Objective To investigate the efficacy of auricular plaster therapy for post-laparoscopic cholecystectomy nausea and vomiting. Methods Two hundred and forty laparoscopic cholecystectomy patients were randomly allocated to treatment and control groups, 120 cases each. Both groups received routine nursing care. In addition, the treatment group received auricular plaster therapy. The incidence of nausea and vomiting was observed in the two groups.Results The incidence of nausea and vomiting was 46.7% in the treatment group and 60.8% in the control group; there was a statistically significant difference between the two groups (P<0.05).Conclusion Auricular plaster therapy can relieve postoperative nausea and vomiting in laparoscopic cholecystectomy patients.

Objective: To study the rehabilitation efficiency of adjuvant derma transplantation in treatment of front palate fistula. To find a better way to solve the problem of correcting the front fistula in short of the nearby tissue. Methods: Established the front fistula model in the oral and nasal cavity in the front of the palate with surgery. 30 Japanese big ear rabbits were divided into 2 groups. One month after operation, 15 rabbits were operated with the nearby tissue into the inner of nasal cavity and by transplanting their back skin while the others were treated only with the nearby tissues. The rehabilitation process and the histological changes of the palate were observed. Results: All dermal flaps survived after transplantation. This surgical method got a satisfying effect(P<0.05). The rate of success for correcting with single nearby tissues was lower. Conclusion: Adjuvant derma transplantation for rehabilitation of the front palate fistula is effective and reliable in the rabbits. The combination of the nearby tissue and homeochronous derma transplantation might be a good mode for correcting the front fistula in short of the nearby tissues.

Objective To investigate the mechanism of xCT on tumor metastasis in breast cancer cell MDA-MB-231. Methods Wound scratch assay and Transwell assay were performed to evaluate the effect of disruption and knockdown of xCT on cell migration and cell invasion in breast cancer cell MDA-MB-231 .Western Blot and RT-PCR were used to detect the expression levels of autophagy and EMT related markers in breast cancer cell MDA-MB-231 after treatment with sulfasalazine (SASP), an inhibitor of xCT activity and SLC7A11-RNAi.Results Both the scratch assay and the transwell migration assay showed that inhibition of xCT reduced the motility of MDA-MB-231 .The expression level of autophagy related protein LC3-Ⅱ/LC3-Ⅰwas elevated, the protein level of transcription factor Snail was down-regulated, while the mRNA level of Snail did not change in xCT inhibited MDA-MB-231 cells compared with MDA-MB-231 cells.Epithelial marker E-cadherin was up-regulated but mesenchymal marker Vimentin was down-regulated when xCT was deficient.Con-clusion Our current studies show that xCT is an endogenous regulator of tumor growth and metastasis in MDA -MB-231 and the expression level of xCT determines the phenotypes of MDA-MB-231 cells in invasion and migration in vitro.Inhibition of xCT can activate autophagy , induce the degradation of Snail ,and attenuate the EMT process in highly metastatic MDA-MB-231 cells.

BACKGROUND:Acute myocardial infarction with acute onset is dangerous, but the aided diagnosis for hyperacute disease mainly depends on electrocardiogram. The advantages of tissue Doppler strain imaging were utilized to help early diagnosis of acute myocardial infarction.
OBJECTIVE:To observe left ventricular transmural peak radial strain and strain time-to-peak of subendocardiac muscle, midmyocardium and subepicardiac muscle using tissue Doppler strain imaging in dogs before and after acute myocardial infarction, and to assess its mechanical characteristics.
METHODS:A total of 16 Beagle dog models of acute myocardial ischemia were established by ligating left anterior descending coronary artery. The two-dimensional apical short-axis views of the left ventricle in five complete cardiac cycles were acquired and stored in TDI-Q workstation before and after acute myocardial ischemia. Transmural peak radial strain and strain time-to-peak of segment, subendocardiac muscle, midmyocardium and subepicardiac muscle at infarct region and baseline were observed.
RESULTS AND CONCLUSION:Peak radial strains at infarct and subendocardiac muscle, midmyocardium and subepicardiac muscle were decreased compared with the baseline (P<0.05). Peak strain gradient disappeared in each layer of infarct myocardium. Strain time-to-peak of the whole segment and infarct myocardium at different layers was significantly postponed (P<0.05). There was a positive correlation of peak radial strain between subendocardium and segment as wel as between medium and segment at baseline (r=0.617, P<0.01;r=0.556, P<0.01). This relationship disappeared at infarct region (r=0.338, P>0.05;r=0.218, P>0.05). Results indicated that after acute myocardial infarction, peak strain gradient disappeared at different layers at infarct region. Acute myocardial ischemia induces peak radial strain decrease at subendocardium, medium, subepicardium and strain time-to-peak at infarct region was significantly postponed, which reflected abnormal cardiac structure and dysfunction, resulted in uncoordinated cardiac motion and asynchronous heart movement. This may be an important mechanical mechanism triggering heart failure.

Red blood cell distribution width is an index reflecting the size heterogeneity of circulating red blood cells, which is usually used for diagnosis and treatment of blood system diseases.In recent years, more and more evidences show that the increase of red blood cell distribution width level is closely related to the occurrence, development and prognosis of cardiovascular diseases.We focused on the relationship between red blood cell distribution width and atrial fibrillation (AF) and its possible mechanism, in order to provide some reference value for the clinical treatment of AF.

Objective To investigate the effect of tongue tie corrective surgery on the development of language in children with short lingual frenulum.Methods 94 children with short lingual frenulum were selected,and under the permission of the patients'family they were randomly divided into two groups.The treatment group received ankylotomy and language training.The control group was only given language training.The clinical efficacy,improvement time of pronunciation and adverse reactions were compared between the two groups.Results The total effective rate of the treatment group was 91.49％,which was significantly higher than 72.34％ of the control group (x2 =5.816,P ＜0.05).The improvement time of pronunciation in the treatment group was significantly shorter than the control group(t =21.250,P ＜ 0.01).There were no serious adverse reactions observed in two groups.Conclusion Tongue tie correction surgery can significantly affect the language development in children with short lingual frenulum,the improvement time of pronunciation is short,which is worthy of clinical application.

Objective To investigate and compare the effect of orally or intranasally administered borneol on ketamine‐mid‐azolam anesthesia anesthetized rats′regained consciousness and cognitive functions .Methods The rats were trained to escape laten‐cy and divided into 6 groups:control group ,model group ,low dose oral borneol group ,high dose group oral borneol group ,low dose intranasal borneol group and high dose intranasal borneol group ,16 rats in each group .All groups were given general anesthesia ,be‐side the control group .The reflex recovery time ,blood gas analysis ,escape latency and space exploration capability were recorded and compared within groups .Results Compared with the control group ,PaCO2 ,P(A‐a)DO2 and latent escape time increased (P<0 .05) ,pH ,PaO2 and times of platform across decreased in the model group (P<0 .05) .Compared with the model group ,the righ‐ting reflex test time ,times of platform across and latent escape time decreased (P<0 .05) ,blood gas analysis improved (P<0 .05) in intranasal borneol groups and oral borneol groups .The righting reflex test time ,blood gas analysis and times of platform across had no significantly differences within borneol oral groups(P> 0 .05) .Compared with low dose oral borneol group ,latent escape time decreased in high dose oral borneol group (P<0 .05) .Compared with borneol oral groups ,the righting reflex test time ,times of platform across and latent escape time decreased (P< 0 .05) ,blood gas analysis improved (P< 0 .05) in intranasal borneol groups .Compared with low dose intranasal borneol group ,the righting reflex test time and latent escape time decreased (P<0 .05) , blood gas analysis improved (P<0 .05) in high dose intranasal borneol group .Conclusion Intranasal borneol could reduce righting reflex test time and improve cognitive function by improving the blood gas analysis .

OBJECTIVE:To analyze the current situation and developing trend of drugs used in hospitals of Chengdu.METHODS:The data of drugs purchased by 16 hospitals in Chengdu in the years 1997～2000 was collected and analysed.RESULTS:The drug expenses increased faster in years 1998～1999 and the drug expense in 2000 was 3 times as much as that in 1997.The proportion of sum of money spent on anti-infectives was decreasing year by year,which equaled that in developed cities of east China.CONCLUSION:The sum of money of drugs consumed will increase year by year,however,the growth rate may decrease.The proportion of consumption of imported drugs will continuously decrease.

Objective To observe the effect of electromyographic biofeedback combined with earlier rehabilitation on serum matrix metalloproteinase-9 (MMP-9) and its clinical efficacy for patients with cerebral infarction. Methods 90 patients with acute cerebral infarction were randomly divided into control group (n=30), rehabilitation group (n=30) and combination group (n=30). All the groups received basic medication, and the rehabilitation group combined with earlier rehabilitation, the combination group with electromyographic biofeedback and earlier rehabilitation. All patients were evaluated with Clinical Neurologic Functional Default Scoring (CNFDS) and the modified Barthel Index (MBI) before and 2 weeks after treatment. Their serum levels of MMP-9 were detected as well. Results The scores of CNFDS and MBI improved after treatment in all the groups (P<0.001), especially in the combination group (P<0.01). The levels of MMP-9 decreased more in combination group than in the rehabilitation group and the control group (P<0.001). Conclusion Electromyographic biofeedback combined with earlier rehabilitation can facilitate the recovery neural function, which may associated with the decrease of MMP-9.

Objective To explore the influence of hematopoietic growth factors on the TBI patients in the conditioning regimen for stem cell transplantation,and to evaluate the effect of cytokines on treatment of acute radiation disease.Methods The usage of hematopoietic growth factors,implantations and the side-effects of the patients and donors of TBI in the conditioning regimen for stem cell transplantation were retrospectively analysed from 1990 to 2012,and the effect and side-effects of cytokines on the hematopoietic function recovery and stem cell mobilization were observed.Results All patients recovered from their hematopoietic function except one died due to the side-effecs.The median time of white blood cell recover was 10 d in auto-SCT and 12 d in allo-SCT in the G-CSF group.The median day of platelet recovery was 11.67 ± 1.53 in rhIL-11 arm and 13.70 ±6.27 in no rhIL-11 arm in the auto-SCT group.The incidence rates of dental ulcer and diarrhea in the TBI patients were 48％ and 44％,respectively.The occurrence of side-effect was rare in the period of cytokines treatment,but was over 50％ during stem cell mobilization.Conclusions Cytokines play very important roles in the hematopoietic function recovery and stem cell mobilization in the TBI patients.