Objective To investigate the prevalence and influencing factors in senile cataract in Huzhou city.Methods 1 680 cases of healthy physical examination elderly population were selected as the research subjects in Huzhou city.To investigate the prevalence of cataract in the elderly population.The correlation of the prevalence of senile cataract,visual impairment and sex,age was studied.The correlation of the prevalence of senile cataract and diabetes,hypertension,smoking was analyzed.At the same time,Logistic regression analysis was used to analyze the influence factors of senile cataract.Results In 1 680 cases of elderly people,299 cases of cataract,the prevalence rate of cataract was 17.79%.With age increased,the prevalence of senile cataract was gradually increased.The preva-lence of cataract in female was 21.80%,which was higher than 15.82% in male,and the difference was statistically significant (χ2 =9.083,P <0.01).The visual acuity of senile cataract patients would increase with age.Moreover, the visual acuity of female patients was higher than that of male patients.The prevalence of cataract in diabetic group was 64.58%,which was higher than 17.89% in non -diabetes group,the difference was statistically significant (χ2 =65.457,P <0.01 ).The prevalence of cataract in hypertension group was 61.48%,which was higher than 14.31% in non -hypertension group,and the difference was statistically significant (χ2 =163.538,P <0.01).The prevalence of cataract in smoking group was 80.39%,which was higher than 16.94% in non - smoking group,the difference was statistically significant (χ2 =130.046,P <0.01).Logistic regression analysis showed that gender,age, diabetes,hypertension,smoking,and so on were the factors affecting the incidence of cataract in the elderly popula-tion.Conclusion The prevalence of cataract in the elderly population in Huzhou city need to pay attention to the local health department.

Objective:Based on the principle that the aggregation-induced emission (AIE) fluorescent probe 6PD-DPAN could bind and aggregate with bacteria, and the fluorescence intensity could reflect the quantity of bacteria, a new method for rapid, convenient, and accurate bacterial drug sensitivity testing was established, which provided a basis for rapid and accurate clinical drug use.Methods:This was a methodological evaluation study. A total of 107 clinical isolates were collected from Houjie Hospital of Dongguan City from January to December 2022, among which 46 isolates were used for the establishment of the new method, and 61 isolates were used for methodological validation. The minimum inhibitory concentration (MIC) determined by broth microdilution method was used as the gold standard, and three antibacterial drugs, gentamicin, levofloxacin, and cefotaxime, were used as experimental drugs. The AIE plate was incubated for 4 hours, and the fluorescence intensity was measured every half an hour to draw a fluorescence change curve. The MIC results were compared with the CLSI breakpoints to determine the bacteria as sensitive, intermediate, or resistant. To simplify the detection process, the ratio of fluorescence intensity at 4 hours(R) was calculated, and the ROC curve was used to analyze the efficacy of R in determining bacterial growth and establish its cutoff value. The new method was used to determine the MIC of 61 clinical isolates, with broth microdilution method as the gold standard. The basic consistency, categorical consistency, very major errors, and major errors of the new method were analyzed, and the consistency between the two methods was determined by the Kappa test.Results:ROC curve analysis of the R after 4 hours of culture: The cut-off value was 3.0, with both sensitivity and specificity for determining bacterial growth being 100%. The median (interquartile) R for bacterial growth inhibition was 11.1 (8.6, 14.4); the median R-value for bacterial growth was 1.1 (1.0, 1.2). Compared to the gold standard, the newly established method showed 100% (61/61) essential agreement in detecting MICs of 61 clinical isolates, with a categorical agreement of 96.7% (59/61). There were no very major or major errors, and the Kappa value was 0.94, indicating good consistency between the newly established method and the microbroth dilution method.Conclusions:This study successfully established a new method for bacterial drug sensitivity testing based on AIE technology, which could obtain satisfactory results within 5 hours, providing a basis for early precision drug treatment in clinical practice.