0.05).Conclusion The reduced expression of FHIT may be associated with endometrium canceration and may be not associated with surgical pathologic staging,lymph node metastatic and depth of myometrial invasion in endometrial carcinoma.The reduced expression of FHIT indicates the pathogenesis of endometrial carcinoma.

Objective To observe the effect and the influence on carotid atherosclerotic plaques,C-reactive protein,lipids,coagulation of atorvastatin and xuesaitong injection in the treatment of elderly patients with acute cere-bral infarction.Methods 100 elderly patients with acute cerebral infarction met inclusion criteria were randomly divided into 50 cases of the observation group and 50 cases of the control group,the control group were given conven-tional therapy,the observation group were given atorvastatin and xuesaitong injection on the basis of the control group, atorvastatin calcium 20mg/d,qd,Xuesetong 400mg/times,qd,both groups had been treated for 3 weeks for a course of treatment,the serum C-reactive protein,serum lipids and coagulation parameters were detected before and after treat-ment,the IMT and carotid plaque area were determined by color Doppler ultrasonography,NIHSS score were calculat-ed.Results The PT,APTT,CRP,IMT,carotid plaque area,NIHSS score of the observation group were (18.07 ± 2.24)s,(36.59 ±3.52)s,(11.2 ±3.6)mg/L,(0.57 ±0.16)mm,(1.36 ±0.32)cm2,(9.31 ±2.06)point,the observation group were (15.24 ±1.88) s,(31.84 ±2.55) s,(26.4 ±6.7) mg/L,(0.68 ±0.13) mm,(1.55 ± 0.37)cm2,(15.86 ±2.25)point,both groups were significantly improved after treatment than before treatment (all P<0.05),The total efficiency of observation group and control group were 96%and 72%,the observation group was significantly higher than those of the control group, the difference was statistically significant (χ2 =10.867, P <0.01).Conclusion The method containing atorvastatin and Xuesaitong injection can effectively regulate blood lipid levels and blood coagulation,inhibit vascular inflammation,Stabilizing or reversing carotid artery plaque,reduce carot-id intima-media thickness,can inhibit thrombosis and progress,improve cerebral hypoxia and ischemia parts neurologi-cal defects,improve clinical outcomes,significantly improved clinical outcomes.

Objective To establish quality standard for prepared slices of salt-processed psoralea corylifolia.Methods Psoralen and isopsoraten in the crude psoralea corylifolia and its salt-processed products were determined by traditional identification,thin-layer identification and HPLC method.Water,ash,extract,pesticide residue and heavy metals were also detected.Results The average content of total content determination for psoralen and isopsoraten in more than 10 patches of crude and processed samples was not below 0.5% and 0.6% respectively.Conclusion HPLC method is simple and the result is reliable with good repeatability and can be used for the determination of the components in psoralea corylifolia.The quality control standards of the psoralea corylifolia is normative,systematic and the result is accurate.

OBJECTIVE:To evaluate the utilization and tendency of calcium products and calcium absorption enhancers in our hospital.METHODS:The calcium products and calcium absorption enhancers used in our hospital from 2005 to 2007 were analyzed statistically in respect of drug variety,consumption quantity,consumption sum and DDDs.RESULTS:Compound Calcium Amino Acid Chelate Capsules and Calcium Carbonate Chewable Tablets took the lead in clinical application.Leading the list of DDDs were Compound Calcium Amino Acid Chelate Capsules(Osteoform Capsules),Calcium Carbonate Chewable Tablets and Oyster Shell Tablets Calcium,however,the old generation calcium products had gradually become out of date.CONCLUSION:The use of calcium products and calcium absorption enhancers in our hospital was rational on the whole,but there are some problems which call for attention.

Objective:To establish a method for simultaneously determinating the content of 10 constituents including chlorogenic acid, puerarin, forsythoside A, liquiritin, matrine, saikosaponin A, saikosaponin D, forsythin, ammonium glycyrrhizate, indirubin in Xiao’er-Jinqiao granules by UPLC. Methods:ACQUITY UPLCTMC18 chromatographic column (100 mm × 2.1 mm, 1.7 μm) was used. The mobile phase was acetonitrile-0.05% phosphoric acid solution with gradient elution at the flow rate of 0.2 ml/min. The detection wavelength was 250 nm. The column temperature was 30 ℃. The injection volume was 2 μl.Results:The linear ranges of chlorogenic acid, puerarin, forsythoside A, liquiritin, matrine, saikosaponin A, saikosaponin D, forsythin, ammonium glycyrrhizate, indirubin were 0.789 5-15.793 4 μg ( r=0.999 6), 0.571 8-11.445 6 μg ( r=0.999 4), 0.110 7-2.214 7 μg ( r=0.999 3), 0.047 5-0.950 5 μg ( r=0.999 2), 0.269 8-5.395 8 μg ( r=0.999 7), 0.085 1-1.703 9 μg ( r= 0.999 5), 0.105 7-2.113 0 μg ( r=0.999 4), 0.065 6-1.312 1 μg ( r=0.999 3), 0.080 5-1.611 2 μg ( r=0.999 5), 0.057 7-1.153 3 μg ( r=0.999 4). The limits of quantitation were 8.322, 9.023, 9.857, 4.583, 6.872, 7.844, 8.643, 5.895, 7.568, 5.251 μg/ml. The average recovery rates were 99.11%, 98.87%, 97.83%, 98.10%, 98.17%, 98.30%, 98.10%, 98.14%, 97.99% and 98.02%, and RSDs were all below 2.0%, respectively. RSDs of precision, reproducibility and stability tests were lower than 2.0%. Conclusions:The established method of multi-component content determination is fast, accurate and reproducible. It can be used for the quality control of Xiao’er-Jinqiao granules.

Objective To analyze the incidence and risk factors of microalbuminuria in newly diagnosed and hospitalized type 2 diabetes mellitus patients,and to provide the theoretical basis for therapy of diabetes and diabetic chronic complications.Methods The blood glucose,lipid profile,24h-urinaryalbumin,fasting and postprandial serum insulin and peptid C were measured in 286 newly diagnosed and hospitalized type 2 diabetic patients from Mar.2006 to Sep.2009.Microalbuminuria was defined as the 24h-urinary albumin between 30 to 300 mg twice.Insulin resistance index (HOMA-IR) was recorded and all the patients received fundus examination by ophthalmologists after expansion of the pupil to ensure whether there was retinopathy or not.Results (1)The incidence rate of microalbuminuria in this study was 19.58％ (56/286).(2)Normal albuminuria group and MA group combined DR were 19.57％ (45/230) and 33.93 ％ ( 19/56 ) respectively,and the difference was statistically significant( x2 =5.349,P =0.021 ).Compared with control,the FPG,HbA1c and HOMA-IR were significantly higher in microalbuminuria group ( FPG:[ 11.08 ± 1.76 ] mmol/L vs [9.24 ±1.65]mmol/L,t =-6.148,P ＜0.001;HbA1c:[11.54 ± 1.59]％ vs [9.39 ± 1.64]％,t =-7.533,P ＜0.001 ;HOMA-IR:[3.73 ±0.42] vs [3.50 ±0.30],t =-3.774,P ＜0.001 ).(3) Pearson correlation analysis revealed microalbuminuria was positively correlated with FPG( r =0.460),HbA1 c ( r =0.499),HOMA-IR( r =0.308)and combined DR(r =0.405)(P ＜0.01).(4)Stepwise multiple regression analysis revealed that FPG (ββ =- 0.804,P ＜ 0.01 ),HbA1 c (β =- 0.455,P ＜ 0.01 ) and combined DR (β =1.527,P ＜ 0.05 ) were independently risk factors of microalbuminuria.Conclusion The development of microalbuminuria in newly diagnosed and hospitalized type 2 diabetic patients is associated with FPG,HbA1c and HOMA-IR,and will be easier to merge diabetic retinopathy.

Objective: To evaluate the analgesic effect of pregabalin combined with intrathecal sufentanil infusion for the treatment of breakthrough pain in patients with bone metastases. Methods: A total of 60 breakthrough pain patients with bone metastases were randomly divided to 3 groups: group A (pregabalin combined with intrathecal sufentanil infusion group,n=20), group B (placebo combined with intrathecal sufentanil infusion group,n=20) and group C (oral morphine sulfate controlled-release tablet group,n=20). The differences in visual analogue scale (VAS)between background pain and breakthrough pain, the seizure frequency of breakthrough pain, general satisfaction and side effects of the 3 groups were observed. Results: hTe seizure frequency and VAS of breakthrough pain in group A decreased signiifcantly after the treatment (P<0.05) and the general satisfaction was the best among the the 3 groups (P<0.05), with less nausea and vomiting, constipation, drowsiness and fewer other side effects. Conclusion: Pregabalin combined with intrathecal sufentanil infusion can effectively relieve breakthrough pain in patients with bone metastases.

Objective To study the prevalence of atopic dermatitis (AD) in children in major cities of China. Method The children aged 1-7 were surveyed with questionnaire in ten cities of our country. Results In a total population of 49 241, there were 1 371 AD patients (768 males and 603 females) discovered. The total prevalence was 2.78%. The prevalence of males and females was 3.03% and 2.53%, respectively, The total standardized prevalence (S.P.) was 3.07%. The male and female S.P. was 3.86% and 2.20%, respectively, the difference being statistically significant (P

Objective:To observe the expressions of Th22 cells and IL-22 in peripheral blood of patients with Lupus nephritis (LN) and explore its significance.Methods: Patients of systemic lupus erythematosus (SLE) with no renal involvement (n=38),lupus nephritis (n=32) and healthy controls (n=10) were studied.Flow cytometry was utilized to quantify the percentage of Th22 cells in peripheral blood.Enzyme-linked immunosorbent assay was used to detect the levels of serum IL-22.The Th22 cells and The serum level of IL-22 among three groups were compared,and the correlation between Th22,IL-22 and SLE disease activity index score (SLEDAI) was analyzed.Results: Th22 cells in SLE group and LN group were significantly higher than those in healthy control group (P<0.01).Serum IL-22 level was also significantly higher than that of healthy control group (P<0.01);Th22 and IL-22 LN group were slightly higher than that in SLE group,but the difference was not significant (P>0.05).The proportion of Th22 cells and the level of IL-22 in LN group were positively correlated with the disease activity score SLEDAI.Conclusion: Th22 cells and IL-22 may play an important role in the pathogenesis of SLE and LN.

Objective To study the expressions of matrix metalloproteinases-2 (MMP-2) and its tissue inhibitor-2 (TIMP-2) and microvessel density (MVD) in human endometrial carcinoma tissues and their relations with the invasion and metastasis of endometrial carcinoma. Methods Immunohistochemistry was used to measure the MMP-2, TIMP-2, and MVD protein levels in 33 patients with endometrial carcinoma and 8 normal endometrial samples. Results The MMP-2, TIMP-2 and MVD protein expressed in both normal endometrial samples and endometrial carcinoma. The positive expression proportions of MMP-2, TIMP-2, and MVD protein in endometrial carcinoma tissues were higher than those in normal endometrial samples (P