Aim To explore the effect of atorvastatin on NF-?B/I?B signal pathway,P selectin and ICAM-1 expression in cholesterol-rich diet-induced rabbit atherosclerosis.Methods 24 New Zealand white rabbits were randomly divided into three groups: control group,high-cholesterol group and atorvastatin group.Nucleus NF-?B p65 and cytoplasma I?B? were detected with Western blot technique.Plasma P selectin was detected with Immunoradioassay.Immunohistochemistry was employed to detect the expression of ICAM-1.Results Compared with the control group,the expression of nucleus NF-?B p65,P selectin and ICAM-1 increased significantly and cytoplasma I?B? reduced in high-cholesterol group(P

Type 2 diabetes mellitus(T2DM)is a complicated endocrine disease caused by both genetic and environmental factors,many of which are unknown as far as for the pathogenesis of T2DM is concerned. In recent years,diabetes researchers have paid more attention to the intestinal microbiota. The discovery,verification and molecular mechanism of the intestinal microbiota related to T2DM are the focus of this article. The role of intestinal microecology in the development of T2DM is also reviewed,involving diet,chronic inflammation,short chain fatty acids,bile acids,the intestinal microbiota and circadian rhythm,and microRNA.

Although there are plenty of principles and standards on translation of traditional Chinese medicine into English, still mistranslation can not be completely avoided. This paper analyzed the phenomenon of mistranslation from perspective of its causes, cases and suggestion in order to provide enlightenment on improving translation levels.

Objective To compare the overall diagnostic accuracy of MRCP with EUS for the detection of choledocholithiasis and malignant obstruction in patients with suspected biliary obstruction.Methods A fully recursive literature search was conducted in The Cochrane Central Register of Controlled Trials CENTRAL,MEDLINE or PUBME (1980-2012),EMBASE (1980-2012),OVID Database (1980-2012),CBM (1980-2012),VIP database (1989-2012),Chinese journal of full-text database (CNKI) (1980-2012),and WANFANG database(1980-2012).The prospective diagnostic studies which evaluated or compared the diagnostic accuracy of MRCP and EUS were included combined with manual searches.We also searched the references of all included articles of important meetings and journals.QUA-DAS items were used to evaluate the quality of the included studies.SEN,SPE,+ LR,-LR and the areas under SROC were detected,then t-test was used to evaluate whether statistically significant difference existed between EUS and MRCP.Results Thirteen studies including 1200 cases were recruited.The overall pooled sensitivities of MRCP and EUS for the detection of choledocholithiasis were 0.870 (95％ CI:0.826-0.906)and 0.935 (95％ CI:0.90-0.96) respectively,whereas their specificities were 0.952 (95％ CI:0.926-0.971) and 0.947 (95％ CI:0.920-0.967),respectively.The overall pooled positive likelihood ratio for MRCP and EUS were 14.055 (95％ CI:6.259-31.561) and 16.653 (95％ CI:6.896-40.212),respectively,with the corresponding negative likelihood ratio of 0.177 (95％ CI:0.108-0.290) and 0.076 (95％ CI:0.049-0.118),respectively.Areas under the ROC curve were 0.9693 and 0.9771,respectively.There were no statistically significant difference for sensitivity (0.84 ± 0.16 vs 0.93 ± 0.07,P =0.108) and specificity (0.93 ±0.10 vs 0.90 ±0.15,P =0.555) between MRCP and EUS.The overall pooled sensitivities of EUS and MRCP for the detection of malignancy were 0.959 (95％ CI:0.908-0.987),and 0.805 (95％ CI:0.724-0.871),respectively,whereas their specificities were 0.975 (95％ CI:0.954-0.988) and 0.927 (95％ CI:0.897-0.951),respectively.The overall pooled positive likelihood ratio for EUS and MRCP were 23.398 (95％ CI:12.987-42.155) and 13.448 (95％ CI:4.961-36.456),respectively,with the corresponding negative likelihood ratio of 0.059 (95％ CI:0.028-0.122) and 0.134(95％ CI:0.046-0.391),respectively.Areas under the ROC curve were 0.9870 and 0.9686,respectively.There were no statistically significant differences for sensitivity (0.95 ± 0.55 vs 0.88 ± 0.14,P =0.22) and specificity (0.96 ±0.03 vs 0.91 ±0.11,P =0.31) between MRCP and EUS.Conclusion MRCP and EUS are both valuable in differential diagnosis of biliary obstruction caused by choledocholithiasis or malignant lesions.

Objective To investigate the protective effects of polysaccharide from Radix Codonopsis Lanceolatae on acute alcoholic hepatic injury in mice.Methods The model of CCl4-induced acute hepatic injury was set up in mice, and then treated with polysaccharide from Radix Codonopsis Lanceolatae to observe the activities of ALT, AST and TG in serum and the levels of SOD, GSH and MDA in hepatic tissue and Observed the pathological changes in the liver of mice.Results Compared with model control group, the lance asiabell root polysaccharide liver injury in mice all dose groups in the activity of serum ALT(97.5 ± 22.12 U/L, 108.8 ± 22.15 U/L, 125.2 ± 19.68 U/Lvs. 152.3 ± 17.32 U/L), AST (72.5 ± 21.3 U/L, 89.2 ± 17.47 U/L, 104.3 ± 19.34 U/Lvs.1215 ± 22.31 U/L) and TG(0.75 ± 0.32 mmol/L, 0.80 ± 0.22 mmol/L, 0.96 ± 0.21 mmol/L vs.1.44 ± 0.23 mmol/L) reduce, can increase the liver damage the activity of SOD(215.6 ± 22.68 U/mg, 203.2 ± 22.18 U/mg, 186.2 ± 18.34 U/mgvs.164.3 ± 17.54 U/mg), GSH(17.74 ± 2.12μmol/g, 14.84 ± 1.12μmol/g, 13.93 ± 2.15μmol/gvs.12.23 ± 2.09μmol/g) in liver tissue of mice, decrease the activity of MDA(6.22 ± 2.12 nmol/mg, 6.22 ± 2.12 nmol/mg, 8.36 ± 1.84 nmol/mgvs.9.35 ± 1.68 nmol/mg) in liver tissue, showing significant differences (P<0.05), and there was certain dose dependent, and can improve the pathological injury of liver tissue.Conclusion Polysaccharide from Radix Codonopsis Lanceolatae had protective effects on acute liver induced by acute alcoholic hepatic injury in mice.

Objective To evaluate the function imaging ability and dose for whole organ imaging of Aquilion ONE 320 Slice CT.Methods Aquilion ONE 320 Slice CT adpoted wide volume imaging mode to acquire 4D dynamic imaging results,then the expose dose was compared with that of 64 slice CT.Results 320 slice CT,with advantages in dynamic volume imaging and function imaging when compared with traditional spiral CT,restrained its organ time resolution to 0.35s,and thus the acquisition time and exposure dose was decreased.The motion artifact was reduced and image quality was enhanced.Conclusion 320 slice dynamic volume CT,with zero phase delay,can realize CT 4D function imaging.

OBJECTIVE:To study the effects of total glucosides of Verbena officinalis on chronic nonbacterial prostatitis (CNP)in mice and its anti-inflammatory and analgesic effects. METHODS:60 mice were randomized into normal group(normal saline),model group(normal saline),positive group(Qianliekang tablet 1.5 g/kg)and total glucosides of V. officinalis high-dose, medium-dose and low-dose groups(0.2,0.1,0.05 g/kg),with 10 mice in each group. CNP model was induced in those groups ex-cept for normal group;mice were given relevant medicine once a day for consecutive 21 days since 8th day of modeling. The white blood cell count,lecithin corpuscle density and pathological changes of prostate samples were observed. 50 mice were randomly di-vided into model group(normal saline),positive group(Aspirin tablet 0.3 g/kg or Dexamethasone acetate tablet 0.01 g/kg)and to-tal glucosides of V. officinalis high-dose,medium-dose and low-dose groups(0.2,0.1,0.05 g/kg);they were given relevant medi-cine intragastrically once a day for consecutive 7 days. The xylene-induced ear swelling test (Aspirin tablet as positive drug) and cotton-pellet granuloma test (Dexamethasone acetate tablet as positive drug) were conducted to determine the the degree of ear swelling and the net weight of granuloma. The acetic acid writhing test and hot plate test(Aspirin tablet as positive drug)were car-ried out to determine the latency period of writhing,the times of writhing within 20 min and pain threshold. RESULTS:Compared with model group,white blood cells count of prostate tissue were lowered while the density of lecithin corpuscles were increased significantly in medicine groups;the pathology of prostate tissue were improved significantly in positive group and total glucosides of V. officinalis high-dose and medium-dose groups,and mostly were 0 and Ⅰ grade. The degree of ear swelling and the net weight of granuloma were markedly reduced in positive group and total glucosides of V. officinalis high-dose and medium-dose groups;the times of writhing within 20 min decreased in medicine groups,and the latency period of writhing prolonged and pain threshold in-creased in positive group and total glucosides of V. officinalis high-dose group. All of the results showed statistical difference(P<0.05 or P<0.01). CONCLUSIONS:Total glucosides of V. officinalis can improve CNP in mice,and shows good anti-inflammatory and analgesic effects.

Objective An investigation on social function cognition for bedside care service at patient's home was conducted in the residents aged over SS years in Jingshan Community,Dongcheng District,Beijing,to lay a scientific basis for provision of it.Methods Ninety-seven residents aged over 55 years were selected from 58 families with systematic sampling in Jingshan Community by a questionnaire interview,including history of illness,income,medical expense,awareness of bedside service at patient's home,etc.Results Totally,98.9 % of the residents suffered from at least one kind of chronic disease,98.9 % of them never received bedside care service at their homes,94.9% of them only knew a little of such kind of health care service,91.85% of them knew some social function of it,and 84.5 % of them did not know the criteria of payment for the service.Conclusions Publicity of bedside care service at patient' s home should be strengthened,focusing on its social function,inexpensiveness,convenience,etc.Function of bedside service should be perfected further,with quality care,to make it an important part of community health care.

Objective To explore the dynamic changes of tracheal stem cells during tracheal regeneration after injury induced by fluorouracil(5-FU) in rats. Methods Extracorporeal tracheal injury(Wistar rats) was induced by 5-FU.ABCG2 expression in tracheal epithelium during the process of regeneration was analyzed by indirect immunofluorescence and Western blotting. Results 1.After treatment with 5-FU for 12 hours,the tracheal epithelium shed and there were ABCG2 positive cells among residual cells in G-0.Three hours after the removal of 5-FU,the tracheal rings were covered with flattened epithelial cells. ABCG2 positive cells increased slightly.Six-nine hours after the removal of 5FU,the epithelial cells changed into cuboidal,correspondingly,the ABCG2 positive cells increased obviously;At 24 hours after the removal of 5-FU,most of the epithelial cells were cuboidal and merged into pieces,but the ABCG2 positive cells decreased obviously;Until 48 hours after the removal of 5-FU,only a few positive cells could be seen with the pseudostratified mucociliary epithelium restored similar to its original mode.There were no detectable ABCG2 positive cells in normal tracheal epithelium.2.Western blotting analysis showed that there were different ABCG2 levels at different times after the removal of 5-FU which in accordance with the change of immunofluorescene.ABCG2 was minimally detected after treatment with 5-FU for 12 hours,reaching a maximal level at 6 hours after the removal of 5-FU,and then decreased over time.Until 48 hours after the removal of 5-FU,very low ABCG2 level was detected.Conclusion The expression of ABCG2 is correlated to the number of tracheal stem cells,suggesting that ABCG2 may serve as a marker for isolating stem cells from tracheal epithelium.

Objective To compare the degree of inflammatory response and changes in pulmonary function in patients undergoing coronary artery bypass grafting (CABG) with or without cardiopulmonary bypass (CPB) Methods Elective CABG was performed in twenty-two patients with CPB (n = 11) or off-pump (n = 11). The patients were premedicated with oral midazolam 7.5 mg and intramuscular morphine 10 mg. Anesthesia was induced with fentanyl 5-10 ?g?kg-1 , midazolam 0.05 mg?kg-1 , etomidate 0.15 mg?kg-1 and rocuronium 0.6 mg?kg-1 and maintained with intermittent i.v. boluses of fentanyl and rocuronium. All patients had pulmonary artery catheter and arterial line placed. ECG, SpO2, PET CO2 , body temperature and urine output were monitored. In CPB group aprotinin was given. The patients were transferred to ICU after operation and mechanically ventilated. Extubation was performed when standard extubation criteria were met. The length of ICU stay and hospitalization were recorded. CI, PVRI, SVRI, PA-aO2 , PaO2/FiO2 and QS/QT were measured and calculated before and at the end of operation and 4, 12, 24, 36 h after operation. Blood samples were taken from radial artery and pulmonary artery (mixed venous blood) before and at the end of operation and 4, 12, 36 h after operation for determination of plasma levels of IL-6, IL-8 and neutrophil elastance, the numbers of WBC and neutrophil, the numbers of WBC and neutrophil segregated in the lungs (the number of WBC/neutrophil in mixed venous blood - the number of WBC/neutrophil in arterial blood) .Results The two groups were comparable with respect to age, sex, duration of anesthesia and surgery and the number of grafts. CI was significantly increased while SVRI significantly decreased after operation as compared to the baseline values before operation. There was no significant difference in CI and SVRI after operation between the two groups. The postoperative PVRI and PA-a O2 were significantly lower while postoperative PaO2/FiO2 was significantly higher in the off-pump group than in the CPB group ( P