Objective:To construct an expression plasmid of siRNA against gene hIAP-2 and to assess its effect on breast cancer cell line MCF-7.Methods: A hIAP-2 siRNA plasmid was constructed with mU6pro vector containing U6 promotor and its RNAi effect on MCF-7 cells was detected by RT-PCR and Western blot.The effects of the plasmid on MCF-7 proliferation were analyzed by MTT assay,on the cell cycle by flow cytometry,and on the cell morphology by Hoechst staining.The activity of caspase-3 was determined with its substrate Ac-DEVD-pNA and the expression of some related proteins was detected by Western blot.Results: The hIAP-2 siRNA plasmid knocked down hIAP-2 expression in MCF-7 cells specifically and obviously,inhibited tumor cell proliferation and arrested the MCF-7 cells at G_(1) phase.After hIAP-2 gene was silenced,MCF-7 cells became multinuclear and had macronucleus;procaspase-3 was activated and its expression was increased.The protein levels of I?B?(p37) and p21~(waf1) increased,of NF-?B(p65) decreased and Cyt C remained unchanged.Conclusion: hIAP-2 in MCF-7 cells plays a crucial role in cell proliferation and cell cycle progression.Mitotic cell death might be the main way of the cell death;G_(1) arrest after DNA damage might be associated with the p21~(waf1) increase.

Objective To investigate the effects of different times of needle retention on cognitive function in patients with vascular cognitive impairment no dementia (VCIND). Methods One hundred and four VCIND patients were randomly allocated to group A of 35 cases, group B of 34 cases and group C of 35 cases. All the three groups received acupuncture plus medication. Of them, group A was given scalp acupuncture with 30 min retention of needles;group B, with 1 hr retention of needles;group C, with 10 hrs retention of needles. The Montreal Cognitive Assessment (MoCA) score and the ADL score were recorded in the three groups of patients before and after treatment. Results There were statistically significant pre-/post-treatment differences in the MoCA score and the ADL score in every group (P<0.05). There were statistically significant post-treatment differences in the MoCA score and the ADL score between group A or B and group C (P<0.05). Conclusions Acupuncture is an effective way to treat VCIND. It can improve cognitive function in the patients. Scalp acupuncture with 10 hrs retention of needles has a more marked effect.

With the development of global warming,urbanization,ageing of the population,it is paid more attention to the human health impacts on the frequent occurrence of heat wave and urban heat island effect.The study methods and advances in impacts of heat wave on human health were reviewed in the present paper.

Objective To explore the clinical efficacy of cetuximab combined FOLFOX chemotherapy on colon and colorectal cancer.Methods Forty patients with colon and colorectal cancer were randomly divided into observation group and control group,and 20 cases for each group.Patients in control group were treated with oxaliplatin + leucovorin + 5-fluorouracil,and in the observation group were given cetuximab (the first does was 400 mg/m2 at the first week,then the second week and follow weeks was 250 mg/m2 1 time/week for 6 week) plus the treatment as control group.The clinical efficacy,adverse effect and 1-year survival in the two groups were recorded.Results The total effective rate and clinical benefit rate in observation group were 60％ (12/20)and 85％ (17/20) respectively,which significantly higher than the control group (30％ (6/20) and 65 ％ (13/ 20),x2 =18.18,10.67,P ＜ 0.05).Skin rash rate in observation group was 55％ (11/20),which was significantly higher than that of control group (15％ (3/20),x2 =35.16,P ＜0.05).Half-year survival rate and 1-year survival rate of patients in observation group were 75％ (15/20) and 45％ (9/20) respectively,significantly higher than the control group (50％ (10/20) and 25％ (5/20),x2 =13.33,8.791,P ＜ 0.05)~ Conclusion Treatment scheme of Cetuximab combined FOLFOX chemotherapy is proved to be an effect of treating advanced colon and colorectal and be with the low adverse effect.

ObjectiveTo investigate the correlation of TIMI risk score and plasma brain natriuretic peptide (BNP) in patients with Acute myocardial infarction. MethodsTIMI risk score were calculated in 63 patients with acute myocardial infarction;At the same time plasma brain natriuretic peptide were measured by enzyme-linked immune absorption assay(ELISA). Analyze the correlation of TIMI risk score and plasma brain natriuretic peptide. ResultsNine patients of 63 occurred cardiavascular events during hospitalization or follow-up( 14.3％ ). The incidence of MACE increased significantly with the rise of TIMI risk score and BNP level. The TIMI risk score was positively correlated with the plasma BNP level( r =0.73 ,P ＜ 0. 05 ). ConclusionThe higher TIMI risk score and BNP level, the worse the prognosis, it could be use to predict the prognosis.

BACKGROUND: Study on epidermal stem cells (ESCs) is satisfying. However, the amount and the distribution dynamic changing rule in each skin layer of ESCs during survival of skin graft need to be investigated.OBJECTIVE: To observe the changing rule of ESCs in the microenvironment during the survival of full-thickness skin graft.DESIGN: Prospective animal experiment based on controlled observation.SETTTNG: Department of Burn and Plastic Surgery, First Hospital Affiliated to Nanhua University.MATERIALS: Forty-two Wistar rats of clean grade and either gender, aged 2 to 3 months, weighing about 200 to 250 g,were provided by the Experimental Animal Center of South China University. After being raised for 1 week, the rats were divided into 7 groups according to observation time: post-operation 24 hours, 3 days, 5 days, 7 days, 14 days, 21 days and 30 days groups, with 6 rats in each group. Primary antibody: rabbit anti-rat integrinβ1 polyclonal antibody (Boster Biological Company, Wuhan); mouse anti-rat p63 monoclonal antibody (Santa cruz Company); Second antibody: goat anti-rabbit lgG (Zhongshan Biological Company, Beijing), goat anti-mouse lgG (Zhongshan Biological Company, Beijing);S-P immunohistochemical kit and DAB kit (Zhongshan Biological Company, Beijing).METHODS: This experiment was carried out in the Animal Laboratory of Nanhua University in May 2006. ①An area of (1.5×1.5) cm2 autologous full thickness skin graft model was made on the back of the all the rats. The color change of skin graft was observed at different time points. Meanwhile, whether hematocele and fluidify as well as binding to wound appeared under the skin graft were investigated. ②During the operation, a few skin graft was taken from each rat and immediately fixed with 100 g/L neutral formaldehyde solution and labeled, serving as blank control group. ③Skin graft packages of rats in the post-operation 24 hours, 3 days, 5 days, 7 days and 14 days were open at corresponding time points. Skin tissues were cut from the center of each wound and labeled; Skin graft packages of rats in post-operation 21 days and 30 days groups were open at postoperative 14 days. Rats in the two groups were raised till post-operation 21 days and 30 days, respectively, and skin tissue was cut from the center of skin graft. ④Pathological sections were made for haematoxylin and eosin (HE) staining and immunohistochemical staining.MAIN OUTCOME MEASURES: ①The amount of inflammatory cells, epithelization degree and the level of fibrocytes in the section. ② Integrin β1 and gene p 63 positive cell rate.RESULTS: Forty-two Wistar rats were involved in the final analysis. ①Gross observation of wound: At postoperative 1 to 2 days, the skin graft was pale and edematous; At postoperative 3 days, the color of skin graft did not turn better, but skin graft closely attached to the wound; At postoperative 7 days, the color of skin graft obviously turned better,epidermal color was close to normal, skin graft more closely attached to the wound, and some hair roots grew out of the wound; At postoperative 14 days, wound border and wound had healed completely; At postoperative 30 days, the color of skin graft became darker than normal, and the hair grew in the wound as in the normal skin. ②Observation results of HE stained pathological sections: Cellular edema and small quantity of inflammatory cell infiltration were found in the skin graft of the former 4 groups; Partial keratinocytes were necrotic and fell off at postoperative 5 days. ③Changing rule of distribution and amount of positive cells: Before operation, β1 integrin and gene p63 positive cells were mainly distributed in the epidermal basal membrane and eminence of hair follicle. Positive cells appeared in each epidermal layer from postoperative 24 hours and gradually increased. At postoperative 14 days and 21 days, many positive cells appeared in each epidermal layer. At postoperative 30 days, positive cells were distributed mainly in the basal layer of epidermis, and slightly in the other layers. Among the immunohistochemical sections, genic substance p63 positive cells were more thanβ1integrin positive cells at the same time point. p63 positive cells began to decrease from postoperative 24 hours,reached the lowest level at postoperative 3 days, then were gradually increased, reached or were close to the preoperative level at postoperative 7 days, reached the highest level at postoperative 21 days, subsequently were gradually decreased, and were still higher than preoperative level at postoperative 30 days.CONCLUSION: ①During the survival of full-thickness skin graft, ESCs are firstly decreased, then gradually increased, and even over the level in the normal skin. ②During the survival of full-thickness skin graft, firstly, ESCs arrange disorderly in the skin, and then they gradually gather in the epidermal basal membrane and eminence of hair follicle.

Objective To discuss the effect of modified venipuncture method on success rate of vcnipuncture, in order to alleviate the suffering of patients with emergency transfusion. Methods 380 cases of transfusion patients were randomly divided into the routine group and the modified group, rou-tine and modified venipuncture methods were applied to each group respectively. The success rate and pain response of each group were compared between the two groups by χ2 test. Results The success rate of venipuncture of the modified group was 93.50%, which was significantly higher than that of the routine group, and the rate of pain of the modified group was lighter than that of the routine group. Conclusions The modified venipuncture method can alleviate pain of patients, improve work efficien-cy and was worthy of clinical nursing application.

Objective To study and compare the metabolism of Chrysanthemum morifolium extracts(CME) in vitro intestinal flora from human,rat,Beagle dog,and rabbit.Methods Intestinal flora of human,rat,Beagle dog,and rabbit and CME were anaerobically cultured at 37 ℃ in vitro. After being extracted by acetic ether,the main metabolites of CME were separated and quantified by HPLC.Results In vitro,CME was easily metabolized by the intestinal flora and the main metabolites in incubation medium were determined in high concentration after 0.5 h.By LC-MS,luteolin and apigenin were identified,respectively;both metabolites were degraded with prolongation of incubation time and the concentrations of luteolin and apigenin were low after 24 h;moreover,CME was quickly metabolized by human,rat,Beagle dog intestinal flora,and gently by rabbit intesinal flora.Conclusion In vitro,CME is easily metabolized by intestinal flora human,rat,Beagle dog,and rabbit and the main metabolites are luteolin and apigenin.

Objective To analyze the clinical feature of X-linked chronic granulomatous disease (X-CGD) and gene mutation of CYBB. Method The clinical data of X-CGD in one child and the results of CYBB gene detection in his family were reviewed. Results This boy had onset in the neonatal period and presented with recurrent severe pulmonary infection as his main manifestation. Results of nitroblue tetrazolium test (NBT) in both non-stimulation group and LPS stimulation group in the child were 0, and neutrophil oxidation index (NOI) was 1.15. Gene analysis showed a deletion mutation in exon 6 of CYBB gene in the child (579-582delATTA), which resulted in frameshift mutation started from coding sequence of 189—isoleucine (I) and stop codon occured in advance in the 212th amino acid (I189fsX212). Both the child's mother and grandmother were carriers of the mutated gene. The same deletion mutation was not found in the CYBB gene in the amniocyte from the mother's next child. Conclusion One case of X-CGD patient with CYBB gene mutation and his families were diagnosed by gene detection. Prenatal diagnosis can avoid the birth of children with X-CGD.

AIM: To investigate the protective effects of quercetin on nephrotoxicity induced by cyclosporine A(CsA)in rats. METHODS: Male Sprague-Dawley (SD) rats were divided into four groups of seven rats each. Control group, quercetin (40 mg/kg) group, CsA (40mg/kg) group, CsA (40 mg/kg) plus quercetin (40 mg/kg) group. The all groups were administered once day respectively for 15 days. Twenty-four hour urines were collected at the 10th and 14th day respectively after administration to measure the content of urinary protein and urinary N-acetyl-?-D-glucosaminidase(NAG). The animals in all groups were sacrificed 4 h after the last administration. Blood samples were collected to measure serum creatinine(SCr), blood urea nitrogen(BUN). Kidneys were removed rapidly, excised and sectioned for histological analysis. Other kiney tissues were utilized for biochemical analysis. RESULTS: Quercetin prevented urinary protein, SCr, BUN, NAG, and kidneys histological alterations.Quercetin significantly decreased malondialdehyde(MDA) content while significantly increased glutathione(GSH) content and glutathione-S-transferase(GST), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), catalase(CAT) activities of kidneys tissues in the rats treated with CsA. CONCLUSION: Quercetin can effectively attenuate the nephrotoxicity induced by CsA.