Objective To study the effectiveness of Suhuangzhike Capsule on eosinophilia (Eos) and exhaled nitric oxide (FeNO) in patients with cough variant asthmathe.Methods 98 patients with cough variant asthmathe were randomly divided into the two groups,the control group (n =49 cases) and the observation group (n =49 cases).The patients in the control group were treated through the conventional treatment,while the patients in the observation group were treated through the conventional treatment plus Suhuangzhike Capsule.They were treated for four weeks.Eos and FeNO were detected before and after treatment.Results The total effective rate of the control group was 87.8％,which of observation group was 95.9％;The total effective rate of the observation group was obviously higher than that of the control group (x2 =4.86,P ＜ 0.05).The two groups after treatment the blood Eos and FeNO were significantly lowered (t =5.102,8.772,7.921,10.224,P ＜ 0.05,P ＜ 0.01).And compared with the control group after treatment,the patients in the observation group decreased more significantly (t =2.714,3.336,all P ＜ 0.05).Conclusion Suhuangzhike Capsule can decrease eosinophilia and exhaled nitric oxide in patients with cough variant asthmathe.

The total effective rate of Xiaoying Plaster applied top-ically for dissipating or reducing the size of the masswas 84.34% while that of the control group was 42.9%.Animal experiment also revealed that local appli-cation of this plaster alone can markedly reduce thethyroid mass,restoring the diffuse proliferation of thy-roid tissue to normal.The antigoiter effect is similar tothat of oral administration of thyroid tablet.

Objective To investigate the role of eotaxin (ehemotacfie factor of eosinophils) in pathogenesis of eosinophilie airway inflammation and airflow obstruction. Methods 45 eases of patients in exacerbations with bronchial asthma (A group) and 25 eases of healthy volunteers as controls (B group) have participated in lung func-tion test and sputum induefion. The concentration of eotaxin was measured with sandwich enzyme-linked immunosor-bent assay (ELISA). Results The differences of the eosinophil (EOS)/leukoeyte (Leu)% in the two groups are significant (P <0.05) ; the differences of the eotaxin in the two groups is also significant (P < 0.05) ; the EOS/Leu% and the level of eotaxin are significantly inversely correlated with the percentage of the first second forced ex-piratory volume/predicted value (FEV1%) (P < 0.001). The level of eotaxin in induced sputum is significantly positively correlated with the EOS/Leu% in A group (P<0.001). Conclusion Eotaxin poasiblely partieulatesin e-osinophilic airway inflammation and airflow obstruction in asthmatic patients by selective chemotactic activity on eosin-ophil, and eosinophil release a series of inflammation medium that induce the exacerbation of asthma.

Objective To investigate the effect of cisplatin and 5-fluorouracil on endocrine Ishikawa cells in the treatment of endometrial cancer. Methods The human endometrial cancer cell line Ishikawa were selected, at the logarithmic growth period, the cells were divided into three groups of 5-fluorouracil (5 mg/L) group, cisplatin (5 mg/L) group and cisplatin (5 mg/L) combined with fluorouracil (5 mg/L) group ( combined group).After treated with corresponding drugs treatment, the cell viability was detected by MTT, the apoptosis was detected by flow cytometry, and the Bcl-2 and p65 expressions cells were detected by Western blot.Results The inhibitory rate of combined group, cisplatin group and 5-fluorouracil group were (41.45 ± 3.13)%, (25.20 ±3.09)% and (23.19 ±4.10)% respectively, and the inhibitory rate in the combined group was significantly higher than that of the other two groups (P<0.05).The apoptosis rate of the combined group, cisplatin group and 5-group were (29.44 ±4.35)%, (5.74 ±1.12)% and (5.82 ±1.78)% respectively, and the apoptosis rate in the combined group was significantly higher than that of the other two groups (P<0.05).The Bcl-2 relative expression in the combined group, cisplatin group and 5-fluorouracil group were (0.31 ±0.11), (1.23 ±0.49) and (1.28 ±0.59), the p65 expression were (0.67 ±0.23), (1.67 ±0.56) and (1.71 ±0.71), combined group of Bcl-2 and p65 relative expressions were obviously less than that of the other two groups ( P <0.05 ) .Conclusion Cisplatin combined with 5-fluorouracil on endocrine Ishikawa cells in the treatment of endometrial cancer could promote cell apoptosis, inhibit the cell proliferation, and its mechanism may be related to the inhibition of Bcl-2 and p65 protein expressions.

It is increasingly evident that sepsis triggers a complex host reaction, which is responsible for a variety of pathophysiologic changes during the inflammatory process. Pentoxifylline (PTX) is a methyxanthine with selective anti inflammatory activity. The effects and mechanisms of PTX in human and animal models of sepsis are reviewed, for providing new information in treatment of sepsis.

Objective:To investigate the influence of lant ha num chloride on the proliferation of human gingival fibroblasts(HGFs).Me thods:HGFs were cultured in vitro and MTT assay was used to analyse the influence of lanthanum chloride at 10 -6,10 -5,5?10 -5,1 0 -4,10 -3 and 10 -2 mol/L respectively on the proliferation o f HGFs. Results:Lanthanum chloride at 1?10 -2 mol/L inhib ited the proliferation of HGFs,however, it did not at 10 -6~10 -3 mo l/L. Conclusion:Lanthanum chloride at a higher concentration(1 ?10 -2 mol/L)may inhibit HGF proliferation.

BACKGROUND: Traditional Chinese medicine (TCM) has clear efficacy and fewer side effects, because of its own pharmacological action, as well as the trace elements in it.OBJECTIVE: To determine the contents of metal elements and their total flavone in Scutellaria baicalensis Georgi (SBG).DESIGN: Repeated measurement trial.SETTING: Jilin Medical College, Affiliated Hospital of Jilin Medical College.MATERIALS: SBG was bought on the market. Rutoside sample(F 20050930, Sinopharm Group Chemical Reagent Co., Ltd.).METHODS: The experiment was carried out in the Department of Trace Element in Chinese Herbal Medicine, Jilin Medical College from April to July in 2006. SBG samples were dried and powdered then digested with nitric acid-perchloric acid wet method. And 1.000 0 g was weighed accurately, 3 portions were used for each kind of sample, placed in 25 mL coni cal flask, 10 mL concentrated HNO3 was added, then sealed for overnight.10 mL concentrated HNO3 and 5 mL HClO4 were added respectively on the next day, and slowly heated on the electric hot plate; when only about 1 mL left, 20 mL concentrated HNO3 and 5 mL HC1O4 were added for continuous digestion till the solution was colorless and clearing and almost dried. It was diluted and dissolved with 30 g/L nitric acid, then transferred to 50 mL volumetric flask, evenly mixed for measurement. Atomic absorption spectrophotometer was used to detect macroelement Na, Ca, Mg, K and trace element Fe, Mn, Cu, Zn. Alcohol extraction method was applied to extract the total flavone of SBG while UV spectrophotometry for the content of total flavone.MAIN OUTCOME MEASURES: The contents of metal elements and their total flavone.RESULTS: The contents of metal elements and total flavone in SBG were detected as follows: K (1 098.516 8±2.731 9) μg/g; Ca (6 561.552 6±78.716 7) μg/g; Na (9 530.491 2±44.176 6) μg/g; Mg (6 555.442 8±36.860 3) μg/g; Fe (512.075 0±5.135 9) μg/g; Cu (2.905 0±0.134 2) μg/g;Zn (30.081 0±0.740 0) μg/g; Mn (12.409 2±0.247 7) μg/g; Total flavone(17.496 2±2.134 4) mg/g.CONCLUSION: Metal element and their total flavone are rich in SBG.

Objective To investigate the role of central and peripheral sensitization in remifentanil-induced hyperalgesia in a rat model of inflammatory pain. Methods Twenty-one male SD rats weighing 200-300 g were used in this study. Inflammatory pain was induced by intraplantar injection of 1 % carrageenan 100 fd in the left hindpaw in all animals. The animals were then randomly divided into 3 groups ( n = 7 each): control group (group C) and two remifentanil groups (group R1 , R2) . In R1 and R2 groups remifentanil was infused iv at a rate of 10 and 30 μg-kg-1·min-2 respectively starting from 5 min before till 25 min after carrageenan injection, while in group C normal saline was infused iv instead of remifentanil. Bilateral paw withdrawal threshold to mechanical stimulation with von Frey filament (PWT) was measured before (baseline) and at 1 h, 3 h and 1-7 d after carrageenan injection. Bilateral paw withdrawal latency to noxious thermal stimuli (PWL) was measured before and at 2 h, 4 h and 1-7 d after carrageenan injection. The thickness of the plantar surface of left hindpaw was measured before and at 1 h, 4 h and 1-7 d after carrageenan injection. Results Bilateral PWT was significantly lower at day 1 after carrageenan injection in R, and R2 groups than in group C. The right PWT was significantly lower at 2 d and 4-7 d after carrageenan injection in group R2 than in group R, . There was no significant difference in PWL and thickness of the plantar surface of left hindpaw among the 3 groups. Conclusion Central sensitization is involved in developing and maintaining the remifentanil-induced hyperalgesia in a rat model of inflammatory pain, while peripheral sensitization is not.

Objective:To investigate the clinical pharmaceutical care methods and promote rational drug use. Methods:The diag-nosis and treatment process of one patient with chronic obstructive pulmonary disease were used as the examples, the pharmacists par-ticipated in selecting drugs, adjusting dosage and making individual regimen to carry out the pharmaceutical care in the whole treatment process, including observing curative effect, monitoring adverse reactions, implementing patient education,nutritional support, immu-notherapy and the effect evaluation. Results:Clinical pharmacists together with clinicians developed the treatment regimens for the pa-tient with the full implementation of pharmaceutical care, the effect was promising, and the patient recovered and discharged from hos-pital. Conclusion:Clinical pharmacists should implement pharmaceutical care for patients with chronic obstructive pulmonary disease in the whole treatment process, which can reduce drug adverse reactions and interactions effectively, and play an important role in ra-tional and safe drug use.

Objective To study the effect of using handhold magnifier on reading speed in patients with low vision and normal-sighted people. Design Prospective,control study. Participants Low vision (13 patients)and normal-sighted readers (37 persons) who use handhold magnifiers or the first time. Method Nine-point text was read by normal vision subjects without magnifier and then with two magnifiers with different magnification (+10 D,+20 D) in different eye-to-magnifier distance (10 cm,35 cm). Nine-point text was also read by low vision people with magnifiers. The big printed text under the same magnification without magnifier was also read by low vision group. Reading speed was recorded. Main Outcome Measures Reading speed (words per minute). Result For normal vision subjects,reading speed without magnifier (194.6?45.2 words/min) is faster than that with a 10D magnifier in an eye-to-magnifier distance of 10cm (159.7?44.7 words/min) (P=0.001),and distance of 35cm (162.5?46.7words/min) (P=0.002),respectively. Reading speed without magnifier is also faster than that with a 20D magnifier in an eye-to-magnifier distance of 10cm (150.3?43.3 words/min) (P