Objective:To explore the clinical significance of the expression of nuclear factor of activated T cells 5 (NFAT5) in esophageal cancer tissues and the effect of the expression of knock-down esophageal cancer cells on their migration ability.Methods:The expression of NFAT5 in tissues of 26 patients with esophageal cancer and their adjacent tissues was detected by immunohistochemistry. Esophageal cancer cells ECA109 were divided into experimental group and control group. The experimental group ECA109 cells were transfected with NFAT5-siRNA plasmid, and the control group ECA109 cells were transfected with MOCK-siRNA plasmid. The mRNA content of NFAT5 was detected by fluorescence quantitative PCR. The expression of NFAT5, TLR4 and MyD88 proteins in the experimental group and control group were detected by Western blot. Transwell assay and cell scratch assay were used to detect the migration ability of the experimental group and the NC group.Results:Immunohistochemical test results showed that the positive rate of NFAT5 in esophageal cancer tissues was 80.77% (21 cases/26 cases) , and the expression rate was 15.38% (4 cases/26 cases) in corresponding adjacent tissues. The positive rate of NFAT5 protein in esophageal cancer tissues was significantly higher than that in adjacent tissues ( P<0.001) . The NFAT5 mRNA content of ECA109 cells in experimental group and control group decreased after transfection with corresponding siRNA. The protein expression levels of NFAT5, TLR4 and MyD88 in ECA109 cells in the experimental group were 0.28±0.08, 0.31±0.13 and 0.41±0.14, respectively. The protein expression levels of NFAT5, TLR4 and MyD88 in ECA109 cells in control group were 0.95±0.15, 0.84±0.22 and 1.04±0.26, respectively. The expression of TLR4 and MyD88 in esophageal cancer ECA109 cells decreased significantly ( P<0.05) . The scratch healing rate of esophageal cancer ECA109 cells was 52.67%±5.21% in the experimental group and 82.91%±7.26 % in the control group. Transwell experiment results showed that the number of successfully migrated cells in the experimental group was (35±5) , and the number of successfully migrated cells in the control group was (92±13) . The results showed that the migration ability of esophageal cancer ECA109 cells was significantly decreased after low expression of NFAT5 ( P<0.01) . Conclusions:The expression of NFAT5 is significantly increased in esophageal cancer tissues, and the expression of NFAT5 may be related to the malignant degree of esophageal cancer. Moreover, NFAT5 affects the migration ability of esophageal cancer cells by regulating TLR4/MyD88 signaling pathway.

BACKGROUND:The stability of the pelvis is mainly determined by the posterior pelvic ring and the sacroiliac joint.The posterior pelvic ring injury and the dislocation of the sacroiliac joint caused by high energy impacts such as car accidents increase year by year.Surgical treatment is the best method,and there are many kinds of endophytorepair methods in clinical practice,but which treatment method has the best biomechanical properties is still controversial. OBJECTIVE:To compare the biomechanical properties of three kinds of internal implants:anterior double plates,posterior bridging plate and tension nail in the repair of unilateral unstable pelvic posterior ring injury,to provide a reference for the clinical treatment and development of a new pelvic tension screw. METHODS:(1)Finite element simulation:Mimics,Wrap and SolidWorks were used to establish normal pelvic model,unilateral injured pelvis model,and three kinds of internal implant repaired models(anterior double plates,posterior bridging plate and tension nail).Ansys was used to analyze the stress and deformation of the models.(2)Biomechanical test:A total of 15 intact pelvic specimens were randomly grouped into five groups,normal pelvic model,unilateral injured pelvis model,anterior double plates,posterior bridging plate and tension nail groups.The mechanical test was performed using an Instron E10000 testing machine. RESULTS AND CONCLUSION:(1)Simulation:In the normal pelvic model,the average displacement of the sacrum was 0.174 mm,and the maximum stress of the sacral iliac bone was 10.51 MPa,and the stress distribution was uniform.The mean sacral displacement of the unilateral injured pelvis model was 0.267 mm,and the stress concentration of the model was obvious.The mean displacement of the sacrum in the three repaired models was close to that in the normal pelvic model,and the stress distribution of the sacral iliac bone in the tension nail repaired model was uniform.(2)Mechanical test:The stiffness of the normal pelvic model was(226.38±4.18)N/mm,and that of the unilateral unstable pelvic model was the smallest(130.02±2.19)N/mm.The deviation of the normal pelvic model stiffness and the three repaired models'stiffness were all within(±10%),and the repair effect was obvious.(3)The simulation results were in agreement with the experimental results.(4)The biomechanics of the tension nail repaired model was the most similar to that of the normal pelvis,and this method was the best.The repairing stiffness of the anterior double plate was too large,and the stress shielding effect was more significant.The posterior bridging plate repair could not solve the compensatory effect of the normal side soft tissue and had defects.This study provides an optimal basis for clinical surgery.(5)The new type of pelvic tension nail should be improved from the point of view of the tension nail to retain the good biomechanical properties of the tension nail,while adding other advantages,such as being used for the osteoporotic pelvis.