Objective To investigate the role of P21 protein and survivin on the cell apoptosis of non-small cell lung cancer (NSCLC) induced by mevastatin. Methods The inhibitory effect of mevastatin on A549 and NCI-H520 cell line was evaluated by MTT assay. The cell cycle distribution and apoptosis induction were determined with flow cytometer and transmission electron microscope. The expression of p21 protein was assessed with flow cytometer. The mRNA expression of p21 and survivin was assessed with RT-PCR. Results Flow cytometry showed that mevastatin induced G_0/G_1 cell arrest in NSCLC cell lines. The results indicated that mevastatin caused apoptosis in concentration-dependent manner. Mevastatin produced no change in expression of P21 mRNA and total P21 protein. Concomitantly, P21 protein localized on cellular membrane was decreased. It was also found that mevastatin suppressed the expression of survivin mRNA in NSCLC cell lines. All these effects were reversed by mevalonate. Conclusions Mevastatin inhibited the proliferation of NSCLC cell lines. Mevastatin exerts growth inhibitory effect and induces apoptosis effect by inhibiting mevalonate synthesis. Its mechanisms might involve blockade of the isoprenylation of p21 protein and down-regulation of the expression of survivin mRNA.

Objective To investigate the role of P21 protein and XIAP on apoptosis-inducing effect by mevastatin against A549 cell line and its mechanisms. Methods The cells proliferation was detected by MTT assay. The cell cycle distribution and apoptosis induction were evaluated with flow cytometer and electron microscopy. The mRNA expression of P21 and XIAP was measured with reverse transcription-polymerase chain reaction.The protein expression of P21 and XIAP was tested with flow cytometer and Western Bolt respectively. Results Mevastatin inhibited A549 cell survival in a time-dependent and concentration-depended manner. Flow cytometry showed that mevastatin induced G0/G1 phase arrest and caused apoptosis. Mevastatin did not affect P21 expression at both mRNA and total protein level.Concomitantly,P21 protein localized on cellular membrane decreased.Both mRNA and protein expression in XIAP were down regulated by mevastatin. All these effects were reversed by mevalonate.Conclusion Mevastatin can inhibite proliferation,induce apoptosis and interfere with cell progression of A549 through mevalonate pathway. Its mechanisms are explained by decreasing the expression of XIAP mRNA and protern.Targeting HMG-CoA reductase,Mevastatin blocks the isoprenylation of P21 protein which affects its anchorage on the cellular membrane.

Objective To understand the current situation of medical postgraduates'!honesty edu-cation, find the weak point and explore new ways of the honesty education. Methods The honesty educa-tion survey questionnaire was designed and conducted to the postgraduates of Grade 2014 in Peking University Health Science Center. The research contents included the basic information of postgraduates, honesty cognition, clean and self-discipline, honesty education, the recommendation of honesty education and the application of honesty education carrier. 1 038 questionnaires of a total of 1 142 questionnaires were recovered and the recovery rate was 90.9%. EPIData3.0 software was used for double entry and database establishment, and SPSS 19.0 software for statistical treatment. Results 82.3% (854) of the postgraduates recognized the work of clean government, but only 62.1% (644) of the postgraduates concerned about it. 78.3% (813) of the postgraduates expressed hatred for the dishonest behavior and hoped to eliminate it. 77.6%(806) postgraduates often paid attention to honest education content which was pushed by the new media. 16.5% (171) postgraduates thought that the new media was very important to the honest cognition. Conclusions We should pay more attention to medical postgraduates'!honesty education, find its weak points according to the investigation and make full use of the network and new media. And at the same time we should combine the actual environment to establish a hierarchy of honesty education system, and carry out honesty education through total environment which contains the school administrators, instructors/head teachers and tutors.

Objective To observe the effect of crawling exercise and bilateral electrical stimulation on the upper limb motor after stroke. Methods 60 stroke patients were divided into experimental group (n=30) and control group (n=30). Both groups received routine therapy. The control group received unilateral electrical stimulation on the upper limbs, and the experimental group received electrical stimulation on bilateral upper limbs and crawling exercise. All the patients were assessed with the modified Ashworth scale (MAS), the Fugl-Meyer assessment (FMA) and the modified Barthel index (MBI) before and after treatment. Results After 8 weeks of treatment, the scores of MAS, FMA and MBI improved in both groups (P<0.05), and improved more in the experimental group than in the control group (P<0.05). Conclusion The crawling exercise and bilateral electrical stimulation can improve the motor of upper limbs and activities of daily living more effectively than the unilateral electrical stimulation after stroke.

BACKGROUND:At present, the most common materials for clinical hemostasis are absorbable tampons and Vaseline gauze. OBJECTIVE:To compare the packing effects of absorbable tampons and Vaseline gauze in functional endoscopic sinus surgery. METHODS:Totaly 100 sinusitis patients, 57 males and 43 females, aged 16-61 years, with a history of 5 months to 23 years, were randomized into two groups: patients in the observation group were treated with absorbable tampons and patients in the control group treated with Vaseline gauze during functional endoscopic sinus surgery. Patient's discomfort, bleeding when the filer was extracted at 48 hours, and nasal bleeding within postoperative 24 hours were compared between the two groups. RESULTS AND CONCLUSION: No adverse reaction related to absorbable tampons occurred in the observation group, indicating that the absorbable tampon has better histocompatibility. Patient's discomfort, bleeding when the filer was extracted at 48 hours after operation, and nasal bleeding within postoperative 24 hours were significantly improved in the observation group compared with the control group (P < 0.05). These findings suggest that the absorbable tampons has a better hemostatic function, which can effectively reduce postoperative nasal bleeding and relieve postoperative discomfort in patients with sinusitis.

Objective To investigate the effects of sodium ozagrel and berberine hydrochloride in the treatment of acute cerebral infarction and its effect on glucose and lipid metabolism.Methods According to the digital table,158 patients with acute cerebral infarction were randomly divided into the two groups:the control group was treated with the sodium ozagrel group and the observation group was treated combined with sodium ozagrel and berberine hydrochloride.After 2 weeks,Chinese stroke clinical neural function defect score scale and the National Institutes of Health Stroke Scale (NIHSS) was used to evaluate neurological function.The therapeutic effectiveness was assessed by ADL after treatment 1,3,6 months.The changes of glucose and lipid levels were detected by turbidimetric method.Results The total effective ratein the observation group was obviously higher than that of the control group (87.01％ vs 69.14％) (x2 =7.316,P ＜0.01).Of the above two ways of nerve function score indicateed that the observation group was significantly better than that of the control group (t =3.207,1.274,all P ＜ 0.01).ADL score of all patients was significantly better than that of before treatment with drug in the two groups after 1 month,3 months and 6 months (t =7.266 and t =8.850,t =1.429 and t =2.816,t =9.218 and t =6.739,P ＜0.01).Compared with the control group,ADL score of the observation group was significantly improved after 3 months and 6 months (t =1.163,0.932,all P ＜0.01).The significant difference was found between the control group and the observation group after 3 months and 6 months (t =7.861,5.005,all P ＞ 0.05).After the treatment,the fasting blood glucose level was reduced significantly in the observation group (t =0.859,P ＜ 0.01),total cholesteroland high-density lipoprotein,triglyceride,low density lipoprotein level were significantly decreased (t =0.257,0.114,0.378,all P ＜ 0.01),but there was no significant difference in HDL-C level (t =1.960,P ＞ 0.05).Conclusion The therapeutic effect of berberine hydrochloride combined with sodium ozagrel is better in the treatment of acute cerebral infarction,and the level of glucose and lipid metabolism was significantly decreased.

Objective To investigate different media-use habits and different media information comprehension of the audiences.Methods 127 inpatients and 33 outpatients were sampled for questionnaire survey,learning such information as the basics of the audience,medical experience and current media use,participation intension analysis.Results Internet,television,newspapers and other mass media constitute main channels of the audiences to learn health knowledge; audiences depend on the media to know the departments and specialists of the hospital Conclusion in the meantime of professional disciplines development and innovation,the hospital should not ignore its branding and innovation; an important management tool in its branding strategy is to integrate hospital and media resources,build positive image of the hospital,in order to improve hospital brand awareness,credibility and reputation

Objective To investigate the levels of transformation growth factor-β1 (TGF-β1),vascular endothelial growth factor (VEGF) and insulin growth factor-Ⅰ (IGF-Ⅰ) role in the pathogenesis of serum of patients with combined pulmonary fibrosis and emphysema (CPFE).Methods Recruited 20 patients with CPFE,40 cases with idiopathic pulmonary interstitial fibrosis (IPF) and 40 cases with emphysema who were admitted to our hospital during July 2011 to February 2012.Serum levels of TGF-β1,VEGF and IGF-Ⅰ were detected by ABC-ELISA.Results Serum levels of TGF-β1 and IGF-Ⅰ were significantly higher in patients with CPFE and IPF than these in patients with emphysema (TGF-β1:(160.73 ± 40.62) ng/L vs.(167.35 ± 42.82) ng/L vs.(128.75 ±35.77) ng/L; IGF-Ⅰ:(179.65 ±60.73) ng/L vs.(192.32 ±58.75) ng/L vs.(148.73 ±49.67) ng/L,P ＜ 0.05 or P ＜ 0.01).The IPF group had significantly higher serum level of VEGF than the emphysema group ((506.12 ±82.37) ng/L vs.(437.31 ±62.58) ng/L,P ＜0.01).Serum levels of TGF-β1 and IGF-Ⅰ in CPFE and emphysema groups were positively correlated (r =0.885,0.918 respectively,P ＜ 0.01).Smokers in the CPFE group had significandy lower level of serum VEGF than those who did no smoke ((406.19 ± 66.94) ng/L vs.(482.88 ± 79.91) ng/L,t =-2.287,P =0.035).Conclusion Serum level of VEGF increased significantly in the IPF group,suggesting the participation of VEGF in pulmonary interstitial fibrosis.IGF-Ⅰ involved in the pathogenesis of pulmonary interstitial fibrosis.TGF-β1 and IGF-Ⅰ have a positive linear regressive relationship,which indicates that they may work synergistically in the process of the fibrosis.

Objective To explore the Activation function of the bifidobacterium adolescence cell wall(BCW)on macrophage in abdominal cavity of mice and tumor-inhibition of BCW on human transitional cell carcinoma of bladder(BTCC)in vitro.Methods ①Twenty KM mice were randomly divided into two groups:control group(n=10)and BCW injection group(n=10).BCW was distilled by ultrasonic cell disintegrator.Then the BCW was injected into abdominal cavity of the BCW injection group mice,physiological saline was injected into the control group mice,then all the mice were killled,macrophage in abdominal cavity was collected under the condition of asepsis,the level of IL-2 and IFN-? which produced by macrophage was determined by ELISA reagent box;②The role of tumor-inhibition of BCW on human transitional cell carcinoma of bladder(BTCC,T24)in vitro was determined by MTT method:The cell of T24 in the stage of logarithmic growth was digested,inoculated in cell raise board,cultivated after 24 hours,different concentration of BCW were added into each hole,cultivated continually,replaced nutrient fluid on 1th day,3th day,5th day,MTT was added into each hole too,cultivated continually,DMSO was added into each hole,vibrated,shaked up,determined the A value at 490nm by ELIASA,calculated the tumor-inhibition rate;Observed the growth state under microscope:The cell of T24 in the stage of logarithmic growth was inoculated in cell raise board that comprise microscope slides,cultivated after 24 hours,BCW were added into each hole,cultivated continually,took out two slides after 24 hours,72 hours,120 hours,stained,observed the change of the shape of T24 under microscope,changed the culture liquid only on control group.each time.Results The level of IL-2 and IFN-? of BCW injection group was higher than of control group(P

Objective To investigate the effects of IL-6/STAT3 activation on the proliferation of biliary epithelial cell following liver transplantation in rat.Methods Based on the classic "two-cuff" technique for orthotopic liver transplantation in rat,end-to-end anastomosis between the common hepatic arteries of donor and recipient by the modified "Gao stent" was performed to reconstruct hepatic artery blood flow.Wistar rats were then randomly divided into CP1h group and CP12h group(grafts were preserved in UW solution at 4℃ for 1h and 12h respectively),RPM group(CP12h group treated by rapamycin,RPM)and C group(control).At 1,3,7,14d after operation,liver IL-6 mRNA expression was analyzed by real-time RT-PCR.STAT3 activation was determined using laser confocal scan microscopy(LSM).BEC proliferation was detected by immunohistochemistry.Results In the CP1h group,expression of IL-6 mRNA was upregulated one day after operation(0.41?0.03),and then fell to the C group level subsequently(0.28?0.03,0.23?0.03 and 0.27?0.05 at 3,7 and 14d after operation).STAT3 activation in the BEC(94?8,61?6,39?4 and 34?3)and proliferation of the BEC following liver transplantation(2.1%?0.3%,5.9%?0.5%,2.6%?0.5% and 2.3%?0.5%)showed a similar trend.Compared with the CP1h and C group,the CP12h group demonstrated a significant and persistent up-regulation of IL-6 mRNA(0.60?0.03,0.73?0.02,0.38?0.02 and 0.30?0.04)and STAT3 activation(167?17,247?13,110?9 and 74?8).BEC proliferation in CP12h group were 7.0%?0.5%,27.8%?1.8%,23.1%?1.6% and 17.8%?1.2%,there was a direct correlation between IL-6 mRNA expression and STAT3 activation(r=0.95,P