Objective To observe the effect of crawling exercise and bilateral electrical stimulation on the upper limb motor after stroke. Methods 60 stroke patients were divided into experimental group (n=30) and control group (n=30). Both groups received routine therapy. The control group received unilateral electrical stimulation on the upper limbs, and the experimental group received electrical stimulation on bilateral upper limbs and crawling exercise. All the patients were assessed with the modified Ashworth scale (MAS), the Fugl-Meyer assessment (FMA) and the modified Barthel index (MBI) before and after treatment. Results After 8 weeks of treatment, the scores of MAS, FMA and MBI improved in both groups (P<0.05), and improved more in the experimental group than in the control group (P<0.05). Conclusion The crawling exercise and bilateral electrical stimulation can improve the motor of upper limbs and activities of daily living more effectively than the unilateral electrical stimulation after stroke.

Objective:To analyze 3 123 cases of medical device adverse events(MDAE)and provide some suggestions for the MDAE monitoring.Method:By the retrospective case study,3 123 MDAE reports were analyzed in Hunan Province in 2007.Result:The reports mainly came from medical institutions and other drug-use units.Intrauterine devices (IUD),medical suture thread and orthopedic implants caused a higher proportion of adverse events.The quantities of MDAE reports were imbalanced in different regions.Conclusion:The MDAE monitoring should be promoted by propaganda and training in order to ensure the safe use of medical devices in public.

Objective To detect the content of(1-3)-β-D-dextran(BG)and CD4T lymphocytes in order to early diagnose invasive pulmonary fungal infection(IPFI)in AIDS patients.Methods According to the qualified lower respiratory tract fungal culture re-sults,153 patients with pulmonary infection symptoms and definitely diagnosed AIDS were divided into the positive IPFI group(63 cases)and the negative IPFI group(90 cases);then plasma BG and CD4T lymphocytes were simultaneously detected in the positive IPFI group,negative IPFI group and 50 healthy controls.The same index was performed the pairwise comparison in each group and the statistical analysis was conducted.Results The mean values of BG and CD4T lymphocytes in the positive IPFI group were (56.30±15.38)pg/mL and(56±41)/μL;which in the negative IPFI group were(21.32±14.26)pg/mL and(200±53)/μL;which in the healthy control group were(2.89±1.55)pg/mL and(480±89)/μL.The pairwise comparison of BG and CD4T lymphocytes showed the statistical differences among the three groups by the t-test(P <0.01).Conclusion The combined detection of BG and CD4 lymphocyte has the value in early diagnosis of IPFI)on AIDS apparent infection.

Objective To clone and express translocation intimin receptor(Tir)of enterohemorrhagic Escherichia coli(EHEC)O157:H7,and to analyze the effect of different routes on the induction of immunity to the recombinant protein.Methods The tir eucoding genes were amplified from EHEC O157:H7 strain guangzhou 246 genome,and genes were cloned into the vector pET-30a(+).The pET-30a(+)tir recombinant was transformed into E.coli BL21.and expression was induced bv IPTG.The expressed product was analyzed by SDS-PAGE and purified by Ni-IDA affinity chromatography.The immunized mice sera and fecal against the recombinant protein was detected.Resuits The length of the tir is 1677 bp,with the initiation codon ATG and the termination codon TAA.Double enzyme digestion and DNA sequencing confirmed that the recombinant expression plasmid pET-30a(+)-tir was constructed.The recombinant protein was expressed in Escherichia coli expression system,and was purified by Ni-IDA affinity chromatography.The mice were able to produce a high serum IgG antibody titer after both subeutaneous and intranasal immunizations.Meanwhile,the intranasal immunization induced serum and fecal IgA antibody titer was significantly higher than that of the subcutaneous immunization group.Conclusion Tir molecule is potential vaccine candidate for preventing EHEC disease.

Objective To explore appropriate organization and financing models of urban employees supplementary medical insurance under the conditions of the market economy. Methods Employee surveys on willingness to participate in, willingness to pay for, and actual enrolment in supplementary medical insurance were conducted in Shanghai, Wenzhou and Chengdu and nationally different operational models were compared so as to analyze prospectively coverage and operational models of feasible supplementary medical insurance options. Results Characteristics of the employees needs for various supplementary medical insurance options were garnered and appropriate coverage and operational models were put forward. Conclusion It is suggested that the role of the government in the development of supplementary medical insurance be clearly defined, the development of supplementary medical insurance be promoted through policy support and guidance, and the supervision of supplementary medical insurance be strengthened.

Based on the intermolecular interaction, a molecularly imprinted polymer electrochemical sensor with specific identification of cavity on gold electrode surface was proposed with o-aminophenol as functional monomers and morin as template molecule. The performance and effect of MIP were investigated by cyclic voltammetry ( CV) and differential pulse voltammetry ( DPV) . Factors affecting the properties of sensor, such as polymeric membrane ratio, scanning cycles, elution time and adsorption time were investigated. Substances which had a similar structure to morin was used to compared the selective response. The result showed that the sensor had a good selectivity to morin. Under the optimized experiment conditions, the current response of the imprinted sensor was linear to the concentration of morin in the range of 0 . 05-1 . 7 μmol/L with linear equation as follows: I(μA)= 1. 0800lgc(mol/L)+ 9. 3599(R=0. 9934), and the detection limit of 0. 1 μmol/L. The sensor was used to determine the content of morin in black tea samples, and the recoveries of standard addition were between 104 . 0% and 108 . 0%.

OBJECTIVE: To evaluate the influence of fewer courses and prolonged intervals of chemotherapy on survival rate of advanced non small cell lung cancer (NSCLC) patients treated by sequential chemo-radiation therapy combined with traditional Chinese medicine (TCM). METHODS: From Jan. 2000 to Dec. 2001, 54 untreated advanced NSCLC patients (2 stage IIIa, 18 stage IIIb, 34 stage IV) were treated by sequential chemo-radiation therapy combined with TCM. The courses of chemotherapy were reduced and the intervals of chemotherapy were longer than that of the standard regimen. The efficacy and survival rate were documented and the prognostic factors were analyzed. RESULTS: Complete remission (CR) was observed in 1 case and partial remission (PR) in 20 cases. The overall objective response rate was 40.4%. Median survival was 15.3 months, 1-, 2- and 3-year survival rate were 53.7%, 28.9% and 9.6% respectively. The median survival of stage III and IV were 21.8 months and 12.5 months respectively. The 1-, 2-, and 3-year survival rates of stage III were 65.0%, 49.5%, 24.7% and that of stage IV were 47.0%, 23.3%, 0%, respectively. The quality of life was improved in most of the patients. Cox's proportional hazards regression showed that improved quality of life and treatment of TCM were the significant prognostic factors of overall survival. CONCLUSION: Chemotherapy and radiotherapy combined with TCM is beneficial to extending the interval of chemotherapy, improving the quality of life, and increasing the survival rate of advanced NSCLC patients.

Objective To evaluate the effect of sodium nitroprusside(SNP)and N-nitro-l-arginine-mythel-ester (L-NAME) on apoptosis of spermatogenic cells in rats. Methods Fourty adult male Sprague-Dawley rats (60-70days) were divided into four groups.Each group was injected intraperitoneally with one of the following agents, once a day, for 12 days: 1. SNP; 2.L-NAME;3.SNL+L-NAME;4.Normal saline NS group.Two hours after the last time injection the rats were sacrificed.TUNEL staining and flow cytometry analysis were used to detect the apoptosis of spermatogenic cells. Results Sub-monoploid and apoptosis index (AI) in SNP group was significantly higher than that of NS group and sub-monoploid and apoptosis index (AI) in L-NAME group were significantly lower than that of NS group by FCM and TUNEL (P0.5) was found.Conclusion SNP can accelerate the apoptosis of spermatogenic cells and L-NAME can inhibite the apoptosis of spermatogenic cells,The effect of SNP and L-NAME on apoptosis of spermatogenic cells probably occurs through the action of nitric oxide.