1.Research progress on the relationship between social isolation and cognitive ability in the elderly
Song GAO ; Lili WANG ; Wanlu LIU ; Liduo FAN ; Yanli XIU ; Linyu BI
Chinese Journal of Geriatrics 2023;42(5):603-608
Social isolation represents the development of a certain level, either partial or complete, of deprivation of socialization and may have adverse effects in many aspects for the elderly, which can be physiological, psychological and social.Meanwhile, during the course of human life, aging becomes an inevitable process and brings about changes in cognitive ability, which become an important focus of our attention.This paper reviews the research progress on the relationship between social isolation and cognitive ability in the elderly, in order to provide a new perspective for future research on social isolation and cognitive ability in the elderly and also to offer new insight on how to construct a model of intervention and health management for the elderly population with social isolation.
2.Prokaryotic expression, purification, refolding and biological assays of recombinant human interleukin 4 inclusion body.
Jiong LI ; Kaijun CUI ; Jing WEN ; Zhiwei ZHAO ; Ping CHEN ; Ling TIAN ; Bing KAN ; Yanjun WEN ; Hongxin DENG ; Linyu FAN ; Yuquan WEI
Journal of Biomedical Engineering 2007;24(4):866-869
A DNA fragment encoding human interleukin 4 was obtained by PCR from pORF-hIL4 plasmid. The amplified fragment was inserted into prokaryotic expression vector PQE60 and recombinant protein was expressed in E. Coli M15 by adding isopropyl-beta-D-thiogalactoside (IPTG). The hIL-4 protein was present as insoluble inclusion bodies in the bacterial extract. After denaturation of inclusion bodies with 5 mol/L guanidine hydrochloride, the supernate was diluted to get renaturized. Then dialysis and Ni chelating chromatography were used for purification. TF-1 proliferation assay of recombinant human interleukin 4 was performed, and then rhIL-4 was fit to be used for proliferation of human dendritic cells from monocyte in vitro.
Escherichia coli
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genetics
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metabolism
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Genetic Vectors
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Humans
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Inclusion Bodies
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metabolism
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Interleukin-4
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biosynthesis
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genetics
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Protein Folding
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Recombinant Proteins
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biosynthesis
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genetics
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isolation & purification