Severe hand-food-mouth disease can be accompanied by neurogenic pulmonary edema,whicn can rapidly lead to death.The pathogenesis of neurogenic pulmonary edema may be related to the comprehensive influences on neural factors,humoral factors and multiple bioactive substances after the central nervous system is damaged.It is of great impotance to investingate the pathogenesis of neurogenic pulmonary edema,which facilitates timely treatment and prevention of the complications.

The medical image registration between preoperative three-dimensional (3D) scan data and intraoperative two-dimensional (2D) image is a key technology in the surgical navigation. Most previous methods need to generate 2D digitally reconstructed radiographs (DRR) images from the 3D scan volume data, then use conventional image similarity function for comparison. This procedure includes a large amount of calculation and is difficult to archive real-time processing. In this paper, with using geometric feature and image density mixed characteristics, we proposed a new similarity measure function for fast 2D-3D registration of preoperative CT and intraoperative X-ray images. This algorithm is easy to implement, and the calculation process is very short, while the resulting registration accuracy can meet the clinical use. In addition, the entire calculation process is very suitable for highly parallel numerical calculation by using the algorithm based on CUDA hardware acceleration to satisfy the requirement of real-time application in surgery.
Algorithms ; Humans ; Imaging, Three-Dimensional ; Tomography, X-Ray Computed ; X-Rays

Objective To study the condition for Pseudomonas sp. W2 culture and degradation of bisphenol A(BPA). Methods The growth curve of Pseudomonas sp. W2 and BPA degradation curve were made concerned pH, temperature, aeration, carbon sources and nitrogen sources affecting Pseudomonas sp. W2 culture and BPA degradation. Results Under the conditions of pH=7, 25-35 ℃, certain carbon sources and nitrogen sources, Pseudomonas sp. W2 grew well and showed a good efficiency of BPA degradation. Conclusion Some wide conditions can meet the requirement of Pseudomonas sp. W2 growth and BPA degradation.

With the development of science and society, increasing attention has been paid to the pollution of environmental estrogens. As one of environmental estrogens, bisphenol-A has been used in many fields, but it has many aspects of toxicity. The studies on the toxicity, environmental pollution, degradation and removal of bisphenol-A have become the research emphasis in recent years. The advances in the studies of these aspects were reviewed in this article briefly.

Objective:To investigate the demands for cardiac rehabilitation information in patients with coronary atherosclerotic heart disease (CHD) and influential factors.Methods:Information demands for cardiac rehabilitation in CHD patients were surveyed by questionnaire and the influential factors were analyzed by one-way ANOVA and multi-factor analysis of variance.Results:The score of demands for cardiac rehabilitation information in CHD patients was 3.86±0.53.Among them,the most urgent top 5 items were:drug knowledge,diagnosis and treatment,basic knowledge of the heart,emergency and safety and nutrition knowledge.The top 3-demand modes were:communication with medical workers,movies or videos to take home,and lectures.The score of demands for cardiac rehabilitation information was different in different age groups.The highest score was in the patients with age less than 60.There were different demands in different characteristic groups.Conclusion:The most urgent need and mode are drug knowledge and communication with medical workers,respectively.With the age increase,the demands for patients' cardiac rehabilitation information decrease.An individualized health education strategy should be developed according to the characteristics of CHD patients.

Objective To investigate the clinical application of pressure pulse band in placenta previa cesarean section hemorrhage.Methods 130 patients with placenta previa surgery bleeding were randomly divided into two groups.The control group received conventional treatment of oxytocin,and the observation group,on the basis of the control group,in cesarean section in placenta praevia fail to control bleeding and bleeding is excessive,may surround the cervix as possible by lower fastening pressure pulse band (i.e.both ligament below),rapid delivery of uterus in abdominal cavity,stop the bleeding.Results The average time to hemostasis and surgical time of the control group were significantly higher than those of the observation group(t =5.72,13.27,P ＜ 0.05).The average blood loss,blood transfusion were significantly higher than those of the observation group (t =14.37,10.45,P ＜ 0.05).The number of transfusion was significantly higher than that of the observation group (x2 =15.93,P ＜ 0.05).There was no death case.Conclusion In placenta previa cesarean section,the application of pressure pulse band can be quickly and effectively reduce the bleeding during operation and easy to master.

0.05),while the activities of SOD of bone tissue were lower(P

Abstract: Objective To analyze the genetic evolution and molecular characteristics of the H5N6 avian influenza virus A/Changsha/1/2022(H5N6) in a human infection case in Changsha City, providing insights for the prevention and control of H5N6 avian influenza transmission to humans. Methods The sample underwent whole-genome sequencing on a third-generation sequencing platform. Reference sequences were downloaded from the NCBI and GISAID databases for comparison. The phylogenetic tree was constructed and key amino acid mutation sites were analyzed using MEGA7 software. Results The sequence analysis results revealed that the strain studied belongs to the H5 subtype Clade 2.3.4.4b branch. The PB1 gene shared 99.69% homology with the H5N6 virus A/duck/Hunan/S40199/2021(H5N6) from Hunan Province; PB2 had 98.58% homology with A/Whooper swan/Sanmenxia/Y36/2020(H5N8); and other sequences showed high homology with the strain A/Guangdong/1/2021(H5N6) discovered in Guangzhou in 2021. These results indicate that an infection with a recombinant H5N6 subtype avian influenza virus caused the case. The amino acid composition of the cleavage site was RERRRKR↓GLF, consistent with high-pathogenicity avian influenza characteristics. The HA sequence showed no mutations at the Q226L and G228S sites, indicating the preservation of characteristics binding to avian receptors. However, mutations at the S127P, S137A, T160A, T192R, and A267T sites increased the virus's affinity for humans. A deletion found in positions 59-70 of the NA protein stalk implies enhanced viral virulence in mammals. Mutations were noted in the internal genes; S622G in PB1, K389R and V598T in PB2, N409S in PA, N30D and T215A in M1, and P42S in NS1, which may enhance the virulence of avian influenza virus in infecting mice. Conclusions The H5N6 virus strain detected in a human case in Changsha City in this study is highly pathogenic, prone to bind to avian receptors, but numerous mutations at key amino acid sites facilitate infection in humans. Continuous monitoring and research on H5N6 avian influenza virus should be strengthened

To investigate the protective effects of the n-butanol extract of Potentilla anserina L. (NP) on pituitrin-induced acute myocardial ischemic injury in mice.

Objective To observe the relationship between 5-Hydroxytryptamine(5-HT)-like immunoreactive terminals and the vestibulo-parabrachial nucleus projection neurons which may express 5-HT1A receptor in the vestibular nuclear complex(VNC). Methods Retrograded-tract tracing technique combined with double labeling of immunofluorescence histochemical was used,and the stained sections were observed under a confocal laser-scanning microscope. Results Following injection of tetramethylrhodamine(TMR) into the parabrachial nucleus, many retrogradely labeled neurons were observed bilaterally within VNC,but with an ipsilateral predominance.Immunofluorescence histochemical staining showed that many neurons expressed(5-HT1A) receptor-like immunoreactivity and a large number of 5-HT immunostained fibers or terminals were found in the medial,spinal,superior,lateral vestibular nucleus(MVe,SpVe,SuVe,LVe),X nucleus and Y nucleus.Confocal laser-scanning microscopy revealed that some TMR-labeled neurons were 5-HT_1AR immunopositive,and some of the cell bodies or dendrites of TMR/5-HT1AR double-labeled neurons were closely apposed by 5-HT-like immunoreactive terminals.Conclusion The present study suggests that 5-HT may modulate vestibular signals along the VNC-parabrachial nucleus pathway via 5-HT1A receptor.