Objective To activate microglia N9 cell through the oxygen deficit, and to discuss the influence to the N9 cell by ginsenoside Rb1, laying the foundation for the basic study and the clinical medicine development. Methods Through ginsenoside Rb1 intervention, the cell morphology the proliferation ability were observed, ELISA, fluorescent probe DAF-FM DA, Griess the reagent examination, were used to measure TNF-α, the O-2 output, the NO content change, chemiluminescence, the immunofluorescence method, and plastochondria membrane potential, were carried out to detect the cytochrome C content. Results Regardless of being preventive or medical gives, ginsenoside Rb1 can decline the NO,O-2,TNF-α high expression; and reduce the plastochondria membrane potential changing, the cytochrome C redistribution. Conclusion Ginsenoside Rb1 can decline N9 cell activation to a certain extent, reduce expression of the nerve toxic factor, and to stabilize mitochondrial membrane potential and distribution of cytochrome C.

OBJECTIVETo investigate the relationship between the mean CT value and bone density value and explore a rapid and accurate image segmentation method for lumbar CT values in different age groups.

METHODSWe randomly collected thin lumbar spine CT scan data from 202 patients stratified by gender and age (at the interval of 10 years). The data of each group were imported into Mimics 14.0 software to obtain independent Mask files using the new image segmentation method. The Mask files were used to fill the empty space and calculate mean, maximum, and minimum CT values.

RESULTSIn the age groups of 21-30, 31-40, 41-50, 51-60, and 61-70 years, the male subjects had mean CT values of 424.94 ± 52.23 Hu, 405.59 ± 53.60 Hu, 361.76 ± 53.50 Hu, 344.17 ± 47.76 Hu, and 332.88 ± 58.33 Hu, respectively; the values in the female group were 439.89 ± 50.99 Hu, 448.06 ± 65.89 Hu, 421.43 ± 54.74 Hu, 369.07 ± 78.27 Hu, and 304.98 ± 55.05 Hu, respectively. The mean CT value in men aged 61-70 years was significantly lower than the values in men below 50 years (P<0.05), but comparable with that in those aged 51-60 years (P>0.05); The mean CT value was significantly lower in women aged 51-70 years groups than in those aged below 50 years (P<0.05).

CONCLUSIONSThe mean CT values are correlated with the bone density values. The new image segmentation method allows rapid and accurate acquisition of the CT values in the regions of interest. The lumbar mean CT values tend to decrease with age.

Adult ; Aged ; Aged, 80 and over ; Bone Density ; Female ; Humans ; Lumbar Vertebrae ; diagnostic imaging ; Male ; Middle Aged ; Software ; Tomography, X-Ray Computed ; Young Adult