Objective: To explore the underlying mechanism by which acupuncture regulates the peripheral leukocyte count and observe the relationship between the effects of electroacupuncture (EA) and the spleen ultrastructure in leukopenia. Methods: Leukopenia models of rabbits were established by injecting cyclophosphamide (CY) into rabbits' ear vein,and then the rabbits were treated with acupuncture. The peripheral leukocyte count and classification were measured daily. At last, after the animals were anesthesized, the abdominal cavity was opened, and a piece of spleen tissue was cut. The diameter of splenic sinusoid basal lamina eyehole was measured under electric microscope. Results: The peripheral blood leukocyte count in the EA group increased significantly with shift to right of the granulocyte nuclei compared with model control group (P＜0.01). Moreover, the calibers of splenic sinusoid basal lamina eyehole in the EA group were larger than those in the model control group (P＜0.05). Conclusion: Acupuncture can enhance the peripheral leukocyte count by promoting spleen activity in the early phase of leukopenia.

Objective To evaluate the image quality,diagnosis accuracy and dose reduction of split-bolus CT urography (CTU) with low voltage scan and sinogram affirmed iterative reconstruction (SAFIRE).Methods A total of 80 cases of consecutive patients with confirmed or suspected urinary system disease needed CTU examination were divided into two groups (control group and test group) by using random number table.In control group,convention scan (120 kV) with one time injection was used.But low voltage scan (80 kV) with SAFIRE algorithm and split-bolus injection (SBI) was used in experiment group.The radiation dose,image quality and diagnosis accuracy were compared.Results A total of 77 cases completed CTU examination successfully in the two groups,including 39 cases in control group and 38 cases in test group.The effective dose reduced from (26.68 ± 4.07) in control group to (3.93 ± 0.85) mSv in test group (t =-33.78,P ＜ 0.05).Subjective image quality score was (4.49 ± 0.79) in control group and (4.39 ± 1.53) in test group,with no significantly statistical difference (Z =2.71,P ＞ 0.05).Signal-to-noise ratio (SNR) of objective image quality in test group was higher than that in control group (127.3±15.9 vs.109.6 ± 13.2,t =4.49,P＜0.05).But there was no significantly statistical difference in contrast-to-noise ratio (CNR) between control group(100.8 ± 12.9)and test group (109.0 ± 14.4,P ＞ 0.05).For diagnosis accuracy,no statistical difference were found between two groups(84.62％ and 81.58％,P ＞ 0.05).Conclusions The combination of low voltage scan with SAFIRE algorithm and split-bolus injection CTU could reduce the radiation dose significantly,but the objective image quality,CNR (except SNR) of subjective image quality and diagnosis accuracy were all unaffected obviously.

Background In recent years,some researches had been conducted on the pathologic changes of the secondary injury of perihematoma in animal experiments,but only a few studies had been done on the dynamic pathologic and ultrastructural changes of the perihematoma in ICH patients. The unique contribution of our study is to investigate the dynamic pathologic and ultrastructural changes of the perihematoma in ICH patients and provide significant insights into how the pathophysiology and ultrastructures changed after ICH.Methods The written informed consents were obtained from the ICH patients or their relatives. 30 patients (the supertentorial hemotoma volume＞30 mi and the cerebellar hemotoma volume ＞10 mi) were divided into 8 groups according to the time passed after ICH:＜6 h (6 patients), 6 ～ 12 h (7 patients), 12 ～24 h (5 patients), 24～48 h (3 patients), 48 ～72 h (3 patients), 3 ～4 days group (3 patients), 5 days group (2 patients) and 8 days group ( 1 patient) and subjected to craniotomy for hemotoma evacuation. During the operation for the hemotoma's evacuation, a small amount of tissues that must be removed, which located at 1 cm near the hematoma, were taken as experimental groups; And the same tissues of 7 patients (＜12 h), which were far from the hemotoma on the operational way, were taken as control group. The pathologic and ultrastructral changes were observed.Results The tissues of the control group were almost normal while the damages of the tissues from the experimental groups were slight in ＜6 h groups, more severe after 6h and got to the maximum between 24 ～48 h , recovered gradually after 72 h, became similar to the 6 ～ 12 h group on 5 th day, got better on 8 th day and resembled the 6 h group.Conclusions The injury of the perihematoma occurred in early stage, reached the peak level between 24 and 48 hours after ICH; which was consistent to the clinical nervous functional deficits in the ICH patients.

Objective:To evaluate the feasibility of low-voltage, low-concentration, and low-volume iodine contrast agent for coronary artery CT imaging in patients with chronic kidney disease (CKD).Methods:A total of 36 patients from July 2016 to January 2018 with CKD who were suspected of having coronary artery disease (CAD) were selected prospectively and were performed with coronary artery CT angiography (CCTA) by randomly divided into control group and observation group. In the control group, 13 patients were scanned with a conventional tube voltage of 120 kV, a step-and-shot (SAS) scanning protocol, 63-85 ml injection of a high-concentration iodine contrast agent (iopromide, 370 mg I/ml), and traditional filtered back projection (FBP) reconstruction algorithm. In the observation group, 23 patients were scanned with a low-voltage SAS scanning scheme based on body mass index (BMI) (100 kV for BMI ≥ 25 kg/m 2 or 80 kV for BMI<25 kg/m 2), isotonic low-concentration iodine agent(iodixanol, 270 mgI/ml), low volume injection (45 ml) and iterative algorithm reconstruction algorithm. The scans for both groups of patients were turned on automatic tube current modulation (4D CareDose). The coronary artery image quality of the two groups was evaluated with subjective and objective method. The radiation dose was calculated based on the product of the dose length product (DLP) and the chest coefficient k. The total iodine intake of the two groups was calculated by the product of the iodine concentration and the injection volume. Changes in renal function were calculated as a percentage decrease in serum creatinine clearance before and on day 3 after examination. Results:Totally 32 patients were performed CCTA successfully. Subjective scores of coronary artery image quality in the control and observation groups were 3.789±0.598 and 3.708±0.717, respectively, with no significant statistically difference( P>0.05). The signal noise ratios were 12.88±4.53 and 13.67±2.08(LAD), 11.9±5.0 and 12.6±5.1(RCA), 12.78±3.15 and 13.22±3.10(LCX), with no significant statistically difference ( P>0.05). The average contrast noise ratios were 10.94±1.31 and 11.27±1.81 (LAD), 10.38±1.90 and 11.01±2.26 (RCA), 11.71±3.15 and 12.49±3.62 (LCX), with no significant statistically difference ( P> 0.05). The average radiation doses in observation and control group were (1.09±0.19) and (2.85±0.59) mSv, respectively, with statistically significant difference between the two groups ( t=20.260, P<0.05). The average iodine intakes of the control and observation group were (2.71±0.37) and (1.22±0.00) g, respectively, with statistically significant difference ( t=18.162, P<0.05). The decreases of serum creatinine clearance within 72 hours after injection of iodine contrast agent in the control group and observation group were (28.75±5.24)% and (11.89±4.98)%, respectively, with significant statistically difference ( χ2=9.004, P<0.05). Conclusions:Triple-low scanning scheme can reduce the total iodine consumption and radiation dose of patients with little effect on renal function and meeting the diagnostic requirements of image quality. It is suitable for CCTA of patients with CKD.

Objective To analyze the CT and MRI features of splenic inflammatory myofibroblastoma (SIMF).Methods The CT and MRI images of 6 patients with SIMF were retrospectively analyzed.Results 5 cases underwent CT examination;2 cases did MRI.Six cases of SIMF were all single lesions;the average diameter was (3.9 ± 0.9) cm;the lesions were round or gourdshaped;most have clear boundaries with uneven scan density.On the MRI,the parenchymal part was slightly low signal on T1WI,inhomogeneous hypointensity on T2WI,or high signal on T2WI.After CT or MRI enhancement,lesions were progressively enhanced.Correct preoperative diagnosis was established in only 1 patient.Conclusion In this study,all SIMF cases present as single lesion.The circular appearance and clear boundaries are in common.Asymptotic intensification with map-unenhanced regions is the main enhancement feature.