Objective: To investigate the postoperative change of concentration of CD_(41) in peripheral platelet following intra-operative polygeline administration. Method: Sixty-eight patients were randomly divided into studied and control groups. The patients in studied group were infused with 1 000ml polygeline whereas 1 000ml ringer's solution in control group. The concentration of CD_(41) in peripheral platelet was measured with immunocytometry before surgery, one hour, one day, two and five days after surgery. Result: There was no statistical difference in CD_(41) level between the two groups before surgery, CD_(41)values one hour and one day after surgery were obviously decreased in studied group compared with these in control group (P

Objective To evaluate the diagnostic value, patients' tolerance and complications of double-balloon enteroscopy (DBE) in the diagnosis of small intestinal diseases. Methods During May 2003 to July 2005, a total of 68 patients (36 men, 32 women; mean age of 52. 6 years, range 15-78 years) with suspected small intestinal diseases were performed double-balloon enteroscopy (36 via mouth, 25 via anus and 7 via both mouth and anus according to suspected lesion location). Among them, obscure recurrent gastrointestinal bleeding was found in 39 cases, incomplete small intestinal obstruction in 7 cases, chronic abdominal pain in 14 cases, and chronic diarrhea in 8 cases. Results Approximately one half to three-fourth of the entire small intestine was observed by each approach in all cases except for 3 cases of severe intestinal stricture. The observation of the whole small intestine was finished by the combination of both oral and anal approaches in 7 cases. The appropriate use of X-ray made the enteroscopy easier and more helpful to determine the extent and location of the lesions. The lesions were found in 41 of the 68 patients, with a total positive rate of 60. 3%. The diagnostics yields was 26/39(62. 6%) in obscure recurrent gastrointestinal bleeding, 5 in incomplete intestinal obstruction, 6/14(43%) in chronic abdominal pain and 4 in chronic diarrhea, respectively. Fifty-seven cases (83. 8%) tolerated well without anesthesia while 11 cases received propofol anesthesia. No procedure-related severe adverse events or severe complications such as hemorrhage or perforation occurred in all cases. Conclusion Double-balloon enteroscopy is a well-tolerated and safe diagnostic approach with a high diagnostic yield in small intestinal diseases.

Objective To investigate the oxidative stress,microinflammation and their relationship in patients with MHD(maintenance hemodialysis)and to evaluate the effect and safety of vitamin E supplementation in patients with MHD.Methods Fifty-eight patients with MHD and 20 healthy volunteers were involved in this study.The patients were administered 400 mg/d of vitamin E for 6 weeks.The levels of oxidative vitamin E(Vit E),malondialdehyde(MDA),advanced oxidation protein product(AOPP),superoxide dismutase(SOD),activity of glutathione peroxidase(GSH-PX),microinflammation cytokines C-reactive protein(CRP),interleukin-6(IL-6),interleukin-10(IL-10) and tumor necrosis factor(TNF-?)in plasma were measured at the beginning and the end of administering Vit E.Results 400 mg/d of Vit E supplementation decreased the levels of plasma AOPP,MDA,CRP,TNF-?(P

Objective To explore the common factors' influence on serum gastrin level,and reveal the value of serum gastrin in the diagnosis of gastric precancerous lesions and gastric cancers.Methods Serum gastrin level of 3 906 serum samples was detected by ELISA.Results Serum gastrin level of patients over 60 years old was distinctively higher than that of other age groups.Serum gastrin level in antral diseases was higher than that in diseases of the body.HP positive group was higher than the negative one.The level progressingly increased from normal group to superficial gastritis and to gastric erosion or ulcer;and it decreased from gastric erosion or ulcer to atrophic gastritis,while it increased from atrophic gastritis to dysplasia,and it decreased from dysplasia to gastric cancer.Serum gastrin level was distinctively lower in gastric cancer than in other groups.Conclusion For people over 60 years old,their serum gastrin level has the tendence to increase.The location of gastric disease is an important factor affecting serum gastrin level.HP can increase the serum gastrin level.During gastric precancerous stage,the serum gastrin level may gradually increase with the diseases' progression,and decrease when gastric cancer is formed.Serum gastrin is a good marker to differentiate benign and malignant gastric diseases.

Objective To observe the clinical effect and adverse reaction of oxycodone sustained-release tablet and morphine tablet in dose titration therapy on moderate and severe chronic cancer pain. Methods Sixty patients suffering from moderate and severe cancer pain, without using opioid drugs, were divided into oxycodone sustained-release tablet group and morphine tablet group by random digits table method with 30 cases each. The patients in oxycodone sustained-release tablet group were administered 10 mg oxycodone sustained-release tablet every 12 h, and the patients in morphine tablet group were administered 5 or 10 mg morphine tablet whenever needed. The total dose of opioid drugs was acquired after 24 h, and was converted into equal dose of oxycodone sustained-release tablet. The condition of pain control and adverse reaction were observed and recorded in a week. Results During the titration, the number of daily outbreak pain and daily medication in oxycodone sustained-release tablet were significantly lower than those in morphine tablet:(1.27 ± 1.53) times vs. (4.87 ± 1.98) times and (3.37 ± 1.78) times vs. (5.10 ± 2.20) times, and there were statistical differences (P<0.05). At the first day after titration, the pain relief rate of oxycodone sustained-release tablet was significantly higher than that of morphine tablet:83.33%(25/30) vs. 60.00%(18/30), and there was statistical difference (P<0.05). While at the third day after titration, there was no statistical difference in the pain relief rate between 2 groups (P>0.05). At the first day after titration, the incidence of daily outbreak pain in oxycodone sustained-release tablet was significantly lower than that in morphine tablet:23.33%(7/30) vs. 53.33% (16/30), the rate of reaching steady pain control state was significantly higher than that in morphine tablet: 86.67% (26/30) vs. 63.33% (19/30), and there were statistical differences (P<0.05). There were no statistical difference in the incidence of daily outbreak pain, rate of reaching steady pain control state at the third day after titration, time of reaching steady pain control state, and incidence of adverse reaction (P>0.05). Conclusions The pain relief rate and side effect of oxycodone sustained-release tablet is similar to that of morphine tablet in dose titration therapy on moderate and severe chronic cancer pain, but analgesic effect is faster than morphine tablet. Oxycodone sustained-release tablet decreases the number of outbreak pain and relieves patients′ pain in the titration process. Oxycodone sustained-release tablet may have advantage of time and effect, which is worth to be widely used in clinical therapy.

OBJECTIVETo assess the DNA damage of copper 8-quinolinolate (CuQ) and to elucidate the plausible mechanisms.METHODSHepG2 cells were treated with CuQ0-4 μmol·L-1 for different time,DNA damage was measured by Comet assay.Catalase (CAT) activity,glutathione(GSH) level and thiobarbituric acid reactive substances (TBARS) were measured.NF-κB was examined using Western blotting.8-Hydroxydeoxyguanosine (8-OHdG) was measured by immunoperoxidase staining analysis.RESULTSCuQ 0.5 -4 μmol·L-1 caused significant increase of DNA migration in HepG2 cells.CuQ significantly decreased levels of GSH and activity of CAT in HepG2 cells (P ＜0.05).Moreover,CuQ significantly increased accumulation of the p65 subunit of NF-κB into nucleus,levels of lipid peroxidation product TBARS and the formation of 8-OHdG (P ＜0.05).The intracellular GSH level was modulated by pre-treatment with buthionine-(S,R)-sulfoximine (BSO),depletion of GSH in HepG2 cells pre-treated with BSO dramatically increased susceptibility of HepG2 cells to CuQ-induced DNA damage.CONCLUSIONCuQ exerts DNA damage by oxidative stress and increases accumulation of p65 subunit of NF-κB into nucleus in HepG2 cells.

Objective To explore the ability of ABCD2 score + Low density lipoprotein (LDL)(ABCD2L) in predicting early stroke risk after transient ischemic attack (TIA) . Method A total of 165TIA patients were evaluated and classified according to ABCD2 score and ABCD2L score. The occurrences of cerebral infarction were observed at 2th day or 7th day. ROC curve was used to compare the predictive vaule of two scores. Furthermore, according to these two scores, these patients were classified into three risk groups (low, moderate and high ), observed their stroke rate , and compared the differences of three groups with x2 test. Results The two-day risk of stroke was 13.33% and the seven-day risk of stroke was 20. 0% in 165 patients. When the occurrences of cerebral infarction were observed in two days, the area under the curves (AUC) of ABCD2 and ABCD2L was 0. 76 and 0. 80; observed in seven days, the AUC of two scores was 0. 73 and 0. 79. According to the risk stratification of ABCD2 score, in three risk groups,the two-day risk of stroke was 1.9%, 14. 9% and 30. 8%; the seven-day risk of stroke was 3. 8%, 21.8%and 46. 2% ( P ＜ 0. 05 ) . According to the risk stratification of ABCD2 L score, the two-day risk of stroke was 0%, 7. 8% and 31.1% ; the seven-day risk of stroke was 0%, 12. 6% and 44. 4% ( P ＜ 0. 05 ).Conclusions The predictive accuracy of the ABCD2L score is significantly higher than that of ABCD2 score. Furthermore, individuals at high early risk of stroke after TIA can be identified according to the risk stratification of ABCD2L score.

Objective To explore the characteristic and pattern of children injury of Zhejiang Province,and provides scientific evidence for the prevention and control strategy of children injury. MethodChildren diagnosed as injury at emergency department in sentry hospitals (Tnere are 5 monitoring sites for injury surveillance in Zhejiang in 2005: Jinhua, Shengsi, Tongxiang, Yinzhou dislrict of Ningbo, Hailing; each monitoring site has 3 sentry hospitals: one county level hospital, one town center hospital and one town level hospital) from January to December,2005 were investigated by trained doctors or nurses in emerncy department. The investigation included basic information, when injury happened, where injury happened and type of injury. Children were investigated when they were conscious, and their parents or accompanies could answer if they were not able to. The contents of investigation were kept private. The distribution of variables which included sex, age, when injury happened, where injury happened, why injury happened and severity level were described mainly with proportion and means, and chisquare test was used to analyze the proportion difference. RemitsA total of 1 794 injury cases were recorded in 2005 among which 67.56% were male and 32.44% were female. The majority were native people and the average age was 7.33±3.84 years old. The injury occurred mostly at home and was of high frequent occurrence from April to November. The most common causes of injury were tumble (47.94%).traffic accident (17.17%) and blunt injury(10.26%). The injury happened mostly during spare time(67.66%), and then school aetivities(11.25%)ant]sports activities(9.96%). Totally 90.33% of the injured children went home after been treated, 1.40% stayed for obsrvafion, 7.04% were in hospital, 0.56% were transferred to other hospitals, 0.22% died and 0.45% unknown. The slight superficial injury and moderate injury were the most common injuries. The most common three types of injury were haemaloma and stasis injury(31.29%), superficial abrased wound(23.69%) and bone fracture(8.67%). Among the injury, 39.47% were head injury, 25.80% were upper limb, 28.55% were lower limb, 4.81% were trunkand 1.38% wereothers. Conclusions The most common three types of injury in Zhejiang province were haemawma and stasis injury, superficial abrased wound and bone fracture. The main reasons of injury were falling injury,traffic accident injury and blunt injury. The injury happened mostly during spare time, and scbool activities and sports activities. Thus, safety education and safety protection should be strengthened to reduce children injuries.

Objective To know the effects of training for community residents about cardiopulmonary resuscitation.Methods 60 community residents were trained by CPR with varied training methods,evaluated the effects by the ABC for life questionnaire.Results The knowledge of cardiopulmonary resuscitation among the trained community residents were better than before expect 4 aspects.Conclusions The knowledge of cardiopulmonary resuscitation in community residents was not enough,systemic training can effective promote their skills of CPR.

Objective To explore the psychological status of patients after hand tenolysis and the pertinent nursing countermeasures. Methods 47 patients after hand tenolyais were selected and investigated their psychological status and relevant influencing factors with serf-designed questionnaire and symptom checklist-90(SCL-90). Besides, corresponding psychological nursing were applied. The SCL-90 results of Chinese Norm were adopted to undergo comparison with them and t test was chosen. Results On the first day after operation, the score of the investigation objectives was significantly different compared with that of the Chinese norm. After pertinent mental nursing, their symptoms alleviated, and no significant difference was seen between them. Conclusions There are obvious psychological obstacles in patients after hand tenolysis. The pertinent mental nursing has important effect on rehabilitation training and functional rehabilitation after hand tenolysis.