Objective To analyze the characteristics of the operating room occupational exposure and its reason of the occurrence; to formulate the occupational exposure prevention and control interventions of operating room and have a evaluation the interventions.Methods Based on data from occupational exposure report of the hospital medical staff occupational exposure monitoring system from January 1,2009 to December 31, 2015, occupational exposure was summarized that happened six years in Operating Room, and analyze the characteristics of the operating room occupational exposure, as well as the causes, factors of occupational exposure, the effect of prevention and control interventions of occupational exposure.Results It was a comparison between occupational exposures occurred in the operating room and the total occupational exposure in the hospital that gender has differences(χ2=26.90,P<0.01). Males constitute ratio in the operating room is higher than that of hospital, but exposure type comparison showed that there was no significant difference between the operating room and the hospital(χ2=0.42,P>0.05). The mainly occupational exposure was sharps' injuries. Comparison showed that doctors proportions was significantly higher in operating room than hospital (χ2=46.81,P<0.01). In the operating room, the ratio of occupational exposure in nurses was higher than in doctors, and the same trend presented in the hospital as well. After interventions, the ratio decreased from the occupational exposure during 2009 and 2011 (before intervention) compared with the occupational exposure happening in 2012 to 2014 in operating room (after intervention) (χ2=11.02,P<0.01). After intervention, the exposure constituent rate in the time of 2012 to 2014 decreased as well comparing the rate during 2009 and 2011 (χ2=11.68,P<0.01).Conclusions Operating room is one of the main places of occupational exposure. In operating room, mainly occupational exposure was sharps' injuries and blood, body fluid exposure. The proportion of doctors is higher than nurses who occurs occupational exposure. To formulate intervention measures against operating room occupational exposure features, and supervise in real-time can reduce incidence of surgery-related personnel occupational exposure.