The rational prescription evaluation is important for improving the medical quality and ensuring prescription safety.For the last few years, the health authorities have issued a series of files in order to regulate clini-cal medical conducts, and reduce the irrational prescription.Hospitals are also paying more attention to the rational medicine evaluation.The evaluation is likely to be doubted and resisted for the difference of evaluation personnel, e-valuation personnel quality and evaluation criterion.Therefore, the smooth development of hospital rational prescrip-tion evaluation need to adopt effective management measures, which is connected with responsibilities, guaranteed by rewards and punishments and based on actualities, and establish various evaluation management mechanism to ensure continuous correction and updating at work.

Since the complain center of our hospital was founded in 2011, innovated the philosophy of “com-plain is gold”.It transformed mentality of the leaderships and employees to treat complaints fundamentally .Complain management was regarded as a strategic tool for the hospital to self -improve;Complain was regarded “the third eye”of recognizing the hospital management blind spots or service failure .At the same time, our hospital takes it as an im-portant means to construct the harmonious doctor -patient relationship sincerely .In the past three years , under the guidance of new ideas , our hospital has been innovating the complaint handling mechanism and improving work processes .And we have obtained better result in complaints management under the effort of all the staff .

Objective To evaluate the performance of the Sysmex XE-5000 analyzer for analyzing atypical lymphocytes ,baso-philic granulocytes and their abnormalities warnings .Methods A total of 197 specimens with both atypical lymphocytes and baso-philic granulocytes warnings and 914 specimens with single warning of atypical lymphocytes indicated by Sysmex-5000 blood cell analyzer were collected and inspected by microscope simultaneously .Results Using microscopic examination as a standard ,baso-philic granulocytes within the normal range ,the coincidence rate of samples with both atypical lymphocytes and basophilic granulo-cytes warnings was 64 .9% ,while the coincidence rate of samples with single warning of atypical lymphocytes was 72 .5% .The for-mer was significantly lower than the latter(P<0 .05) .Conclusion When Sysmex XE-5000 indicates atypical lymphocytes and baso-philic granulocytes simultaneously ,there is interference between each other .It should be combined with microscopic examination in order to reduce the probability of missed diagnosis and misdiagnosis .

This paper was designed to study the effects of cellulose, pectin and ag-ar at 10% level on the concentration of serum total cholesterol (TC), trigly-ceride (TG) and high density lipoprotein cholesterol (HDL-C) in rats. Male adult Wistar rats were randomly divided into 5 groups and fed on basal diet, hypercholesterolemic diet (control diet) and 3 test diets (contain 10% cellulose, pectin or agar respectively) for 6 weeks. The results showed that all three fibrous diets were significantly lowered serum TC (P

Objective To explore the relationship between self-efficacy and health behavior among stroke survivors.Methods Self-efficacy for Managing Chronic Disease 6-item Scale and Health Promoting Lifestyle Profile II were used to measure 96 stroke survivors’ self-efficacy and health behaviors.Pearson correlation analysis was used for the analysis of correlation.Results The mean score of overall health behaviors was (2.41±0.33).Pearson’s correlation coefficient between self-efficacy and health behavior was 0.36 (P<0.05).Conclusions The health behaviors of stroke survivors were at the intermediate or lower level and their self-efficacy was at the intermediate level.In order to help stroke survivors to improve their health behaviors,self-efficacy should be enhanced by specific health education.

Objective To explore the influence of family function on health behaviors of stroke patients and the mediating effects of depression.Methods From September 2011 to April 2012,101 stroke survivors from a certain hospital in Guangzhou were investigated with Health Promoting Lifestyle Profile Ⅱ (HPLP Ⅱ),Family Assessment Device (FAD) and Self-Rating Depression Scale (SDS).Results The mean score of overall health behavior was (2.27±0.36).The mean score of overall family function was (2.18±0.25).The score of overall depression was (37.39±7.94),which was higher than normal people.And the incidence of the stroke survivors' depression state was 29.5％.There was a correlation between family function,depression and health behavior.The depression totally mediated the influence of problem solving on health behaviors.The depression partially mediated the influence of communication,affective responsiveness,behavior control on health behaviors,and their mediating effects were 32.1％,37.4％,26.1％.Conclusions The promotion of family function and the reducing of depression can be regarded as the in-tervention to improve their health behavior.

Background and purpose:Hepatitis B virus X protein (HBx) and hypoxia inducible factor-1α(HIF-1α) play key roles in hepatocarcinogenesis and the development of hepatocellular carcinoma. Positive correlation on the expression of these 2 proteins in hepatocellular carcinoma tissues has been found, whereas the underlying mechanisms have not been fully elucidated. This study focused on the role of HBx in regulating HIF-1α and the underlying mechanisms in hepatocellular carcinoma cells. Methods:The expression plasmids were transfected into Huh7 cells with LipofectemineTM 2000. Western blot analysis was applied to detect the expressions of HIF-1αand HIF-1β protein. The transcriptional activity of HIF-1α was detected by the commercial analysis kits. The mRNA levels of HIF-1αand its target genes, including vascular endothelial growth factor (VEGF) and multi-drug resistance gene 1 (MDR1), were detected by quantitative real-time PCR (qRT-PCR). Immunoprecipitation analysis was applied to detect the interaction of HIF-1α, HBx and protein von Hippel-Lindau (pVHL). Results:Huh7 cells transfected with HBx plasmid led to sharp increase of HIF-1αprotein and transcriptional activity, as well as the mRNA of VEGF and MDR1 (P<0.05). However, the mRNA level of HIF-1αwas not obviously changed after HBx transfection (P>0.05). Meanwhile, HBx also signiifcantly impaired the function of pVHL in mediating the degradation of HIF-1αby ubiquitin hydrolase. This finding was further confirmed by the immunoprecipitation analysis, which showed that HBx could directly bind to pVHL, but not to HIF-1α. Conclusion:HBx may inhibit the inter-activation between pVHL and HIF-1αthrough directly binding to pVHL, and thus enhance the stability and transcriptional activity of HIF-1α.

Objective To investigate the changes of urine conductivity in common kidney diseases and the relationship between urine conductivity and cystatin C.Methods A total of 723 patients in the First Affiliated Hospital of Dalian Medical University were selected in this study and divided into IgA nephropathy group(81 patients),diabetic nephropathy group(104 patients),ne-phritic syndrome group(130 patients),multiple myeloma group(89 patients),chronic glomerulonephritis group(1 1 5 patients),renal calculus group(101 patients)and chronic renal failure group(103 patients).Other 205 healthy persons were recruited into control group.The urine samples were analyzed by Sysmex automatic urinary sediment analyzer UF 1000i to determine the urine conductivi-ty,while the serum samples were analyzed by Hitachi automatic biochemical analyzer 7600 to determine the cystatin C.All the re-sults were statistically analyzed by SPSS1 9.0 software.Results The urine conductivity in kidney patients was significant lower than that of healthy people (P <0.05).There was a significant negative correlation between urine conductivity and the cystatin C in the kidney patients(P <0.05).Conclusion The urine conductivity might serve as an important index in evaluating the kidney func-tion and urine concentration.

Objective To analyze the quality of life and its influence factors in patients with peptic ulcer.Methods The SF-36 was used to survery 120 patients with peptic ulcer.The QOL scores at different levels of some factors including gender,age,na-tionality,occupation,marriage,medical forms,economic,cultural level,treatment method and clinical type were compared by meth-ods of two sample t-test and analysis of variance.Results Peptic ulcer patients′scores in the domain of RE,PF,RP,BP,GH,VT, SF,RE,PCS and MCS are lower than that of the normal,exception of the domain of MH(P <0.05).The differences were statisti-cally significant.Six factors can be influenced some domain or total score of the scale(P <0.05)and the differences were statistical-ly significant.Conclusion Find out the influencing factors on peptic ulcer patients′quality of life can be provided the basis for clini-cal treatments.

AIM: To observe the anti-inflammatory effect of CQMUH-011 and to explore its mechanism.METHODS: Three kinds of animal models, mouse ear swelling induced by xylene, rat granuloma induced by cotton ball and rat rheumatoid arthritis induced by Freund's complete adjuvant, were established to study the anti-inflammatory effect of CQMUH-011.The ear swelling degree, dry weight of cotton ball granuloma, arthritis index, paw swelling and ankle joint pathological changes were measured to reflect the severity of inflammation.The anti-inflammatory mechanisms of CQMUH-011 were investigated by detecting the serum levels of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) by ELISA.Malondialdehyde (MDA), myeloperoxidase (MPO) and nitric oxide (NO) were determined by corresponding kits.RESULTS: Treatment with CQMUH-011 significantly decreased TNF-α, IL-6 and NO concentrations, MDA contents and MPO activity in the serum.Meanwhile, Ear swelling degree, dry weight of cotton ball granuloma, arthritis index, paw swelling and ankle joint pathological damage were attenuated.CONCLUSION: CQMUH-011 has an anti-inflammatory effect, which may be related to inhibiting the production of inflammatory factors and attenuating lipid peroxidation.