Objective To establish a HPLC fingerprint identification method for the stir-frying product of Selaginella moellendorffii Hieron.Methods RP-HPLC method was performed on Zorbax C18(250 mm? 4.6 mm,5 ? m)column.The chromatographic condition was as follows:mobile phase consisting of 0.5 % acetic acid and acetonitrile,gradient elution,flow rate of 1 mL/min and detection wavelength at 260 nm and 338 nm.Results There were 12 feature peaks,which made up HPLC fingerprint pattern of stir-frying products of Selaginella moellendorffii Hieron.Conclusion The method is simple,accurate with a good repeatability,and can be used for the quality control of stir-frying product of Selaginella moellendorffii Hieron.

Objective:To search the effects and possible mechanisms of Lianggesan on the acute lung injury(ALI) rat induced by endotoxin. Methods: Endotoxemia was induced by intravenous lipopolysaccharide (LPS,5 mg/kg), then treated with Lianggesan. The levels of TNF-?, IL-1?and IL-10 in serum and wet-to-dry weight ratio of lung were detected. Results:In LPS group the levels of TNF-? began to increase at 1h, which reached the peaks at 2h(P

Objective To investigate the molecular mechanism of inflamation development and anti-inflamation response of Lianggesan Decoction(Decoction for cooling the chest) on endotoxin-induced acute lung injury rats. Methods The rat models with acute lung injury were made by lipopolysaccharide(LPS) through intravenous injection.The high,middle and low dosage of Lianggesan Decoction were given by gastric perfusion and compared with dexamethasone(DXM).The contents of Th1 and Th2 in peripheral blood and bronchoalveolar lavage fluid(BALF) were measured at different time points to observe the changes in Th1/Th2.Results In the model group,Th1/Th2 in peripheral blood and BALF was gradually lowering,the difference was significant compared with those of the blank group(P

Objective To select the optimal exacting process for Chuanping prescription (CP).Methods The anti- and the anti- asthma ticanti effects of extracts obtained by different extracting processes from CP were observed.Results The anti- asthmatic effect of extract obtained by ion exchange resin(IER) method was better than that by acid- liquid- extraction alcohol- precipitate method (ALEAP) (P

AIM: To investigate the action of Xingshen Capsule(Tea, Radix Ginseng, etc.) on anti-centre and body fatigue in mice. METHODS: Mice were divided into five groups and were given different dosage once a day for six days, then, their weight, motor activity, roating bar tolerance and the hypnogenetic effects of sodium pentobarbital were observed on the 1 st and 6 th day. RESULTS: Comparing with the negative control, Xingshen Capsule could notably increase the times of motor activity, prolong the persisting time on the roating bar, shorten the sleeping time caused by sodium pentobarbital while prolong its latence. CONCLUSION: Xingshen Capsule may have outstanding effect on refreshing one's nerve and body.

AIM: To evaluate the main pharmacological effect of Wunzhonganwei Soft Capsule. METHODS: Analgesic effect was investigated by hot plate test and writhing test, anti-inflammation effect by auricular swelling test in mice and toe swelling test in rat, anti-diarrhea effect by diarrhea test induced by rhubarb, effect on gastric emptying by phenolsulfonphthalein empty test in mice and effect on small intestine propulsive test by charcol powder propulsive rate test. RESULTS: Wunzhonganwei Soft Capsule enhanced thermal stimulation threshold in mice, decreased the occurrence of writhing caused by glacial acetic acid in mice, inhabited xylene-induced auricular swelling in mice and carrageenan-induced toe swelling in rat, decreased the number of loose stools induced by rhubarb in mice., inhabited the function of gastric emptying induced by metoclopramide or in normal mice, antagonized the inhibitory effect of gastric emptying induced by atropine and inhabited small intestine propulsive movement induced by neostigmineor or in normal mice. CONCLUSION: Wunzhonganwei Soft Capsule has the effect of anti-inflammation, analgesia, antidiarrhea and adjusting the function of gastrointestinal movement.

Objective To develop a gas chromatography-mass spectrometry (GC-MS) method for the determination of ephedrine (E) and pseudoephdrine (PE) and to study the pharmacokinetics features of E and PE in healthy human volunteers. Methods The serum concentrations of E and PE were measured by GC-MS. The pharmacokinetic parameters were computed by using WinNonlin program. Results A good linearity was obtained in E from 5 ng/mL to 1000 ng/mL (r2=0.9985) and in PE from 2.5 ng/mL to 500 ng/mL (r2=0.9994). The recoveries were in the range of 107.5 %to 96.2 %and 102.5 %to 93.9 %for E and PE respectively. The interday RSD and intraday RSD of E and PE were both less than 5 %. The E and PE plasma solution were steady within 60 days. Conclusion This GC-MS method is a accurate and reliable method for the determination of E and PE in human plasma.

Object To explore the regularity of recipe composition by observing different bacteriostasis among compositions of Gegen Qinlian Decoction (GQD) against Streptococcus pneumoniae. MethodsTo evaluate the minimal inhibitory concentration (MIC) of 15 decoctions through double fold dilution method. Results Not all of the decoctions are of the same MIC. Conclusion Not all of the 15 decoctions have the same bacteriostasis against S. pneumoniae. Among the four herbs of GQD Coptis chinensis Franch. has the strongest bacteriostasis and the next Scutellaria baicalensis Georgi. While Glycyrrhina wralensis Fisch and Pueraria lobata (Willd.) Ohwi have no bacteriostasis, but they can influence the bacteriostasis of C. chinensis and S. baicalensis when combinating with them.

AIM: To investigate combination principle of Gegenqinlian Decoction through experiment of anti-diarrhea. METHODS: Propelling rate of mouse's small intestine was selected as index on the condition of ink as colour reagent, diarrhea frequence served as second index for orthoganal design on the basis of mouse's diarrhea induced by senna. RESULTS:Coptis chinensis franch and Glycyrrhiza uralensis fisch and reduce drive rate. Scutellaria baicalensis Georgi and Pueraria lobata(wild) ohwi had counteracter against Coptis chinensis franch's effect, while Glycyrrhiza uralensis fisch could enforce Coptis chinensis franch's effect.as to DI, Coptis chinensis franch had anti-diarrhea effect. Scutellaria baicalensis Georgi had counteracter against Coptis chinensis franch's effect, Pueraria lobata(wild) Ohwi could enforce Scutellari, baicalensis Georgi effect, but Glycyrrhiza uralensis fisch could enforce Coptis chinensis's effect after compounding with Scutellaria baicalensis Georgi. CONCLUSION: ~Among the combinantion of Gegenginlian Decoction, as to the pharmcological effects on anti-diarrhea, the best compound is Coptis chinensis franch and Glycyrrhiza uralensis fisch.

OBJECTIVE:To observe the efficacy and economy of omeprazole and esomeprazole in the treatment of brain trauma and cerebral hemorrhage complicated with upper gastrointestinal hemorrhage. METHODS:The data of 110 patients with trauma cere-bral hemorrhage complicated with upper gastrointestinal hemorrhage were retrospectively analyzed and divided into omeprazole group (56 cases)and esomeprazole group(54 cases). All patients were given conventional treatment. On this basis,omeprazole group was treated with Omeprazole for injection 40 mg by intravenous infusion;esomeprazole group was treated with Esomeprazole injection 40 mg by intravenous infusion,twice a day. The treatment course for 2 groups was 5 d. The efficacy and economy of patients were com-pared. RESULTS:The total effective rates in esomeprazole group were significantly higher than omeprazole group higher,the cost-ef-fectiveness in esomeprazde group(1 397.71)were significantly lower than omeprazole group(1 512.09)(P<0.05),andΔC/ΔE=91.52. CONCLUSIONS:Esomeprazole has good efficacy,safety and economy in the treatment of brain trauma cerebral hemorrhage compli-cated with upper gastrointestinal hemorrhage.