Objective:Discussing the laboratory findings and clinical features of the children with MDS.Methods:Analysing the results of peripheral blood,bone marrow and clinical manifestations in 13 patients with MDS.Results:All 13 patients were male.Main clinical manifestations include fever,anemia and hepatosplenomegaly.The anemia was of moderate to severe degree.Immature RBC were found in the peripheral blood smears.WBC ranged from high,normal or low.Immature peripheral blood cells were seen in 4 patients.The counts of platelets were normal or low.The bone marrow showed hyperplasia actively and abnormal erythropoiesis and/or myelopoiesis.The diagnosis in 10 patients was RA,2 patients,RAEB and 1 patient,RAEB-t.Conclusion:The diagnosis of MDS depends on the bone marrow examination.The bone marrow shows one abnormal hematopoietic system at least and must differentiate with other hematologic disorders.

Objective:To study the relationship between the laboratory behavior and clinical diagnosis as well as therapeutic efficacy of idiopathic thrombocytopenic purpura.Methods:The blood platelet count in peripheral blood,megakaryocyte count and classification in bone marrow smear and chromosome examination in bone marrow were executed respectively in 130 idiopathic thrombocytopenic purpura cases,whose therapeutic efficacy with IVIG and delta prenovis were observed.The blood clot retraction experiment was executed in 89 cases and the collecting function of platelets was detected in 28 cases.Results:There were 5 cases whose chromosome examination was abnormal in all 130 cases.After treated with IVIG and delta prenovis for 6～10 days,clinical symptoms of 89 cases were relieved obviously.Conclusion:The ITP patient who has more megakaryocytes in his bone marrow smear has the better therapeutic efficacy after treated with IVIG and delta prenovis,and the one with the little infantile megakaryocytes has the least therapeutic efficacy.

OBJECTIVE: To analyze the current situation and developing trend of drugs used in 10 hospitals of Hunan Province from 1996 to 2000, and to serve as reference for drug production, marketing and clinical application .METHODS:The sum of money and percentages of different kinds of drugs used each year were added up separately.RESULTS: The sum of money of drugs consumed was increasing at a rate of 18% each year.About 40% of the money was spent on anti - infective drugs and 20% on imported drugs.CONCLUSION: The sum of money of drugs consumed will grow year by year .The clinical use of drugs will be more rational and of more choices,but the sum of money spent on imported drugs will decrease.

Objective To evaluate the merits and demerits of three different closure methods for femoral artery access site in the interventional therapeutic procedures for senile patients with coronary artery disease. Methods We managed the femoral artery access sites in 60 cases undergoing interventional operation using three different hemostasias, including manual compression, Femostop equipment and Perclose suture (20 cases for each group). The procedure time, time for patients staying in bed, expenditure obedience, the percentage of procedure failure and vagal vascular reflex, and local vascular complications were observed and analyzed statistically. Results The study showed that the procedure time in Perclose suture group was about 12 minutes, much shorter than that in finger pressing and Femostop equipment groups (respectively 23 and 55 minutes, P

Objective To investigate the clinical significance of antinucleosome antibody (AnuA) in the patients with lupus erythematosus (LE) through detecting the serum level of AnuA in different types of LE patients before and after treatment. Methods Enzyme-linked immunosorbent assay(ELISA) was used to detect the serum level of AnuA in 31 SLE patients, 26 SCLE patients, 7 DLE patients, 6 scleroderma patients, 5 dermatomyositis patients and 30 healthy controls. Among them, the serum levels of AnuA before and after treatment were detected in 15 SLE patients and 10 SCLE patients. Clinical data, laboratory test results and the scores of SLE disease activity index (SLEDAI) were analyzed with SPSS11.0 software. Results The serum levels of AnuA in SLE patients were higher than those in SCLE, DLE, scleroderma, dermatomyositis patients and healthy controls(P

OBJECTIVETo observe the effects of moxibustion with different temperatures on cholesterol and skin around "Shenque" (CV 8) in mice with acute hyperlipidemia, and to explore the correlation between moxibustion effect and transient receptor potential vanilloid 1 (TRPV1).

METHODSEight mice among 32 mice of C57BL/6J wild type (WT) were selected into a blank group, and the remaining 24 mice were made into the acute hyperlipidemia model by injection of egg yolk. After model establishment, the mice were randomly divided into a model group, a 38 °C moxibustion group and a 46 °C moxibustion group, 8 mice in each group. The temperature was (38 ± 1) °C in the 38 °C moxibustion group and (46 ± 1) °C in the 46 °C moxibustion group. "Shenque" (CV 8) and "Zusanli" (ST 36) were selected in the two groups; moxibustion was given for 10 min per time, once a day for 2 times. Mice in the blank group and model group were treated with immobilization and moxibustion was not given. After treatment, the level of serum cholesterol was tested by oxidase method; the morphology of skin around "Shenque" (CV 8) was observed by HE staining; the expression of TRPV1 in skin was measured by immunohistochemistry.

RESULTS(1) Compared with the blank group, the level of serum cholesterol was increased in the model group (P < 0.001); compared with the model group, the level of serum cholesterol was reduced in the 46 °C moxibustion group (P < 0.001); compared with the 38 °C moxibustion group, the level of serum cholesterol was reduced in the 46 °C moxibustion group (P < 0.01). (2) There was slight change of morphology and structure in skin tissue in the 38 °C moxibustion group, while obvious change was observed in the 46 °C moxibustion group, indicating specific change. (3) The difference of the expression of TRPV1 in skin was significantly different between 46 °C moxibustion group and blank group, model group (both P < 0.01); it was also different from the 38 °C moxibustion group (P < 0.05).

CONCLUSIONMoxibustion temperature is one of the important factors affecting the cholesterol and acupoint skin. The effects of moxibustion are related to TRPV1.

Acupuncture Points ; Animals ; Cholesterol ; blood ; Female ; Humans ; Hyperlipidemias ; blood ; therapy ; Mice ; Mice, Inbred C57BL ; Moxibustion ; Skin ; anatomy & histology ; metabolism ; TRPV Cation Channels ; metabolism ; Temperature

OBJECTIVE: To explore the expression and significance of programmed cell death 5 (PDCD5) and Bcelllymphoma/lewkmia-2(Bcl-2) in sinonasal squamous cell carcinoma (SNSCC). METHOD: Immunohistochemical method and Western Blot method was used to determine the expression of PDCD5 and Bcl-2 in specimen of SNSCC in thirty cases, sinonasal inverted papillomas (SNIP) in thirty-eight cases, and normal nasal mucosa in twenty cases. RESULT: (1) The expression of PDCD5 protein in SNSCC significantly decreased compared with SNIP and normal nasal mucosa. (2) The expression of Bcl-2 protein in SNSCC up-regulated obviously compared with SNIP and normal nasal mucosa. (3) Positive rate of PDCD5 protein and Bcl-2 protein in well, moderate and low differentiatied group is respectively 100.00%, 83.33%, 38.89% and 50.00%, 70.83% and 100.00%, the difference was statistically significant (P < 0.05). (4) In the follow-up cases, the survival rate of the patients with higher expression of PDCD5 protein was higher, but that with lower expression of Bcl-2 protein was higher. CONCLUSION The inactivation of PDCD5 protein and the activation of Bcl-2 protein may play an important role in the development of SNSCC, and there are a positive correlation between PDCD5 and Bcl-2 protein in SNSCC, which may be identified as a new therapeutic target.
Apoptosis Regulatory Proteins ; metabolism ; Carcinoma, Squamous Cell ; metabolism ; mortality ; Head and Neck Neoplasms ; metabolism ; mortality ; Humans ; Neoplasm Proteins ; metabolism ; Papilloma, Inverted ; metabolism ; Paranasal Sinus Neoplasms ; metabolism ; Proto-Oncogene Proteins c-bcl-2 ; metabolism ; Squamous Cell Carcinoma of Head and Neck ; Survival Rate

To establish a method to detect cardiac troponin I by using time-resolved fluoroimmunoassay ( TRFIA) and apply to the clinic.Methods:The assay were measured by TRIFA and double antibody sandwich method .Standard protocols were evaluated with the standard curve , the limit of detection , stability, precision and cross reaction .Healthy reference populations and clinical serum specimens were measured to established the reference interval and evaluated the perspective of the clinical application . Results:The standard curve was Y=7485 .878+1400.924 X with a correlation coefficient of 0.999.The limit of detection was 0.052 ng/ml.The intra-and inter-assay coefficients of variation ( CV) were all <10%.Reference values was <0.14 μg/L.The AUC of ROC curve was 0.971 while the sensitivity was 96.45%,the specificity was 91.43% and the accuracy was 95.69%, with 98.45% of positive predictive value and 82.05%of negative predictive value.The correlation coefficient was 0.993 between our proposed method and the commercially available CLIA kits.There was no significant difference in statistics compared with ECG , CK-MB and cTnT ( P>0.05 ).There was significant difference in statistics compared before and after treatment with AMI ( P<0.001 ) .Conclusion: The TRFIA method for detecting cTnI achieves clinical application standards and may be used for the diagnosis and serosurveillance of acute myocardial infarction patients.

Objective:To investigate the effects of Qingluotongbi granule(QLT) on RANKL(receptor activator of nuclear factor-?B ligand) expression in peripheral blood T lymphocytes of RA patients.Methods:RANKL expression of peripheral blood T lymphocyts was examined using flow cytometry.Six cases of RA patients were enrolled with six healthy volunteers as the control.Meanwhile examination for the level of RANKL expression in T cells after incubation in presense of QLT-contained serum was observed.Results:RANKL expression of peripheral blood T lymphocytes was much higher in active state RA patients than in healthy people(P