1.Effect of ?,?-carotene in improving H_2O_2 induced damage of osteoblasts
Lingpeng PEI ; Fuhui DONG ; Bodi HUI ; Jia ZHU
Journal of Xi'an Jiaotong University(Medical Sciences) 1982;0(01):-
Objective To investigate the effect of ?,?-carotene in enhancing the function of anti-oxidative damage in osteoblasts.Methods M3T3-E1 osteoblasts were randomly divided in five groups: control group,model group,and ?,?-carotene group [low-dose(1?10-8mol/L),medium-dose(1?10-7mol/L),high-dose(1?10-6mol/L)].The activity of cells,superoxide dismutase(SOD),the content of reactive oxygen species(ROS),lipid oxygen(LPO),and membrane fluidity were tested and compared. Results Compared with those in ?,?-carotene groups,the activity of cells,SOD activity and membrane fluidity in the model group were significantly decreased(P
2.Inhibitory effects of miR-146a on retinal inflammation induced by high glucose in human retinal endothelial cells
Shun GU ; Pengfei ZHAN ; Wenjuan WANG ; Xiaolu WANG ; Tingting WEI ; Lingpeng ZHU ; Yangningzhi WANG ; Li YIN ; Tianhua XIE ; Yong YAO
Chinese Journal of Experimental Ophthalmology 2020;38(9):733-739
Objective:To observe the effects of miR-146a on human retinal endothelial cell (HREC) under high glucose condition.Methods:Total of 57 cases diagnosed as diabetic mellitus and 40 cases with diabetic retinopathy (DR) in Wuxi People's Hospital Affiliated to Nanjing Medical University from October to December 2013.Forty-one healthy volunteers were enrolled and served as control group.The clinical data and venous blood samples of subjects were collected.HRECs were cultured in normal glucose (5.5 mmol/L) or high glucose medium (30 mmol/L). Real-time PCR was used to detect the expression of miR-146a.The cultured HRECs were transfected with miR-146a mimic, mimic negative control, inhibitor and inhibitor negative control by lipofectamine2000, respectively.The expression of miR-146a and intercellular cell adhesion molecule-1 (ICAM-1) mRNA was examined by real-time PCR and the expression of nuclear factor-кB (NF-кB) p65 and NF-кB p65 Ser536 was detected by Western blot assay. Results:The relative expression of miR-146a mRNA in the diabetic mellitus group and DR group was 0.36±0.08 and 0.27±0.08, respectively, which were significantly lower than 1.00±0.16 in the control group (both at P<0.01). The expression of miR-146a mRNA was 0.37±0.11 in the high glucose group, which was lower than 1.00±0.18 in the normal control group ( t=5.57, P<0.01). The relative expression of miR-146a mRNA in the miR-146a mimic group was 2 540.00±105.00, which was significantly higher than 61.00±17.90 in the miR-146a mimic control group; The relative expression of miR-146a mRNA in the miR-146a inhibitor group was 0.04±0.01, which was significantly lower than 0.88±0.04 in the miR-146a inhibitor control group ( t=23.23, 17.12; both at P<0.01). The relative expression of ICAM-1 mRNA in the miR-146a mimic group was 0.35±0.12, which was significantly lower than 1.00±0.13 in the miR-146a mimic control group; The relative expression of ICAM-1 mRNA in the miR-146a inhibitor group was 2.74±0.48, which was significantly higher than 1.00±0.16 in the miR-146a inhibitor control group ( t=3.58, 3.37; both at P<0.05). The relative expression of NF-кB p65 Ser536 in the miR-146a mimic group was 0.43±0.03, which was significantly lower than 1.07±0.09 in the miR-146a mimic control group ( t=6.74, P<0.01). The relative expression of NF-кB p65 Ser536 in the miR-146a inhibitor group was 2.08±0.12, which was significantly higher than 1.00±0.01 in the miR-146a inhibitor control group ( t=8.76; P<0.01). Conclusions:miR-146a can reduce inflammation of HREC in high glucose condition through inhibiting ICAM-1 expression and NF-кB phosphorylation.
3.Research progress on the role of cell aging in dry age-related macular degen-eration
Mengxia CAI ; Tingting WEI ; Lingpeng ZHU ; Yong YAO
Recent Advances in Ophthalmology 2024;44(4):324-328
Dry age-related macular degeneration(AMD)is a degenerative disease affecting the macular region of the retina,and aging changes in retinal and choroidal tissues are an important factor in AMD pathogenesis.Cell aging is an irre-versible state of cell cycle arrest triggered by certain physiological processes or stressful injury,affecting a variety of physi-ological and pathological processes.An increasing number of studies have shown that cell aging plays an essential role in the occurrence and development of AMD.This paper reviews the mechanisms of cell aging and its relationship with dry AMD,aiming to provide new ideas for the treatment of dry AMD.