1.Effects of combined irbesartan/hydrochlorothiazide on morning blood pressure surge and left ventricular mass in essential hypertension
Wenwei YUE ; Xin WANG ; Liqin WANG ; Lianhua BI ; Ruyi JIA
Chinese Journal of Postgraduates of Medicine 2009;32(19):25-27
Objective To investigate the influence of combined irbesartan/hydrochlomthiazide on morning blood pressure surge(MBPS)and left ventricular mass(LVM) in essential hypertension.Methods After 4-8 weeks' run-in period,if blood pressure wagn't up to the standard,the 120 patients were given irbesartan/hydrochlorothiazide once daily from 150 mg/12.5 mg for 12 months to 300 mg/25mg for another 12 months.MBPS was conducted by arnbuhry blood pressure monitoring(ABPM)and LVM was determined by echocardiography at the end of placebo baseline and 12 months later.Results (1)After treatment the patients with MBPS(+)decreased and patients with MBPS(-) increased.(2)After treatment the extents of MBPS were reduced in MBPS(+)patients[systolic blood pressure(SBP)difference(16.1±1.8)mm Hg(1mm Hg=0.133 kPa)vs(29.4±2.8)mm Hg,diastolic blood pressure(DBP)difference(10.2±2.3)mm Hg vs(21.2±2.2)mm Hg,P<0.01]with little change in MBPS(-)patients[SBP difference(11.2±2.4)mm Hg vs(10.1±1.2)mm Hg,DBP difference(5.9±1.9)mm Hg vs(6.8±3.2)mm Hg]compared with before treatment.(3)LVM indexes of all patients were significantly reduced.Conclusion Irbesartan/hydrochlorothiazide can effectively attenuate the extent of MBPS, and has advantages on reversing left ventricular hypertrophy.
2.Expression of nuclear factor ?B in the tissuses of chorion of early spontaneous abortion
Liqin WANG ; Xuewen YU ; Xiaoge ZHAO ; Yue ZHAO
Journal of Xi'an Jiaotong University(Medical Sciences) 1981;0(03):-
Objective To examine the cellular distribution and expression,activation of nuclear factor ?B(NF-?B) in the tissuses of chorion of early spontaneous abortion,and explore the relationship between early spontaneous abortion and NF-?B.Methods The NF-?B expression on the chorionic villi was analyzed by S-P immunohistochemistry.The nucleoplasmic ratio of NF-?B was detected by laser scanning confocal microscope.Results The expression intensity of NF-?B in the syncytiotrophoblasts was significantly weaker in women with early spontaneous abortion than in the normal early pregnancy(P
3.Effects of repeated intrathecally KIF17 antisense oligodeoxynucleotide on the expression of mLin10 and NR2B in spinal cord in a mouse model of bone cancer pain
Ming LIU ; Yue LIU ; Bailing HOU ; Linyu SHI ; Liqin JUAN ; Xiaoping GU ; Zhengliang MA
Chinese Journal of Behavioral Medicine and Brain Science 2014;23(8):724-727
Objective To investigate the effects of repeated intrathecally kinesin superfamily protein 17 (KIF17) antisense oligodeoxynucleotide (ODN) on the expression of mLin10 and NR2B in spinal cord in a mouse model of bone cancer pain.Methods Fifty-six male C3H/HeJ mice,aged 4 ~ 6 weeks,weighting 20 ~ 25 g,were randomly divided into two groups:sham operation group (group S,n=20) and bone cancer pain group (group T,n=36).20μl α-minimal essence medium (α-MEM) which containing 2× 105 NCTC2472 osteosarcoma cells was injected into the intramedullary space of the right femur in group T.In group S,no cancer cell was instead.The number of spontaneous flinches (NSF) and the paw withdrawal mechanical threshold (PWMT) were measured at the day before (base) and the days 4,7,10 and 14 after inoculation.According to the corresponding time points,twenty-four mice were sacrificed for determination the expression of KIF17,mLin10 and NR2B using Western blot.Then,the mice of group T were randomly divided into three groups (n=8,T1,T2,T3,group).In group S and group T1,Saline 5 μl was injected intrathecally.KIF17 sense ODN and antisense ODN,5 μg/5μl were respectively injected in group T2 and T3 for 6 consecutive days.Pain behaviors were assessed at the days 2-6 after the first injection.And determinated the KIF17,mLin10 and NR2B expression,again.Results Compared with group S,the NSF was increased and the PWMT was decreased at the days 7,10 and 14 after inoculation in group T (P<0.05).Compared with the base ((0.65±0.15),(1.06±0.06),(1.01±0.14)),the expression of KIF17,mLin10 and NR2B (14d:(1.13 ±0.06),(2.17 ± 0.37),(1.85 ± 0.32)) were increased at the days 7,10 and 14 after inoculation in group T(P<0.05).During the course of the injection,compared with group T1 and T2,the NSF was decreased and the PWMT was increased significantly in the group T3(P<0.05),the expression of KIF17,mLin10 and NR2B((0.88±0.08),(0.96±0.11),(1.03±0.08)) were reduced in group T3 (P<0.05).Conclusion Intrathecal KIF 17 antisense ODN in the mice of bone cancer pain improves the pain behaviors,and inhibits the up-regulated of KIF17,mLin10 and NR2B during the course of the injection.
4.System and intranasal immunization of group B streptococcal C5a peptide
Guanhua XUE ; Shentao LI ; Yonghong YANG ; Lihua YU ; Aihua WANG ; Guan WANG ; Liqin YUE ; Guorong YIN ; Xuzhuang SHEN
Chinese Journal of Microbiology and Immunology 2008;28(12):1137-1142
Objective To study the system of streptococcal C5a peptidase (ScpB) and the specif-ic antibody levels in serum, lung, vagina and recta after subcutaneous and intranasal immunization with dif-ferent doses of C5a peptide. Methods Recombinant protein C5a peptide was expressed in E. coli strain BL21 and purified by affinity chromatography. The expressed product was identified by SDS-PAGE and pep-tide mass fingerprinting (PMF). BALB/c mice were subcutaneously and intranasally injected with different doses of ScpB. Antibody titer was tested by ELISA. Opsonophagocytosis assay was used to test the function of antibody. Results ScpB protein was successfully expressed and purified. The probability based mouse score of ScpB was 175 by PMF analysis. ELISA data showed that both subcutaneous and intranasal immtmi-zation could elicit significantly higher levels of IgG in immunized mice serum than that of control group (P <0.01), 30 μg group waa better than 5 μg and 10 μg group. Intranasal immunization could elicit higher lev-els of IgA in lung, vagina and rectum (P <0.001) while system immunization could not. Opsenophagocyto-sis tests indicated that anti-serum of ScpB had opsenophagocytic activity than that of control (P < 0. 05).Conclusion The results demonstrated that intranasal immunization with ScpB could induce significantly higher levels of lgG and IgA, and its anti-serum had better opsenic activity.
5.Unilateral congenital microphthalmia in newborn: a case report
Yanan FU ; Zhihua ZHANG ; Qi CAO ; Jin TIAN ; Liqin YUE
Chinese Journal of Perinatal Medicine 2019;22(5):350-352
Here,we reported a case of term infant with unilateral congenital microphthalmia (CM).Physical examination conducted on 2 d after birth showed that the left eye was barely open and the eye socket was deeper than the right.Meanwhile,the cornea of the left eye was not completely exposed and the light reflex could not be elicited.Ophthalmology consultation,ultrasound and CT scan were conducted,and CM was finally diagnosed since the infant was found to have a small eyeball and shortened axial length,accompanying by pathological changes in the lens,vitreous body and retina.This case suggested that detailed physical examination should be carried out,especially for the eyes,including orbit and eyelid,presence or absence of secretion or concealed eyeball,together with medical imaging techniques technology,to ensure an early detection and diagnosis of CM in neonates.
6. Threshold value of lactic acid and buffer excess in umbilical cord arterial blood gas during neonatal asphyxia
Shaodong HUA ; Liqin YUE ; Liming CHENG ; Shumei WANG ; Zhenhui ZHOU ; Qiuping LI ; Zhichun FENG
Chinese Pediatric Emergency Medicine 2019;26(12):901-906
Objective:
To study the threshold values of lactic acid (Lac)and buffuer excess (BE) when neonatal umbilical arterial blood gas is at pH <7.20.
Methods:
A total of 2 518 pregnant women admitted in Beijing New Century Women and Children′s Hospital from January 2017 to February 2019 were enrolled.The general conditions in perinatal period and labor methods were counted.Umbilical cord arterial blood was immediately collected before the first cry of newborn and was detected on the Abbott I-STAT automatic blood gas analyzer from the United States, and then the umbilical arterial blood gas was measured.We analyzed the perinatal factors affecting umbilical arterial blood gas.We studied the threshold values of Lac and BE for neonal asphyxia by receiver operating characteristic curve.
Results:
(1)Maternal anemia, Streptococcus agalactiae infection, precipitate labour, placenta abnormalities had no significant impact on neonatal umbilical artery blood gas.The Lac value in blood gas was higher in the group of premature rupture of fetal membranes and fetal intrauterine distress.The value of BE was lower in the group with premature rupture of fetal membranes and fetal distress (
7.Design, synthesis, and biological evaluation of multiple targeting antimalarials.
Yiqing YANG ; Tongke TANG ; Xiaolu LI ; Thomas MICHEL ; Liqin LING ; Zhenghui HUANG ; Maruthi MULAKA ; Yue WU ; Hongying GAO ; Liguo WANG ; Jing ZHOU ; Brigitte MEUNIER ; Hangjun KE ; Lubin JIANG ; Yu RAO
Acta Pharmaceutica Sinica B 2021;11(9):2900-2913
Malaria still threatens global health seriously today. While the current discoveries of antimalarials are almost totally focused on single mode-of-action inhibitors, multi-targeting inhibitors are highly desired to overcome the increasingly serious drug resistance. Here, we performed a structure-based drug design on mitochondrial respiratory chain of
8.Clinical practice guideline for body composition assessment based on upper abdominal magnetic resonance images annotated using artificial intelligence.
Han LV ; Mengyi LI ; Zhenchang WANG ; Dawei YANG ; Hui XU ; Juan LI ; Yang LIU ; Di CAO ; Yawen LIU ; Xinru WU ; He JIN ; Peng ZHANG ; Liqin ZHAO ; Rixing BAI ; Yunlong YUE ; Bin LI ; Nengwei ZHANG ; Mingzhu ZOU ; Jinghai SONG ; Weibin YU ; Pin ZHANG ; Weijun TANG ; Qiyuan YAO ; Liheng LIU ; Hui YANG ; Zhenghan YANG ; Zhongtao ZHANG
Chinese Medical Journal 2022;135(6):631-633