Objective To study the distribution characteristics and relationship of acetylcholinesterase (AChE) .substance P(SP), vasoactive intestinal polypeptide (VIP) and nitric oxide synthase ( NOS)-positive neurons in intestinal tract of rats. Methods The distributions of AChE,SP, VIP and NOS-positive neurons in duodenum,jejunum,ileum,colon and rectum of rats were respectively observed by using enzyme histochemical and immunohistochemical methods. Results Four types of positive neurons distributed throughout in each laminae of intestinal tract of rats,and there was accompanying and resemble distribution regularity. From duodenum,jejunum to ileum,four types of positive neurons showed gradual increase tendency in quantity, while from colon to rectum, they showed gradual decrease tendency in quantity. There were extremely abundant AChE-posi-tive neurons in each laminae of intestinal tract of rats,comparatively rich NOS and SP-positive neurons in Auerbach'myenteric plexus(AMP) ,and comparatively rich VIP-ir positive neurons in the Meissner's submucosal plexus(MSP)and mucosa. Density on the distribution of four types of positive neurons was AChE>NOS>VIP>SP. There was coexistence of four types of neurons, and there were synapse-like contacts in four types of neurons in AMP. Conclusion There were significant differences in the density on the distribution of AChE,SP, VIP and NOS-positive neurons in different segments of intestinal tract of rats,and there was close morphological relationship among four types of neurons in AMP. It was deduced that they exerted synergistic effects in regulating motility of the intestine.

Objective To provide histology base for the microsurgical repair of the free gracilis muscle transplantation after brachial plexus.Methods Totally 6 fresh male adult cadaveric inferior extremities were obtained.The gracilis muscle nerve were exposed and divided with the microdissection.Specimens were got from different segment after marked direction.All specimens was faced in 4％ formaldehyde solution and then crossing sections were cut by cryoultramicrotome.All slides were stained use the technique of Kamovsky-Roots AchE histochemical.The result of never tissue staining and the distribution of individual functional fascicular group were observed on each cross-section.According to the result of staining combined with the microdissection and the order of different branches branching off the nerve trunk,the distribution of individual functional fascicular group were observed on each cross-section.The 5 μm-thick routine waxed crossing sections were made and stained according to the myelin technique of Loyez.These histological sections were analyzed by using image analysis system.For each histological section,the number of the medullated nerve fibers and the section areas of the each nerve tracts and trunks were measured and calculated.Then the proportion of nerve tracts and connective tissue were calculated.The proportion of each connective tissue was adopting paired-samples t test.Results Under low power lens most of the gracilis muscle nerve were positive reaction,only a few sparse block-shape enzyme staining regions were shown.Under high power lens,the gracilis muscle nerve have clear outline,enzyme staining limited at neuraxis,no staining at myelin sheath and connective tissue.Quantitative analysis shows that the total myelinated fiberscilis nerve was about (1958 ± 375) radix.The branches arising from the posterior subdivision were more than that from the anterior (P =0.000).There were statistical difference between the number of the medullated nerve fibers and the section areas of the anterior and posterior subdivisions,the posterior subdivision were more than that of the anterior (P ＜ 0.05).There also had statistical difference between different section areas of the connective tissue in the gracilis nerve trunk (P ＜ 0.05),the section areas of the connective tissue of the distal were more than that of proximate.Conclusions The motor fascicles characteristic of the gracilis muscle nerve can be distinguished clearly by using AchE histochemical staining,combin with the microanatomy results,we can gain the distribution of the fascicular groups on each crossing sections.Using Loyez staining and quantitative analysis,we can ensure the number of the medullated nerve fibers.It is helpful to select the suitable donor nerve and ensure the dialyneury matching each other.

Objective To investigate the clinical characteristics and the causative mechanism of acetabular fracture and dislocation of hip complicated with sciatic nerve injuries. Methods A retrospective analysis was done for 155 cases (159 sides) of acetabular fracture, dislocation of hip, or acetabular fracture combined with hip dislocation, 35 of which were complicated with sciatic nerve injuries. The epidemiological features of acetabular fracture and hip dislocation, the incidence, and relationship between sciatic nerve injury and classifications of acetabular fracture and dislocation of hip were analyzed. Results 81.3%of the patients were male. The ages of 86.5%of the patients ranged from 20 to 50 years old. 83.9%of the cases were injured in traffic accidents. The incidence of sciatic nerve injury was 22.01%in all the patients, 17.19%in patients with acetabular fracture, 12.90%in patients with posterior wall, and 36.36%in patients with acetabular fracture combined with posterior dislocation of hip. Conclusions A road traffic accident is the major causative factor for acetabular fracture and dislocation of hip. Most victims are male. Sciatic nerve injuries largely happen in cases of acetabular posterior wall fracture and posterior column fractures combined with posterior dislocation of hip. Peroneal nerve injuries are the most common type of sciatic nerve injury.

The subunit ?_0 of guanine nucleotide- binding protein, in the areas of rat brain and spinal cord was localized by immunohistochemical methods. It was found that in the rat brain, specific ?_0-like immunoreactivity(?_0-Li) displayed regional heterogeneity, a high density of ?_0-Li revealed in neuropil, and somatic membranes as well as the neuronal processes.Most intense ?_0-Li can be seen in substantia nigra(pars reticulata), interpcduncular nucleus, habenulo-interpeduncular tract, strata oriens and radiatum of the hippocampus, and substantia gelatinosa of the spinal cord. There are also areas of moderate staining ie: the molecular layer of cerebral and cerebellar cortex, habenula, caudate-putamen complexes, the midline nuclei of thalamus and hypothalamus, periaqueductal grey, grey layers of superior colliculus, the olivo-cerebellar tract and the spinal tract of the trigeminal nerve as well. By contrast, the immunoreactvity of ?_0 in septal nuclei, globus pallidus, red nucleus, and the regions adjacent canalis centralis of the spinal cord showed much weaker. In addition, on the membranes and the processes of the neuronal cell bodies in the periaqueductal grey, substantia nigra, reticular formation, medial geniculate body and the nucleus of the trapezoid body were ?_0-Li positive.The results of AChE staining revealed that the AChE-positivc nerve terminals was coinsident with the presence of ?_0-Li in the following regions. For instance: the molecular layer of cerebral cortex, hippocampus, spinal tract of the trigminal nerve, and substantia gelatinosa of the spinal cord, where both the ?_0-Li and the AChE activity were positive. It is suggested that ?_0 subunit of Go-protein in brain might play roles in membrane signal transduction, and might have some relationship with cholinergic nerve.

1. 5 cm) sessile colorectal polyps referred for EMR. After submucosal injection of epinephrine, either en bloc or piecemeal snare polypectomy were performed. All resected specimens were retrieved for pathologic study. Follow-up colonoscopy was performed in all patients after EMR. Results All 157 polyps were removed completely. All lesions are larger than 1. 5cm, but 3 less than 1 cm on the submucosa of rectum. The largest one is 13 cm X 12 cm. No complication occurred. Histopathologic assessment of the resection specimens revealed the following: adenoma, 123; dysplasia, 80; mucosal carcinoma, 11; hyperplastic polyps, 20; rectal carcinoid, 3. Two patients who had rectal adenoma that was larger than 7cm recurrence happened at the resection site after 1 and 3 months follow-up respectively, than removed completely by hot biopsy forceps showed hyperplastic and villous adenoma on pathological study. No more residual tumor was detected for 6-12 months. Conclusion EMR with an intensive follow-up program is a safe and effective treatment for large sessile colorectal polyps and mucosal carcinoma.

AIM: To investigate the distribution in mice and pharmacokinetics in rabbits of fraction Ⅲ isolated from Naja naja atra venom. METHODS: Fraction Ⅲ was labelled with [~(125)Ⅰ] by chloramine-T method. The drug concentration in blood was determined by a radionuclide tracing kinetic methods. The distribution of [~(125)Ⅰ]-fraction Ⅲ in mice was determined based on the ratio of the relative incorporation of radioactivity in tissues to that in blood. RESULTS: In two and four hours after intravenous injection of fraction Ⅲ in mice, the organs in which the ratio of the radioactivity incorporation was bigger than 1 were liver, kidney, lung, heart and muscle, whth the maximun in kidney. After intravenous injection of fraction Ⅲ, with dosages of 75, 150 and 300 ?g/kg, respectively, the T_(1/2)?, T_(1/2)? and T_(1/2)? were 39.6-42.5 min, 16.8-17.3 h and 21.7-22.1 h, respectively. There was no significant difference between the different dosages. CONCLUSION: Fraction Ⅲ was mostly found in kidney, followed by liver and lung after intravenous administration in mice. The pharmacokinetics is in accordance with the feature of three atrioventricular modle. The AUC is in direct proportion to the dosage. It suggests that the distribution and clearance of the drug is a grade 1 linear kinetic process. [

Objective:To investigate the protective effect of lactoferrin(Lf) on lung injury in mice exposed to irradiation.Methods:C57BL/6 J mice were randomly divided into control group, 15 Gy irradiation group (IR group) and lactoferrin combined 15 Gy irradiation group (Lf+ IR group), with 5 mice in each group. The mice in the Lf+ 15 Gy group drank lactoferrin solution (10 mg/ml) from 3 days before irradiation and contained the whole experiments. Then, single chest 15 Gyirradiation was performed both in the IR and Lf+ IR groups. The body weight and other characteristics were monitored during the experiment. The mice were killed at day 14 after irradiation. The lung histopathology was observed by HE staining. Serum inflammatory cytokine such as HMGB1, TNF-α, IL-1β and IL-6 was determined by ELISA method . The expression of inflammatory related protein in lung tissue including HMGB1, TLR4, MyD88 and NF-κB were performed by immune histochemistry and Western blot method.Results:Compared with the control group, lung weight was significantly increased ( t=3.20, P<0.05), pulmonary hyperemia and inflammatory cell infiltration was observed in the IR group. Exposure also significantly increased serum level of TNF-α[(291.80±5.49) vs.(332.25±22.18)pg/ml]( t=3.07, P<0.05), up-regulated the expression of inflammatory related protein in lung tissue ( t=4.04, 4.78, 3.77, 6.14, P<0.05). Lactoferrin intervention (Lf+ IR group) significantly decreased lung weight ( t=2.18, P<0.05), alleviated histopathologic changes, decrease serum levels of HMGB1, TNF-α and IL-1β ( t=4.67, 2.97, 3.49, P<0.05). On the other hand, lactoferrin intervention decreased the positive cell number of HMGB1 and NF-κB, and down-regulated the protein expression of HMGB1, TLR4, MyD88 and NF-κB in lung tissues, with significant difference with the IR group ( t=8.06, 9.80, 3.07, 5.56, P<0.05). Conclusions:Lactoferrin plays the protective effect of radiation-induced lung injury through the downregulation of inflammatory response, such as HMGB1/TLR4/MyD88/NF-κB signaling pathway.

OBJECTIVETo discuss the diagnosis and operative technology of acetabular fracture.

METHODS41 cases of displaced acetabular fractures were enrolled. CT and 3D-CT were performed before operation to understand the classification of fractures and to determine operative regimen. All cases were fixed by reconstruction plates and screws through the best approach.

RESULTS39 cases were followed up for 1 - 4 years, with an average of 1.5 years. Curative outcomes were assessed by Matta's criteria. Clinically, 16 (53.3%) 30 fresh fractures were excellent, 11 (36.7%) good, 3 (10%) fair. The rate of excellence and goodness was 90%. In 9 cases of old fractures, 2 were excellent, 3 good, 2 fair and 2 poor. The rate of excellence and goodness was 55.6%. Complications consisted of sciatic never injury, necrosis of the femoral head, and heterotopic ossification.

CONCLUSIONSThe time of operation, degree of injury, and quality of reduction of fracture are important factors affecting the postoperative function of the hip. The key to enhance the outcome is to verdict the type of fracture, select the proper approach before operation, and precise reduction and appropriate internal fixation during operation.

Acetabulum ; injuries ; Adolescent ; Adult ; Female ; Fracture Fixation, Internal ; methods ; Fractures, Bone ; surgery ; Humans ; Male ; Middle Aged

Soluble tumor necrosis factor-related apoptosis-inducing ligand (sTRAIL) is potentially a novel anti-cancer drug due to its superior selective cytotoxicity in a wide variety of tumor cells. Zinc ion (Zn2+) insufficiency might be an important cause for weak cytotoxicity of sTRAIL prepared by gene engineering. In this paper, the sTRAIL protein is highly-expressed with insertion of the synthesized gene encoding sTRAIL into pQE30 plasmid. The polymerization and cytotoxicity in tumor cells of sTRAIL prepared in presence of different concentrations of Zinc ions were compared. It was found that the sTRAIL protein prepared in absence of Zinc ions mainly existed as monomer with weak cytotoxicity. However, in the presence of Zinc ions, sTRAIL formed homotrimer and showed strong cytotoxicity in tumor cells. These results demonstrate that Zinc ion is very important for cytotoxicity of sTRAIL. It is necessary for keeping stable activity of sTRAIL by addition of proper concentration of Zinc ion in the media.
Antineoplastic Agents ; metabolism ; pharmacology ; Breast Neoplasms ; pathology ; Cell Line, Tumor ; Female ; Humans ; Lung Neoplasms ; pathology ; Polymerization ; drug effects ; Recombinant Proteins ; metabolism ; pharmacology ; TNF-Related Apoptosis-Inducing Ligand ; genetics ; metabolism ; Zinc ; pharmacology

Objective To analyze the risk factors associated with periprosthetic femoral fracture following hemiarthroplasty (HA) for displaced femoral neck fracture in aged patients.Methods From January 2013 to June 2016,120 patients over 80 years old were treated by HA for displaced femoral neck fractures.They were 45 males and 75 females,with an average age of 85.2 years (from 80 to 97 years).Their fractures were Garden type Ⅲ (72 cases) and Garden type Ⅳ (48 cases).The time from injury to operation averaged 5.1 days.The patients were divided into a fracture group and a non-fracture group according to the presence or absence of the periprosthetic fracture.The general data of the 2 groups were compared;multivariate logistic regression analyses were done to indentify the influencing factors associated with periprosthetic femoral fracture.Results The 120 patients obtained a mean follow-up of 26.1 months (from 13 to 48 months).Periprosthetic femoral fracture occurred in 11 cases,giving an overall incidence of 9.2％ (11/120).Compared with the non-fracture group,the average age was significantly older,the incidence of past fractures was significantly higher,significantly more types of uncemented stem were used,and American Society of Anesthesiologists (ASA) grading was significantly more severe for the fracture group (P ＜ 0.05).There were no significant differences between the 2 groups concerning the general data (P ＞ 0.05).Multivariate Logistic regression analyses revealed that age [OR =1.268,95％ CI (1.059,1.517),P =0.010] and type ofuncemented stem [OR =0.072,95％ CI (0.008,0.625),P =0.017] were independent risk factors for periprosthetic fracture.Conclusions The incidence of periprosthetic femoral fracture in the elderly patients may be high following HA for femoral neck fractures.Since age and uncemented stem may be independent risk factors for periprosthetic femoral fracture,surgeons should pay enough attention to them in clinic.