OBJECTIVETo study the effect of light intensity on physiological and biochemical characteristics of Chrysanthemum morifolium at the vegetative stage.

METHODThe dynamic response of physiological and biochemical indexes of Ch. morifolium were measured under different treatments (100%, 80%, 60%, 40% and 20% of the full sunlight) at the vegetative stage.

RESULTThe physiological and biochemical indexes of Ch. morifolium showed dynamic changes with the progress of growth and the increase of the treatment time. The soluble sugar content decreased when the light intensity reduced, and had a significant positive correlation with the light intensity. Soluble protein content rose firstly and fell later, malondialdehyde content increased, superoxide dismutase and catalase activity decreased initially and increased afterwards.

CONCLUSIONProper shading benefits the nitrogen accumulation of Ch. morifolium at the vegetative stage, and reduces the strength of stress condition. The suitable light environment for growth of Ch. morifolium at the vegetative stage is about 80%-60% of full sunlight and the optimum treatment time is 20-40 days.

Catalase ; metabolism ; Chrysanthemum ; physiology ; Light ; Lipid Peroxidation ; Superoxide Dismutase ; metabolism

Ferroptosis is a type of regulated cell death driven by iron-dependent lipid peroxidation that has received extensive attention in recent years. A growing body of evidence suggests that ferroptosis contributes to the progression of drug-induced liver injury. Therefore, the role and mechanism of ferroptosis in the process of drug-induced liver injury deserve further extensive and in-depth exploration, which will aid in the discovery of novel biomarkers as well as the identification of potential approches of targeting ferroptosis to intervene in drug-induced liver injury.
Humans ; Biomarkers/metabolism* ; Chemical and Drug Induced Liver Injury ; Ferroptosis ; Iron/metabolism* ; Lipid Peroxidation/physiology*

Extracellular Matrix ; physiology ; Humans ; Lipid Peroxidation ; Liver ; pathology ; Liver Cirrhosis ; etiology ; Receptors, Retinoic Acid ; physiology ; Retinoid X Receptors ; Risk Factors ; Sodium-Hydrogen Exchangers ; physiology ; Transcription Factors ; physiology

AIMTo investigate the effects of CoQlo supplementation on liver mitochondrial function and aerobic capacity in adolescent athletes.

METHODSBased on a single blinded study design, 18 male adolescent swimming athletes were randomized into two groups, supplement CoQ10 100 mg/d (Q group), or placebo (P group) for 28 days respectively.

RESULTS(1) After supplementation, the plasma CoQ10 concentration in Q group was significantly elevated and significantly higher compared to P group. (2) After supplementation, the rest plasma MDA level in Q group remained unchanged and was significantly lower compared to P group. (3) The plasma CoQ10 concentration of the 18 athletes was significantly decreased during the first constant endurance exercise. (4) The baseline plasma CoQ10 of the 18 subjects showed significantly positive correlation with VO2max measured in the first incremental exercise. (5) No significant difference of increased level of AKBR between Q group and P group. (6) No significant difference of increase level of VO2max, individual lactate threshold and exercise economy between Q and P group.

CONCLUSIONAlthough there is an increased demand for plasma CoQ10 during endurance exercise and CoQ10 supplement can depress lipid peroxidation, there is no effect of CoQ10 supplementation on liver mitochondrial function and aerobic capacity in adolescent athletes.

Adolescent ; Exercise ; Humans ; Lipid Peroxidation ; Male ; Mitochondria, Liver ; metabolism ; physiology ; Physical Endurance ; Swimming ; Ubiquinone ; administration & dosage ; analogs & derivatives

OBJECTIVESTo evaluate the effect of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSHPx) on germ cell apoptosis in cryptorchidism.

METHODSForty-eight immature male Sprague-Dawley rats were divided into the cryptorchid and the control group randomly. Testes were harvested at 1, 3 and 7 days after the operation. Germ cell apoptosis was determined by TUNEL technique, at the same time the activities of antioxidant enzymes and the level of malonic diethyl aldehyde (MDA) were detected with spectrophotometer.

RESULTSAt the 7th day, as compared with the control, testicular weight, SOD activities, CAT activities and the ratio of SOD/(CAT + GSHPx) were reduced significantly(P < 0.01), GSHPx activities had no apparent change(P > 0.05), while apoptosis index and the MDA level increased predominantly in cryptorchidism(P < 0.01).

CONCLUSIONSGerm cell apoptosis in cryptorchidism related closely to the decrease of antioxidant enzyme activity.

Animals ; Apoptosis ; Catalase ; physiology ; Cryptorchidism ; pathology ; Glutathione Peroxidase ; physiology ; Lipid Peroxidation ; Male ; Organ Size ; Rats ; Rats, Sprague-Dawley ; Spermatozoa ; pathology ; Superoxide Dismutase ; physiology

Animals ; Female ; Hydroxyl Radical ; metabolism ; Lipid Peroxidation ; physiology ; Liver ; metabolism ; Male ; Physical Conditioning, Animal ; physiology ; Random Allocation ; Rats ; Rats, Sprague-Dawley ; Sex Factors ; Time Factors

OBJECTIVETo study the role of lipid peroxidation injury and endoplasmic reticulum stress in Al-induced apoptosis.

METHODSNeurons from 0-3 day rats were cultured and treated with different concentrations of AlCl3.6H2O. Morphologic changes of neurons and endoplasmic reticulum were observed under fluorescent and transmission electron microscope; activities of superoxide dismutase (SOD), malondialdehyde (MDA) and ATP enzymes were detected.

RESULTSTypical morphologic changes in neurons apoptosis and endoplasmic reticulum were found under fluorescent and transmission electron microscope; SOD enzyme viability and ATP enzyme viability were significantly increased in the low-dosage group, but reduced in mid and high-dosage group (P < 0.01), whereas MDA levels decreased in the low-dosage group, but increased in mid and high-dosage group (P < 0.01).

CONCLUSIONAluminum may induce neurons apoptosis, and lipid peroxidation injury in endoplasmic reticulum plays an important role in the apoptosis progression.

Aluminum ; toxicity ; Animals ; Apoptosis ; drug effects ; Cells, Cultured ; Endoplasmic Reticulum Stress ; physiology ; Lipid Peroxidation ; physiology ; Neurons ; drug effects ; metabolism ; pathology ; Rats ; Rats, Sprague-Dawley

OBJECTIVETo investigate oxidative stress, hemoglobin percentage and erythrocyte osmotic fragility in various aging groups.

METHODSA total of 200 healthy volunteers of both genders between age group 20-65 years were selected by random method. Determination of hemoglobin percentage was done employing modified cyanide method of Dacie and Lewis. The erythrocyte lysis was observed in hypotonic solution of buffered saline at varying concentrations and optical density was measured at 540 nm. The extent of lipid peroxidation in form of malondialdehyde was measured by thiobarbituric acid method.

RESULTSThe study found a significant decrease in hemoglobin percentage, increase in erythrocyte osmotic fragility and increased lipid peroxidation in form of malondialdehyde with increasing age.

CONCLUSIONSSupplementation of antioxidants may prevent the oxidative injury in elderly group of subjects.

Adult ; Aged ; Aging ; physiology ; Erythrocytes ; chemistry ; Female ; Hemoglobins ; analysis ; Humans ; Lipid Peroxidation ; physiology ; Male ; Malondialdehyde ; analysis ; metabolism ; Middle Aged ; Osmotic Fragility ; Oxidative Stress ; Young Adult

AIMTo investigate if interleukin-6 (IL-6), interleukin-8 (IL-8), interleukin-10 (IL-10), interferon-gamma (IFN-gamma) or tumor necrosis factor-alpha (TNF-alpha) are able to stimulate the level of lipid peroxidation of sperm membranes, either alone or in the presence of leukocytes.

METHODSSemen samples from normozoospermic donors were prepared by density gradient. The sperms were exposed to the indicated cytokines, at physiological and infection-inflammation concentrations, in the absence or presence of leukocytes. Lipid peroxidation of the sperm membranes was determined by measuring malondialdehyde (MDA) and 4-hydroxialkenals (HAE) formation.

RESULTSTNF-alpha, IL-8 and IFN-gamma increased the level of sperm membrane lipid peroxidation when tested at physiological concentrations. At infection-inflammation concentrations, only IL-8 was able to produce a higher effect. When assayed in the presence of leucocytes, IL-8 and TNF-alpha showed a higher effect at infection-inflammation concentrations than at physiological concentrations. Finally, IL-8 showed a higher effect in the presence of leukocytes than in their absence at both physiological and infection-inflammation concentrations. TNF-alpha also showed a higher effect when assayed in the presence of leukocytes than in their absence, but only at infection-inflammation concentrations. There was no effect of IL-6 or IL-10 in any of the tested conditions.

CONCLUSIONSeveral pro-inflammatory cytokines at physiological concentrations increase the level of lipid peroxidation of sperm membranes, which could be important for the sperm fecundation process. However, infection-inflammation concentrations of some cytokines, such as IL-8 and TNF-alpha, either alone or in the presence of leukocytes, could drive the lipid peroxidation of the spermatozoa plasma membrane to levels that can affect the sperm fertility capacity.

Cytokines ; analysis ; Humans ; Inflammation ; physiopathology ; Interferon-gamma ; metabolism ; Interleukins ; metabolism ; Leukocyte Count ; Lipid Peroxidation ; Male ; Reference Values ; Spermatozoa ; physiology ; Tumor Necrosis Factor-alpha ; metabolism

OBJECTIVETo investigate the effect of interleukin-2 (IL-2) on myocardial impairment during ischemia/reperfusion or anoxia/reoxygenation.

METHODSChemical anoxia was introduced in the isolated rat ventricular myocytes by Krebs-Henseleit (K-H) solution containing 10(-3) mol/L sodium dithionite. The video-tracking system and spectrofluorometric method were employed to verify the cell contraction and calcium homeostasis of the single myocyte. Radioimmunoassay was used to analyze the IL-2 levels in myocardium.

RESULTSThe levels of IL-2 in myocardium subjected to ischemia/reperfusion were elevated [(14.34+/-5.99 compared with 22.25+/-3.68)ng/g, P<0.01]. During anoxia, cell contraction and the amplitude of electrically induced calcium transient were depressed and the parameters did not return to the pre-anoxia level during reoxygenation. IL-2 at 200 U/L administered during anoxia aggravated the effect of reoxygenation on cell contraction and calcium transient. After perfusion with IL-2, the malondialdehyde content of myocardial mitochondria was elevated.

CONCLUSIONCoexistence of IL-2 during anoxia aggravates the effect of reoxygenation on the cell contraction and calcium homeostasis in the isolated rat ventricular myocytes, in which the mitochondrial lipid peroxidation induced by IL-2 is involved.

Animals ; Calcium ; metabolism ; Interleukin-2 ; physiology ; Lipid Peroxidation ; Male ; Malondialdehyde ; blood ; Mitochondria, Heart ; metabolism ; Myocardial Ischemia ; physiopathology ; Myocardial Reperfusion Injury ; physiopathology ; Rats ; Rats, Sprague-Dawley