Objective To explore the effects of modified radical surgery combined with chemo-therapy on postoperative complications and quality of life in breast cancer patients.Methods The clinical data of 175 patients with early breast cancer were retrospective analyzed.According to the different ways of surgical treatment,patients were divided into the control group(n =100)and the study group(n =75).In the study group,patients received modified radical mastectomy,and preoperative and postoperative chemo-therapy as adjuvant therapy.In the control group,patients received traditional standard radical surgery.The follow-up ranged from 1 to 3 years.Recurrence,mortality,postoperative complications,and quality of life were compared between the two groups.Results There were no significant differences in local recurrence (6.7% vs 6%)and axillary lymph node recurrence (2.7% vs 3%),distant metastasis (10.7% vs 11%),and 5-year survival rate(92% vs 91%)between the study group and control group(P >0.05).In the study group,the incidence of postoperative complications was significantly lower(6.7% vs 42%), while its score on quality of life significantly improved(P <0.05).Conclusion For early breast cancer, modified radical mastectomy combined with chemotherapy has similar effects as the standard radical mas-tectomy.It has obvious advantages in reducing complications and improving the quality of life,which is worthy of being spread clinically.

OBJECTIVE:To investigate medical staff’s awareness and knowledge level on the rational use and management of antibacterials in our hospital.METHODS:A questionnaire survey was conducted on the spot among medical staff in our hospital,and the results of survey were recorded in Excel electronic form and subjected to data analysis and processing.RESU-LTS & CONCLUSION:The medical staff had a good mastering about the general knowledge about antibacterials(such as definitions,classifications and so on).However,their answers to the questions such as reasonable antibacterial drug use,bacterial resistance,safety and expertise were relatively concentrated(tended to tick the same answer).Therefore,it is advisable to develop different forms of training through different ways among hospital medical staff to enhance their knowledge level on antibacterials and strengthen their awareness of correct and rational use of antibacterials meanwhile setting related measures and management norms.

Objective To investigate the relationship between the zinc and copper concentration and the clinical outcomes of in vitro fertilization and embryo transfer(ivf-et).Methods The clinical data and zinc and copper concentration of serum on the days with human Chorionic gonadotropin(hCG) injection as well as that in the follicular fluid on the the day of oocyte retrieval were retrospectively analyzed in 43 cases with IVF-ET cycles.Results Application of pearson correlation analysis showed that copper concentration in follicular fluid of the 43 cases was positively correlated with the numbers of retrieved oocytes,fertilized oocytes and cleaved embryos( r were 0.392,0.327 and 0.321,respectively,P were 0.0094,0.0325 and 0.0361 respectively ) Between the pregnant group and non-pregnant group,the zinc and copper concentration in follicular fluid was not significantly different ( P ＞ 0.05 ) ; the serum estradiol ( E2 ) on the day of hCG administration was of significantly different(t =2.177,P =0.0353 );the serum zinc concentration on the day of hCG administration was of significantly different ( t =2.153,P =0.0372).Conclusion Trace elements zinc and copper might play an important role in the process of IVF-ET.

Objective:To analyze and evaluate population health informatization development of China. Meth-ods:Through this investigation, 8 578 copies of a questionnaire designed for all levels of health administration and parts of primary hospitals were collected. These collected data, were analyzed to evaluate the population health infor-matization development of China through the evaluation index system established for population health informatization development and comprehensive evaluation model. Results:The results of this empirical study indicate that the popu-lation &Health Informatization overall has rapid development , but still has regional differences, and total score of the top 10 provinces are Zhejiang, Shanghai, Jiangsu, Liaoning, Beijing, Chongqing, Tianjin, Hunan, Henan and Gan-su. A statistical difference (p<0. 05) exists in regional development on population & health informatization of Chi-na, therefore serval provinces have obvious weakness or advantage in the construction of informatization. Discussion& suggestions:Government of China should pay attention to evaluation during the construction of informatization;pro-mote a balanced and sustainable development by strengthening financial support and optimizing the input pattern;up-grade regional population health informatization overall according to the information resources as the core;and develop the key priorities of population health informatization depending on practical situation and developing trend.

AIM: To investigate the effect of roxithromycin on the NF-?B activity,the airway inflammation and bronchial hyperresponsiveness of asthma rats.METHODS: Thirty male adult Sprague-Dawley rats were randomly divided into the control group(Group C,n=10),asthma group(Group A,n=10) and roxithromycin group(Group R,n=10).The asthma model was induced by ovalbumin and Al(OH)3.The airway reactivity was detected and then rats in each group were sacrificed after 24 hours for the last provocation.The concentrations of IL-4,IL-5 and IFN-? in bronchoalveolar lavage fluid(BALF) were measured by ELISA,and the activity of NF-?B protein in bronchial epithelium was detected by immunohistochemistry.RESULTS: Compared with Group C,the airway reactivity in Group A was increased(P

Objective To study the expression of PINCH and vascular endothelial growth factor C (VEGF-C) in cervical squamous cell carcinoma.Methods We detected the expression of PINCH and VEGF-C by immunohistochemistry SP in 58 cervical squamous cell carcinoma cases and 30 normal cervical epithelial tissue and analyzed their relationship to the clinical pathological features.Results The expression of PINCH and VEGF-C in 58 cervical squamous cell carcinoma(62.1％,36/58 ;67.2％,39/58 ) were higher than that in normal cervical epithelial tissue(0,0/30).The difference was significant( x2 =31.512,12.534,P ＜ 0.001 ).The expression of PINCH protein was not significantly associated with the age,tumor size and tumor differentiation grade ( P ＞ 0.05 ),but was associated with the lymph node metastasis and clinical stage ( x2 =9.090,8.236,P ＜ 0.001 ).The expression of VEGF-C had no significant correlationship with the age and tumor size( P ＞ 0.05 ) but had a correlationship with the lymph node metastasis,tumor differentiation grade and clinical stage( x2 =10.775,13.496,5.001,P ＜ 0.05 ).The expression of VEGF-C was correlated with the expression of PINCH protein( C =0.341,P ＜ 0.01 ).Conclusion It is possible that VEGF-C and PINCH take part in the development and progress of cervical squamous cell carcinoma and play an important role in the invasion and metastasis mechanism altogether.

A glucose biosensor was fabricated from a glucose oxidasE-immobilized by chitosan and oxygen electrode. The effects of concentration of chitosan(0.3%), enzyme loading(0.8 mg), pH 7.0, phosphate buffer concentration(300 mmol/L), and temperature 25 ℃ for the response of the biosensor were investigated. The glucose biosensor has a linear response range of 0.016-1.10 mmol/L with a detection limit of 8.0 μmol/L(S/N=3). The response time was less than 60 s. The biosensor showed extremely good stability with a shelf-life of at least 3 months. The biosensor exhibited good repeatable response to a 0.25 mmol/L glucose olution with a relative standard deviation of 2.5%(n=10). The precision of fabrication of the biosensors using four different membranes was good with a RSD of 4.7%. Some common potential components in sample such as niacinamide, vitamin B6, vitamin B12, vitamin E, Ca2+, Mg2+, K+ and Zn2+ showed no interferences on the response of the glucose biosensor. The biosensor was successfully applied to determine the glucose in commercial beverage samples.

Objective To explore the distribution of inflam m atory cells and positive expression of P-se-lectin glycoprotein ligand-1 (PSG L-1) in infant brainstem tissue from hand-foot-m outh disease related fatal brainstem encephalitis. Methods Tw enty brainstem sam ples from infants suffered from brainstem en-cephalitis w ere collected as the experim ental group. Ten brainstem sam ples from infants died of non-brain diseases and injuries w ere collected as the control group. The distribution of inflam m atory cells and the expression of PSG L-1 in the tw o groups w ere exam ined by im m unohistochem ical m ethod. The characteristics of the positive cells w ere observed. Results In brainstem tissue of the experim ental group, there w ere sleeve infiltrations of inflam m atory cells around the vessels and in the glial nodule. Microglia was the m ost and following was neutrophils around the vessels and in the glial nodule. There was a significant statistical difference am ong m icroglias, neutrophils and lym phocytes (P<0.05). There was no sleeve infiltration in the control group. PSG L-1 protein was expressed w idely in inflam m atory cells in the experim ental group, especially in the inflam m atory cells around the vessels and in the glial nodule. B ut PSG L-1 positive staining could be observed significantly less in the control group com paring with the experim ental group (P<0.05). Conclusion Microglia is the m ain type of inflam m atory cells involved in the progress of the fatal disease. Moreover, PSG L-1 could participate in the pathogenesis of hand-foot-m outh disease related fatal brainstem encephalitis.

Objective To evaluate the distribution of fetal abnormal chromosome karyotype in mid-pregnancy and analyse the possible misdiagnosis risks of molecular techniques in clinical prenatal diagnosis.Methods Fetal karyotype ( fetal cell collected from amniotic fluid ) in Prenatal Diagnosis Center of Zhejiang Province between 2001 and 2010 were retrospectively analyzed on distribution according to 7 different referral indication:positive screening for trisomy 21, trisomy 18, advanced maternal age , abnormal history of pregnancies , abnormal family history , fetal structural abnormalities and others.The combination of trisomy 21, trisomy 18 and trisomy 13 ( T21/18/13 Group) and the aneuploidies of chromosome 21, 18, 13, X, Y (21/18/13/X/Y Group) were further analyzed based on the current molecular target detection range.Results There were 462 cases out of 12 481 with chromosomal abnormality (3.60%, 462/12 841), with 215 cases of high risk (detection rate 1.67%, 215/12 841) and 247 cases of low risk (detection rate 1.92%, 247/12 841).Under different indications , the detection rate on abnormal chromosome of high risk (high-risk CA) is different,“abnormal fetal ultrasound” is the highest(27.27%,24/88).Among the high-risk CA, T21/18/13 Group accounted for 72.56%(156/215), while the 21/18/13/X/Y Group accounted for 94.88%(204/215).For the 7 regular indications , the high-risk CA distribute different;Except the T21/18/13 Group and 21/18/13/X/Y Group, the rates of other abnormal chromosome karyotype in the high risk CA were 0.28%( 2/719 )-12.5%( 11/88 ) and 0.06%( 4/6 915 )-1.14%( 1/88 ) according to different indication, respectively.Conclusions The distribution of abnormal karyotype were different under different referral indication;the detection power and possible misdiagnosis risks were varied under different indication for each molecular technique.It was suggested that doctors should select suitable molecular technique according to different clinical indications and each molecular method has its own limitations .

Pathology is a subject that studies the etiology,pathogenesis,pathological changes,progression and outcome of diseases.Pathology links the basic research and clinical practice and is an important part of translational medicine.In order to cultivate qualified pathological graduates with solid pathological theories and the abilities of proposing and addressing scientific hypotheses from pathological morphology changes,we employ modularized special training to divide the pathology training courses into morphology learning module,article searching and reading module,project design module,experiment operation module and scientific presentation module.The training contents among these modules are relatively independent but closely connected,and compose a strategy that aims to improve the scientific innovation ability of pathological graduates.