The development of female germ cell is the cornerstone for animal reproduction. Mammalian oocyte and early embryo have many distinct phenomena and mechanisms during their growth and development, involving series dynamic changes of protein synthesis/degradation and phosphorylation. Research on the regulatory mechanism of oocyte division, maturation, and developmental principle of pre-implantation embryo is an important topic in the field of animal developmental biology. Proteomics using all of proteins expressed by a cell or tissue as research object, systematically identify, quantify and study the function of all these proteins. With the rapid development of protein separation and identification technology, proteomics provide some new methods and the research contents on fields of oogenesis, differentiation, maturation and quality control, such as protein quantification, modification, location and interaction important information which other omics technology can not provide. These information will contribute to uncover the molecular mechanisms of mammalian oocyte maturation and embryonic development. And it is great significant for improving the culture system of oocyte in vitro maturation, the efficiency of embryo production in vitro, somatic cell clone and transgenic animal production.
Animals ; Cells, Cultured ; Embryonic Development ; Female ; Humans ; Mammals ; growth & development ; Oocytes ; metabolism ; Oogenesis ; Pregnancy ; Proteomics

Objective To compare of intervention embolization and operation in the treatment of brain aneurysms.Methods 60 cerebral aneurysm patients using random number table method were divided into two groups each 30 cases,group A given intervention embolization;group B given surgical operation treatment.They were followed for 3months,the bleed Glasgow results score,matrix metalloproteinases 2(MMP-2) level change and the length of time were recorded.Results After treatment,A group total effective rate was 73.4％,postoperative treatment to bleed again rate was 10.0％ (3/30),B group was 76.7％,6.7％ (2/30),two groups had no statistically significant differences (x2 =0.09,0.22,all P ＞ 0.05 ) before treatment M MP-2 level between ;The two groups was statistically significant ( t =1.21,P ＞ 0.05 ) ;Two groups after surgical treatment 3 d MMP-2 levels were ( 20.1 ± 8.7 ) μg/L,( 35.7 ±8.9 ) μg/L respectively,which was statistically significant ( t =3.33,P ＜ 0.05 ).A group of hospitalization time (8.6 ±1.5 ) d,group B ( 13.2 ± 1.3 ) d,the difference between two groups was statistically significant ( t =3.18,P ＜ 0.05 ).Conclusion For brain aneurysm patients,the intervention embolization had no obvious difference in curative effect with operation,but could reduce the time in hospital and significantly reduced MMP-2 level.

Objective:To discuss the surgical efficacy of neuroendoscopic transsphenoidal approach for the removal of pituitary cystic lesion.Methods:Clinical data and efficacy of 32 patients with neuroendoscopic transsphenoidal surgery and pathological diagnosis of pituitary cystic lesion in the Affiliated Hospital of Jining Medical University from March 2013 to May 2019 were retrospectively analyzed.Results:Of the 32 patients, 29 patients were pathologically diagnosed with Rathke cysts and 3 patients with pituitary arachnoid cysts. The content of cyst could be completely removed and the relationship between cyst and sellarseptum and subarachnoid space could be clearly observed by using endoscopy. After followed-up for 0.5-1.0 year, headic, dizziness and visual impairment were improved. One patient relapsed, without serious complications or death.Conclusions:Transsphenoidal neuroendoscopic surgery is a safe and effective treatment for pituitary cystic lesion.

Traumatic spinal cord injury (SCI) has devastating effects on patients′ physical and mental health. Autophagy is widely involved in various physiological and pathological processes of the body and plays a key role in spinal cord injury. The mammalian target of rapamycin (mTOR) is the main regulator of autophagy. mTOR regulation of autophagy is closely related to the pathological process of spinal cord injury, and can effectively promote the recovery of spinal cord injury. Therefore, mTOR is a promising target for treatment of spinal cord injury. This article reviews the role of mTOR signal transduction pathway in the regulation of autophagy in spinal cord injury, in order to provide reference for follow-up research.

Due to the features of strong heterogeneity,difficult early diagnosis,poor prognosis,and high fatality rate,hepatocellular carcinoma (HCC) has become an important disease which threatens the health of the Chinese population.Accurate early diagnosis is crucial to improving the success rate of liver cancer resection and reducing postoperative recurrence and metastasis,and its core is the screening and validation of biomarkers for early diagnosis.Isobaric tags for relative and absolute quantitation (iTRAQ) and stable isotope labeling with amino acids in cell culture are important parts of proteomics technology,and iTRAQ has become the most important technique in quantitative proteomics technology due to its advantages of high throughput,high quantitative accuracy,and no limitation by sample source.This article reviews the research advances in molecular mechanism of the development and progression of HCC and screening of markers,in order to establish a theoretical foundation for in-depth understanding of the molecular mechanisms of the development and progression of HCC and the development of new biomarkers.