Objectives To investigate the effects of military stress and psychological resilience on job burnout and mental health in military personnel and construct the relationship among resilience,job burnout and mental health during military stress.Methods By cluster random sampling,520 personnel in army taking part in the war-game were selected as the experimental group,and another group of 520 who not taking part in the wargame were selected as the control group.After one week of the war-game,the experimental group and control group were investigated by Military Personnel Mental Disorder Prediction Scale,Military Job Burnout Scale and Military Personnel Resilience Scale.The acquired datum were analyzed by t-test,correlation analysis,regression analysis and structural equation modeling analysis.Results (1) The total scores of mental disorder prediction and factor scores of psychosis,depression,mania,neurosis,personality disorder were((15.85±9.30),(0.36±0.91),(1.10±1.53),(2.66±1.78),(1.16± 1.57),(2.04± 1.83)) and the total scores of job burnout and its all factor scores were((23.05±9.40),(5.13±2.47),(3.19±2.18),(5.16±3.01),(1.71 ± 1.66),(3.29±2.21)) of experimental group were significantly higher than control group((11.20±6.24),(0.11 ±0.53),(0.55± 1.10),(1.89± 1.50),(0.51± 1.02),(1.10±1.13),(17.92±9.64),(4.28±2.92),(2.64±2.06),(3.84±2.46),(1.26± 1.37),(2.77±2.43),P=0.000).(2)Psychological resilience negatively correlated with job burnout and mental health(r=-0.52～-0.09,P＜0.05),and job burnout positively correlated with mental health (r=0.12 ～ 0.57,P＜0.01).(3) Under the military stress,psychological resilience was the accommodation to effect the mental health and job burnout of military personnel.(4)The analysis of structural equation modeling showed that psychological resilience had a direct effect on job burnout(the path coefficient was-0.56,P＜0.01),but it had no significant effect on mental health (the path coefficient was-0.06,P=0.302).Job burnout had a direct effect on mental health (the path coefficient was 0.73,P＜0.01),and the structural equation modeling fitted the data very well(x2/ df =3.138,GFI =0.943,AGFI =0.911,NFI =0.930,RFI =0.905,IFI =0.952,TLI =0.933,CFI =0.951,RMSEA =0.065).Conclusion Military stress can lead to job burnout and mental disorders that could be moderated by psychological resilience.Job burnout can fully mediate the relation between psychological resilience and mental health.Psychological resilience effects mental health by job burnout indirectly.

Objective:To explore personality change and its association with lncRNA expression of peripheral blood mononuclear cells in patients with epilepsy.Methods:Fifty-eight epilepsy patients recruited by the convenient sampling were assessed utilizing personality diagnostic questionnaire(PDQ) for personality change screening.The expression levels of lncRNA in peripheral blood of study group and the controls were investigated by qRT-PCR.Descriptive statistical analysis, correlation analysis, regression analysis and ROC analysis were employed for data processing.Results:There were 9 of schizoid (S≥4), 11 of schizotypal(S≥5), 17 of paranoid (S≥4) and 15 of compulsive (S≥4) personality change in epilepsy patients, and 52 patients had different types personality changes(89.66%).Schizoid, schizotypal, paranoid and compulsive personality changes were negatively correlated with expression levels of NONHSAG012869(PR3), NONHSAT006265(PR4), ENST00000581634(PR6) and ENST00000524610(PR8) ( r=-0.46--0.71, P<0.05 or 0.01).PR1, PR3, and PR8 had significant predictive effects on schizoid personality change ( P<0.01), PR4, PR8 had a significant predictive effect on schizotypal personality change ( P<0.01), PR3, PR4 and PR6 had significant predictive effects on paranoid personality change( P<0.05), and PR4, PR5, PR8 had significant predictive effects on compulsive personality change ( P<0.05).The effects of lncRNAs on the personality change variance accounted for 0.36, 0.30, 0.40, 0.20 respectively.ROC curve analysis of the diagnostic value of lncRNA expression level on personality change in the epilepsy group showed that NONHSAG012869 (PR3), NONHSAT006265(PR4), ENST00000581634(PR6) and ENST00000524610(PR8) had certain diagnostic value for personality change.The area under curve(AUC)=0.650-0.682, P<0.05, 95% CI: 0.546-0.784. Conclusion:Schizoid, schizotypal, paranoid, and compulsive personality change are common in epileptic patients, and the expression level of peripheral blood lncRNA has a certain diagnostic value for personality change.

ObjectiveTo predict the target genes of miRNA associated with hsa_circRNA_102802 and hsa_circRNA_104597 using bioinformatics methods,and analyse the biological process and signaling pathway.MethodsTargetscan,miranda and mirbase three online database were used to predict target genes of miRNAs which are complementary to circRNAs.Target genes of miRNAs prediction results were taken their intersection of three online database,then take their collection as total target genes of miRNAs.The results were analyzed by gene ontology(GO) and KEGG pathway analysis using FunNet.Results199 target genes of has_miR_204_5p,has_miR_809,has_miR_520_5p,has_miR_423_5p,has_miR_617 and has_miR_877_5p were gotten from the intersection of 3 databases;and 410 targets of has_miR_659_3p,has_miR_9_5p,has_miR_661,has_miR_576_3p,has_miR_548d_5p,has_miR_548a_5p,has_miR_548b_5p,has_miR_876_5p and has_miR_744_5p were gotten from the intersection of 3 databases.GO analysis showed that Target genes involved in diverse biological processes including central nervous system,such as cortex development,axon guidance and extension,synaptic transmission,as well as the process of learning and memory (P<0.05).Enriched pathways were also revealed target genes involved in the signal path including the axon guidance,glutamic acid synapses,Wnt signaling pathway,ErbB signaling pathway,mTOR signaling pathway and VEGF signaling pathway,which connect closeiy with depression.hsa_circRNA_102802 and hsa_circRNA_104597 may play an important role in depression.Conclusionshsa_circRNA_102802 and hsa_circRNA_104597 may be associated with the pathologenesis of depression disorder.

Objective To investigate the differential expression of microRNA (miRNA) in schizophrenia (SZ) patients,and explore the comorbidity of SZ and depression disorder based upon miRNA expression.Methods Affymetrix array analysis was used to investigate the differentially expressed miRNA in SZ patients firstly,and then quantitative real-time polymerase chain reaction (qRT-PCR) was further carried out to confirm the selected miRNA in peripheral blood mononuclear cells of 40 SZ patients,whom were administered by Positive and Negative Syndrome Scale (PANSS) and the selected miRNAs in depression disorder patients has also been confirmed by Affymetrix array analysis and qRT-PCR in our previous studies.Results Affymetrix array analysis indicated that there existed 33 miRNAs which differentially expressed (32 up-regulated and 1 down-regulated) compared with normal controls.qRT-PCR results suggested that the expression of 8 miRNAs (miR-1273d,miR-1303,miR-3064-5p,miR3131,miR-3687,miR-4428,miR-4725-3p and miR-5096) were significantly up-regulated in SZ;the miRNA differentially expressed in depression disorder patients also had differential expression in SZ patients (P＜0.05).There were significant correlation between the miRNAs differentially expressed in depression disorder patients and in SZ patients (P＜0.01).MiR-1972 differentially expressed in depression disorder patients had significant positive correlation with the positive symptoms of PANSS (P＜0.05),and miR-26b was positively correlated with composite factor (P＜0.05).Conclusion Comorbidity of SZ and depression disorder is observed not only on the clinical symptoms,but on the molecular genetic basis.

Objectives To explore the decade-feature of military personnel' s psychosomatic health and Its Influence factors.Methods By random cluster sampling,a total of 11362 military personnel ( including army,navy and air-force) were tested by Chinese Psychosomatic Health Scale (CPSHS) from 80' ( n=1100),90' ( n=8000),2000' ( n =2262)then employing SPSS statistic 17.0 program for data analysis by using ANOVA,LSD multiple-comparison,and stepwise regression analysis.Results The factor of eye-ear( 80s' ( 1.17 ± 1.68),90s'( 1.19 ± 1.64 ),2000s' ( 1.12 ± 1.48 ) ) had no significant difference in three decades (P ＞ 0.05 ) ; military personnel' s respiratory system,cardiovascular system,alimentary system,bone-muscle,skin,reproductive-endocrine system,nervous system,anxiety,depression,psychotic traits,family history,mental heath,physical health,and total psychosomatic health psychosomatic health had significant differences in the three decades (P＜ 0.05 ～0.01 ).The stepwise regression analysis revealed that decade,length of military service,urban-rural,marital state had significant effect on total psychosomatic health( decade:t =- 12.452,length of military service:t =-0.024,urban-rural:t=-0.066,marital status:t=0.119,P＜0.012 ～0.000).Conclusions The level of military personnel's psychosomatic health is improved from 80' s,90' s of last century to the year of 2000; the influenced factors of military personnels' psychosomatic health are including decade,length of military service,rural-urban,and marital status.

ObjectiveTo explore the decade-feature of military personnel' mental maladjustment and its influenced factors.MethodsBy random cluster sampling,a total of 10883 military personnel( including army,navy and air-force) were tested by military mental maladjustment scale (MMMS) in three decades (80s' 90s',2000),then employing SPSS 17.0 for data analysis by using ANOVA,LSD multiple-comparison,and stepwise regression analysis.ResultsThe single scores for behavioral disorders( (2.79 ±2.96),(3.42 ± 2.98 ) vs (2.85± 2.93 ) ),affective disorders ( ( 1.50 ± 1.47 ),( 1.76 ± 1.50 ) vs ( 1.51 ± 1.47 ) ),interpersonal relationship( ( 1.78 ± 1.93 ),( 2.44 ± 2.14) vs (2.30 ± 2.18 ) ),environmental adjustment( (0.63 ± 0.94 ),( 0.89 ± 1.02 )vs ( 1.02 ± 1.18) ),and total score ( ( 6.71 ± 6.01 ),( 8.50 ± 6.20) vs ( 7.69 ± 6.89 ) ) had significant differences in the three decades (P ＜ 0.01 ).The changing disposition of behavior,emotion,interpersonal relationship and total mental maladjustment seemed as reversed V in three decades,but environmental adjustment revealed linear increase; stepwise regression analysis revealed that total scores of mental maladjustment had significant decadeeffect after introducing other four independent variables( t =5.96,P =0.000 ) ; the educational level,urban-rural also had significant influence on military mental maladjustment( t =- 3.15,- 9.02,P =0.002 ～ 0.000 ).ConclusionThe mental maladjustment of military personnel reveale a decreasing disposition from 80s',90s' of last century to the year of 2000 ; the influencing factors are decade,educational level and rural-urban.

Objective To explore the differential expression of lncRNAs in patients with depression and its relationship with personality traits and social support.Methods The differential expression of lncRNAs in 5 patients with depression (MDD) and 5 normal controls (NC) was screened by gene chip.To validate the gene chip dataset,qRT-PCR was used to verify the expression levels of these 10 lncRNAs in a separate set of 138 consecutive patients and 43 normal subjects,and then the relationships of lncRNA expression level with personality traits and social support were analyzed.Results A total of 2 649 lncRNAs were differentially expressed,of which 534 were up-regulated and 2 115 down-regulated.The expression levels of 8 lncRNAs analyzed by qRT-PCR in patients with depression were significantly down-regulated (P＜0.05 or P＜0.01).The △Ct value of PY4 was negatively correlated with anxiety/somatization factor (r=-0.210,P＜0.05),and the △Ct values of PY1,PY2 and PY6 were negatively correlated with the social support availability (r=-0.383,-0.391,-0.381 all P＜0.05).Apart from PY1,the △Ct values of the other lncRNAs were negatively correlated with paranoid (P＜0.05),the △Ct values of PY3,PY6 and PY9 were negatively correlated with the borderline and obsessive-compulsive (P＜0.05) and except for PY10,the △Ct values of the other lncRNAs were negatively correlated with the schizoid (P＜0.05).Conclusion The expression levels of theses 8 lncRNAs are significantly down-regulated in patients with depression,and there is a certain correlation with anxiety/somatization factor,personality traits and social support.

Hepatocellular carcinoma (HCC) is one of the leading causes of cancer death. There is a high risk of recurrence and metastasis after surgery. Anesthesia methods, anesthesia-related drugs and intraoperative anesthesia management can affect the biological behavior of HCC cells or the body's immunity, thus affecting the recurrence and metastasis of HCC. Paying attention to the effect of anesthesia on recurrence and metastasis of HCC and optimizing anesthesia management are expected to improve the long-term survival of patients.

OBJECTIVE To identify differentially expressed microRNA (miRNA) in peripheral blood mononuclear cells (PBMCs) of anxiety patients and predict their target genes and function by bioinformatics analysis. METHODS The miRNA expression profiles were determined using an Affymetrix array. To validate the results, real-time quantitative polymerase chain reaction (qRT-PCR) analysis in a larger cohort was employed. The targets of the differentially expressed miRNAs were predicted by Target Scan, miRBD, and DIANA-microT-CDS, and the results were analyzed by gene ontology (GO) and KEGG pathway analysis using FunNet. RESULTS MicroRNA microarray chip analysis has identified 7 miRNAs were detected with significant changes in expression in PBMCs of anxiety patients. qRT-PCR analysis has confirmed that the expression levels of 5 miRNAs (has-miR-4484, has-miR-4505, has-miR-4674, has-miR-501-3p and has-miR-663) were up-regulated. Intersecting the genes by Target Scan, miRBD, and DIANA-microT-CDS has predicted 195 targets. GO analysis showed that biological processes regulated by the predicted target genes have included diverse terms. Some terms, e.g., nervous system development, nerve growth factor receptor signaling pathway, neuron migration, dendrite development, regulation of neuron projection development, midbrain development, regulation of excitatory postsynaptic membrane potential, gliogenesis, dendrite morphogenesis, etc. have direct relationship with the central nervous system and brain functions. Pathway analysis showed that a significant enrichment in several pathways related to neuronal brain functions such as glutamatergic synapse, axon guidance, calcium signaling pathway, MAPK signaling pathway, GnRH signaling pathway, Wnt signaling pathway, gap junction, long-term potentiation and VEGF signaling pathway, etc. Among the five microRNAs, has-miR-4484, has-miR-4505, has-miR-4674 and has-miR-501-3p may have more important regulatory functions. CONCLUSION Five miRNAs (has-miR-4484, has-miR-4505, has-miR-4674, has-miR-501-3p and has-miR-663) are up-regulated in PBMCs of anxiety patients and may be closely involved in the pathogenesis of anxiety disorder.
Anxiety Disorders ; genetics ; Computational Biology ; methods ; Gene Expression Regulation ; Humans ; MicroRNAs ; analysis ; Real-Time Polymerase Chain Reaction

Objective To explore the correlation between the long non-coding RNA (lncRNA) expression in peripheral blood mononuclear cell (PBMC) and depressive symptoms in victims of major depression disorder (MDD).Methods A total of 138 consecutive MDD patients who had not taken antidepressant drugs in the last 3 months or were first-episode patients from May 2014 to February 2015 were enrolled in the present study.The expressions of 9 MDD-associated lncRNAs (TCONS l2 000001212,NONHSAT102891,TCONS_00019174,ENST00000566208,NONHSAG045500,ENST00000591189,ENST00000517573,NONHSAT034045 and NONH-SAT142707) in PBMC were determined by real-time fluorescence quantitative PCR,and the severity of the depressive symptoms were evaluated by 24 item Hamiton depressive Scale (HAMD-24).Results The expression level of TCONS L2 00001212 was significantly positively correlated with hopelessness symptoms (r=0.370,P＜0.01),the expression level of TCONS_00019174 was significantly positively correlated with total depressive symptoms,retardation symptoms and hopelessness symptoms (r=0.286,0.346,0.542,all P＜0.01) and the expression level of NONHSAT142707 was significantly positively correlated with total depressive symptoms and hopelessness symptoms (r=0.253,0.525,P＜0.01).The expression level of TCONS_00019174 and NONHSAT142707 in the higher scores subgroup was significantly lower than those in lower scores subgroup(Z=3.238,2.254,P＜0.05).When TCONS_00019174 entered into the regression equation with the total scores as the independent variable,it explained 19.8％ of the variance of total scores.A ROC curve analysis revealed that TCONS_000191745 had moderate intensity prediction function on the severity of the depressive symptoms (AUC=0.833,P＜0.01).When the cutoff value was 8.352 CT,Youden index was 0.495 in maximum with 88.24％ sensitivity and 89.32％ specificity.Conclusion TCONS_00019174 has a good positive prediction performance on symptom severity in MDD patients.