OBJECTIVETo develop an HPLC method for determining four components in Sini Tan, benzoylmesaconine, liquiritin, glycyrrhizic acid and 6-gingerol.

METHODThe Hypersil BDS column was adopted with gradient elution program at a flow rate of 1.0 mL x min(-1) and the detection wavelength of 235 nm.

RESULTBenzoylmesaconine, liquiritin, glycyrrhizic acid and 6-gingerol showed good separation, with the linear range of 0.006-0.12, 0.021-0.42, 0.012-0.24 and 0.018-0.36 g x L(-1), respectively. Their average recoveries were 99.3%, 96.9%, 100% and 100%, respectively; and RSD of the above four components were 1.5%, 0.6%, 1.3% and 2.1%, respectively.

CONCLUSIONThe method is proved to be so easy and accurate and practical that it can be used to determine the four components in Sini Tang.

Aconitine ; analogs & derivatives ; analysis ; isolation & purification ; Anti-Inflammatory Agents ; analysis ; isolation & purification ; Catechols ; analysis ; isolation & purification ; Chromatography, High Pressure Liquid ; methods ; Drugs, Chinese Herbal ; chemistry ; Fatty Alcohols ; analysis ; isolation & purification ; Flavanones ; analysis ; isolation & purification ; Glucosides ; analysis ; isolation & purification ; Glycyrrhizic Acid ; analysis ; isolation & purification ; Medicine, Chinese Traditional ; Reproducibility of Results