Objective To establish a sensitive and specific liquid chromatography method for the determination of loganin and morroniside in rat serum containing Liuwei Dihuang Formula(LDF).Methods Loganin and morroniside were extracted from the rat serum using SPE column,then separated on a C_(18)column.A HPLC method for determining serum concentration of mor- roniside and loganin in rats before and after administration LDF was established.Results A good linear calibration curve of lo- ganin and morroniside was obtained.The recovery of the method was in the range of 95 %～105 %.The extracted recovery was more than 75 % ,and within-day and intra-day precisions were less than 12 %.Conclusion This method is sensitive, simple and rapid for monitoring of drug level in the study on pharmacokinetics and different combination of LDF.

Objective To investigate the real experiences of nurses participating in the treatment of patients with new respiratory infectious diseases. Method The semi-structural interview method was used to carry out in-depth interviews with 8 nurses who were involved in the whole process of treatments and cares of patients with SARS and H1N1 influenza to understand their real experiences. Result The nurses, who participated in the treatment and care to patients with new respiratory infectious diseases, experienced such emotions of nervousness, excitement, anxiety, fear, exhaustion, obtained support from their families, hospitals, society and meanwhile recognized their lack of professional knowledge and clinical skills. Conclusion The nurses participating in the treatment of patients with new respiratory infectious diseases should be given strengthened professional training , psychological and social support.

Objective To investigate the expression and clinical value of miR-127-3p in plasma of patients with breast cancer .Methods 80 cases of breast patients , 70 cases of benign breast tumor patients and 70 cases of normal control group were recruited .A real-time fluorescent quantitative reverse transcription polymerase chain reaction ( RT-PCR ) method for detecting miR-127-3p was established; Liner, reproducibility, and specificity were evaluated.In addition, correlations between the relative expression of plasma miR-127-3p and the concentrations of CEA and CA 153 were assessed.the relationship of miR-127-3p expression and clinicopathological features was further determined by Mann-Whitney test.Results The method for detection of plasma miR-127-3p was established.The relative plasma expression of miR-127-3p in breast patients [ 10.561 ( 5.424 -16.465 ) ] was significantly higher than that in benign breast tumor patients [3.015 (1.987-5.035)] (P=0.000 6) and healthy controls [2.375 (1.173-4.370)] (P=0.000 2).However, there was no significant difference between benign tumor patients and healthy control group (P=0.143).Positive relationship was found between the relative expression of miR-127-3p and the concentration of CA153 (R2 =0.457, P=0.003).The area under the ROC curve (AUC) of miR-127-3pwas 0.763, which was higher than that of CEA and CA 153.No significant difference was found between plasma miR-127-3p expression and clinicalpathological features including tumor size , differentiation and tumor node metastasis stage (P>0.05).Conclusions miR-127-3p was increased in breast cancer patients and may be an important diagnostic index for breast cancer .

Objective To investigate the current situation of maternal breastfeeding after the childbirth in Shanghai and analyze its influencing factors, so as to improve breastfeeding.Methods In this study, longitudinal follow-up and questionnaire designed based on the hospital were carried out on pregnant women at the outpatient department in eight baby-friendly hospitals of Shanghai from October 2015 to January 2016. The questionnaires included demographic characteristics and their prospective feeding method after the childbirth. Pregnant women were followed up until the delivery was finished. At the time of hospitalization discharge,they were given the 2nd questionnaire containing the basic situation after the delivery and the actual breastfeeding behavior situation.Results The rates of pregnant women with the intention of breastfeeding and exclusive breastfeeding during the third trimester were 82.5% and 82.3% respectively. After the delivery,56.4% (399/707) of mothers were breastfeeding. Binary logistic regression analysis showed that,for primipara group, breastfeeding behavior after the delivery was related to five factors:the breastfeeding intention in third trimester, age,household register,the time of onset of lactation and whether the baby was in the care unit;for multiparas group,breastfeeding behavior was related to two factors:the time of onset of lactation and whether the baby was in the care unit.Conclusions Health care workers should attach importance to the promotion of breastfeeding during pregnancy for primipara group and pay attention to the health education of the mothers with registeredpermanent residence of Shanghai. During the hospitalization,health care workers should strictly implement the "early sucking,early contact" as the baby-friendly hospital required,strengthen the breastfeeding knowledge and skill training for mothers whose babies are in the maternal care unit,ensure the normal lactation of mothers without their babies so as to improve the early breast-feeding rate after the delivery.

One hundred ten patients with Parkinson′s disease(PD)and 110 age-matched healthy controls were enrolled in the study.The non-motor symptoms(NMS)were assessed with Non-motor symptoms of Parkinson′s disease questionnaire(PDNMSQ30).The PD patients were classified as gait abnormality posture type(PIGD, n=73)and tremor type(TD, n=37).The non-motor symptoms were compared between two groups.Results showed that the NMS in PD patients was higher than that in healthy controls[(35.2 ±3.0)vs.(6.0 ±2.0)symptoms, t=6.43, P=0.01].The NMS score of PIGD group was higher than that of TD group[(28.9 ±1.1)vs.(6.2 ±0.7)symptoms, t=2.73, P<0.01]; Mini-mental state examination(MMSE)and Montreal cognitive assessment scale(MoCA)scores of TD group were significantly higher than those of PIGD group(t=11.52,P<0.01; t=8.79,P<0.01).The results indicate that for PD patients with different motor types,their NMS are different,and PD patients with PIGD have more axial involvement,and more NMS.

Objective To understand the medication compliance,family care and their correlation in patients with chronic hepatitis B. Methods From January to Decemeber 2016,two hundred and seventy-six chronic hepatitis B patients from a class III grade A infectious disease hospital in Nantong were selected as the research object by convenient sampling method. They were investigated by the medication compliance questionnaire and the family care index questionnaire. Results Chronic hepatitis B patients with good medication compliance accounted for 48.91%,the family care score was(7.82±2.38)points. Pearson correlation analysis showed negative correlation between the medication compliance of patients and family care(r=-0.173, P < 0.05). There were significance differences in medication compliance among different age,gender, occupation,cultural level,monthly income,medical payment(χ2=27.45,8.42,13.36,24.76,10.65,18.38;P < 0.05). There were significant differences in family care scores among patients of different age,gender, occupation,marital status,monthly income and method of medical payment(t=7.41,2.52,4.96,6.88,5.65, 11.26;P<0.05). Conclusions The medication compliance and family care of chronic hepatitis B patients still needs to be further improved. It is necessary to strengthen the multi family-oriented nursing intervention,and improve medication compliance and family care so as to delay progression and improve quality of life of patients.

Objective To explore the negative emotion in decompensated cirrhosis patients with HBV and its influencing factors. Methods A total of 251 decompensated cirrhosis patients with HBV of hepatic department in Nantong Third People's Hospital,the Affiliated Hospital of Nantong University,were extracted from January 2016 to December 2016. They were investigated with the self-rating depressive scale(SDS)and the self rating anxiety scale(SAS). Results The scores of SDS and SAS of 251 decompensated cirrhosis patients with HBV were(57.34±12.44)and(52.48±8.98). The incidence rates of depression and anxiety were 66.53% and 56.27%. Pearson correlation analysis showed that there was a significantly positive correlation between the scores of SDS and SAS(r=0.491,P < 0.001). There were statistically significant differences in the scores of SDS and SAS among different ages,sexes,occupations,cultural levels,marital status,monthly income(P < 0.05). There were statistically significant differences in the incidence rates of depression and anxiety among ages,sexes, occupations,cultural levels,marital status,monthly income,medical payment(P<0.05). Conclusions The scores of SDS and SAS as well as the incidence rates of depression and anxiety are high in decompensated cirrhosis patients with HBV. It should be paid high attention to. Comprehensive measures should be taken to relieve depression and anxiety.

Objective To explore and analyze the social support and family burden in patients with chronic hepatitis B (CHB).Methods From January to December 2016, a total of 272 patients with CHB and 272 primary caregivers in a Class Ⅲ Grade A hospital in Nantong were selected by convenience sampling method, and investigated by social support rating scale (SSRS) and family burden scale of disease (FBS). The results were statistically analyzed by SPSS 17.0 software, and the correlation between social support and family burden was analyzed by Pearson correlation analysis.Results The score of social support was (44.99±7.21) and family burden was (16.59±11.03). Pearson correlation analyzsis showed a negative correlation between social support and family burden (r=-0.176,P=0.004). The differences of social support were statistically significant among CHB patients with different marital status, monthly income and medical payment method (t/F=5.499, 11.490, 23.140;P<0.05). The differences of family burden were statistically significant among CHB patients' caregivers with different gender, occupation, education level, relation with patients and time caregiving (t/F= 2.412, 2.180, 7.990, 2.714, 6.372;P<0.05).Conclusions Further interventions should be implemented to improve social support and reduce disease burden for patients with CHB.

Objective To explore the efficacy of group intervention on the medication compliance, the degree of family care, negative emotion in patients with chronic hepatitis B. Methods From July to August 2016, a total of 80 patients with chronic hepatitis B who volunteered to take part in this research were selected and divided into two groups by random table method, with 40 cases in each group. The experimental group received group intervention, while the control group were given routine intervention. The medication compliance, the degree of family care and the negative emotion of the two groups were compared before and after intervention. Results After 6 months' intervention, the medication compliance of the experimental group was (0.67±0.85), and that of the control group was (1.20±1.34). The difference was statistically significant (t=2.097, P< 0.05). Patients with good family function in the experimental group increased from 65.00% to 87.50%. The scores of SAS and SDS were (42.92±9.04) and (45.91±8.89), which were both lower than those of the control group, with statistical significance (t=2.231, 2.226;P< 0.05). Conclusions Group intervention could help increase medication compliance and family care degree, at the same time decrease negative emotion in patients with chronic hepatitis B.

Objective To evaluate the efficacy and safety of Qingpeng ointment for the treatment of localized eczema in children.Methods A multicenter,randomized,open-labeled,tacrolimus 0.03％ ointment-controlled clinical trial was conducted.A total of 442 children with localized eczema were enrolled into this study,and divided into 2 groups to topically apply Qingpeng ointment (Qingpeng group) and tacrolimus 0.03％ ointment (tacrolimus group) respectively twice a day for 2 weeks.The children were followed up before the treatment and 1,2 weeks after the treatment,and the efficacy and safety were evaluated at the same time.Results Clinical data from 426 children were included in the efficacy analysis.At 1,2 weeks after the treatment,there were no significant differences in the response rate between the Qingpeng group and tacrolimus group (1 week after the treatment:78.92％ vs.81.77％,x2 =0.545,P =0.460;2 weeks after the treatment:98.65％ vs.97.54％,x2 =0.721,P =0.396).However,the pruritus scores at 1,2 weeks after the treatment were both significantly lower in the Qingpeng group than in the tacrolimus group (1 week:F =14.001,P =0.000;2 weeks:F =11.252,P =0.001).At 1 week after the treatment,the incidence rate of adverse reactions was significantly lower in the Qingpeng group than in the tacrolimus group (P ＜ 0.05).Mild itching,burning sensation and other local irritant sensations were the most common adverse reactions.Conclusion Qingpeng ointment is safe and effective for the treatment of localized eczema in children with good tolerability.