Three-dimensional (3D) fluorescence spectra and thin layer fluorescence chromatogram of common Camptotheca fruit (CCF) crude drug, camptothecin (CPT) and 10-hydroxycamptothecin (HCPT) have been studied, and a novel fluorimetric method for determination of CPT in CCF crude drug has been established. In 3D fluorescence spectra, CPT presented 3 fluorescence peaks with excitation wavelengths lambdaex of 215, 255 and 365 nm, separately, and all peaks with emission wavelength lambdaem of 430 nm. HCPT presented 4 fluorescence peaks with lambdaex of 220, 265, 325 and 375 nm, separately, and all peaks with lambdaem of 555 nm. The fluorescence of CPT is much stronger than that of HCPT. Comparison of 3D fluorescence spectra and analysis of thin layer fluorescence chromatogram revealed that the main fluorescent component of CCF is CPT. HCPT and other components basically do not interfere with the fluorescence of CPT. Under the condition of pH 3.0-6.7, CCF aqueous solution can produce strong and steady fluorescence. Using methanol as solvent, the extracting solution of CCF was prepared, and diluted properly with water, then measured fluorescence intensity at lambdaex/lambdaem = 365/430 nm to determine the content of CPT. A linear calibration curve covered the concentration range 0.002 35-0.235 microg x mL(-1). The regression equation was IF = 9 + 30,844 c, with correlation coefficient r = 0.999 (n=9). The method has been applied to the analysis of CPT in a CCF sample, with a result of 0.127% and a spiked recovery rate of 102%. The reliability of the method has been verified by a thin layer chromatography-fluorescence scanning method. Experimental results demonstrated that this method can be used for quality evaluation of CCF crude drug.

Objective To study the angiographie characteristics of coronary heart disease (CHD) patients with type 2 diabetes mellitus.Methods 292 patients with CHD underwent a selective angiography were selected,the characteristics of coronary arteriongraphy were compared in CHD patients with type 2 diabetes mellitus and nondiabetic group.Results In type 2 diabetes mellitus group,the degree of stenosis was higher than that in nondiabetic group (78.01% vs 52.32% )(P < 0.05 ), and the rate of three-vessel disease was higher than that in nondiabetic group (59.57% vs 36.42% )(P <0.05).Conclusion The angiographic characteristics were different in CHD patients with or without type 2 diabetes mellitus.Patients with type 2 diabetes mellitus was associated with more severity of lesion and more lesion vessel.

Objective:To observe the effects of osteoprotegerin on osteoclasts in the occlusal tissue during tooth eruption in dog and analyze the mechanism. Methods: Six 7-day-old brood dogs were divided randomly to control and osteoprotegerin groups. The dogs in osteoprotegerin group were injected with osteoprotegerin for 3 days 1.5 mg/(kg?d). 5 days after injection the dogs were sacrificed and mandible were taken out for paraffin slice. The number of the osteoclasts in the occlusal tissue up the third mandibular premolar was detected by tartrate-resistance acid phosphatase(TRAP) staining. Immunohistochemistry was used to examine the expressions of RANKL. Results: The number of osteoclasts in osteoprotegerin group was less than that in the control group (P

Objective To investigate the effect and mechanism of the icaritin on the human cronic myeloid leukemia K562 cells. Methods The K562 cells at logarithmic growth phase were divided into the control group and the icaritin group. The cells in the control group were normally treated and the cells in the icaritin group were incubated with 8 μmol/L icaritin. Methyl thiazolyl tetrazolium (MTT) method and flow cytometry were used to examine the proliferation and apoptotic changes in the two groups after incubation for 72 h, respectively. Gene expression of p85 and Akt were detected by RT-PCR. The protein changes of p85, Akt, p-p85, p-Akt cleavage-caspase-3 and caspase-3 were detected by Western blot. Results Compared with the control group, the proliferation rate of K562 cells in the icaritin group was significantly decreased (P<0.05), however the apoptotic rate of K562 cells and the expressions of p-p85, p-Akt, cleavage-caspase-3 in the icaritin group were significantly increased (all P< 0.05), but the expressions of p85 mRNA, Akt mRNA and caspase-3 protein had no difference (all P> 0.05). Conclusion Icaritin could induce the proliferation and promote the apoptosis of K562 cell, and its mechanism may be achieved through activating the PI3K-Akt signal transduction pathway.

Objective To investigate the levels of transformation growth factor-β1 (TGF-β1),vascular endothelial growth factor (VEGF) and insulin growth factor-Ⅰ (IGF-Ⅰ) role in the pathogenesis of serum of patients with combined pulmonary fibrosis and emphysema (CPFE).Methods Recruited 20 patients with CPFE,40 cases with idiopathic pulmonary interstitial fibrosis (IPF) and 40 cases with emphysema who were admitted to our hospital during July 2011 to February 2012.Serum levels of TGF-β1,VEGF and IGF-Ⅰ were detected by ABC-ELISA.Results Serum levels of TGF-β1 and IGF-Ⅰ were significantly higher in patients with CPFE and IPF than these in patients with emphysema (TGF-β1:(160.73 ± 40.62) ng/L vs.(167.35 ± 42.82) ng/L vs.(128.75 ±35.77) ng/L; IGF-Ⅰ:(179.65 ±60.73) ng/L vs.(192.32 ±58.75) ng/L vs.(148.73 ±49.67) ng/L,P ＜ 0.05 or P ＜ 0.01).The IPF group had significantly higher serum level of VEGF than the emphysema group ((506.12 ±82.37) ng/L vs.(437.31 ±62.58) ng/L,P ＜0.01).Serum levels of TGF-β1 and IGF-Ⅰ in CPFE and emphysema groups were positively correlated (r =0.885,0.918 respectively,P ＜ 0.01).Smokers in the CPFE group had significandy lower level of serum VEGF than those who did no smoke ((406.19 ± 66.94) ng/L vs.(482.88 ± 79.91) ng/L,t =-2.287,P =0.035).Conclusion Serum level of VEGF increased significantly in the IPF group,suggesting the participation of VEGF in pulmonary interstitial fibrosis.IGF-Ⅰ involved in the pathogenesis of pulmonary interstitial fibrosis.TGF-β1 and IGF-Ⅰ have a positive linear regressive relationship,which indicates that they may work synergistically in the process of the fibrosis.

Objective To explore the correlation between obstructive sleep apnea syndrome(OSAS)and developing hypertension.Methods Clinical data of 124 OSAS patieats with high blood pressure were retrospectirely,and according to the degree of hypoxemia they weredivided into three groups,monitoring of the night and 7h polysomnography figure,compare each group,woke up blood pressure changes before bedtime.Results 124 patients with hypertension OSAS clinical manifestation:snoring 124 cases(100.0％),daytime sleepiness 74 cases(59.7％),wake up in the night to suppress 49 cases(39.5％),night apnea 39 cases(31.5％),woke up oppressed 26 patients (21.0％),since morning headache 19 patients(15.3％).OSAS merger hypertension,light,medium,heavy hypoxemia between groups of blood pressure,blood pressure after come before a statistically significant difference(P ＞0.05).Conclusion Hypoxia was associated with high blood pressure.

AIM: To study the pharmacokinetics of ? Asarone in the volatile oil from Acorus. Tatarinowii Schott. in mice. METHODS: We developed a GC method for ? Asarone determination in blood. The GC system consisted of HP 35 capillary column with Helium as carrier gas, oven starting temperature set at 30℃ , then was raised to 220℃ at a rate of 6℃/min , stayed for 5 minutes, injector temperature set at 250℃ , detector temperature at 320℃ . Injection volume was 2.0 ?L with splitless inject mode. Methanol was used as solvent and ? naphthol as interior standard substance. CONCLUSION: The calibration curve in blood was linear in the range of 0.04?g/ml to 40?g/ml . The minimum detection limit was 0.04?g/ml . The recovery for ? Asarone in blood was 95.3% with RSD 3.92% . After oral administration of volatile oil from Acorus. Tatarinowii Schott., the intracorporal process of ? Asarone in mice accorded with 2 compartment model with 1st order absorption. 6.5min and 93.6min were obtained as t 1/2? and t 1/2? respectively.

Objective To evaluate the reliability of a series of non-invasive methods in the presurgical lateralization of mesial temporal lobe epilepsy(MTLE).Methods The results of the interictal scalp electroencephalogram(EEG)and ictal scalp EEG,clinical seizure symptom,MRI and interictal SPECT obtained from 40 patients with MTLE who had been followed up 1 to 4 years without seizure after anterior temporal lobectomy(ATL)retrospectively were analyzed in Xuanwu Hospital from May 2002 to May 2005.Results (1)Unilateral anterior temporal spikes were found on interictal EEG in 37(92.5%)patients,of whom 35(94.6%)were in accordance with the lateralization of epileptogenic focus.(2)Ictal scalp EEGs were recorded in 32 patients,from which epileptogenic foci were lateralized in 26 of the 32 patients(81.2%),the concordant rate being 96.2%.(3)Twenty-three patients(57.5%)had clinical seizure symptoms referring to lateralization,of whom 19(82.6%)had symptoms identical to the lateralization of epileptogenic focus.(4)Thirty-eight patients(95.O%)showed structural abnormalities of unilateral hippocampus or temporal lobes on MRI which were in accordance with the lateralization of epileptogenic focus in 37 patients.(5)Interictal SPECT was measured in 23 patients,which was identical with the lateralization in 18/22(81.8%).Conclusions Among a series of non-invasive methods in the presurgical lateralization of mesial temporal lobe epilepsy,SPECT is the most sensitive one,then sequenfly comes MRI,ictal scalp EEG,interictal scalp EEG and clinical seizure symptom.MRI is the most reliable one,then comes ictal scalp EEG,interictal scalp EEG,clinical seizure symptom and SPECT in a sequent order.

Objective To analyze the clinical data of Staphylococcus aureus sputum culture positive cases and the antimicrobial susceptibility of Staphylococcus aureus strains isolated from them and provide the evidence for clinical diagnosis and treatment. Methods Retrospectively analyze the clinical data of Staphylococcus aureus sputum culture positive cases of our hospital from January 2014 to December 2014. Results The Staphylococcus aureus sputum culture positive cases was 111 in all, while antimicrobial sensitive test was performed in 110 cases, including MSSA in 27 cases (24.55%), MSRA in 83 cases (74.45%), community infection MSRA in 13 cases (15.66%), hospital infection MRSA in 70 cases (84.34%). The cases were mainly from the department of respiratory diseases, department of neurosurgery, ICU, the department of neurology, accounting for 75.68%. The risk rate of community acquired MRSA in patients from nursing center were significantly higher than that of hospital infection MRSA and MSSA (P = 0.004). The antimicrobial susceptibility rate of community infection MRSA to clindamycin, gentamicin, erythromycin, azithromycin was higher than that of hospital infection MRSA. Conclusion The susceptibility rate in non-β-lactams of community infeciton MRSA was higher than that of hospital infection MRSA.