Objective To establish blood screening and genetic detection of thalassemia trait in pregnant couples in Chang‐shou area so as to provide guidance for aristogenesis and prenatal diagnosis .Methods A total of 1 760 pregnancy in maternal and child health hospital treated from January 2013 to October 2014 were selected for study .The component of hemoglobin was ana‐lyzed as primary screening and genotype of pregnant couples were ensured in which primary screening result is positive .Results There were 27 cases suspected as α‐thalassemia (positive rate was 1 .53% ) and 25 cases suspected as β‐thalassemia(positive rate was 1 .42% ) in the primary screening(n=1 760) .The positive rate of gene carrier were 31 .51% (n=438) in women and 33 .33%(n=27) in men .Conclusion The routine screening of thalassemia could guide aristogenesis in high incidence area and provide terms of prenatal diagnosis and genetic counseling .

OBJECTIVETo explore permeability of artificial blood-brain barrier (aBBB) by oxygen-glucose deprivation combined (OGD)-induced using tetramethylpyrazine combined with puerarin in vitro.

METHODRats were divided into normal control group, model group, tetramethylpyrazine group, puerarin group, tetramethylpyrazine-puerarin group and nimodipine group. Culture rat brain microvascular endothelial cells and astrocytes in vitro and build the OGD-induced aBBB damage model. Evaluate aBBB damage characteristics by TEER, gamma-GT, AKP and LDH. Determine contents of tetramethylpyrazine, puerarin, nimodipine and calculate drug permeating concentration of OGD-induced aBBB model by HPLC.

RESULTCompared with the model, the level of TEER was lower than the control group with significant difference (P < 0.01). The levels of gamma-GT, AKP in tetramethylpyrazine group, tetramethylpyrazine-puerarin group and nimodipine group were higher than the model group, the differences were significant (P < 0.01). Compared with tetramethylpyrazine group or puerarin group, the level of AKP of tetramethylpyrazine-puerarin group increased significantly (P < 0.01). The differences of levels of TEER, gamma-GT, AKP and LDH between tetramethylpyrazine-puerarin group and nimodipinthe group were significant (P < 0.05). Tetramethylpyrazine-puerarin group has a synergistic effect of increasing TEER, gamma-GT, AKP and reducing LDH. The permeating rate in tetramethylpyrazine-puerarin group was higher than tetramethylpyrazine group and puerarin group.

CONCLUSIONTetramethylpyrazine-puerarin can permeate aBBB more easily and protect aBBB. The cause may relate to reducing the permeability of the OGD-induced aBBB.

Animals ; Blood-Brain Barrier ; drug effects ; Drug Combinations ; Glucose ; physiology ; Isoflavones ; administration & dosage ; pharmacology ; Male ; Oxygen ; physiology ; Permeability ; Pyrazines ; administration & dosage ; pharmacology ; Rats ; Rats, Sprague-Dawley

Objective To evaluate the curative effects of vacuum sealing drainage equipment on prevention and treatment of subcutaneous effusion after breast cancer radical correction. Methods A total of 122 cases of breast cancer patients from June 2006 to June 2012 were randomly divided into control group ( n=62) and intervention group (n=60). The patients of control group underwent the conventional drainage while the patients of intervention group received the vacuum sealing drainage. The situation of subcutaneous effusion, infection, skin flap necrosis, decannulation time as well as day flow conditions in two groups were observed and compared. Results The incidence of subcutaneous effusion, infection and skin flap necrosis in control group were 12. 9%, 6. 4%, 8. 1% respectively. In intervention group, the incidence rate of subcutaneous effusion was 1. 7%, and no infection, and skin flap necrosis had been observed (χ2 =7. 90,8. 31,9. 12;P<0. 01). The extubation time and day flow conditions in control group was(6. 8 ± 2. 3) d and (70 ± 38) ml, while the interventiongroupwere(10.5±3.1)dand(105±28)mlwithstatisticalsignificance(t =2.10,3.95;P <0. 05). Conclusions Simple vacuum sealing drainage device can not only prevent the subcutaneous effusion, infection and skin flap necrosis after breast cancer radical correction, but also can realize the early extubation, therefore it is worthy of promotion in clinical.