Tumor markers lay the foundation of early diagnosis for hepatocellular carcinoma (HCC).Besides alpha-fetoprotein, glypican 3 is confirmed to be a novel tumor marker for HCC with high specificity and sensitivity, especially at immediately early stage of small carcinoma. Glypican 3 has high value of clinical application and may bring new hope for precise diagnosis of HCC.

Between 40 to 60, especially after menopause, breast cancer have a higher incidence in women population. CA-153 ( carbohydrate antigen-153 ) is a tumor marker commonly used for monitoring breast cancer in clinic. However, CA-153 appears to have no clinical value for early diagnosis of breast cancer. Gene expression profiling technology becomes hot-spot through which some breast cancer related genes have been found. Moreover, the methods used for diagnosis of breast cancer also has certain shortcomings, and it is still uncertain that breast cancer is likely to be found at the early stage or be predicted in advance.

The human sclera accounts for 95％ of the surface of the eyeball,providing ample contact area which is suitable for targeted trans-scleral ocular drug delivery.Currently there are several tans-scleral sustained-release strategies,including intra-scleral delivery,episcleral delivery,as well as tans-scleral iontophoresis.Different devices and methods have their own advantages and disadvantages,for example,intrascleral delivery is somehow invasive,and episcleral delivery device needs to be made thin to prevent erosion of conjunctiva,iontophoresis needs to be frequently repeated as of its short-term effect.With the development of bio-material engineering technology,episcleral microfilm could become an ideal drug delivery route for posterior segment ocular diseases.

There have been problems in the existing multiple physiological parameter real-time monitoring system, such as insufficient server capacity for physiological data storage and analysis so that data consistency can not be guaranteed, poor performance in real-time, and other issues caused by the growing scale of data. We therefore pro posed a new solution which was with multiple physiological parameters and could calculate clustered background data storage and processing based on cloud computing. Through our studies, a batch processing for longitudinal analysis of patients' historical data was introduced. The process included the resource virtualization of IaaS layer for cloud platform, the construction of real-time computing platform of PaaS layer, the reception and analysis of data stream of SaaS layer, and the bottleneck problem of multi-parameter data transmission, etc. The results were to achieve in real-time physiological information transmission, storage and analysis of a large amount of data. The simulation test results showed that the remote multiple physiological parameter monitoring system based on cloud platform had obvious advantages in processing time and load balancing over the traditional server model. This architecture solved the problems including long turnaround time, poor performance of real-time analysis, lack of extensibility and other issues, which exist in the traditional remote medical services. Technical support was provided in order to facilitate a "wearable wireless sensor plus mobile wireless transmission plus cloud computing service" mode moving towards home health monitoring for multiple physiological parameter wireless monitoring.
Computer Systems ; Computers ; Humans ; Information Storage and Retrieval ; methods ; Internet ; Monitoring, Physiologic ; methods ; Software

Objective To investigate the lonely behavior characteristics and related factors of chronic obstruetive pulmonary disease(COPD)patients.Methods 99 eases with stable patients with COPD and 90 healthy subjects were surveyed by SDS,ESLI.Relevant factors were analyzed.Results Patients with COPD showed difierent degrees of emotional isolation[(9.90±3.97),(6.15±5.22),F=82.539,P＜0.01)]and social intercourse isolation[(8.00±3.53),(4.31±2.46),F=73.540,P＜0.01)].Emotional isolation was mainly.Moderate emotional isolation and general social intercourse isolation were predominant,and emotional(r=0.399,t=3.476,P＜0.01)and social intercourse isolation(r=0.372,t=3.154,P＜0.01)were related to depression.Conclusion Patients with COPD presented obvious emotion and social intercourse isolation behavior.

Objective:To study the antitumor effect of combination therapy with adenoviral transfer of mouse endostatin gene and cyclophosphamide chemotherapy on mouse Lewis lung cancer.Methods:Mouse endostatin gene was cloned into repli-cation-defective adenovirus by virus recombination technology to construct Ad-mES,adenovirus vector coding mouse endo-statin gene,and then its biological activities were surveyed in vivo.Animal models of mouse Lewis lung carcinoma in C57BL/6mice were established and randomly divided into4groups(n=10):including control,chemotherapy,gene therapy,and chemotherapy+gene therapy group.Mice received cyclophosphamide150mg/kg s.c.3times a day in a21-d cycle for chemotherapy;control group received0.9%normal saline100?l.Ad-mES(6?10 9 pfu/100?l)were administrated by multi-spots intra-tumor injection to every mouse in gene therapy group and combination therapy group.The tumor sizes were mea-sured at different times points.The expression of endostatin after transfer was detected by Western blot and the microvessel density(MVD)of tumor was measured by immunohistochemical examination.Results:Higher titers of recombinant aden-oviruses(Ad-mES)were constructed.Combination therapy with Ad-mES and cyclophosphamide inhibited tumor growth(P

Valproate (VPA) has long been used for the treatment of bipolar mood disorder. VPA is effective in control of mania and depression. Recent studies have demonstrated that VPA has profound neuroprotective effects in against various apoptotic stimuli. Moreover, VPA can promote neurogenesis, neuronal proliferation and differentiation. Although intensive research has been dedicated to VPA′s neuroprotection, the molecular mechanisms by which VPA protects neurons are still not fully understood. In this paper, recent progresses in the study of VPA′s neuroprotection and underlying mechanism are reviewed.

ObjectiveTo study the proper braking and bed rest time for patients with TACE operation. Methods Divided 236 patients with TACE operation into the control group (100 cases) and the experimental group (136 cases) randomly. In the control group, the braking time and the bed rest time was 8 hours and 24 hours respectively, while in the experimental group, they were 5 hours and 12 hours respectively.Compared incidence rate of complication between the 2 groups. Results The differences of complications which relevant to puncture between the two groups were not significant,P值=0.419, while the complications which irrelevant to the puncture between the two groups were significant,P

Objective To observe the effect of retrograde sinus coronary perfusion on the function of myocardial cell membrane following retrograde sinus coronary perfusion Methods Thirty two isolated rabbit hearts were randomized into 4 groups: Group A, hearts were kept beating by retrograde perfusion with normothermic oxygenated blood; Group B, hearts received retrograde coronary sinus perfusion with warm blood cardioplegia; Group C, hearts received retrograde coronary sinus perfusion with cold blood cardioplegia; Group D, hearts received retrograde coronary sinus perfusion with cold crystalloid cardioplegia After 60 min retrograde perfusion, the hearts were switched on Langendorff Neely antegrade perfused working heart model for other 60 min Free calcium in myocardial cell, myocardial membrane MDA, ATPase and myocardial cell membrane fluidity were measured Results After retrograde coronary sinus perfusion, activity of ATPase and fluidity of the myocardial cell membrane in group A were higher than those in groups B, C and D The contents of myocardial cell membrane MDA and calcium in myocardial cell following retrograde perfusion were much lower in group A than those in groups B and C There was no remarkable difference between group C and group D Conclusion Retrograde perfusion of beating heart is superior to those perfused with warm blood cardioplegia in reducing free radical production, ameliorating paradoxical calcium accumulation in myocardial cell and maintaining activity of ATPase and fluidity of the myocardial cell membrane Warm blood cardioplegia is superior to cold blood cardioplegia

The employment of college graduates becomes rigorous;it is of great importance to establish an entire process of employment instruction system.Therefore,the position of vocational advisor should be improved,and the different classification instruction should be given to different grades of college graduates,to work further with the directive of social division of labors.