Objective To probe into the result of treatment for lower limb occlusing diseases by percutaneous endovascular rotating cut with sucking thrombus combined with stent placement. Methods The skill of rotating cut with sucking the thrombus percutaneously was initially used, and if the artery was found to be narrowed after the procedure stent placement was then be undertaken. Results By using this method, the patients right limb arteries including the superficial famoral artery the popliteal artery and the more distal branches were recanalized persisting normally during six mouths follow-up. Conclusion Percutaneous endovascular rotating cut with sucking thrombus combined with stent placement is safe, effective and reliable for treating the occlusive diseases of the lower extremities.

Objective To study the effects of embryonic neural stem cells transplantion on trauma of red nucleus neu-rons of the rats with spinal cord injury.Methods NSCs in logarithmic phage were labeled with BrdU,a Sprague Dawley rat mode of spinal cord injury (SCI) was developed with electrocircuit control spinal cord injuring device.Thirty SD rats were randomly divided into three groups: sham group,SCI group and NSC group.The NSCs were trans-planted into injured site three days after SCI.Then NSCs labeled with Brdu were detected by immunohistochemisty,rubrospinal tract (RST) neurons were labeled by retrograde transport of the horseradish peroxidase (HRP) from the lesion site,which were taken by damaged axons and remained in the neurons,then the labeled red nucleus (RN) neurons were counted.Hind limb function of experimental rats was evaluated by a blinder observer using BBB open field locomotion rating score.Results BrdU positive NSCs were detected in the spinal cord after transplantation,the number of RST neurons labeled by HRP in NSC group was more than that in SCI group (P <0.01),the BBB score of NSC group was higher than SCI group (P <0.01).Conclusion The transplanted NSCs can survive in the injured site of spinal cord and protect RN,then promote more remarkably functional recovery after SCI.

Objective To compare of intervention embolization and operation in the treatment of brain aneurysms.Methods 60 cerebral aneurysm patients using random number table method were divided into two groups each 30 cases,group A given intervention embolization;group B given surgical operation treatment.They were followed for 3months,the bleed Glasgow results score,matrix metalloproteinases 2(MMP-2) level change and the length of time were recorded.Results After treatment,A group total effective rate was 73.4％,postoperative treatment to bleed again rate was 10.0％ (3/30),B group was 76.7％,6.7％ (2/30),two groups had no statistically significant differences (x2 =0.09,0.22,all P ＞ 0.05 ) before treatment M MP-2 level between ;The two groups was statistically significant ( t =1.21,P ＞ 0.05 ) ;Two groups after surgical treatment 3 d MMP-2 levels were ( 20.1 ± 8.7 ) μg/L,( 35.7 ±8.9 ) μg/L respectively,which was statistically significant ( t =3.33,P ＜ 0.05 ).A group of hospitalization time (8.6 ±1.5 ) d,group B ( 13.2 ± 1.3 ) d,the difference between two groups was statistically significant ( t =3.18,P ＜ 0.05 ).Conclusion For brain aneurysm patients,the intervention embolization had no obvious difference in curative effect with operation,but could reduce the time in hospital and significantly reduced MMP-2 level.

Objective To explore the clinical characteristics of patients with Moyamoya disease in the southwest of Shandong province and evaluate the efficacy of cerebral revascularization treatment in it.Methods Clinical data of 96 patients with Moyamoya disease,admitted to our hospital form March 2011 to December 2013,were analyzed retrospectively and compared with those in Japan,South Korea and Inland.Results The clinical characteristics of patients with Moyamoya disease in the southwest of Shandong province were similar with that in Japan,South Korea and Inland,but enjoyed their own particularity:the age distribution showed two peaks (aged 0-10 and aged 31-50) and the sex ratio (female＞male) was similar to those among Japanese and Korean patients,but the age distribution was more concentrated;the incidence of children (12.5％) was significantly lower than that of Japan and South Korea;the main type of Moyamoya disease was the ischemic one (80.2％);the occurrence of transient ischemic attack was higher than that in Japan,South Korea and Inland;the incidences of epilepsy and cerebral infarction were lower than those in Japan,South Korea and Inland;the incidence of cerebral hemorrhage (19.8％) was similar to Japan and Inland,and significantly lower than that in Korea.The clinical symptoms of most Moyamoya disease patients were improved with the treatment of extracranial-intracranial revascularization;and the treatment outcomes showed no significant difference between Chinese,Japanese and Korean.Conclusions The clinical features and pathogenesis mechanism of Moyamoya disease in the southwest of Shandong province may be different form Japan and South Korea.Extracranial-intracranial revascularization is a effective treatment for Moyamoya disease.

Hippophae rhamnoides is a traditional Chinese medicine with homology of medicine and food， which has the effects of relieving cough and resolving phlegm， strengthening the stomach and digestion， and promoting blood circulation and resolving blood stasis. H. rhamnoides contains not only flavonoids， phenols， proteins， vitamins， and amino acids but also abundant polysaccharides. In order to explore the functional value and current research status of H. rhamnoides polysaccharides， this study systematically summarized the extraction process， structural characteristics， pharmacological effects， and mechanism of action of H. rhamnoides polysaccharides by reviewing Chinese and foreign literature. The results showed that H. rhamnoides polysaccharides have anti-tumor， hypoglycemic， antioxidant， immune regulation， anti-inflammatory， and anti-hyperlipidemia functions and could improve intestinal microbiota. There were various extraction processes for polysaccharides， including hot water extraction， microwave extraction， ultrasonic extraction， enzymatic extraction， flash extraction， ultrasonic-microwave synergistic extraction， emerald hot water extraction， enzymatic-ultrasonic synergistic extraction， etc. Based on comprehensive analysis， hot water extraction is suitable for industrial development and application. However， multiple homogeneous polysaccharides have been isolated and purified from H. rhamnoides polysaccharides， but their efficacy， structure， and structure-activity relationship still need to be further explored and studied. This study can provide a reference for the research and development of H. rhamnoides polysaccharides.