Objective: Study the relationship between the activity and structural parameters of aminoquinolines. Methods: A new type of topological indices, called the hydrogen - association classified molecular electrongativity - distance vector ( H - MEDV) developed in our laboratory, has been used to describe the chemical structure of 40 aminoquinolines. Reasonable molecular modeling results were achieved by a multiple linear regression ( MLR). Results; For two sets of aminoquinolines, the correlation coefficient ( R) between the estimated and the observed activities were 0.920 and 0.910 respectively. Conclusion; Hie results suggest the estimation stability and predictive ability of the model based on the H - MEDV descriptors.

This article investigates the awareness and various concerns of the policy which released by the government of rural cooperative medical care and health insurance policies for farmers and migrant workers.Moreover,a number of recommendations are put forward to eliminate these concerns,and guarantee the proper implementation of government's care policy.

Objective To evaluate the value of MRI used in the shoulder pain. Methods 34 cases with complaint of shoulder pain underwent MR scanning, the findings of both MRI and surgery were analyzed comparatively.Results MRI of shoulder in 34 cases showed full-thickness and partial-thickness tears of the rotator cuff, tendinitis, glenoid labrum tear, synovitis, biceps tendinitis, biceps dislocation and tumors. In 13 patients received arthroscopy or operation. MR findings of 11 cases were correlated to surgical result, one case diagnosed as tendonitis by MRI was showed partial-thickness tears of the rotator cuff during operating, one case in which the MRI showed normal was found biceps tendonitis by arthroscopy. Conclusion MRI can show complex anatomy of shoulder and has high accuracy in diagnosis of shoulder pain, it is a value method for shoulder pain.

collagen type Ⅰ.The epithelial surfaceof HABM was significantly better in promoting forebrain cells growth than thestromal surface(P

Objective To evaluate the relationships between serotypes of U.urealyticum intrauterine infection with outcome pregnancy and placental villus ultrastructural transform.Methods The placental tissues from 45 cases with spontaneous abortion,34 cases of high-risk pregnancy,including 6 premature delivery and 28 PROM patients(study group)and 51 normal pregnant patients(control group)were isolated,cnltured and performed.Serotypes of U.urealyticum were identified by a metabolic inhibition test.The positive cases placental tissue of U.urealyticum were preformed ultrastructural observation by electron microscope.Results Total positive rate 63.3%(50/79)in the study group,showing significant difference from that of control group(47.1%,24/51,P

Objective To observe the effect of ammonia silver nitrate on early caries.Methods 100 cases of children with early caries treated in our hospital from April 2015 to December 2016 were admitted to conduct research and analysis.The children were divided into the control group and the observation group by double blind,and random method 50 cases in each group.The control group was treated with caries removal,the observation group was treated with carious worn out joint ammonia silver nitrate.After treatment, the two groups of children suffering from dental pulp tissue TNF-a, IL-8 concentration, the recurrence rate, filling rate falling, the rate of suffering from pulpitis, pain conditions were analyzed.Results After treatment, the observation group with TNF-α, IL-8 concentrations were lower than the control group(t=21.961,t=23.836), the difference was statistically significant (P<0.05);The recurrence rate, suffering from pulpitis rate was significantly lower than the control group, and the difference was statistically significant (P<0.05);the observation group after treatment in children with severe pain and painless was significantly lower than the control group, and the difference was statistically significant (P<0.05).Conclusion Ammonia silver nitrate is an effective and safe treatment for early caries, which can relieve the pain and discomfort in children, it is a safe and effective method of treatment and should be promoted and used in the clinic practice.

Objective: According to the relevant principles of blood volume monitoring and monitoring methods, to apply online monitoring data information management in the blood volume monitoring for hemodialysis patients. Methods:through the relevant technical form and its function of monitoring data to study and analyze of treatment parameters of hemodialysis patients described in online blood volume monitor, to achieve data analyzed by using information management system. Results: the blood volume can be visually monitored during the hemodialysis to understand changes in the blood volume of hemodialysis patients, it helps to avoid the occurrence of hypotension and evaluation of patients with dry weights during the hemodialysis. The data can be downloaded, exported, and edited at the same the while fully access to treatment data by effective using of blood volume monitoring data management system (BVMS), in order to achieve data analyzed by using information management system. Conclusion:by understanding the correlation betweenΔBV and blood pressure screen composition can help to speculate the reasons for drop in blood pressure;by combining the examination data can help to control dry weight management, by fully accessing to data can help to manage hemodialysis patient data information management system, and according to patients expression and display of blood volume monitoring to determine the appropriate amount of ultrafiltration, adjust dry weight and develop an appropriate treatment plan.

BACKGROUND: Bone marrow mesenchymal stem cells (BMSCs) as a genetic carrier are beneficial to keep a genetic stability of exogenous gene. BMSCs can transfer human vascular endothelial growth factor (hVEGF) gene into the myocardial infarcted area. It is possible to obtain a cooperation of gene and cell therapy. OBJECTIVE: To investigate the angiogenesis at myocardial infarcted area of rats undergoing the transplantation of BMSCs transfected with hVEGF gene and in vivo expression of VEGF gene. DESIGN, TIME AND SETTING: A randomized control experiment was performed at the Institute of Paediatrics of Hunan Children's Hospital and Central Laboratory of Xiangya Second Hospital of Central South University from 2004 to 2007. MATERIALS: Male clean inbred strain Wistar rats were selected in the study. Rat models of myocardial infarction were established by ligation of coronary artery. METHODS: BMSCs were harvested from rats by density gradient centrifugation and adherent culture. Plasmid PeDNA3. 1-hVEGF 165 were transfected at 80%-90% cell confluence. Rat models of myocardial infarction, constructed by ligation of the left anterior descending coronary artery, were randomly divided into 4 groups. 2 weeks after the ligation,BMSCs transfected with hVEGF gene were injected into rat models in the combination group at the myocardial infarction zone. BMSCs were injected into cell group. Liposome-pcDNA3.1-VEGF165 DNA compound was injected into gene group. Culture medium was injected into control group. MAIN OUTCOME MEASURES: Capillary density and VEGF gene expression in the myocardial infarcted area of rats in each group four weeks after injection. RESULTS: Capillary density was the highest at rat myocardial infarcted area in the combination and gene groups, followed by the cell group (P=0.001, 0.029) and the control group (P=0.028).Reverse transcriptase-polymerase chain reaction (RT-PCR) showed that the hVEGF165 gene was expressed in the combination, gene, cell and control groups from higher level to lower level in order. CONCLUSION: BMSCs as the vector of VEGF gene are beneficial to its stable expression.Transplantation of BMSCs transfected with hVEGF gene is helpful for angiogenesis at myocardial infarcted area of rats. Its outcome is better than gene or cell therapy alone.

Objective To compare the content variation of catalpol between single and mixed decocting Rehmanniae Radix and Paeoniae Radix alba; To preliminarily explore the variation rules of compatibility of Rehmanniae Radix and Paeoniae Radix alba. Methods HPLC method was used with Waters Symmetry C18 column (4.6 mm×150 mm, 5 μm), flow rate of 1.0 mL/min, detection wavelength of 210 nm, column temperature at 25 ℃. The mobile phase was acetonitrile-0.01% phosphoric acid in water isocratic elution system. Results Catalpol regression equation was Y=270 054X-57 724, r=0.999 6, showing good linear range among 0.225–7.2 μg. The average recovery rate was 101.15%, RSD=0.39%. The average content of catalpol in the mixed decoction of Rehmanniae Radix and Paeoniae Radix alba was 18.66%more than the single decoction of Rehmanniae Radix. Conclusion The content of catalpol in mixed decoction is higher than the single decoction of Rehmanniae Radix and Paeoniae Radix alba. The mixed decoction is favorable for the dissolving of catalpol from the medicine. The compatibility of Rehmanniae Radix and Paeoniae Radix alba is rational.

Objective To clone and construct eukaryotic expressing vector of bcr-abl fusion gene and to express the gene in the mammal COS-7 cell lines. Methods bcr-abl fusion gene was amplified from human chronic myeloid leukemia (CML) K562 cell lines by RT-PCR and the fragment of cDNA was retrieved,purified and cloned into the pEGFP-N3 eukaryotic expressing vector. After the selection of the positive clone and by restriction enzyme analysis and DNA sequencing, the correct plasmid was transfected into COS-7 cell lines and observed the transient expression. Results A 874 bp DNA fragment was amplified by RT-PCR. The sequence analysis showed it was consistent with bcr-abl gene of GeneBank. RT-PCR, Western blotting analysis provided strong evidences that bcr-abl gene was expressed successfully in transfected COS-7 cells.Conclusion The eukaryotic expressing vector of bcr-abl fusion gene was constructed, it will lay the foundation for further study of bcr-abl gene in the diagnosis and treatment of CML.