Objective:To develop a method for the determination of natural benzoic acid in Mesona chinensis Benth. with different production places and storage time. Methods:HPLC was adopted, the column was an Agilent ZORBAX SB-C18 (250 mm × 4. 6 mm, 5 μm) column, the mobile phase was methanol-0. 02 mol·L-1 ammonium acetate (5∶95) at the flow rate of 1. 0 ml·min-1 , the col-umn temperature was 35℃, the detection wavelength was 230 nm, and the injection vocume was 10μl. Results:Benzoic acid showed good linearity over the concentration range of 0. 1-10. 0 μg·ml-1(r=0. 999 9). The average recovery was 99. 59%(RSD=1. 57%, n=9). Natural benzoic acid in powered Mesona chinensis Benth. was different because of various production places. The maximum content of natural benzoic acid in Mesona chinensis Benth. was 141. 8 mg·kg-1, while the minimum content was 73. 8 mg·kg-1. The content of natural benzoic acid in Mesona chinensis Benth. stored for two years was declined obviously. Conclusion: The method with simple operation is accurate and reproducible. It can provide a basis for the further study of Mesona chinensis Benth. resources, and provide a reference for the content determination of natural benzoic acid in other plants.

Objective To investigate the influence of postoperative cognitive dysfunction (POCD) to clinical outcome in adult patients and the risk factor. Methods 122 patients after operation on chest were surveyed with Wechsler Adult Intelligence Scale-Revised China (WAIS-RC) within 3 d before operation and on the 7th day after operation. Their pulmonary functions were measured before discharged. Their time on bed, total time in hospital, time after operation in hospital, time in intensive care unit and spending for therapy were investigated. Results 45 patients were finally diagnosed as POCD (POCD group) 7 d after operation. Before operation, the age, the body weight index, the smoking index and the number of risk factor of angiosclerosis in the POCD group were all higher than those whose cognitive function were normal (normal group) (P<0.05). The sores of insight, count, digit scope, vocabulary, figure collocation, figure patch of WAIS-RC in POCD group were obviously lower than those in normal group (P<0.05). Every index of pulmonary function in POCD group was lower than those in normal group(P<0.05). The time keeping on bed, total time in hospital, time after operation in hospital, time in intensive care unit and spending for therapy in POCD group all exceeded those in normal group (P<0.05).Conclusion The adult patients with POCD may spend more time and money for therapy in hospital, and their pulmonary functions may be recovered poorly.

Objective We detected the expression of the low risk HPV6/11 and the high risk HPV16/18 in cervical lesions in order to investigate the correlativity,mechanism and clinical values between them.Methods We chosed 150 cases of cervical disease tissues that included chronic cervicitis (30 cases).cervieal condyloma without atypia(30 cases),CIN Ⅰ(30 cases),CIN Ⅲ(30 cases)and cervical squamous cell carcinoma (30 cases).And all of them were constructed into tissue microarrays(TMA)that include 300 spots.Then,the expression of HPV6/11 and HPV16/18 were determined by in situ hybridization.Results The positive expression rates of HPV6/11 were 13.33%,90%,33.33%,0 and 0 respectively in chronic cervicitis,cervical condyloma without atypia,CIN Ⅰ,CIN Ⅲ and cervical squamous cell carcinoma.Condyloma showed significantly higher HPV6/11 infection rates than the other groups(P＜0.001).And there were obvious dliference between CIN Ⅰ and CIN Ⅲ & carcinoma (P=0.001.＜0.05).The spearman correlation analysis suggested that the expression of HPV6/11 in the five cervical deseases was negative correlation (rs=-0.037,P＜0.001).The positive rates of HPV16/18 were 0,6.67%,10%.56.67%.76.67% respectively in chronic cervicitis,cervical condyloma without atypia,CIN Ⅰ,CIN Ⅲ and cervical squamous cell carcinoma.Comparing CIN Ⅲ and carcinoma with the other groups,the positive rates were obvious defrerence between them (P＜0.001).But there was not significant defference between CIN Ⅲ and carcinoma (P=0.10).The spearman correlation analysis suggested that the expression of HPV16/18 in the five cervical deseases was positive correlation (rs=0.628,P＜0.001).Conclusion Low risk HPV6/11 is a main reason for the cervical condyloma and CIN Ⅰ.High risk HPV16/18 is closely related with CIN Ⅲ and carsinoma.and also a main reason for CIN Ⅲ and carcinoma.Detecting and differentiating HPV type will help for the diagnosis and monitor of cervical lesions,especially,it will be very important to prophylaxis,early diagnosis and early therapy of cervical carcinoma.

Objective To raise the vigilance for pregnancy complicated with acute abdominal diseases and to reduce the maternal mortality rate. Methods A retrospective study was done. Results (1) The maternal mortality of pregnancy complicated with acute abdominal diseases was 1 31/10 5 in the period of 1989～1993, which was significantly higher than that in the period of 1994～1998 (0 71/10 5). (2) The main cause of maternal deaths was misdiagnosis and the rate of misdiagnosis was 72 7%.(3) Among the acute abdominal diseases, pregnancy complicated with pancreatitis ranked the first that accounted for 54 6%. Conclusions For the purpose of reducing the mortality rate of pregnancy complicated with acute abdominal diseases, the correct diagnosis and proper treatment especially for those with acute pancreatitis are important.

Mesenchymal stem cells(MSCs), which can be introduced in brain tissue by the strategies of brain parenchyma, blood and neurolymph transplantation, to repair tissue injury and promote functional recovery of neural system, have been considered as the ideal seed cells for the treatment of central nervous system diseases. However, as the special structure exists in the blood-brain barrier, study on correlation between MSCs and blood-brain barrier permeability will make it possible for more MSCs to pass the barrier to perform the treatment. This paper gives a review on the latest research development in the field.

Objective To explore the value of maximun P wave duration (P max ) and P wave dispersion (P d),that is,the difference between the maximum and minimun P wave duration in patients with idiopathic paroxysmal atrial fibrillation.Methods P max and P d were measured from 12 lead surface electrocardiogram among 20 patients with a history of idiopathic paroxysmal atrial fibrillation and 20 age matched healthy control subjects.Results P max and P d were found to be significantly higher in patients with a history of idiopathic paroxysmal atrial fibrillation than in the control subjects(P

According to the characteristics of low cost, high performance, high integration and long battery life of wearable medical devices, the mainstream low-power microcontroller(MCU) series were compared, and came to the conclusion that the MCU series based on ARM Cortex-M0+ architecture were suitable for the development of wearable medical devices. In aspects of power consumption, operational performance, integrated peripherals and cost, the MCU series based on Cortex-M0+ architecture of primary semiconductor companies were compared, aimed at providing the guides of MCU selection for wearable medical devices.
Durable Medical Equipment ; Electric Power Supplies ; Monitoring, Ambulatory ; instrumentation

To explore the efficacy of using gonadotropin-releasing hormone agonist (GnRH-a) with different cycles after conservative laparoscopic surgery in the treatment of endometriosis.Research was conducted on 108 patients with endometriosis Ⅲ or Ⅳ diagnosed by conservative laparoscopy from January 2010 to June 2011.Among them,57 patients (group A) were managed with GnRH-a postoperatively for 3 months and the others (group B) 6 months.Clinical efficacy,relapse rate and pregnancy rate after withdrawal were compared between two groups.The clinical efficacy rate of groups A and B were 73.7％ and 90.2％ respectively.And there were significant statistical differences between them.However,the relapse rate of groups A and B were 14.0％ and 5.9％ and the pregnancy rate of two groups 45.0％ and 4/12 respectively.And there were no statistical differences.A 6-month regimen of GnRH-a can reduce and delay the recurrence of endometriosis and improve the clinical efficacy.A short-term dosing of GnRH-a is indicated for those patients with fertility requirements.

AIM: To study the effects of IL-6 and IL-1? on the blood polymorphonuclear-neutrophils(PMN) apoptosis postburn. METHODS: Wistar rats inflicted by 30% total body surface area (TBSA) Ⅲ degree scalding were employed as the model. PMN were isolated by density gradient centrifugation using Percoll-hypaque and labeled with TdT-mediated and dUTP nick end labeling (TUNEL) and analyzed by flow cytometric analysis. The intracellular caspase-3 activation and the serum levels of IL-6 and IL-1? were analyzed by fluorometric immunosorbent enzyme assay and enzyme-linked immunosorbent assay, respectively. RESULTS: The serum IL-6 levels (?g/L) in groups of 3, 6, 12, 24 and 48 h postburn (9 14?1 16, 12 49?1 14, 3 01?0 75, 1 41?0 28 and 1 56?0 43 in turn) and IL-1? (ng/L) in groups of 3, 6, 12 h postburn (90 08?8 39, 320 93?14 48 and 47 84?5 19) were much higher than IL-6 (0 24?0 07) and IL-1? (27 65?4 86) in control group ( P

Objective To analyze the pregnancy outcomes of 204 cases of multiple pregnancies following early transvaginal fetal reduction and sum up the experiences of peri-operation nursing care. Methods A total number of 204 twin and triplet pregnancies under treatment in the reproductive medical center from Jan. 2009 to Dec. 2012 were involved in the study. All patients underwent transvaginal multifetal pregnancy reduction during pregnancy of 7 to 8 weeks . The pregnancy outcomes were analyzed , and the nursing measures including preoperative preparation , intraoperative cooperation and postoperative nursing were analyzed . Result The successful rate of multifetal reduction was 100%, and the rate for taking the infants home reached 77.0% ~ 92.3%. Conclusion The outcomes of multifetal reduction for the twin or triplet pregnancy are good . Such nursing measures as perioperative mental care , intraoperative cooperation and postoperative follow-up are key to the alleviation of mental pressure during operation , raise of the success rate of fetal reduction and prevention of postoperative complications.