1.Change of lymphocyte KCa3.1 and CaN-NFAT signaling pathway in Kazak hypertensive patients from Xinjiang
Lingpeng WANG ; Lihua ZHAO ; Juan LIU ; Leiyu CAO ; Jian LUO
Chinese Journal of cardiovascular Rehabilitation Medicine 2017;26(4):355-359
Objective: To study changes of peripheral blood lymphocyte calcineurin/ nuclear factor of activated T cells (CaN/NFAT) signaling pathway and calcium activated potassium channel (KCa3.1) expression in Kazak hypertensive patients with high high sensitive C reactive protein (hsCRP) level from Xinjiang, and explore the mechanism of lymphocyte potassium channel involving in inflammation of hypertension.Methods: Kazak hypertensive patients from Xinjiang, who were first diagnosed in our heart center without antihypertensive therapy from Feb 2014 to Nov 2014, were selected.According to detected hsCRP level, a total of 50 hsCRP≥3mg/L patients were enrolled as high hsCRP group, and 50 hsCRP<3mg/L patients were enrolled as normal hsCRP group.Gene and protein expression levels of KCa3.1 and tumor necrosis factor (TNF)-α, and activities of lymphocyte CaN and NFAT were measured and compared between two groups.Results: Compared with normal hsCRP group, there were significant rise in expression levels of lymphocyte KCa3.1 mRNA [(0.86±0.16) vs.(2.48±0.22)], TNF-α mRNA [(1.02±0.15) vs.(2.90±0.13)], KCa3.1 protein [(1.00±0.02) vs.(2.46±0.04)] and TNF-α protein [(1.01±0.02) vs.(2.04±0.06)], and activities of CaN [(1.04±0.15) vs.(2.83±0.08)] and NFAT [(0.96±0.06) vs.(2.65±0.07)] in high hsCRP group, P<0.01 all.Conclusion: In Kazak hypertensive patients with inflammatory reaction from Xinjiang, KCa3.1 and CaN-NFAT signaling pathway expression rises, suggesting that lymphocyte KCa3.1 may be involved in occurrence and development of hypertension via CaN/NFAT signaling pathway.
2.Changes of contents of acid phosphatase in Oncomelania snails infected with Schistosoma japonicum
Lingpeng LIU ; Libo XIAO ; Changhao HE ; Qing FENG
Chinese Journal of Schistosomiasis Control 1991;0(05):-
Objective To observe the contents of acid phosphatase (ACP) of Oncomelania snails infected with Schistosma japonicum.Methods Both the blood lymphocytes of positive and negative Oncomelania snails were dyed with the histochemistry staining method and their ACPs were observed under a microscope. Results The contents of ACP in the positive Oncomelania snails increased, being significantly higher than those in the negative snails. Conclusion The contents of ACP increase in Oncomelania snails infected with Schistosoma japonicum and ACP may have the defence function.
3.Structural changes of a bacteriophage upon DNA packaging and maturation.
Wenyuan CHEN ; Hao XIAO ; Xurong WANG ; Shuanglin SONG ; Zhen HAN ; Xiaowu LI ; Fan YANG ; Li WANG ; Jingdong SONG ; Hongrong LIU ; Lingpeng CHENG
Protein & Cell 2020;11(5):374-379