Objective To explore and analyze the related factors of nosocomial infection in patients after total laparoscopic hysterectomy.Methods A total of 231 patients undergoing total laparoscopic hysterectomy were enrolled in this study and their data were retrospectively analyzed.The related factors such as uterus size,operation duration,intraoperative blood loss,type of disease,drainage tube,hospital duration before surgery,and history of pelvic surgery were analyzed.Results Among the 231 cases undergoing total laparoscopic hysterectomy,23 cases had infection and the infection rate was 9.96％.Univariate analysis showed uterus size,operation duration,intraoperative blood loss,hospital duration before surgery,and history of pelvic surgery had influence on postoperative infection and the difference had statistical significance (P＜0.05),while the difference of age and drainage tube had no statistical significance on postoperative infection (P＞0.05).Conclusion The incidence of infection after laparoscopic hysterectomy is related to uterus size,operation duration,intaoperative blood loss,hospital duration before surgery,and history of pelvic surgery.

Objective To observe serum and callus leptin expression in rats model with fracture and traumatic spinal cord injury (SCI). Methods 72 male SD rats were randomized equally into 4 groups: control, SCI group, fracture group, and fracture/SCI group. Rats were sacrificed at 7, 14, 21 and 28 days after fracture/SCI. Serum leptin was detected by radioimmunoassayat 1, 7, 14, 21 and 28 days, and callus formation was measured radiologically at 14, 21 and 28 days. Callus leptin was analyzed with immunohistochemistry. Results Serum leptin in the fracture group, SCI group and combined fracture/SCI group were all higher than in control group at the 1, 7, 14 and 21 day time-point (P < 0.05). Serum leptin in the combined fracture/SCI group was significantly higher than the fracture group at 7, 14 and 21 days (P < 0.05), and higher than SCI groups at 14 and 21 days after operation (P < 0.05). The percentage of leptin-positive cells in the fracture/SCI callus, and callus volume was significantly higher than the fracture-only group (P < 0.001). Conclusions Leptin expression increases in the recovery process after SCI, and the recovery of fracture becomes sooner.

Objective To investigate the mmu-miR-3475-3P possible participating in biology process and signal pathway.Methods The expression of mmu-miR-3475-3P in the mature mouse heart and in the embryonic mouse heart was measured by qRT-PCR.The target genes were predicted through comprehensively using the common microRNA on-line databases such as TargetScan,miRDB and miRanda,and then the obtained targeted genes were performed the gene function annotation and signal pathway analysis.Results There was significant difference between the expression of mmu-miR-3475-3P in the embryonic mouse heart and the expression in the mature heart.Bioinformatics analysis by using TargetScan,miRDB and MiRanda on miR3475-3P revealed that microRNA was likely to regulate 441 target genes.Conclusion mmu-miR-3475-3P is highly expressed in the embryonic mouse heart.The target genes predicted by mmu-miR-3475-3P are enriched in multiple signal pathways and cellular biological processes.

OBJECTIVETo explore the effect of donations after cardiac death (DCD) kidney transplant performed in northwest China and the measures for management of delayed graft function (DGF).

METHODSIn the period of 2011-2013, a total of 51 families of DCD donor gave their consent to organ donation by signing the informed consent with the help by a Red Cross Organization (ROC) coordinator, and 102 kidneys were retrieved by organ procurement organization (OPO) teams. Ninety-four operations of renal transplantation were carried out in our hospital. All the patients were followed-up and based on the occurrence of DGF after transplantation, they were divided into DGF group and non-DGF group for comparative studies.

RESULTSThe success rate of donation after cardiac death was 29.3%, and the incidence of post-transplantation DGF was 27.7%. The 1-year human/kidney survival rate was 98.9%/95.7%. Within six months after the transplant, the values of eGFR in DGF group were significantly lower and serum creatinine significantly higher than those in non-DGF group (P<0.05), but no significant differences were found between the two groups thereafter (P>0.05). The occurrence of DGF in LifePort mechanical perfusion cohorts was significantly lower than that in the simple cold preservation group (21.5% vs. 41.4%, P<0.05).

CONCLUSIONThe overall effect of DCD kidney transplant is good despite a high incidence of early DGF, and we recommend the use of low-temperature mechanical perfusion for storage and transportation of DCD donor kidney.

Adult ; China ; Death ; Delayed Graft Function ; etiology ; Female ; Humans ; Kidney Transplantation ; Male ; Tissue and Organ Procurement ; statistics & numerical data ; Young Adult

Objective To investigate the related factors for the survival of the patients with pancreatic cancer.Methods A total of 1 620 patients confirmed as pancreatic cancer admitted in Sun Yat-sen Memorial Hospital affiliated with Sun Yat-sen University,Tumor prevention and treatment center affiliated with Sun Yat-sen University and People's Hospital of Guangdong Province from 2004 to 2016 were retrospectively analyzed,and the effects of TNM staging,surgical treatment,palliative chemotherapy and postoperative assisted chemotherapy on the survival of the patients with pancreatic cancer were examined by life table and Log-rank test.Results The median survival time of all 1 620 cases was 7.15 months.The median survival time of TNM stage Ⅰ,Ⅱ,Ⅲ and Ⅳ was 12.50 months,10.12 months,9.56 months and 5.43 months,and there was statistically significant difference (P =0.001).The median survival time of cases who did not undergo surgery was 6.10 months,which of patients who underwent radical surgery was 13.67 months,and the difference was statistically significant (P =0.001).The median survival time of cases without chemotherapy was 5.55 months,which of patients who underwent palliative chemotherapy was 7.58 months,and the difference was statistically significant (P =0.001).The median survival time of cases with pure radical surgery without chemotherapy was 12.38 months,which of patients who underwent adjuvant chemotherapy was 14.50 months,and the difference was no statistically significant (P =0.561).Conclusions Early diagnosis followed closely by radical surgery is the key to prolong the survival of pancreatic cancer patients.And adjuvant chemotherapy for patients who lose surgery opportunity may improve clinical prognosis to a certain extent.

BACKGROUNDPeople's attitude toward organ donation after cardiac death (DCD) has not come to an agreement in different countries and regions. Influenced by the local culture in China for thousands of years, the general public has different ideas about this issue. The purpose of this study was to investigate the current attitudes trend and characteristics of transplantation with organs donated after cardiac death in northwest China.

METHODSThis largest single-center cohort study was performed by an interview or by telephone using a questionnaire. The family members of potential DCD donors were recruited from the First Affiliated Hospital, medical college of Xi'an Jiaotong University located in a metropolitan area of northwest China. The 12-item attitude questionnaire was specifically developed from the literature review with coordinator, physician, and donor's family feedback. The participants were asked to rate the queries on a 5-point Likert intensity scale.

RESULTSThe 174 participants included 56 (32.2%) women and 118 (67.8%) men. Most people were aged between 41 and 50 years (n = 63, 36.2%), 31 and 40 years (n = 59, 33.9%), and less than 30 years (n = 36, 20.7%). The top five attitudes of participants were the best person to suggest organ donation to a family was ranked as the DCD coordinator of Red Cross Organization (RCO, n = 160, 92%), donor is a hero (n = 143, 82.2%), honor to be a donor's family member (n = 136, 78.2%), improved relationship with colleagues (n = 124, 71.3%), and with recipient after donation (n = 123, 70.7%). The best person to suggest organ donation to a family was ranked as the coordinator of RCO (n = 160, 92%), doctor unrelated to transplantation (n = 104, 59.8%), social worker (n = 36, 20.7%), and doctor related to transplantation (n = 25, 14.4%). The top two reasons for non-consent to donation were that the family insisted on intact body after patient death and did not want to have surgery again (n = 51, 41.5%), and feared that they would be misunderstood by neighbors, relatives, and friends about donation (n = 28, 22.8%).

CONCLUSIONSThis study revealed initial attitudes toward DCD in China. Some data afford insight into the decision-making procedure. The concerns of potential DCD donors and their families may help professionals provide better interventions in the future.

Adult ; Attitude ; China ; Death ; Decision Making ; Female ; Humans ; Male ; Middle Aged ; Surveys and Questionnaires ; Tissue and Organ Procurement ; statistics & numerical data

OBJECTIVE To investigate the effects of the compo nents of traditional Chinese medicine （TCM）（total ginsenoside，total ketone ester of Ginkgo biloba ，total glucoside of Crocus sativus ）for invigorating qi and activating blood circulation on cognitive function of mice with chronic cerebral ischemia （CCI）and its related mechanisms . METHODS CCI model was established by stimulating bilateral common carotid arteries of C 57BL/6J mice with temperature controlled current . Model mice were randomly divided into model group ，aspirin group （positive control 10 mg/kg）and TCM group （the components of TCM for invigorating qi and activating blood circulation 33 mg/kg），sham operation group was set up additionally ，with 16 mice in each group. Except that the mice in model group and sham operation group were given water intragastrically ，the mice in the other groups were given corresponding medicine liquid intragastrically，once a day ，for 8 weeks. The latency of step down and the number of step -down errors of mice in each group were detected ；the ultrastructure of neurovascular units（NVU）in cerebral cortex and hippocampus was observed ；the protein and mRNA expressions of vascular endothelial growth factor（VEGF）and α7 nicotinic acetylcholine receptors （α7nAChRs） in cerebral tissue were detected ；the contents of VEGF，angiopoietin 1 （Ang1） and basic fibroblast growth factor（bFGF）were detected . RESULTS Compared with model group ，the latency of step -down was significantly prolonged （P＜ 0.05），and the number of step -down errors was significantly reduced （P＜0.05）in TCM group . The ultrastructural abnormalities of NVU in cerebral cortex and hippocampus were significantly improved ，and the protein and mRNA expressions of VEGF and α7 nAChRs and the contents of VEGF ，Ang1 and bFGF in cerebral tissue were significantly increased （P＜0.05）. CONCLUSIONS The components of TCM for invigorating qi and activating blood circulation can improve CCI -induced cognitive dysfunction in mice，the mechanism of which may be associated with protecting NVU ，recovering ultrastructural abnormalities of injured NVU ， and regulating the expression of proteins closely related to NVU and memory function .