OBJECTIVE:To facilitate a high performance operation of drug supply chain so as to provide satisfactory service for patients,the common terminal customers.METHODS:The principle of customer demand driven in drug supply chain and the significance of customer demand driven in drug supply chain management as well as the role hospitals played in the practice of the customer demand driven-based drug supply chain management were analyzed and qualitatively described.RESULTS & CONCLUSIONS:In the environment of buyers' market of drugs,as the terminal customers' envoy plenipotentiary and the best valuable clients in the drug market,hospitals play a key role in back driving drug supply chain's high performance operation.

26%,42%,94% which according to Chinese Pharmacoeia(2005 Edition).CONCLUSIONS: The sustained-release tablets thus prepared is proper in technique and well in vitro release effect.

Objective To study the immunoregulation effect of three kinds of polysaccharides extracted from different tissues of Octopus dollfusi on immunosuppression mice.Methods Two kinds of immunosuppression mice models were built by ip,cyclophosphamide(CY) and sc.hydrocortisone(HC),respectively.The spleen and thymus were weighed and the index of the immune organs was calculated.The white blood cells were counted by automated hematologic analyzer.Results In certain concentrations(20～80 mg?kg-1),the three kinds of polysaccharides could antagonize the atrophy of spleen and thymus caused by CY and HC,and increase the weight of spleen and thymus;The polysaccharides could protect against leukocytopenia induced by CY and HC,and especially in low and middle dose.Conclusion The results suggested that three kinds of polysaccharides extracted from Octopus dollfusi have potent enhancement effect on non-specific immunity for the immunosuppression mice.

OBJECTIVE: To introduce validation to the equipments of self-made preparations in medical institutions.METHODS: The necessity and difficulties for the validation of the equipments of self-made preparations in medical institutions were analyzed from aspects of the specificity of self-made preparations,the status quo of the preparation equipment as well as the requirements on the equipments etc.RESULTS: The scope and the procedures of the validation on the equipments of preparations had been presented.CONCLUSIONS: Medical institutions should adopt suitable methods,approach and validation procedures to develop corresponding validation work for equipments of preparations based on their own condition.

OBJECTIVE:To develop a RP-HPLC method for simultaneous determination of oleanolic acid and ursolic acid in Hedyotis diffusa.METHODS:The optimum HPLC conditions were as follows:Nucledure C 18 column(4.6mm?150mm);mobile phase:acetonitrile-potassium phosphate,pH=7.84(45∶55);detection wavelength:220nm.RESULTS:The average recoveries of oleanolic acid and ursolic acid were(96.79?2.42)%and(97.15?2.33)%respectively.CONCLUSION:The met_ hod could detect oleanolic acid and ursolic acid in Hedyotis diffusa simultaneously.It could be used in the quality control of the herb.

OBJECTIVE:To carry out the ISO9001standard quality control system in the department of pharmacy.METHODS:The procedures and methods of PDCA cycle(Plan-Do-Check-Action)were applied to our department's ISO9001quality authentication standardization.RESULTS:The application of PDCA cycle-based ISO9001standards proved to be successful in the standardization of our department.CONCLUTION:The ISO9001quality authentication following the PDCA cycle is effective.

OBJECTIVE:To investigate the application of knowledge management in pharmaceutical department of hos-pitals.MEHTODS:Different models of knowledge management in the pharmaceutical department of our hospital were sum-marized in recent years.RESULTS:The knowledge management model in our pharmaceutical department has been shaped fundamentally.CONCLUSION:Coping capacity and creativity of pharmaceutical department have been strengthened by knowledge management.

Objective To explore the anti-inflammatory and analgesic effects of Shuangwu numbness-relief decoction. Methods The anti-inflammatory effect was assessed by xylene-induced mouse auricular swelling and peritoneal capillary permeability;and the analgesic effect was assessed by hot plate method and acetic acid-induced twisting method. Results Shuangwu numbness-relief decoction at moderate or high dose had a significant inhibitory effect on mouse auricular swelling, with a significant difference as compared with the control group (P<0.01);the decoction at low , moderate , or high dose group played a marked inhibitory role in the increase in mouse peritoneal capillary permeability (P<0.01). Shuangwu numbness-relief decoction at moderate or high dose effectively reduced the writhing frequency in mice (P < 0.01). The decoction at high dose increased the pain threshold in mice one hour after intragastric administration (P < 0.05), and the decoction at moderate or high dose increased the pain threshold two hours after administration (P < 0.05). Conclusions Shuangwu numbness-relief decoction has an obvious anti-inflammatory and analgesic effect.

OBJECTIVE:To establish a method for the simultaneous contents determination of chloramphenicol and diphenhydr-amine hydrochloride in Chlorobenzene alcohol solution. METHODS:HPLC was performed on the column of Diamonsil C with mo-bile phase of phosphate buffer-acetonitrile(66.5∶33.5,V/V)containing 0.01 mol/L sodium heptane,column temperature was 35 ℃, flow rate was 1 ml/min,detection wavelength was 258 nm for diphenhydramine hydrochloride and 277 nm for chloramphenicol, the injection volume was 10μl. RESULTS:The linear range was 34.8-139.4μg/ml for chloramphenicol(r=0.999 8)and 93.0-930.5μg/ml for diphenhydramine hydrochloride (r=0.999 8);RSDs of precision,stability and reproducibility tests were lower than 2.0%;recoveries were 97.70%-104.02%(RSD=2.2%,n=9)and 96.72%-101.72%(RSD=1.6%,n=9). CONCLUSIONS:The method is specific,accurate with good precision,and suitable for the quality control of Chlorobenzene alcohol solution.

Objective The aim of this study was to investigate the anti-tumor effect on sequential injection of heterogeneic lymphocyte(HL)and autogeneic lymphocyte(AL). Methods The HL was prepared by using CC3HF1 mice as feeders. CB6F1 mice were used as recipients,and Hepa1-6 cells were inoculated into the recepients′groin subcutis. A cryoprecipitate was extracted from mouse plasma by freeze-thaw method to prepare fibrin Glue(FG);FG was combined with HL or AL to be FG-HL or FG-AL. The experimental treatment consisted of two stages. At first stage(15 d),FG-HL were injected on the surface of the tumor-bearing tissue of the recipients as the experimental group,and FG-phosphate buffer saline(FG-PBS)were injected on the surface of the tumor-bearing tissue of the rest recipients as the control group. The immunological factors such as tumor cell killing rate of the spleen lym-phocytes and numbers of lymphocytes,CD8 +T and NK in the two groups were detected,respectively. At later stage(10 d),a part of mice were randomly selected from the experimental and control groups,and the lymphocytes( AL) were used to form FG-AL,which were injected on the surface of tumor-bearing tissues in the rest of mice. Tumors in mice of the two groups were compared for tumor volume and tumor inhibition rate. Results The tumor cell killing rate of AL in the experimental group(26. 70 ± 7. 22) was signifi-cantly higher than that in the control group(5. 70 ± 2. 68)(P<0. 01). Numbers of mouse spleen lymphocytes,CD8 +T cells and NK cells were significantly higher than the corresponding values of the control group(P<0. 05). After the two-stage treatment,the aver-age tumor volume of the experimental group[(1.20 ±0.33)cm3]was significantly smaller than that of control group[(2.05 ±0.37) cm3](P<0. 01). The tumor inhibition rate in the experiment group was 41. 5% when compared to the control group. Conclusion Local injections of FG-HL followed by FG-AL can significantly inhibit the growth of transplanted tumor in mice;it is expected to become an anti-tumor biological therapy.