ObjectiveTo discuss the influence of health education to the pregnant women and their relatives on patients' satisfaction degree in grass-root hospitals. Methods200 pregnant women in our hospsital were divided into the experimental group and the control group, the experimental group was given systematic health education, while the control group only received the traditional reception. Then a survey was carried out in the two groups and the satisfaction degree of maternal women and their relatives with medical workers was compared. ResultsThe satisfaction degree Of maternal women and their relatives in the experimental group was higher than that of the control group. ConclusionsHealth education in gy-necology and obstetrics department and carrying out nursing with humanity can improve satisfaction degree of maternal women and their relatives to medical staffs.

Objective To research the epithelial-mesenchymal transition (EMT) effects of cuprous oxide nanoparticles (CONPs) on melanoma.Methods Cuprous oxide nanoparticles were prepared hydrothermally.The B16 cells were cultured with cuprous oxide nanoparticlesat different concentrations (5,25,50 μg/ml).The changes of the morphology of the B16 cell were observed under the inverted microscope.The effects of CONPs on B16 cell migration ability were detected through the Wound healing assay and the Transwell assay.Then cell immunofluorescence and western blotting were used to test the EMT related molecular markers, including E-cadherin, N-cadherin, Cytokeratin, and Vimentin.Results The synthesized cuprous oxide nanoparticles distribute uniformly with a diameter of 40 nm.Our study indicated that CONPs inhibited the EMT of B16 cell.A conversion process was discovered in this study.In B16 cells, CONPs inhibited B16 cell migration, promoted the expression of E-cadherin, Cytokeratin and Desmoplakin, while the expression of N-cadherin and Vimentin was repressed in protein level.Conclusion Cuprous oxide nanoparticles can significantly restrain the invasion and metastasis of melanoma cells and inhabit the EMT of B16 cells.