The existing literature on fingerprint chromatography at home and abroad and the quality control of biochemical injection with multiple components were reviewed in this article.Combined with the laboratory research, It is proposed that the strategy for HPLC specific chromatography of biochemical injection with multiple components in order to provide the basis for effectively promoting the establishment and development of HPLC specific chromatography of biochemical injection with multiple components.

Objective To evaluate the curative effects of venlafaxine in patients with depressive disorder between therapeutic modes of inpatient and outpatient. Methods Seventy-two patients with depressive disorder including 36 inpatients and 36 outpatients were measured respectively with HAMD24 after treatment for 1 week, 2 weeks, 4 weeks and 8 weeks, and then the curative effects were compared between two groups. Results It was found that all patients in the inpatient group completed the observation and two patients in the outpatient group didn’t complete the observation. The HAMD24 score was significantly decreased in the inpatient group than that of the outpatient group from the second week to the end of the eighth week (P<0.05). The total effective rates were 80.6% for inpatient group and 64.7% for outpatient group, no significant difference between them. Conclusion Venlafaxine shows a higher therapeutic value and outcome in the therapeutic mode of inpatient.

Objective To investigate the self management behaviors and analyze the related factors in patients with coronary heart disease(CHD) in order to provide the evidence for strategy making on health education and secondary prevention of CHD.Methods Totally 209 patients with verified CHD who were from 3 grade-A hospitals in Chongqing from October 2007 to January 2008 were investigated with Coronary Heart Disease Self Management Scale(including three sub-scales: daily management,medical management and emotional management).Their general information and the disease related information were also collected.Results The score of self management in the cohort of patients was(52.69?18.47).The multi-variable stepwise regression showed there were statistically significant correlation between the daily life management and working status;between the medical management and education background,hypertension,classification of CHD and duration of CHD;between the emotional management and disease perception,economic status(P

Aim To explore whether total flavonoids of Verbena officinalis L(TFV)can induce apoptosis of HepG-2 cells through targeting topoisomerase Ⅱ.Methods HepG-2 cells were cultured with TFV at 200,100,50 mg·L-1.Cell apoptosis was evaluated by TUNEL-DAPI double staining.TopⅡ activity was detected by supercoiled pBR322 DNA relaxation assay.The levels of the mRNA of TOP Ⅱα,TOP Ⅱβ were analyzed by real time PCR.Expression of Bcl-2,Bax,TOP Ⅱα,TOP Ⅱβ and caspase-3 was analyzed by Western blot.

The analysis of 3 Teaching Basic Skills Contests showed that the Teaching Basic Skills contests could boost young teachers’teaching enthusiasm,urge them to improve their own quality,enhance their training on basic skills to improve their teaching abilities.

Objective To detect the content of(1-3)-β-D-dextran(BG)and CD4T lymphocytes in order to early diagnose invasive pulmonary fungal infection(IPFI)in AIDS patients.Methods According to the qualified lower respiratory tract fungal culture re-sults,153 patients with pulmonary infection symptoms and definitely diagnosed AIDS were divided into the positive IPFI group(63 cases)and the negative IPFI group(90 cases);then plasma BG and CD4T lymphocytes were simultaneously detected in the positive IPFI group,negative IPFI group and 50 healthy controls.The same index was performed the pairwise comparison in each group and the statistical analysis was conducted.Results The mean values of BG and CD4T lymphocytes in the positive IPFI group were (56.30±15.38)pg/mL and(56±41)/μL;which in the negative IPFI group were(21.32±14.26)pg/mL and(200±53)/μL;which in the healthy control group were(2.89±1.55)pg/mL and(480±89)/μL.The pairwise comparison of BG and CD4T lymphocytes showed the statistical differences among the three groups by the t-test(P <0.01).Conclusion The combined detection of BG and CD4 lymphocyte has the value in early diagnosis of IPFI)on AIDS apparent infection.

Objective:To investigate the role of TSLP and NF-κB in the pathogenesis of adenomyosis.Methods:Immunohisto-chemistry was employed to detect the expression of TSLP and NF-κB p65 in the endometrial glandular cell and stromal cell of 35 adeno-myosis patients and 20 hysteromyoma patients as control , and analyze the correlation of the two proteins.The concentration of TSLP was measured by specific ELISA in the serum of healthy subjects besides of the patients with adenomyosis and hysteromyoma .Results:TSLP and NF-κB p65 were both positively expressd in the glandular cells and stromal cells of ectopic and eutopic endometrium of patients with adenomyosis , their expression was significantly higher than that of the control group ( P<0.01 ) , and the expression of TSLP and NF-κB p65 in ectopic endometrium was significantly higher than that in eutopic endometrium ( P<0.01 ).There was positive correlation between TSLP and NF-κB p65 in ectopic endometrium.The concentration of TSLP was higher in the serum of patients with adenomyosis.Conclusion:The TSLP is highly expressed in endometrial glandular and stromal cells of patients with adenomyosis , and there is higher level of TSLP in serum , and there is correlation between the expression of TSLP and NF-κB in ectopic endometrium.

0.05).Conclusion:The abnormal expression of HLA-G protein in eutopic and ectopic endometrium tissues may play a key role in the pathogenesis of adenomyosis.

Objective To evaluate the changes of thyroid function during pregnancy in the second and third trimester in the regions with or without iodine deficiency. Methods The serum TSH, FT 3, FT 4 and TT 4 levels of 90 pregnant women (30 from iodine deficient area as study group, 60 from iodine sufficient area as control group) and 30 non pregnant women were assayed by high sensitive immunoradiometric assay (IRMA) or radioimmunoassay (RIA), and urine iodine level of pregnant women before delivery was also examined. Results (1) The serum TSH level in pregnant women in the second and third trimester was significantly higher than that of non pregnant women. The serum free thyroid hormone levels of pregnant women were significantly lower than that of non pregnant women, especially in the third trimester. (2) The serum TSH level of pregnant women from iodine deficient area in the second trimester and before delivery was significantly higher than that of pregnant women from iodine sufficient area (P

Objective To investigate the application value of real clinical case centered situation simulation examination model in clinical medicine. Methods Two classes were randomly selected from 2008 grade clinical medicine majors, respectively as test group(n=158) and control group(n=156). Final examination of test group includes usual performance, case-oriented situation simulation examination (COSSE) and final written examination. Traditional methods were used in control group. The same paper was used in final written examination of the two groups. Scores of written examination and question-naires were used to evaluate the COSSE model. Data were processed using SPSS 16.0 statistical software;t and chi-square tests were used to analyze the data;P<0.05 signifies that the difference is statistically sig-nificant. Results Scores of final examination in test group were higher than those in control group(P<0.001). Times of independent completion of history taking, physical examinations and medical record writing were more in test group than in control group(P=0.002,0.017,0.048). Conclusions Contents and methods of COSSE is conducive to improving student's professional comprehensive ability.