1.Effects of paeonol on the function of bone marrow-derived macrophage from Porphyromonas gingivalis-induced mice.
West China Journal of Stomatology 2017;35(2):139-144
OBJECTIVEThis work aims to examine the effects of paeonol treatment on the ability of bone marrow-derived macrophage (BMM) to excrete inflammatory factors and to differentiate into osteoclasts upon induction with Porphyromonas gingivalis (P. gingivalis). This work also aims to investigate the underlying mechanisms of these abilities.
METHODSBMM culture was treated with different paeonol concentrations at for 1 h and then stimulated with P. gingivalis for 24 h before programmed death-ligand 1 (PD-L1) was quantified with flow cytometry. Tumor necrosis factor-α (TNF-α), interleukin (IL)-1β, and IL-6 were detected by enzyme-linked immunosorbent assay (ELISA). The BMM culture was treated with the receptor activator for nuclear factor-κB ligand (RANKL) and macrophage colony-stimulating factor (M-CSF), and then with paeonol for 1 h prior to induction with P. gingivalis. Then, osteoclast formation was assessed using tartrate resistant acid phosphatase (TRAP) staining. The osteoclast-related proteins TRAP and receptor activator of nuclear factor-κB (RANK) were quantified by Western blotting.
RESULTSPaeonol was nontoxic to BMM within a range of 10-50 μmol·L⁻¹. Flow cytometry showed that paeonol inhibited PD-L1 expression in P. gingivalis-induced BMM in a dose-dependent manner. ELISA indicated that paeonol dose-dependently inhibited the excretion of TNF-α, IL-1β, and IL-6 by P. gingivalis-induced BMM (P<0.01). TRAP staining revealed that paenol treatment inhibited the differentiation of P. gingivalis-induced BMM into osteoclasts. Western blot results suggested that paeonol decreased the expression of TRAP and RANK in BMM.
CONCLUSIONSPaeonol dose-dependently inhibited the excretion of the inflammatory factors TNF-α, IL-1β, and IL-6 by P. gingivalis-induced BMM in a dose-dependent manner. Moreover, paenol treatment prevented the differentiation of P. gingivalis-induced BMM differentiation into osteoclasts. .
Acetophenones ; pharmacology ; Acid Phosphatase ; Animals ; Carrier Proteins ; Cell Differentiation ; Interleukin-1beta ; Interleukin-6 ; Isoenzymes ; Macrophage Colony-Stimulating Factor ; Macrophages ; Membrane Glycoproteins ; Mice ; Osteoclasts ; Porphyromonas gingivalis ; RANK Ligand ; Receptor Activator of Nuclear Factor-kappa B ; Tumor Necrosis Factor-alpha
2.Salary reforms under high-quality development of public hospitals:taking a public hospital as an example
Haozhe HUANG ; Guoguan ZHENG ; Lingkai SU ; Qi GUO ; Guoli YANG
Modern Hospital 2024;24(5):773-775,779
A high-level performance management system is indispensable for supporting the high-quality development of public hospitals.Under the background of comprehensive overhaul in the remuneration mechanisms within public hospitals and promotion of the contemporary management system of public hospitals,an increasing number of these hospitals are embracing per-formance-driven reform.Through a systematic analysis of the current issues in the remuneration system reform of public hospitals,this paper conducts a series of reform measures implemented within these public hospital to underscore the beneficial impact of ef-fective performance appraisal andremuneration incentives on the morale of medical staff and the sustainable cultivation of talent.This paper seeks to offer insights into enhancing remuneration system reforms and fostering high-quality development in public hospitals in the contemporary era.