein GPS2 protein,and the induction conditions were optimized.The induced product was purified by Ni2+ affinity chromatography,and the purified product was dialyzed with buffer for refolding.The purified protein can be used as antigen,injected to immunize male New Zealand white rabbit to get polyclonal antiserum.The titer and specificity of the rabbit antiserum were detected by ELISA and Western Blotting.ResultsThe E.coli expression vector pET-28a-GPS2 was constructed successfully and the recombinant protein was efficiently expressed and purified.The purified protein was used to immunize male New Zealand white rabbit to get polyclonal antiserum and the ELISA and Western Blot results showed that the high titer of specific polyclonal antiserum.ConclusionGSP2 could be highly expressed in E.coli.Antiserum of GPS2 protein can be obtained by the purified recombinant to analyze its function.