1.An animal experiment of construction of functional tissue-engineered bone with cell sheet technology.
Tao CHEN ; Yanhui WANG ; Lingxue BU ; Ningyi LI
West China Journal of Stomatology 2011;29(4):442-445
OBJECTIVETo construct functional tissue-engineered bone with cell sheet technology and method of traditional bone tissue engineering.
METHODSCanine bone marrow mesenchymal stem cells (BMSCs) were isolated with the method of density gradient centrifugation and cultured. BMSCs were induced to differentiate into osteoblasts and cultured in temperature-responsive culture dishes at 37 degrees C, 5% CO2 and saturated humidity. BMSCs cell sheet was prepared when temperature was changed to 20 degrees C. Demineralized bone matrix (DBM) and platelet-rich plasma (PRP) were prepared, and complex of DBM/PRP/BMSCs cell sheet/BMSCs was construsted and implanted under the left latissimus dorsi muscle. Complex of DBM/PRP/BMSCs was implanted under the right latissimus dorsi muscle.
RESULTSWhen temperature dropped at 20 degrees C, BMSCs detached automatically from the temperature-responsive culture dishes and formed an intact cell sheet. The osteogenesis of the DBM/PRP/BMSCs cell sheet/BMSCs group was better than that of the DBM/PRP/ BMSCs group.
CONCLUSIONCell sheet technology combined with traditional bone tissue provides a new way for construction of ideal functional tissue-engineered bone.
Animal Experimentation ; Animals ; Bone Marrow Cells ; Bone and Bones ; Cells, Cultured ; Mesenchymal Stromal Cells ; Osteoblasts ; Osteogenesis ; Platelet-Rich Plasma ; Stromal Cells ; Tissue Engineering
2.Repurposing antimycotic ciclopirox olamine as a promising anti-ischemic stroke agent.
Hongxuan FENG ; Linghao HU ; Hongwen ZHU ; Lingxue TAO ; Lei WU ; Qinyuan ZHAO ; Yemi GAO ; Qi GONG ; Fei MAO ; Xiaokang LI ; Hu ZHOU ; Jian LI ; Haiyan ZHANG
Acta Pharmaceutica Sinica B 2020;10(3):434-446
Ischemic stroke is a severe disorder resulting from acute cerebral thrombosis. Here we demonstrated that post-ischemic treatment with ciclopirox olamine (CPX), a potent antifungal clinical drug, alleviated brain infarction, neurological deficits and brain edema in a classic rat model of ischemic stroke. Single dose post-ischemic administration of CPX provided a long-lasting neuroprotective effect, which can be further enhanced by multiple doses administration of CPX. CPX also effectively reversed ischemia-induced neuronal loss, glial activation as well as blood-brain barrier (BBB) damage. Employing quantitative phosphoproteomic analysis, 130 phosphosites in 122 proteins were identified to be significantly regulated by CPX treatment in oxygen glucose deprivation (OGD)-exposed SH-SY5Y cells, which revealed that phosphokinases and cell cycle-related phosphoproteins were largely influenced. Subsequently, we demonstrated that CPX markedly enhanced the AKT (protein kinase B, PKB/AKT) and GSK3 (glycogen synthase kinase 3) phosphorylation in OGD-exposed SH-SY5Y cells, and regulated the cell cycle progression and nitric oxide (NO) release in lipopolysaccharide (LPS)-induced BV-2 cells, which may contribute to its ameliorative effects against ischemia-associated neuronal death and microglial inflammation. Our study suggests that CPX could be a promising compound to reduce multiple ischemic injuries; however, further studies will be needed to clarify the molecular mechanisms involved.