Objective To evaluate influence of benzo(a)pyrene on cell cycle of the human embryonic lung fibroblast at different synchronous stage. Methods Cell cycle distribution were detected by flow cytometry(FCM). Cells were synchronized at cycle G0 stage through 48 hours serum starvation and reenter cell cycle together at a synchronous state after resupplied with whole growth medium contain good serum. Benzo(a)pyrene with a series concentrations were directly used or metabolized active by traditional and modified metabolize active methods through preincubation of S9 and were treated as regent to HELF cells mostly synchronized at G1, S or G2-M stage respectively. Then cell cycle distribution changes were detected 12 h after treatment. Results Serum starvation(48 hours) could synchronize HELF at G0-G1 stage effectively and 10-12 h, 16-18 h, 22-24 h were cycle phase change distinctly time after serum restimulated. Cells were synchronized at G1, S and G2-M stage mostly. Benzo(a)pyrene influence cycle distribution little without metabolize active, while modified methods could metabolize active benzo(a)pyrene well and avoided disturbing effect of S9 on cell cycle in traditional method. Except 2 ?mol/L benzo(a)pyrene treated at 22 h after restimulated caused the percentage of cells at S stage increase, most treatment induced the percentage of cells at S stage decrease which was associated obviously with the increasing dosages. The percentage of cell at S stage decrease at 16 h were more remarkable than other times and the percentage of cell at G2-M increase correspondingly. While the percentage of cell at G1 increase and the percentage of cell at G2-M decrease were obsered when benzo(a)pyrene treated at 10 h and 22 h after restimulated. Conclusion Serum starvation 48 hours and restimulate can synchronize HELF at different stage effectively. Modified metabolize active method is suitable for cell cycle research. Primary influence of benzo(a)pyrene on cell cycle is the decreased percentage of cells at S stage, G1 arrest, G2-M arrest and G1 arrest were occurred when benzo(a)pyrene were treated at G1, S and G2 stage respectively.

Objective To discuss the application of breast surgery with radical surgery in the therapeutic effect and complications in patients with early breast cancer.Methods 110 early breast cancer patients with surgical treatment were randomly divided into two groups,each group in 55 cases,the control group was given radical surgical treatment,the observation group was given breast conserving operation,surgery were recorded in patients of the two groups.Results The results of the observation group showed operative incision length (4.02 ±1.15)cm,operation time (58.93 ±13.25)min,intraoperative blood loss (10.11 ±2.87)mLand length of hospital stay (10.74 ±2.17)d. The datas of control group showed surgical incision length (11.87 ±2.44)cm,operation time (95.72 ±26.78)min, intraoperative blood loss (85.27 ±14.28)mL and length of hospital stay (15.86 ±4.43)d,the differences were statistically significant (t =21.582,9.131,5.714,7.697,all P <0.05).Observation group occurred postoperative hand infection in 1 case,upper extremity edema in 2 cases,and no subcutaneous hemorrhage occurs.Control group had 6 cases of postoperative hand infection,10 cases of upper limb edema,and subcutaneous hemorrhage in 5 cases,the differences were statistically significant (χ2 =4.852,5.986,5.238,all P <0.05).3 months after operation,datas of observation group showed physiological state (54.18 ±6.28)points,psychological state (59.27 ±5.82)points,environmental conditions (68.45 ±7.68)points and social function (57.46 ±5.97)points.Physiological state of the control group after 3 month was (65.69 ±9.11)points,while psychological state was (68.31 ±7.33)points,environmental conditions was (78.17 ±10.54)points,social function was (68.77 ±7.15)points,the differences were statistically significant (t =7.714,7.163,5.527,9.004,all P <0.05).Conclusion Breast surgery application in patients with early breast cancer can shorten the operation time,reduce intraoperative bleeding and flow rate,shorten the length of hospital stay, reduce complications and improve postoperative quality of life,it is worth popularizing in clinical application.

Objective To optimize the extraction technology of Morchella polysaccharide.Methods With GIM5.69(Morchella esculenta(L.) Pers.) and GIM5.29(Morchella elata) as the research object,the difference of polysaccharide yield with lixiviation in polysaccharide extraction between ultrasound and enzyme was observed.At the same time,the difference of polysaccharides yield of these two Morchella was compared.Result Ultrasound and enzyme both can improve the polysaccharides yield,and the effect of 10 min of ultrasound was the best.The polysaccharides yield of GIM5.69 was higher than that of GIM5.29,which means different strain has different polysaccharides yield.Conclusion The best extraction condition is 10 min of ultrasound at the frequency of 40 kHz,heating 150 min at 90 ℃ and lixiviation twice.

HDL or apolipoprotein A-Ⅰ,the major protein of HDL particles,protect host against vascular endothelial dysfunction.HDL binding to scavenger receptor-BI activates eNOS for increasing NO.SR-BI and Endothelial lipase(EL) play a critical role in the regulation of plasma HDL.Incubation of HDL with vascular endothelial cells promotes prostacyclin synthesis.Some proteins such as paraoxonase that cotransport with HDL in plasma,have antioxidant properties.HDL inhibits TNF-? dependent expression of adhesion molecules in vascular endothelial cells and interferes with apoptosis,proliferation and migration of vascular endothelial cells.

Objective To investigate the pathogenesis and clinical features of ankylosing spondylitis (AS) concurrent with multiple myeloma (MM).Methods The clinical characteristics,pathogenesis,diagnosis and treatment of 3 cases who were diagnosed as AS concurrent with MM were analyzed and relevant literatures were reviewed.Results MM was confirmed in patients who had long-term stable AS.A wide variety of manifestations,including persistent pain in spine and thorax,pathologic fracture,anemia,kidney damage and nervous system abnormalities were frequently presented.These patients were insensitive to nonsteroidal anti-inflammatory drug treatment,and they had a unfavorable prognosis.Conclusion Patients who are diagnosed as AS concurrent with MM can present with a variety of clinical features.Manifestations which are difficult to explain clinically by AS and poor response to treatment especially when the patients have longterm stable AS are potential clues for MM.

Hedysari polysaccharide (HPS) is the main component of hedysarum polybotrys. HPS can inhibit tumor cell proliferation, block the cell cycle in the G2-M period, modulate apoptosis via the mitochon drial pathway, and improve the red blood cell innate immunity and T lymphocyte immune function. HPS can relieve low immunity caused by chemotherapeutics.HPS plays anti-tumor effects by these ways mentioned above.

Objective To summarize the clinical,radiographic and genetic features of a family with metatropic dysplasia,in order to improve the level of understanding and diagnosis of this disease.Methods The proband,a one-year old boy,was diagnosed as metatropic dysplasia.His mother was 26 years old with mildly phenotype.Their clinical features and bone X-ray findings were analyzed.The DNA samples of the proband and his parents were collected.The coding exons and flanking introns regions of transient receptor potential vanilloid 4 (TRPV4) gene were amplified by polymerase chain reaction (PCR) and analyzed by DNA automatic detector.The pathology,diagnosis,treatment and prognosis were expounded.Results The symptoms of the boy were characterized by short extremities,a short trunk with progressive kyphoscoliosis,and craniofacial abnormalities that include a prominent forehead,midface hypoplasia,and a squared-off jaw.His motor development was slightly delayed.Mental development was normal.Bone X ray of the boy showed platyspondyly and severe metaphyseal enlargement with shortening of long bones and irregularities and delayed ossification of epiphysis.The patient and his mother were heterozygous for the nucleotide substitutions c.2396 ＞ T (p.P799L) in TRPV4 gene.Conclusions The patient and his mother with metatropic dysplasia were diagnosed with TRPV4 gene analysis.The patient showed typical clinical features.His mother was mild.Metatropic dysplasia had significantly clinical heterogeneity.Gene analysis is helpful for the diagnosis.

Objective This paper will study the effect of the replantation and full crown prosthesis in the treatment of refractory molar periapical disease as well as their related factors .Methods The retrospective analysis will be used in this paper.There were 98 patients who were diagnosed as refractory molar periapical disease in our dental clinic from 2012 to 2015.These 98 cases are divided into two groups:the study group and the control group .This paper will make a comparison of the study group which represents the replantation and full crown prosthesis in the treatment and the control group which re -presents endodontic therapy .Results Through the comparison , the average effective rate of the study group is 93.9%while the control group 65.3%.There is a significant difference between two groups (P<0.01) and the rate of the study group is higher than that of the control group .Conclusion Through the analysis , this paper finds that the joint optimization of replan-tation and full crown prosthesis can provide an unconventional approach to treat refractory periapical of teeth and effectively preserve the tooth .

Objective To observe the growth inhibition effect of sustained-release fluorouracil implantation in nude mice with human colorectal carcinoma and to discuss its influence on caspase-3 expression .Methods The colorectal carcinoma subcutaneous transplanted model with LoVo cell in BALB/c nude mice was established and divided into 4 groups randomly :the tail intravenous injection of PBS (A group) ,the tail intravenous injection of 5-FU group (B group) ,the intratumor injection of 5-FU group (C group);and the implant of sustained-release fluorouracil group(D group) .After 15 d of medication ,the tumor weight and tumor volume were recorded ,the tumor inhibiting rates were calculated .The tumor tissues were observed by HE staining and detected the expression of caspase-3 by immunohistochemistry (IHC) .Results Sustained-release fluorouracil could suppress the transplantation tumor growth significantly ,the tumor weight and tumor volume in the D group had statistical differences compared with the A ,B and C groups(P<0 .05) ,which could up-regulated the expression of caspase-3 ,the differences between the D group with the A ,B and C groups (P<0 .05) .Conclusion Sustained-release fluorouracil can inhibit the grow th of colorectal carcinoma transplantation tumor more effectively than conventional fluorouracil and up-regulate the expression of caspase-3 ,which prompts that sustained-re-lease fluorouracil may play the anti-tumor role by inducing the tumor cell apoptosis via apoptosis pathway .Its definite tumor-inhibi-ting effect deserves to be promoted in clinical chemotherapy .

Objective Pathologically, sometimes it is rather difficult to make differential diagnosis among hepatocellular, cholangiocellular and mixed type of hepatic tumors. The aim of this study was to analyze the immunohistochemical features of mixed type of hepatocarcinoma and to propose some useful diagnostic criteria. Methods Fifteen cases of the hepatic tumor were examined by routine histological and immunohistochemical methods with S P technique. Results The mixed type of hepatic tumor accounted for 3.39% of all cases of primary hepatocarcinoma in the same period. Histologically, all the mixed type of hepatic tumors revealed some characteristics of hepatocellular and cholangiocellular carcinomas, one of them was collision tumor. Immunohistochemically, 10 cases of the tumor were positive for ? fetal protein(AFP) in the hepatocellular carcinoma area, while all 15 tumors were negative for AFP in all cholangiocellular carcinoma areas. Cytokeratin(CK8) was positive in the hepatocellular carcinoma areas of all mixed tumor, whereas positive in cholangiocellular carcinoma areas of 13 cases; CK19 was positive in cholangiocellular carcinoma areas of all the tumors, while positive in hepatocellular carcinoma areas of 13 cases. Bcl 2 gene product was positive in 66.7% of this tumor, in which it was mainly expressed in cholangiocellular tumor area and in hepatocellular areas of five cases. AB/PAS stain for mucin was positive in cholangiocellular tumor regions of all the tumors. Conclusions Diagnosis of mixed hepatocarcinoma should be made mainly based on combined analysis of the data from pathological features, immunohistochemical phenotypes and histochemical stain for mucinous material.