AIM　To study the chemical constituents of Peucedanum decursivum. METHODS Various column chromatographies with silica gel and HPLC were employed for the isolation and purification. The structures of the compounds were elucidated on basis of spectral analyses and chemical methods. RESULTS Seven compounds were isolated from the roots of Peucedanum decursivum (Miq.) Maxim. 1 is 3′(S)-hydroxy-4′(R)-angeloyloxy-3′,4′-dihydroxanthyletin, named Decursitin D; 2 is 3′(S)-acetoxy-4′(R)-hydroxy-3′,4′-dihydroxanthyletin, named decursitin F; the others are 3′(S)-acetoxy-4′(R)-angeloyloxy-3′,4′-dihydroxanthyletin (3), Pd-C-IV (4), Pd-C-II (5), (+)-3′S-decursinol (6) and (+)-trans-decursidinol (7). CONCLUSION　Compounds 1 and 2 are new compounds, while compounds 6 and 7 were isolated from Peucedanum decursivum (Miq.) Maxim for the first time.

Chemical constituents of the aerial parts of Qianhu were compared with those present in the roots by RP-HPLC. It was found that the constituents in aerial parts of Peucedanum praeruptorum are similar and higher in content than that in the roots. So it is possible that the aerial parts can be used instead of the roots of the plant. But the chemical constituents in the aerial parts and the roots of P. decursivum are quite different, rendering it impossible to use the aerial parts instead of the roots of the plant.

Object In order to control the quality of crude drug, the total flavonoids of Epimedium koreanum Nakai were investigated Methods By repeated chromatographic methods 13 compounds were isolated and purified, and their structures were elucidated by physicochemical properties and spectroscopic methods (UV, IR, 1HNMR, 13 CNMR, HMBC, EI MS) Results They were identified as icariside II (Ⅰ), icariside I (Ⅱ), epimedoside C (Ⅲ), epimedin B (Ⅳ), icariin (Ⅴ), hyperoside (Ⅵ), hexandraside E (Ⅶ), anhydroicaritin (Ⅷ), maltol (Ⅸ), demethylanhydroicaritin (Ⅹ), insistol (Ⅺ), epimedoicarisoside A ( ⅩⅡ ) and (+) cycloolivil ( ⅩⅢ ) Conclusion Compounds Ⅶ, Ⅺ, ⅩⅢ were isolated from E koreanum for the first time, and Ⅶ was obtained from the plants of Epimedium L for the first time

Objective To investigate chemical constituents in the leaves of Euphorbia soongarica(Xinjiang origin).Methods The leaves were extracted with 95% EtOH and partitioned with petroleum ether,CHCl3,and n-BuOH,respectively.Compounds were isolated by using repeated silical gel column chromatography and Sephadex LH-20 column chromatography.Results Eight compounds were identified on the basis of physiochemical properties and spectral data as chrysoeriol(Ⅰ),phthalic acid,bis-(2,5-dimethylhexyl) ester(Ⅱ),fraxetin(Ⅲ),3-(4-O-?-D-glucopyranosyloxy-3,5-dimethoxy)-phenyl-2E-propenol(Ⅳ),fraxin(Ⅴ),3-methylellagic acid-3′-O-?-D-xylopyranoside(Ⅵ),3,3′-dimethylellagic acid-4-O-sulphate(Ⅶ),and(+)-isolariciresinol-9-O-?-D-xylopyranoside(Ⅷ).Conclusion Compound Ⅶ is a new natural product,the other seven compounds are obtained from this plant for the first time.

Alanine aminotransferase (ALT)abnormality is an indicator of the degree of liver inflammation caused by immune activation in patients with chronic hepatitis B (CHB).However,approximately half of the CHB patients with normal or mildly elevated ALT levels have concealed significant changes in hepatic histology.CHB patients with normal or mildly elevated ALT levels may have significant histopatho-logical changes in hepatic tissues,and those changes vary between HBeAg(+)and HBeAg(-)CHB patients.Attention and investigation on the clinical management of CHB patients with normal or mildly elevated ALT levels may have great significance in grasping the right treat-ment opportunity and reducing the risk of liver cirrhosis.

Objective To study the chemical constituents from the roots of Gypsophila pacifica. Methods The chemical constituents were isolated by various column chromatographic methods and their structures were identified by spectral data together with physicochemical analysis. Results Five compounds were isolated and identified as 3-O-β-D-galactopyranosyl-(1→2)-[β-D-xylopyranosyl-(1→3)]-β-D-glucuronopyranosyl gypsogenin 28-O-β-D-xylopyranosyl-(1→4)-α-L-rhamnopyranosyl-(1→2)-β-D-fucopyranoside (1), 3-O-β-D-galactopyranosyl-(1→2)-[β-D-xylopyranosyl-(1→3)]-β-D-glucuronopyranosyl quillaic acid 28-O-β-D-xylopyranosyl-(1→4)-α-L-rhamnopyranosyl-(1→2)-β-D-fucopyranoside (2), 3-O-β-D-galactopyranosyl-( 1→ 2 )-[β-D-xylopyranosyl-( 1→ 3 ) ]-β- D-glucuronopyranosyl gypsogenin 28-O-β-D-glucopyranosyl-( 1→ 3 )-[β-D-xylopyranosyl-( 1→4 ) ]-α-L-rhamnop yranosyl-( 1 → 2 )-β-D-fucopyranoside (3),3-O-β-D-galactopyranosyl-(1→2)-[β-D-xylopyranosyl-(1→3)]-β-D-glucuronopyranosyl quillaic acid 28-O-β-D-glucopyranosyl-( 1→ 3 )-[β-D-xylopyranosyl-( 1→4 ) ]-α-L-rhamnopyranosyl-( 1→2 )-β-D-fucopyranoside ( 4), 3-O-β-D-galactopyranosyl-(1→2)-[β-D-xylopyranosyl-(1→3)]-β-D-glucuronopyranosyl quillaic acid 28-O-α-L-arabinopyranosyl-(1→4)-α-L-arabinopyranosyl-(1→3)-β-D-xylopyranosyl-(1→4)-α-L-rhamnopyranosyl-(1→2)-β-D-fucopyranoside (5). Conclusion The five compounds are isolated from this plant for the first time.

Objective To observe the effects of total flavones from Herba Epimedii(TFHE)on the experimental myocardial ischemia and hemorheology of animals.Methods The model of acute myocardial ischemia of rats was established with pituitrin and the model of acute blood stasis was established with high-molecular dextran.The effects of TFHE on the electrocardiogram J-point of acute myocardial ischemia model and the hemorheology of acute blood stasis model were observed.Besides these,the effects of TFHE on coaglutaion time in mice were observed.Results As compared with the model groups,high-,middle-,and low-dosage groups(24,12,and 6 mg/kg)of TFHE could obviously improve the abnormal electrocardiogram J-point of acute myocardial ischemia model,also could effectively prevent the ascending of whole blood viscosity(high-,middle-,and low-shear rate),packed erythrocyte volume,and fibrinogen.High-and low-dosage groups(34 and 17 mg/kg)of TFHE could obviously lengthen the coaglutaion time in mice.Conclusion The results suggest that TFHE possesses protective effects on ischemic myocardium,which may better hemorheology,decrease the whole blood viscosity,prevent blood from coagulating and improve the circulatation of coronary artery.