The feasibility of medical college and university libraries to serve grass-root physicians was analyzed and the new approaches for medical college and university libraries to provide characteristic information service for grass-root physicians were studied according to the investigation of the utilization of information resources, types of information needs, satisfaction degree of information service, access methods of information, and hopes for access to medical information in grass-root physicians.

Objective To explore the diagnostic value of multi-slice spiral CT MIP-MPR image in regional lymph node metastasis of colonic carcinoma.Methods CT findings of 33 cases with pathological confirmed colonic carcinoma were analyzed retrospectively . All patients were underwent three-phase 256-slice CT dynamic enhancement scanning.All data were processed in workstation,to obtain routine MPR and MIP-MPR image.The regional lymph nodes of colon cancer were counted,and divided into 3 groups accord-ing to the short diameter of lymph node:group I (3.0-5.0 mm),group Ⅱ(5.1-10 mm),and group Ⅲ(10.1 mm up).The diag-nostic sensitivity of CT MPR and MIP-MPR image in checking out lymph nodes of different groups was compared by paired test.Re-sults The average value(x ±s )of lymph node number of three groups with routine MPR and MIP-MPR image were:group I, 3.66±1.99,6.09±2.44 (t=1 1.6,P <0.05);group Ⅱ,3.72±1.79,4.03±1.89 (t=3.73,P <0.05);group Ⅲ,1.21±1.31, 1.27±1.46(t=1.44,P >0.05).There were statistical differences in group I and group Ⅱ.Sensitivity of MIP-MPR image in chec-king out regional small and middle lymph nodes of colonic carcinoma was higher than routine MPR image.Conclusion Multi-slice spiral CT MIP-MPR image is a good way to checking out regional small and middle lymph nodes of colonic carcinoma.

Objective To investigate effects of octreotide combined with aspirin on the growth and metastasis of gastric cancer in vivo. Methods SGC-7901, human gastric cancer cell line, was implanted orthotopically in the stomach of nude mice. Octreotide or aspirin alone and the combination of the two drugs were administrated for 8 weeks to investigate the effects of them on tumor growth and metastasis. Results At necropsy, xenografts in situ were found in all stomachs of nude mice except two nude mice in the combined group. The mean volume and weight of tumors in nude mice treated with octreotide or aspirin were significantly lower than those of control group. The inhibitory rate for tumors was 60.6% in octreotide group, 39.3% in aspirin group and 85.6% in the combined group with octreotide and aspirin respectively. The metastatic rates of gastric adenocarcinoma were markedly lower in any of treatment group than that in control group. No severe side action was observed in all of treatment group. Conclusion Octreotide combined with aspirin greatly enhanced the anti-growth effect on gastric cancer in vivo when compared to using either of them and inhibited the metastasis of gastric adenocarcinoma in nude mice.

Objective To study MSCT findings and clinic value of hepatic abscesses on enhanced double-phase and(or)three-phase(arterial and protal venous phase and delayed phase),especially during arterial phase. Methods The MSCT imaging features of 20 cases with hepatic abscesses proved by operation or biopsy were evaluated on enhanced double-phase and(or) three-phase scan during arterial and portal venous phase and delayed phase ,especially during arterial phase.Results (1)The imaging features of aeterial phase were that the hepatic tissues around the lesions showed strong enhancement,but the lesions showed slight enhancement or not or peripheral enhancement. (2)The imaging features of protal venous phase were:a.circular target sign; b.circular sign; c.flower valve sign; d.multiloculated sign. (3)The imaging features of delayed phase were that the masses changed smaller or not and the belt disappeared or got vague. Conclusion The double-phase or three phase MSCT scan fully reflects the pathologic changes of hepatic abcesses and is very important to early diagnosis and differential diagnosis.

Objective To investigate the visual acuity improvement and related factors for myopia after femtosecond flap excimer laser surgery. Methods 72 myopic patients (129 eyes) in our hospital between January 2010 and December 2013 were chosen. The patients all received femtosecond flap excimer laser surgery. The visual acuity improvement after surgery was observed, and the influencing factors were analyzed. Results The distance vision (VA), near vision (NVA) and best corrected visual acuity (BCVA) significantly increased before surgery (P<0.05). Logistic regression analysis showed that preoperative BCVA, NVA, axial length, cutting ratio, axial length and time of near vision were the affecting factors of visual acuity (P<0.05). Conclusion Femtosecond flap excimer laser surgery for myopia has significant effect , but the preoperative visual acuity was affected by BCVA , NVA, cutting ratio, axial length and time of near vision.

OBJECTIVE To investigate the effect of the Qingfei decoction in the acute upper respiratory tract infections.METHODS From Jan 2004 to Sep 2006,there were 68 cases with acute upper respiratory tract infections treated with chinese medicines.The patients were divided into 2 groups:36 cases were treated with Qingfei decoction,32 cases were treated with Shuanghuanglian granules as a control.Both of the groups were treated for 5 days,the clinical symptoms such as: fever,cough and sore throat were used to evaluate the efficacy of both groups.RESULTS Both of the groups were effective;the effective rate was 88.9% and 75.0% in the Qingfei group and in the control group,respectively.There was significant difference between 2 groups(P

Objective To present 8 cases of retroperitoneal laparoscopic pyelolithotomy or ureterolithotomy for the treatment of pelvis and ureteral stone. Methods 8 cases of pelvis or ureteral stone have been treated by retroperitoneal laparoscopic pyelolithotomy or ureterolithotomy. The size of the calculi was 0.8～2.4 cm with the incarceration time of 3 months～2 years.Details of the procedure were presented. Results Success has been achieved in all the patients and no complication has been observed during the 3～6 months of follow up. Conclusions Retroperitoneal laparoscopic pyelolithotomy or ureterolithotomy for the treatment of pelvis or ureteral stone is simple ,safe,less traumatic and effective.

Objective To investigate the stabilization of betaine on the enzyme activity of ALT in human serum at different temperatures, and to evaluate the matrix effects of human serum with betaine among different analytical systems. Methods The enzyme activity of ALT in human serums with l mol/L betaine stored at 37 ℃, 25 ℃, 4 ℃ and - 20 ℃ respectively were measured. The matrix effect experiment was carried out according to the procedures specified in Clinical and Laboratory Standards Institute (CLSI)document EP14-A. Four analytical systems were the candidates for evaluation, and Roche Diagnostics (Roche)-Hitachi analytical system served as the reference system. The enzyme activity of ALT of 40 patient specimens and 3-level specimens ( high, medium and low) with 1 mol/L betaine were measured using the evaluated methods and the reference method respectively. The linear regression analysis was performed to compare the mean y value of 3-level specimens with the statistically defined limits ( the two-tailed 95% prediction interval) derived from 40 patient specimen data. Results The enzyme activity of ALT could be stabilized with betaine in serum, which remained to be 99% for 48 h at 37 ℃, 98% for4 d at 25 ℃, 97%for 4 weeks at 4 ℃, and 99% for 4 months at - 20 ℃. The enzyme activity of control serum decreased to 42%, 86%, 71%, 79% respectively. The mean y values of three specimens with the betaine were within the 95% prediction interval of the estimated mean y values, which suggested no obvious matrix effects in serum. Conclusion Betaine can be used in the development of enzyme ALT calibrator as its stabilizer.

Objective To investigate therapeutic mechanism of radiation therapy associated with Doeetaxel on human uterine cervi-cal carcinoma. Method Hela cells were incubated with 0. 5 μmol/L, 1.01 μmol/Docetaxel for 24 hours, and were radiated with 0, 2, 4, 6, 8Gy X ray. The proliferation activity was detected with MTT method. Apoptosis was evaluated with TUNEL method. Expression of caspase-3 protein and the degradation fragment was measured with Western blot. Result Inhibitory effect of radiation therapy associated Docetaxel on Hela cells was significantly stronger than that of radiation group. Treated with 2 Gy and 6MV X ray radiation, inhibitory rate of Hela cells increased with the increment of associated Docetaxel doses (P<0.05). Inhibitory rate of Hela cells in radiation associated Docetaxel group was significantly increased compared with radiation group. Apoptosis index in radiation associated Docetaxel group 48 hours after treatment was significantly increased compared with radiation group or Docetaxel group with TUNEL method (P<0.05). Western blot results indies-ted that caspase-3 protein was degraded and small active fragments were produced. Conclusion The reason that Docetaxel enhanced Hela cell radiation sensitivity may probably he correlated to easpase-3 protein activation.

Objective To investigate the effects, and possible mechanism of action, of vasoactive intestinal peptide (VIP) on acute lung injury in a rodent model of endotoxic shock. Method Endotoxic shock was induced in Sprague-Dawley (SD) rats by intravenous injection of lipopolysacchaiide (LPS) at 10 mg/kg. Three groups (each group with 10 rats), were given injections of either normal saline (Control), LPS 10 mg/kg (LPS group), or LPS 10 mg/kg + VIP 5nmol (VIP). Samples were collected 6 hours after injection. Indices of lung injury including lung wet/dry weight ratio, protein concentration and neutrophil count in bronchoalveolar lavage fluid( BALF) were derived. Assays of TNF-α,IL-1β, IL-10 in serum and BALF were performed using ELISA. Light and electron microscopy were used to detect histopathological changes in lung tissues. Results The lung wet/dry weight ratio, protein concentration and neutrophil count in BALF were significantly raised in the LPS group compared to the Control group (P ＜ 0.05). These indices were significantly lowered in the VIP group compared to the LPS group, though not to the level of the control group. Concentrations of TNF-α, IL-1β, IL-10 in serum and BLAF also increased in the LPS group compared to the control group (P ＜ 0.0S). Levels of TNF-α and IL-1β were significantly lowered in the VTP group compared to the LPS group (P ＜ 0.05), while levels of IL-10 was significantly raised ( P ＜ 0.05). Histopathological changes due to lung injury were not as severe in the VIP group compared to the LPS group. CondusKms VIP plays a protective role during acute lung injury induced by endotoxic shock in rats. Its mechanism of action may be related to down-regulation of proinflammatory cytokines and up-regulation of anti inflammatory cytokines.